Cyclophosphamide modulated the foreign body inflammatory reaction in tilapia (Oreochromis niloticus)
Main Author: | |
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Publication Date: | 2020 |
Other Authors: | , , , , , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Institucional da UNESP |
Download full: | http://dx.doi.org/10.1016/j.fsi.2020.09.039 http://hdl.handle.net/11449/205312 |
Summary: | In order to understand events and mechanisms present in the pathophysiology of tilapia's chronic inflammation and based on the immunomodulatory activity attributed to cyclophosphamide which is widely used to suppress immune responses in human medicine, the present study investigated the effects of cyclophosphamide (CYP) treatment on the modulation of foreign body inflammatory reaction in Nile tilapia (Oreochromis niloticus) with round glass coverslip implanted in the subcutaneous tissue (9 mm of diameter). Forty tilapia (151 ± 10,2 g) were randomly distributed in 5 aquariums (n = 8) with a capacity of 250 L of water each, to compose two treatments (sampled 3 and 6 days post-implantation): implanted/untreated (control) and implanted/treated with 200 mg of CYP kg−1 of b.w., through i.p. route. A fifth group (n = 8) was sampled without any stimulus (naive) to obtain reference values. CYP-treated tilapia showed decrease in macrophage accumulation, giant cell formation and Langhans cells on the glass coverslip when compared to control fish. The treatment with CYP resulted in decrease of leukocyte and thrombocyte counts. Decrease in alpha-2-macroglobulin, ceruloplasmin, albumin and transferrin levels, as well as increase in haptoglobin, complement C3 and apolipoprotein A1 were observed in tilapias during foreign body inflammation. Blood levels of complement C3, alpha-2-macroglobulin, ceruloplasmin and transferrin were modulated by treatment with CYP. Therefore, the treatment with 200 mg of CYP kg−1 of b.w. in tilapia resulted in an anti-inflammatory effect by suppressing the dynamics between leukocytes in the bloodstream and macrophage accumulation with giant cell formation in the inflamed focus, as well as by modulating APPs during foreign body reaction. |
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Cyclophosphamide modulated the foreign body inflammatory reaction in tilapia (Oreochromis niloticus)Acute phase proteinsAlkylating agentsChronic inflammationCichlidsGiant cellsInnate immunityLanghans cellsMacrophagesIn order to understand events and mechanisms present in the pathophysiology of tilapia's chronic inflammation and based on the immunomodulatory activity attributed to cyclophosphamide which is widely used to suppress immune responses in human medicine, the present study investigated the effects of cyclophosphamide (CYP) treatment on the modulation of foreign body inflammatory reaction in Nile tilapia (Oreochromis niloticus) with round glass coverslip implanted in the subcutaneous tissue (9 mm of diameter). Forty tilapia (151 ± 10,2 g) were randomly distributed in 5 aquariums (n = 8) with a capacity of 250 L of water each, to compose two treatments (sampled 3 and 6 days post-implantation): implanted/untreated (control) and implanted/treated with 200 mg of CYP kg−1 of b.w., through i.p. route. A fifth group (n = 8) was sampled without any stimulus (naive) to obtain reference values. CYP-treated tilapia showed decrease in macrophage accumulation, giant cell formation and Langhans cells on the glass coverslip when compared to control fish. The treatment with CYP resulted in decrease of leukocyte and thrombocyte counts. Decrease in alpha-2-macroglobulin, ceruloplasmin, albumin and transferrin levels, as well as increase in haptoglobin, complement C3 and apolipoprotein A1 were observed in tilapias during foreign body inflammation. Blood levels of complement C3, alpha-2-macroglobulin, ceruloplasmin and transferrin were modulated by treatment with CYP. Therefore, the treatment with 200 mg of CYP kg−1 of b.w. in tilapia resulted in an anti-inflammatory effect by suppressing the dynamics between leukocytes in the bloodstream and macrophage accumulation with giant cell formation in the inflamed focus, as well as by modulating APPs during foreign body reaction.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Pharmacology Institute of Biomedical Science University of São Paulo (USP)Department of Preventive Veterinary Medicine São Paulo State University (UNESP)Veterinary College Federal University of Rondonia (UNIR)Department of Graduate Program in Health Sciences Federal University of Roraima (UFRR)Laboratório de Bioquímica de Peptídeos UNIFESPLaboratory of Animal Pharmacology and Toxicology Brasil UniversityDepartment of Preventive Veterinary Medicine São Paulo State University (UNESP)FAPESP: 2012/12256-7FAPESP: 2013/11675-9CNPq: 432830/2016-2CNPq: 477816/2013-4Universidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Federal University of Rondonia (UNIR)Federal University of Roraima (UFRR)Universidade Federal de São Paulo (UNIFESP)Brasil UniversityCharlie-Silva, IvesConde, Gabriel [UNESP]Mendonça Gomes, Juliana MoreiraJohnny da Rosa Prado, Ed [UNESP]Fernandes, Dayanne Carla [UNESP]Cristina de Moraes, AlessandraEto, Silas FernandesConceição, KatiaAntonio de Andrade Belo, Marco [UNESP]2021-06-25T10:13:13Z2021-06-25T10:13:13Z2020-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article230-237http://dx.doi.org/10.1016/j.fsi.2020.09.039Fish and Shellfish Immunology, v. 107, p. 230-237.1095-99471050-4648http://hdl.handle.net/11449/20531210.1016/j.fsi.2020.09.0392-s2.0-85092606075Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFish and Shellfish Immunologyinfo:eu-repo/semantics/openAccess2021-10-23T12:31:40Zoai:repositorio.unesp.br:11449/205312Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T12:31:40Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Cyclophosphamide modulated the foreign body inflammatory reaction in tilapia (Oreochromis niloticus) |
title |
Cyclophosphamide modulated the foreign body inflammatory reaction in tilapia (Oreochromis niloticus) |
spellingShingle |
Cyclophosphamide modulated the foreign body inflammatory reaction in tilapia (Oreochromis niloticus) Charlie-Silva, Ives Acute phase proteins Alkylating agents Chronic inflammation Cichlids Giant cells Innate immunity Langhans cells Macrophages |
title_short |
Cyclophosphamide modulated the foreign body inflammatory reaction in tilapia (Oreochromis niloticus) |
title_full |
Cyclophosphamide modulated the foreign body inflammatory reaction in tilapia (Oreochromis niloticus) |
title_fullStr |
Cyclophosphamide modulated the foreign body inflammatory reaction in tilapia (Oreochromis niloticus) |
title_full_unstemmed |
Cyclophosphamide modulated the foreign body inflammatory reaction in tilapia (Oreochromis niloticus) |
title_sort |
Cyclophosphamide modulated the foreign body inflammatory reaction in tilapia (Oreochromis niloticus) |
author |
Charlie-Silva, Ives |
author_facet |
Charlie-Silva, Ives Conde, Gabriel [UNESP] Mendonça Gomes, Juliana Moreira Johnny da Rosa Prado, Ed [UNESP] Fernandes, Dayanne Carla [UNESP] Cristina de Moraes, Alessandra Eto, Silas Fernandes Conceição, Katia Antonio de Andrade Belo, Marco [UNESP] |
author_role |
author |
author2 |
Conde, Gabriel [UNESP] Mendonça Gomes, Juliana Moreira Johnny da Rosa Prado, Ed [UNESP] Fernandes, Dayanne Carla [UNESP] Cristina de Moraes, Alessandra Eto, Silas Fernandes Conceição, Katia Antonio de Andrade Belo, Marco [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) Federal University of Rondonia (UNIR) Federal University of Roraima (UFRR) Universidade Federal de São Paulo (UNIFESP) Brasil University |
dc.contributor.author.fl_str_mv |
Charlie-Silva, Ives Conde, Gabriel [UNESP] Mendonça Gomes, Juliana Moreira Johnny da Rosa Prado, Ed [UNESP] Fernandes, Dayanne Carla [UNESP] Cristina de Moraes, Alessandra Eto, Silas Fernandes Conceição, Katia Antonio de Andrade Belo, Marco [UNESP] |
dc.subject.por.fl_str_mv |
Acute phase proteins Alkylating agents Chronic inflammation Cichlids Giant cells Innate immunity Langhans cells Macrophages |
topic |
Acute phase proteins Alkylating agents Chronic inflammation Cichlids Giant cells Innate immunity Langhans cells Macrophages |
description |
In order to understand events and mechanisms present in the pathophysiology of tilapia's chronic inflammation and based on the immunomodulatory activity attributed to cyclophosphamide which is widely used to suppress immune responses in human medicine, the present study investigated the effects of cyclophosphamide (CYP) treatment on the modulation of foreign body inflammatory reaction in Nile tilapia (Oreochromis niloticus) with round glass coverslip implanted in the subcutaneous tissue (9 mm of diameter). Forty tilapia (151 ± 10,2 g) were randomly distributed in 5 aquariums (n = 8) with a capacity of 250 L of water each, to compose two treatments (sampled 3 and 6 days post-implantation): implanted/untreated (control) and implanted/treated with 200 mg of CYP kg−1 of b.w., through i.p. route. A fifth group (n = 8) was sampled without any stimulus (naive) to obtain reference values. CYP-treated tilapia showed decrease in macrophage accumulation, giant cell formation and Langhans cells on the glass coverslip when compared to control fish. The treatment with CYP resulted in decrease of leukocyte and thrombocyte counts. Decrease in alpha-2-macroglobulin, ceruloplasmin, albumin and transferrin levels, as well as increase in haptoglobin, complement C3 and apolipoprotein A1 were observed in tilapias during foreign body inflammation. Blood levels of complement C3, alpha-2-macroglobulin, ceruloplasmin and transferrin were modulated by treatment with CYP. Therefore, the treatment with 200 mg of CYP kg−1 of b.w. in tilapia resulted in an anti-inflammatory effect by suppressing the dynamics between leukocytes in the bloodstream and macrophage accumulation with giant cell formation in the inflamed focus, as well as by modulating APPs during foreign body reaction. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-01 2021-06-25T10:13:13Z 2021-06-25T10:13:13Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.fsi.2020.09.039 Fish and Shellfish Immunology, v. 107, p. 230-237. 1095-9947 1050-4648 http://hdl.handle.net/11449/205312 10.1016/j.fsi.2020.09.039 2-s2.0-85092606075 |
url |
http://dx.doi.org/10.1016/j.fsi.2020.09.039 http://hdl.handle.net/11449/205312 |
identifier_str_mv |
Fish and Shellfish Immunology, v. 107, p. 230-237. 1095-9947 1050-4648 10.1016/j.fsi.2020.09.039 2-s2.0-85092606075 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Fish and Shellfish Immunology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
230-237 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1797789365571682304 |