Aluminum-induced high IAA concentration may explain the Al susceptibility in Citrus limonia
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s10725-018-0458-5 http://hdl.handle.net/11449/189674 |
Resumo: | In acidic soils (pH < 5.0), aluminum (Al) occurs as Al 3+ , which is phytotoxic and reduces the root growth by hormonal imbalance and/or cell wall rigidity. However, the explanations for the decrease in root growth are not clear. A 60-day study was held with ‘Rangpur’ lime (Citrus limonia) plants grown in nutrient solution containing 0 and 1480 µM Al. We measured plant biometric data and used root apices to analyze auxin (IAA), the expression of some Al-responsive genes that had been differentially expressed in a transcriptome analysis and anatomical profiles. We found up-regulated expression of multidrug and toxic compound exudation (Cl-MATE channel), citrate synthase (Cl-CS) and pectin methylesterase inhibitor (Cl-PMEI) genes, but while Cl-PMEI expression was increased after 7 days, Cl-CS and Cl-MATE were up-regulated only after 60 days, suggesting that the timing of these events was ineffective against Al. These results could be associated with the Al-induced decrease in root growth and anatomical root damage. In addition, genes related to IAA cell transport were not differentially expressed in the transcriptome analysis. Therefore, high IAA and up-regulation of auxin-related small RNAs suggest Al-induced high IAA concentration rather than Al-induced disruption in IAA distribution in root cells of this species. |
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Aluminum-induced high IAA concentration may explain the Al susceptibility in Citrus limoniaAl 3+AuxinEfflux channelsGene expressionRoot growth‘Rangpur’ limeIn acidic soils (pH < 5.0), aluminum (Al) occurs as Al 3+ , which is phytotoxic and reduces the root growth by hormonal imbalance and/or cell wall rigidity. However, the explanations for the decrease in root growth are not clear. A 60-day study was held with ‘Rangpur’ lime (Citrus limonia) plants grown in nutrient solution containing 0 and 1480 µM Al. We measured plant biometric data and used root apices to analyze auxin (IAA), the expression of some Al-responsive genes that had been differentially expressed in a transcriptome analysis and anatomical profiles. We found up-regulated expression of multidrug and toxic compound exudation (Cl-MATE channel), citrate synthase (Cl-CS) and pectin methylesterase inhibitor (Cl-PMEI) genes, but while Cl-PMEI expression was increased after 7 days, Cl-CS and Cl-MATE were up-regulated only after 60 days, suggesting that the timing of these events was ineffective against Al. These results could be associated with the Al-induced decrease in root growth and anatomical root damage. In addition, genes related to IAA cell transport were not differentially expressed in the transcriptome analysis. Therefore, high IAA and up-regulation of auxin-related small RNAs suggest Al-induced high IAA concentration rather than Al-induced disruption in IAA distribution in root cells of this species.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Programa de Pós-Graduação em Ciências Biológicas (Biologia Vegetal) Instituto de Biociências Departamento de Botânica Universidade Estadual Paulista UNESP, Av. 24-A, 1515Departamento de Alimentos e Nutrição Experimental/NAPAN/FoRC-Food Research Center Faculdade de Ciências Farmacêuticas Universidade de São Paulo USP, Av. Prof. Lineu Prestes, 580, bl 14Centro de P&D de Sanidade Vegetal Laboratório de Bioquímica Fitopatológica Instituto Biológico, Av. Conselheiro R. Alves, 1252Departamento de Fitopatologia e Nematologia Escola Superior de Agricultura “Luiz de Queiróz” - Universidade de São Paulo ESALQ-USP, Av. Pádua Dias, 11Departamento de Botânica Instituto de Biociências Universidade Estadual Paulista UNESP, Av. 24-A, 1515Programa de Pós-Graduação em Ciências Biológicas (Biologia Vegetal) Instituto de Biociências Departamento de Botânica Universidade Estadual Paulista UNESP, Av. 24-A, 1515Departamento de Botânica Instituto de Biociências Universidade Estadual Paulista UNESP, Av. 24-A, 1515FAPESP: 2013/07914-8FAPESP: 2013/11370-3FAPESP: 2014/14386-0FAPESP: 2016/14216-3CNPq: 305458/2013-2CNPq: 309149/2017-7CNPq: 474169/2013-8Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Instituto BiológicoSilva, Carolina M. S. [UNESP]Cavalheiro, Mariana F. [UNESP]Bressan, Anna C. G. [UNESP]Carvalho, Brenda M. O. [UNESP]Banhos, Otavia F. A. A. [UNESP]Purgatto, EduardoHarakava, RicardoTanaka, Francisco A. O.Habermann, Gustavo [UNESP]2019-10-06T16:48:30Z2019-10-06T16:48:30Z2019-01-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article123-137http://dx.doi.org/10.1007/s10725-018-0458-5Plant Growth Regulation, v. 87, n. 1, p. 123-137, 2019.1573-50870167-6903http://hdl.handle.net/11449/18967410.1007/s10725-018-0458-52-s2.0-85057072808Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPlant Growth Regulationinfo:eu-repo/semantics/openAccess2021-10-23T14:26:51Zoai:repositorio.unesp.br:11449/189674Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T14:26:51Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Aluminum-induced high IAA concentration may explain the Al susceptibility in Citrus limonia |
title |
Aluminum-induced high IAA concentration may explain the Al susceptibility in Citrus limonia |
spellingShingle |
Aluminum-induced high IAA concentration may explain the Al susceptibility in Citrus limonia Silva, Carolina M. S. [UNESP] Al 3+ Auxin Efflux channels Gene expression Root growth ‘Rangpur’ lime |
title_short |
Aluminum-induced high IAA concentration may explain the Al susceptibility in Citrus limonia |
title_full |
Aluminum-induced high IAA concentration may explain the Al susceptibility in Citrus limonia |
title_fullStr |
Aluminum-induced high IAA concentration may explain the Al susceptibility in Citrus limonia |
title_full_unstemmed |
Aluminum-induced high IAA concentration may explain the Al susceptibility in Citrus limonia |
title_sort |
Aluminum-induced high IAA concentration may explain the Al susceptibility in Citrus limonia |
author |
Silva, Carolina M. S. [UNESP] |
author_facet |
Silva, Carolina M. S. [UNESP] Cavalheiro, Mariana F. [UNESP] Bressan, Anna C. G. [UNESP] Carvalho, Brenda M. O. [UNESP] Banhos, Otavia F. A. A. [UNESP] Purgatto, Eduardo Harakava, Ricardo Tanaka, Francisco A. O. Habermann, Gustavo [UNESP] |
author_role |
author |
author2 |
Cavalheiro, Mariana F. [UNESP] Bressan, Anna C. G. [UNESP] Carvalho, Brenda M. O. [UNESP] Banhos, Otavia F. A. A. [UNESP] Purgatto, Eduardo Harakava, Ricardo Tanaka, Francisco A. O. Habermann, Gustavo [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) Instituto Biológico |
dc.contributor.author.fl_str_mv |
Silva, Carolina M. S. [UNESP] Cavalheiro, Mariana F. [UNESP] Bressan, Anna C. G. [UNESP] Carvalho, Brenda M. O. [UNESP] Banhos, Otavia F. A. A. [UNESP] Purgatto, Eduardo Harakava, Ricardo Tanaka, Francisco A. O. Habermann, Gustavo [UNESP] |
dc.subject.por.fl_str_mv |
Al 3+ Auxin Efflux channels Gene expression Root growth ‘Rangpur’ lime |
topic |
Al 3+ Auxin Efflux channels Gene expression Root growth ‘Rangpur’ lime |
description |
In acidic soils (pH < 5.0), aluminum (Al) occurs as Al 3+ , which is phytotoxic and reduces the root growth by hormonal imbalance and/or cell wall rigidity. However, the explanations for the decrease in root growth are not clear. A 60-day study was held with ‘Rangpur’ lime (Citrus limonia) plants grown in nutrient solution containing 0 and 1480 µM Al. We measured plant biometric data and used root apices to analyze auxin (IAA), the expression of some Al-responsive genes that had been differentially expressed in a transcriptome analysis and anatomical profiles. We found up-regulated expression of multidrug and toxic compound exudation (Cl-MATE channel), citrate synthase (Cl-CS) and pectin methylesterase inhibitor (Cl-PMEI) genes, but while Cl-PMEI expression was increased after 7 days, Cl-CS and Cl-MATE were up-regulated only after 60 days, suggesting that the timing of these events was ineffective against Al. These results could be associated with the Al-induced decrease in root growth and anatomical root damage. In addition, genes related to IAA cell transport were not differentially expressed in the transcriptome analysis. Therefore, high IAA and up-regulation of auxin-related small RNAs suggest Al-induced high IAA concentration rather than Al-induced disruption in IAA distribution in root cells of this species. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T16:48:30Z 2019-10-06T16:48:30Z 2019-01-15 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s10725-018-0458-5 Plant Growth Regulation, v. 87, n. 1, p. 123-137, 2019. 1573-5087 0167-6903 http://hdl.handle.net/11449/189674 10.1007/s10725-018-0458-5 2-s2.0-85057072808 |
url |
http://dx.doi.org/10.1007/s10725-018-0458-5 http://hdl.handle.net/11449/189674 |
identifier_str_mv |
Plant Growth Regulation, v. 87, n. 1, p. 123-137, 2019. 1573-5087 0167-6903 10.1007/s10725-018-0458-5 2-s2.0-85057072808 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Plant Growth Regulation |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
123-137 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799965618236030976 |