Regenerated cellulose sponge as sacrificial template for the synthesis of three-dimensional porous alumina-silica scaffold for tissue engineering
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s10971-022-05990-y http://hdl.handle.net/11449/248119 |
Resumo: | Tissue engineering has emerged as a multidisciplinary field that aims to improve health and quality of life by restoring functions of tissues and organs. Cells and scaffolds are the two major components of tissue engineering. Scaffolds act as a support for cells, thus facilitating cell adhesion, proliferation, morphogenesis, differentiation, and extracellular matrix production. Since three-dimensional (3D) porous scaffolds can better simulate the native 3D architecture of in vivo systems than conventional 2D cultures, they are more appropriate to support tissue regeneration. This study aimed to use regenerated cellulose sponge (RCS) as sacrificial template for the synthesis of three-dimensional porous alumina-silica scaffold (ASS). RCS was coated with boehmite-GPTS (3-glycidoxypropyltrimethoxysilane) solution and dried at 60 °C for 12 h. The coated RCS was converted into porous alumina-silica scaffold via thermal treatment at 500 °C for 4 h in air. The materials were characterized by Fourier transform‐infrared (FT‐IR), X-Ray diffraction (XRD), thermogravimetric analysis (TGA) aluminum-27 and silicon-29 nuclear magnetic resonance (NMR), scanning electron microscopy, and energy dispersive X-ray spectroscopy (SEM-EDS). The MTT metabolism assays were used to evaluate indirectly cytocompatibility and cell proliferation using MCT3T3-E1, HDFa and HaCaT cells. The biological in vitro assays demonstrated that only RCS exhibited toxicity toward HDFa cells, although this behavior has been shown questionable once other studies have reported the non-cytotoxic, mutagenic and genotoxic potential of RCS. We believe that tests including clonogenic and mutagenic assays should be performed using HDFa cells in contact with RCS-derived extract in order to further investigate this behavior. The sponge materials have demonstrated different growth rate and adhesion to three different cell lines evaluated. Therefore, the employed sacrificial template approach presents as a viable alternative to produce 3D scaffolds for tissue engineering. Graphical abstract: [Figure not available: see fulltext.] |
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Regenerated cellulose sponge as sacrificial template for the synthesis of three-dimensional porous alumina-silica scaffold for tissue engineeringAlumina-silicaSacrificial templateScaffoldTissue engineeringTissue engineering has emerged as a multidisciplinary field that aims to improve health and quality of life by restoring functions of tissues and organs. Cells and scaffolds are the two major components of tissue engineering. Scaffolds act as a support for cells, thus facilitating cell adhesion, proliferation, morphogenesis, differentiation, and extracellular matrix production. Since three-dimensional (3D) porous scaffolds can better simulate the native 3D architecture of in vivo systems than conventional 2D cultures, they are more appropriate to support tissue regeneration. This study aimed to use regenerated cellulose sponge (RCS) as sacrificial template for the synthesis of three-dimensional porous alumina-silica scaffold (ASS). RCS was coated with boehmite-GPTS (3-glycidoxypropyltrimethoxysilane) solution and dried at 60 °C for 12 h. The coated RCS was converted into porous alumina-silica scaffold via thermal treatment at 500 °C for 4 h in air. The materials were characterized by Fourier transform‐infrared (FT‐IR), X-Ray diffraction (XRD), thermogravimetric analysis (TGA) aluminum-27 and silicon-29 nuclear magnetic resonance (NMR), scanning electron microscopy, and energy dispersive X-ray spectroscopy (SEM-EDS). The MTT metabolism assays were used to evaluate indirectly cytocompatibility and cell proliferation using MCT3T3-E1, HDFa and HaCaT cells. The biological in vitro assays demonstrated that only RCS exhibited toxicity toward HDFa cells, although this behavior has been shown questionable once other studies have reported the non-cytotoxic, mutagenic and genotoxic potential of RCS. We believe that tests including clonogenic and mutagenic assays should be performed using HDFa cells in contact with RCS-derived extract in order to further investigate this behavior. The sponge materials have demonstrated different growth rate and adhesion to three different cell lines evaluated. Therefore, the employed sacrificial template approach presents as a viable alternative to produce 3D scaffolds for tissue engineering. Graphical abstract: [Figure not available: see fulltext.]Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)University of Araraquara—UNIARA, Rua Carlos Gomes 1217, SPDepartment of Chemistry Faculdade de Filosofia Ciências e Letras de Ribeirão Preto University of São Paulo, SPThe School of Pharmaceutical Sciences—FCF Department of Clinical Analysis São Paulo State University—UNESP, Rodovia Araraquara-Jaú, KM 01, SPInstitute of Chemistry São Paulo State University—UNESP, Rua Prof. Francisco Degni 55, SPThe School of Pharmaceutical Sciences—FCF Department of Clinical Analysis São Paulo State University—UNESP, Rodovia Araraquara-Jaú, KM 01, SPInstitute of Chemistry São Paulo State University—UNESP, Rua Prof. Francisco Degni 55, SPUniversity of Araraquara—UNIARAUniversidade de São Paulo (USP)Universidade Estadual Paulista (UNESP)Claro, Amanda MariaAlves, Caroline Cássiados Santos, Kelvin Sousa [UNESP]da Rocha, Euzane Gomesde Lima Fontes, MarinaMonteiro, Gustavo Clarode Carvalho, Gustavo Senra GonçalvesCaiut, José Maurício AlmeidaMoroz, Andrei [UNESP]Ribeiro, Sidney José Lima [UNESP]S. Barud, Hernane2023-07-29T13:35:01Z2023-07-29T13:35:01Z2023-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article83-95http://dx.doi.org/10.1007/s10971-022-05990-yJournal of Sol-Gel Science and Technology, v. 107, n. 1, p. 83-95, 2023.1573-48460928-0707http://hdl.handle.net/11449/24811910.1007/s10971-022-05990-y2-s2.0-85145327268Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Sol-Gel Science and Technologyinfo:eu-repo/semantics/openAccess2023-07-29T13:35:01Zoai:repositorio.unesp.br:11449/248119Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-07-29T13:35:01Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Regenerated cellulose sponge as sacrificial template for the synthesis of three-dimensional porous alumina-silica scaffold for tissue engineering |
title |
Regenerated cellulose sponge as sacrificial template for the synthesis of three-dimensional porous alumina-silica scaffold for tissue engineering |
spellingShingle |
Regenerated cellulose sponge as sacrificial template for the synthesis of three-dimensional porous alumina-silica scaffold for tissue engineering Claro, Amanda Maria Alumina-silica Sacrificial template Scaffold Tissue engineering |
title_short |
Regenerated cellulose sponge as sacrificial template for the synthesis of three-dimensional porous alumina-silica scaffold for tissue engineering |
title_full |
Regenerated cellulose sponge as sacrificial template for the synthesis of three-dimensional porous alumina-silica scaffold for tissue engineering |
title_fullStr |
Regenerated cellulose sponge as sacrificial template for the synthesis of three-dimensional porous alumina-silica scaffold for tissue engineering |
title_full_unstemmed |
Regenerated cellulose sponge as sacrificial template for the synthesis of three-dimensional porous alumina-silica scaffold for tissue engineering |
title_sort |
Regenerated cellulose sponge as sacrificial template for the synthesis of three-dimensional porous alumina-silica scaffold for tissue engineering |
author |
Claro, Amanda Maria |
author_facet |
Claro, Amanda Maria Alves, Caroline Cássia dos Santos, Kelvin Sousa [UNESP] da Rocha, Euzane Gomes de Lima Fontes, Marina Monteiro, Gustavo Claro de Carvalho, Gustavo Senra Gonçalves Caiut, José Maurício Almeida Moroz, Andrei [UNESP] Ribeiro, Sidney José Lima [UNESP] S. Barud, Hernane |
author_role |
author |
author2 |
Alves, Caroline Cássia dos Santos, Kelvin Sousa [UNESP] da Rocha, Euzane Gomes de Lima Fontes, Marina Monteiro, Gustavo Claro de Carvalho, Gustavo Senra Gonçalves Caiut, José Maurício Almeida Moroz, Andrei [UNESP] Ribeiro, Sidney José Lima [UNESP] S. Barud, Hernane |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
University of Araraquara—UNIARA Universidade de São Paulo (USP) Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Claro, Amanda Maria Alves, Caroline Cássia dos Santos, Kelvin Sousa [UNESP] da Rocha, Euzane Gomes de Lima Fontes, Marina Monteiro, Gustavo Claro de Carvalho, Gustavo Senra Gonçalves Caiut, José Maurício Almeida Moroz, Andrei [UNESP] Ribeiro, Sidney José Lima [UNESP] S. Barud, Hernane |
dc.subject.por.fl_str_mv |
Alumina-silica Sacrificial template Scaffold Tissue engineering |
topic |
Alumina-silica Sacrificial template Scaffold Tissue engineering |
description |
Tissue engineering has emerged as a multidisciplinary field that aims to improve health and quality of life by restoring functions of tissues and organs. Cells and scaffolds are the two major components of tissue engineering. Scaffolds act as a support for cells, thus facilitating cell adhesion, proliferation, morphogenesis, differentiation, and extracellular matrix production. Since three-dimensional (3D) porous scaffolds can better simulate the native 3D architecture of in vivo systems than conventional 2D cultures, they are more appropriate to support tissue regeneration. This study aimed to use regenerated cellulose sponge (RCS) as sacrificial template for the synthesis of three-dimensional porous alumina-silica scaffold (ASS). RCS was coated with boehmite-GPTS (3-glycidoxypropyltrimethoxysilane) solution and dried at 60 °C for 12 h. The coated RCS was converted into porous alumina-silica scaffold via thermal treatment at 500 °C for 4 h in air. The materials were characterized by Fourier transform‐infrared (FT‐IR), X-Ray diffraction (XRD), thermogravimetric analysis (TGA) aluminum-27 and silicon-29 nuclear magnetic resonance (NMR), scanning electron microscopy, and energy dispersive X-ray spectroscopy (SEM-EDS). The MTT metabolism assays were used to evaluate indirectly cytocompatibility and cell proliferation using MCT3T3-E1, HDFa and HaCaT cells. The biological in vitro assays demonstrated that only RCS exhibited toxicity toward HDFa cells, although this behavior has been shown questionable once other studies have reported the non-cytotoxic, mutagenic and genotoxic potential of RCS. We believe that tests including clonogenic and mutagenic assays should be performed using HDFa cells in contact with RCS-derived extract in order to further investigate this behavior. The sponge materials have demonstrated different growth rate and adhesion to three different cell lines evaluated. Therefore, the employed sacrificial template approach presents as a viable alternative to produce 3D scaffolds for tissue engineering. Graphical abstract: [Figure not available: see fulltext.] |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T13:35:01Z 2023-07-29T13:35:01Z 2023-07-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s10971-022-05990-y Journal of Sol-Gel Science and Technology, v. 107, n. 1, p. 83-95, 2023. 1573-4846 0928-0707 http://hdl.handle.net/11449/248119 10.1007/s10971-022-05990-y 2-s2.0-85145327268 |
url |
http://dx.doi.org/10.1007/s10971-022-05990-y http://hdl.handle.net/11449/248119 |
identifier_str_mv |
Journal of Sol-Gel Science and Technology, v. 107, n. 1, p. 83-95, 2023. 1573-4846 0928-0707 10.1007/s10971-022-05990-y 2-s2.0-85145327268 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Sol-Gel Science and Technology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
83-95 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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_version_ |
1799965628246786048 |