Proteomic analysis capsule synthesis and redox mechanisms in the intracellular survival of group B Streptococcus in fish microglia
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.fsi.2021.08.019 http://hdl.handle.net/11449/222324 |
Resumo: | Group B Streptococcus (GBS) causes meningitis in neonates and Nile tilapia (Oreochromis niloticus). The molecular mechanisms regulating the intracellular survival of this pathogen in the host cell are complex and crucial for the progression of infection. Thus, we propose the use of GBS-infected Nile tilapia microglia as an in vitro model system simulating infection caused by homologous bacteria in humans. We used this model to evaluate the phagocytic activity, as well as the functional aspects of the capsular proteins A, B, C, and D and the major redox enzymes, and the synergistic role of mechanisms/proteins involved in blocking phagocytic process. We observed that in the intracellular phase, GBS showed enhanced synthesis of the polysaccharide capsule and used superoxide dismutase, thioredoxin, NADH oxidase, and alkyl hydroperoxide reductase to scavenge reactive oxygen species and reactive nitrogen species produced by the host cell. Furthermore, although these virulence mechanisms were effective during the initial hours of infection, they were not able to subvert microglial responses, which partially neutralized the infection. Altogether, our findings provided important information regarding the intracellular survival mechanisms of GBS and perspectives for the production of new drugs and vaccines, through the druggability analysis of specific proteins. In conclusion, tilapia microglia serve as a potent in vitro experimental model for the study of meningitis. |
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Proteomic analysis capsule synthesis and redox mechanisms in the intracellular survival of group B Streptococcus in fish microgliaBrainDruggabilityMeningitisProteomicsVaccineGroup B Streptococcus (GBS) causes meningitis in neonates and Nile tilapia (Oreochromis niloticus). The molecular mechanisms regulating the intracellular survival of this pathogen in the host cell are complex and crucial for the progression of infection. Thus, we propose the use of GBS-infected Nile tilapia microglia as an in vitro model system simulating infection caused by homologous bacteria in humans. We used this model to evaluate the phagocytic activity, as well as the functional aspects of the capsular proteins A, B, C, and D and the major redox enzymes, and the synergistic role of mechanisms/proteins involved in blocking phagocytic process. We observed that in the intracellular phase, GBS showed enhanced synthesis of the polysaccharide capsule and used superoxide dismutase, thioredoxin, NADH oxidase, and alkyl hydroperoxide reductase to scavenge reactive oxygen species and reactive nitrogen species produced by the host cell. Furthermore, although these virulence mechanisms were effective during the initial hours of infection, they were not able to subvert microglial responses, which partially neutralized the infection. Altogether, our findings provided important information regarding the intracellular survival mechanisms of GBS and perspectives for the production of new drugs and vaccines, through the druggability analysis of specific proteins. In conclusion, tilapia microglia serve as a potent in vitro experimental model for the study of meningitis.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Postgraduate in Health Sciences-PROCISA Federal University of Roraima (UFRR)Immunochemistry Laboratory Butantan Institute (CeTICs/FAPESP), Vital Brazil Avenue, 1500Department of Technology School of Agrarian and Veterinary Sciences Sao Paulo State University (Unesp)Immunoregulation Unit of the Laboratory of Applied Toxinology (CeTICs/FAPESP) Butantan Institute, Vital Brazil Avenue, 1500Department of Technology School of Agrarian and Veterinary Sciences Sao Paulo State University (Unesp)Federal University of Roraima (UFRR)(CeTICs/FAPESP)Universidade Estadual Paulista (UNESP)Butantan InstituteEto, Silas FernandesFernandes, Dayanne CarlaBaldassi, Amanda Cristina [UNESP]Balbuena, Thiago Santana [UNESP]da Costa Alecrim, João VictorAlmeida de Carvalho, Fabíola ChristianLima, CarlaLopes-Ferreira, MonicaPizauro, João Martins [UNESP]2022-04-28T19:44:03Z2022-04-28T19:44:03Z2021-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article34-50http://dx.doi.org/10.1016/j.fsi.2021.08.019Fish and Shellfish Immunology, v. 118, p. 34-50.1095-99471050-4648http://hdl.handle.net/11449/22232410.1016/j.fsi.2021.08.0192-s2.0-85114027149Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFish and Shellfish Immunologyinfo:eu-repo/semantics/openAccess2022-04-28T19:44:03Zoai:repositorio.unesp.br:11449/222324Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-28T19:44:03Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Proteomic analysis capsule synthesis and redox mechanisms in the intracellular survival of group B Streptococcus in fish microglia |
title |
Proteomic analysis capsule synthesis and redox mechanisms in the intracellular survival of group B Streptococcus in fish microglia |
spellingShingle |
Proteomic analysis capsule synthesis and redox mechanisms in the intracellular survival of group B Streptococcus in fish microglia Eto, Silas Fernandes Brain Druggability Meningitis Proteomics Vaccine |
title_short |
Proteomic analysis capsule synthesis and redox mechanisms in the intracellular survival of group B Streptococcus in fish microglia |
title_full |
Proteomic analysis capsule synthesis and redox mechanisms in the intracellular survival of group B Streptococcus in fish microglia |
title_fullStr |
Proteomic analysis capsule synthesis and redox mechanisms in the intracellular survival of group B Streptococcus in fish microglia |
title_full_unstemmed |
Proteomic analysis capsule synthesis and redox mechanisms in the intracellular survival of group B Streptococcus in fish microglia |
title_sort |
Proteomic analysis capsule synthesis and redox mechanisms in the intracellular survival of group B Streptococcus in fish microglia |
author |
Eto, Silas Fernandes |
author_facet |
Eto, Silas Fernandes Fernandes, Dayanne Carla Baldassi, Amanda Cristina [UNESP] Balbuena, Thiago Santana [UNESP] da Costa Alecrim, João Victor Almeida de Carvalho, Fabíola Christian Lima, Carla Lopes-Ferreira, Monica Pizauro, João Martins [UNESP] |
author_role |
author |
author2 |
Fernandes, Dayanne Carla Baldassi, Amanda Cristina [UNESP] Balbuena, Thiago Santana [UNESP] da Costa Alecrim, João Victor Almeida de Carvalho, Fabíola Christian Lima, Carla Lopes-Ferreira, Monica Pizauro, João Martins [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Federal University of Roraima (UFRR) (CeTICs/FAPESP) Universidade Estadual Paulista (UNESP) Butantan Institute |
dc.contributor.author.fl_str_mv |
Eto, Silas Fernandes Fernandes, Dayanne Carla Baldassi, Amanda Cristina [UNESP] Balbuena, Thiago Santana [UNESP] da Costa Alecrim, João Victor Almeida de Carvalho, Fabíola Christian Lima, Carla Lopes-Ferreira, Monica Pizauro, João Martins [UNESP] |
dc.subject.por.fl_str_mv |
Brain Druggability Meningitis Proteomics Vaccine |
topic |
Brain Druggability Meningitis Proteomics Vaccine |
description |
Group B Streptococcus (GBS) causes meningitis in neonates and Nile tilapia (Oreochromis niloticus). The molecular mechanisms regulating the intracellular survival of this pathogen in the host cell are complex and crucial for the progression of infection. Thus, we propose the use of GBS-infected Nile tilapia microglia as an in vitro model system simulating infection caused by homologous bacteria in humans. We used this model to evaluate the phagocytic activity, as well as the functional aspects of the capsular proteins A, B, C, and D and the major redox enzymes, and the synergistic role of mechanisms/proteins involved in blocking phagocytic process. We observed that in the intracellular phase, GBS showed enhanced synthesis of the polysaccharide capsule and used superoxide dismutase, thioredoxin, NADH oxidase, and alkyl hydroperoxide reductase to scavenge reactive oxygen species and reactive nitrogen species produced by the host cell. Furthermore, although these virulence mechanisms were effective during the initial hours of infection, they were not able to subvert microglial responses, which partially neutralized the infection. Altogether, our findings provided important information regarding the intracellular survival mechanisms of GBS and perspectives for the production of new drugs and vaccines, through the druggability analysis of specific proteins. In conclusion, tilapia microglia serve as a potent in vitro experimental model for the study of meningitis. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-11-01 2022-04-28T19:44:03Z 2022-04-28T19:44:03Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.fsi.2021.08.019 Fish and Shellfish Immunology, v. 118, p. 34-50. 1095-9947 1050-4648 http://hdl.handle.net/11449/222324 10.1016/j.fsi.2021.08.019 2-s2.0-85114027149 |
url |
http://dx.doi.org/10.1016/j.fsi.2021.08.019 http://hdl.handle.net/11449/222324 |
identifier_str_mv |
Fish and Shellfish Immunology, v. 118, p. 34-50. 1095-9947 1050-4648 10.1016/j.fsi.2021.08.019 2-s2.0-85114027149 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Fish and Shellfish Immunology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
34-50 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797789352757035008 |