Exames parasitológicos, imunoistoquímicos e histopatológicos para detecção de Leishmania chagasi em tecidos esplênicos de cães com leishmaniose visceral
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/225661 |
Resumo: | The purpose of this work was a Canine Visceral Leishmaniasis - CVL study by parasitological direct examination of Leishmania (L.) chagasi (imprinting and histological), immunohistochemical test and histopathological analysis using spleen tissues from 34 dogs euthanized by the Zoonotic Disease Control Centre from Ilha Solteira, SP, Brazil. According to the clinical signs, the dogs were divided in three groups: asymptomatics (8 dogs), oligosymptomatics (17 dogs) and symptomatics (9 dogs). After the accomplishment of all diagnostic tests, 22 dogs were considered positives (64.7%) and 12 (35.3%) were negatives to CVL. From these positive dogs, 1/22 (4.5%) was asymptomatic, 12/22 (54.5%) were oligosymptomatics and 8/22 (40.1%) were symptomatics. The histopathological study in spleen tissues from positive, especially symptomatic dogs, showed a diffuse chronic inflammation with thickness of capsular and trabecular regions and there was extense morphologic alteration of the red and white pulp by the presence of abundant macrophages full with amastigotes, the granulomatous inflammatory reaction and haemorragic areas. The data of this work from histopathologic examination and direct microcopic visualization of L. (L.) chagasi showed that the spleen was an useful organ to collect sample tissues for CVL diagnosis. The immunostaining detected the highest number of positive dogs and were considered an important and conclusive method to be used in addition to parasitological methods for CVL, particularly in asymptomatic or oligosymptomatic dogs. |
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Exames parasitológicos, imunoistoquímicos e histopatológicos para detecção de Leishmania chagasi em tecidos esplênicos de cães com leishmaniose visceralParasitological, immunohistochemical and histopathological study for Leishmania chagasi detection in spleenic tissues of dogs with visceral leishmaniasisDogsImmunohistochemicalLeishmania (L.) chagasiSpleenVisceral LeishmaniasisThe purpose of this work was a Canine Visceral Leishmaniasis - CVL study by parasitological direct examination of Leishmania (L.) chagasi (imprinting and histological), immunohistochemical test and histopathological analysis using spleen tissues from 34 dogs euthanized by the Zoonotic Disease Control Centre from Ilha Solteira, SP, Brazil. According to the clinical signs, the dogs were divided in three groups: asymptomatics (8 dogs), oligosymptomatics (17 dogs) and symptomatics (9 dogs). After the accomplishment of all diagnostic tests, 22 dogs were considered positives (64.7%) and 12 (35.3%) were negatives to CVL. From these positive dogs, 1/22 (4.5%) was asymptomatic, 12/22 (54.5%) were oligosymptomatics and 8/22 (40.1%) were symptomatics. The histopathological study in spleen tissues from positive, especially symptomatic dogs, showed a diffuse chronic inflammation with thickness of capsular and trabecular regions and there was extense morphologic alteration of the red and white pulp by the presence of abundant macrophages full with amastigotes, the granulomatous inflammatory reaction and haemorragic areas. The data of this work from histopathologic examination and direct microcopic visualization of L. (L.) chagasi showed that the spleen was an useful organ to collect sample tissues for CVL diagnosis. The immunostaining detected the highest number of positive dogs and were considered an important and conclusive method to be used in addition to parasitological methods for CVL, particularly in asymptomatic or oligosymptomatic dogs.Departamento de Biologia e Zootecnia Faculdade de Engenharia - FEIS Universidade Estadual Paulista - UNESPDepartamento de Patologia Veterinária Faculdade de Ciências Agrárias e Veterinárias - FCAV Universidade Estadual Paulista - UNESPCentro de Controle de Zoonoses - CCZ, Ilha Solteira - S PDepartamento de Biologia e Zootecnia Faculdade de Engenharia - FEIS Universidade Estadual Paulista - UNESPDepartamento de Patologia Veterinária Faculdade de Ciências Agrárias e Veterinárias - FCAV Universidade Estadual Paulista - UNESPUniversidade Estadual Paulista (UNESP)Centro de Controle de Zoonoses - CCZTasca, Karen Ingrid [UNESP]Buzetti, Wilma Aparecida Starke [UNESP]Tenorio, Michely da Silva [UNESP]Paulan, Silvana de Cássia [UNESP]Lima, Flávia Luna [UNESP]de Queiroz, Nina Mari Gual Pimenta [UNESP]Machado, Rosângela Zacarias [UNESP]Oliveira, Tricia Maria Ferreira de Souza [UNESP]Neves, Maria Francisca [UNESP]de Noronha Jr., Antonio Carlos Facontide Assis, Juliana [UNESP]2022-04-28T20:57:00Z2022-04-28T20:57:00Z2009-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article27-33Revista Brasileira de Parasitologia Veterinaria, v. 18, n. 1, p. 27-33, 2009.0103-846Xhttp://hdl.handle.net/11449/2256612-s2.0-70350433493Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporRevista Brasileira de Parasitologia Veterinariainfo:eu-repo/semantics/openAccess2022-04-28T20:57:00Zoai:repositorio.unesp.br:11449/225661Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-28T20:57Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Exames parasitológicos, imunoistoquímicos e histopatológicos para detecção de Leishmania chagasi em tecidos esplênicos de cães com leishmaniose visceral Parasitological, immunohistochemical and histopathological study for Leishmania chagasi detection in spleenic tissues of dogs with visceral leishmaniasis |
title |
Exames parasitológicos, imunoistoquímicos e histopatológicos para detecção de Leishmania chagasi em tecidos esplênicos de cães com leishmaniose visceral |
spellingShingle |
Exames parasitológicos, imunoistoquímicos e histopatológicos para detecção de Leishmania chagasi em tecidos esplênicos de cães com leishmaniose visceral Tasca, Karen Ingrid [UNESP] Dogs Immunohistochemical Leishmania (L.) chagasi Spleen Visceral Leishmaniasis |
title_short |
Exames parasitológicos, imunoistoquímicos e histopatológicos para detecção de Leishmania chagasi em tecidos esplênicos de cães com leishmaniose visceral |
title_full |
Exames parasitológicos, imunoistoquímicos e histopatológicos para detecção de Leishmania chagasi em tecidos esplênicos de cães com leishmaniose visceral |
title_fullStr |
Exames parasitológicos, imunoistoquímicos e histopatológicos para detecção de Leishmania chagasi em tecidos esplênicos de cães com leishmaniose visceral |
title_full_unstemmed |
Exames parasitológicos, imunoistoquímicos e histopatológicos para detecção de Leishmania chagasi em tecidos esplênicos de cães com leishmaniose visceral |
title_sort |
Exames parasitológicos, imunoistoquímicos e histopatológicos para detecção de Leishmania chagasi em tecidos esplênicos de cães com leishmaniose visceral |
author |
Tasca, Karen Ingrid [UNESP] |
author_facet |
Tasca, Karen Ingrid [UNESP] Buzetti, Wilma Aparecida Starke [UNESP] Tenorio, Michely da Silva [UNESP] Paulan, Silvana de Cássia [UNESP] Lima, Flávia Luna [UNESP] de Queiroz, Nina Mari Gual Pimenta [UNESP] Machado, Rosângela Zacarias [UNESP] Oliveira, Tricia Maria Ferreira de Souza [UNESP] Neves, Maria Francisca [UNESP] de Noronha Jr., Antonio Carlos Faconti de Assis, Juliana [UNESP] |
author_role |
author |
author2 |
Buzetti, Wilma Aparecida Starke [UNESP] Tenorio, Michely da Silva [UNESP] Paulan, Silvana de Cássia [UNESP] Lima, Flávia Luna [UNESP] de Queiroz, Nina Mari Gual Pimenta [UNESP] Machado, Rosângela Zacarias [UNESP] Oliveira, Tricia Maria Ferreira de Souza [UNESP] Neves, Maria Francisca [UNESP] de Noronha Jr., Antonio Carlos Faconti de Assis, Juliana [UNESP] |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Centro de Controle de Zoonoses - CCZ |
dc.contributor.author.fl_str_mv |
Tasca, Karen Ingrid [UNESP] Buzetti, Wilma Aparecida Starke [UNESP] Tenorio, Michely da Silva [UNESP] Paulan, Silvana de Cássia [UNESP] Lima, Flávia Luna [UNESP] de Queiroz, Nina Mari Gual Pimenta [UNESP] Machado, Rosângela Zacarias [UNESP] Oliveira, Tricia Maria Ferreira de Souza [UNESP] Neves, Maria Francisca [UNESP] de Noronha Jr., Antonio Carlos Faconti de Assis, Juliana [UNESP] |
dc.subject.por.fl_str_mv |
Dogs Immunohistochemical Leishmania (L.) chagasi Spleen Visceral Leishmaniasis |
topic |
Dogs Immunohistochemical Leishmania (L.) chagasi Spleen Visceral Leishmaniasis |
description |
The purpose of this work was a Canine Visceral Leishmaniasis - CVL study by parasitological direct examination of Leishmania (L.) chagasi (imprinting and histological), immunohistochemical test and histopathological analysis using spleen tissues from 34 dogs euthanized by the Zoonotic Disease Control Centre from Ilha Solteira, SP, Brazil. According to the clinical signs, the dogs were divided in three groups: asymptomatics (8 dogs), oligosymptomatics (17 dogs) and symptomatics (9 dogs). After the accomplishment of all diagnostic tests, 22 dogs were considered positives (64.7%) and 12 (35.3%) were negatives to CVL. From these positive dogs, 1/22 (4.5%) was asymptomatic, 12/22 (54.5%) were oligosymptomatics and 8/22 (40.1%) were symptomatics. The histopathological study in spleen tissues from positive, especially symptomatic dogs, showed a diffuse chronic inflammation with thickness of capsular and trabecular regions and there was extense morphologic alteration of the red and white pulp by the presence of abundant macrophages full with amastigotes, the granulomatous inflammatory reaction and haemorragic areas. The data of this work from histopathologic examination and direct microcopic visualization of L. (L.) chagasi showed that the spleen was an useful organ to collect sample tissues for CVL diagnosis. The immunostaining detected the highest number of positive dogs and were considered an important and conclusive method to be used in addition to parasitological methods for CVL, particularly in asymptomatic or oligosymptomatic dogs. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-01-01 2022-04-28T20:57:00Z 2022-04-28T20:57:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Revista Brasileira de Parasitologia Veterinaria, v. 18, n. 1, p. 27-33, 2009. 0103-846X http://hdl.handle.net/11449/225661 2-s2.0-70350433493 |
identifier_str_mv |
Revista Brasileira de Parasitologia Veterinaria, v. 18, n. 1, p. 27-33, 2009. 0103-846X 2-s2.0-70350433493 |
url |
http://hdl.handle.net/11449/225661 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Revista Brasileira de Parasitologia Veterinaria |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
27-33 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1797789845513306112 |