Rescue of susceptibility to second-line drugs in resistant clinical isolates of Mycobacterium tuberculosis

Detalhes bibliográficos
Autor(a) principal: Maringolo-Ribeiro, Camila [UNESP]
Data de Publicação: 2022
Outros Autores: Grecco, Julia A. [UNESP], Bellato, Debora L. [UNESP], Almeida, Aryadne L., Baldin, Vanessa P., Caleffi-Ferracioli, Katiany R., Pavan, Fernando R. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.2217/fmb-2021-0215
http://hdl.handle.net/11449/218993
Resumo: Aim: Antibiotic resistance is one of the biggest threats to global health, and this study aimed better understand how the efflux pumps are related to this process in tuberculosis clinical isolates. Results: The combination of antibiotics plus efflux pumps (EP) inhibitors was able to restore the susceptibility of clinical isolates in 100% of aminoglycosides resistance and 33.3% of the fluoroquinolones resistance. The relative expression of EP genes in pre-extensively drug-resistant isolates showed an increase of up to 1000-times. Conclusion: The rescue of susceptibility in the presence of EP inhibitors, the increased of activity and expression of the EP genes alert that the inhibition of EP can reduce the selection of resistant strains and improve treatment.
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spelling Rescue of susceptibility to second-line drugs in resistant clinical isolates of Mycobacterium tuberculosisaminoglycosideantibiotic resistanceCCCPefflux pumpsfluoroquinolonegene expressionreserpineRT-PCRtuberculosisverapamilAim: Antibiotic resistance is one of the biggest threats to global health, and this study aimed better understand how the efflux pumps are related to this process in tuberculosis clinical isolates. Results: The combination of antibiotics plus efflux pumps (EP) inhibitors was able to restore the susceptibility of clinical isolates in 100% of aminoglycosides resistance and 33.3% of the fluoroquinolones resistance. The relative expression of EP genes in pre-extensively drug-resistant isolates showed an increase of up to 1000-times. Conclusion: The rescue of susceptibility in the presence of EP inhibitors, the increased of activity and expression of the EP genes alert that the inhibition of EP can reduce the selection of resistant strains and improve treatment.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Sao Paulo State UniversityCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, TB Res Lab, Araraquara, SP, BrazilState Univ Maringa UEM, Dept Clin Anal & Biomed, Maringa, Parana, BrazilSao Paulo State Univ UNESP, Sch Pharmaceut Sci, TB Res Lab, Araraquara, SP, BrazilFAPESP: FAPESP 2018/02344-2FAPESP: 2018/12135-1FAPESP: 2020/13497-4CAPES: 001Future Medicine LtdUniversidade Estadual Paulista (UNESP)Universidade Estadual de Maringá (UEM)Maringolo-Ribeiro, Camila [UNESP]Grecco, Julia A. [UNESP]Bellato, Debora L. [UNESP]Almeida, Aryadne L.Baldin, Vanessa P.Caleffi-Ferracioli, Katiany R.Pavan, Fernando R. [UNESP]2022-04-28T18:45:59Z2022-04-28T18:45:59Z2022-03-23info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article511-527http://dx.doi.org/10.2217/fmb-2021-0215Future Microbiology. London: Future Medicine Ltd, v. 17, n. 7, p. 511-527, 2022.1746-0913http://hdl.handle.net/11449/21899310.2217/fmb-2021-0215WOS:000772612000001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFuture Microbiologyinfo:eu-repo/semantics/openAccess2022-04-28T18:45:59Zoai:repositorio.unesp.br:11449/218993Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-28T18:45:59Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Rescue of susceptibility to second-line drugs in resistant clinical isolates of Mycobacterium tuberculosis
title Rescue of susceptibility to second-line drugs in resistant clinical isolates of Mycobacterium tuberculosis
spellingShingle Rescue of susceptibility to second-line drugs in resistant clinical isolates of Mycobacterium tuberculosis
Maringolo-Ribeiro, Camila [UNESP]
aminoglycoside
antibiotic resistance
CCCP
efflux pumps
fluoroquinolone
gene expression
reserpine
RT-PCR
tuberculosis
verapamil
title_short Rescue of susceptibility to second-line drugs in resistant clinical isolates of Mycobacterium tuberculosis
title_full Rescue of susceptibility to second-line drugs in resistant clinical isolates of Mycobacterium tuberculosis
title_fullStr Rescue of susceptibility to second-line drugs in resistant clinical isolates of Mycobacterium tuberculosis
title_full_unstemmed Rescue of susceptibility to second-line drugs in resistant clinical isolates of Mycobacterium tuberculosis
title_sort Rescue of susceptibility to second-line drugs in resistant clinical isolates of Mycobacterium tuberculosis
author Maringolo-Ribeiro, Camila [UNESP]
author_facet Maringolo-Ribeiro, Camila [UNESP]
Grecco, Julia A. [UNESP]
Bellato, Debora L. [UNESP]
Almeida, Aryadne L.
Baldin, Vanessa P.
Caleffi-Ferracioli, Katiany R.
Pavan, Fernando R. [UNESP]
author_role author
author2 Grecco, Julia A. [UNESP]
Bellato, Debora L. [UNESP]
Almeida, Aryadne L.
Baldin, Vanessa P.
Caleffi-Ferracioli, Katiany R.
Pavan, Fernando R. [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Universidade Estadual de Maringá (UEM)
dc.contributor.author.fl_str_mv Maringolo-Ribeiro, Camila [UNESP]
Grecco, Julia A. [UNESP]
Bellato, Debora L. [UNESP]
Almeida, Aryadne L.
Baldin, Vanessa P.
Caleffi-Ferracioli, Katiany R.
Pavan, Fernando R. [UNESP]
dc.subject.por.fl_str_mv aminoglycoside
antibiotic resistance
CCCP
efflux pumps
fluoroquinolone
gene expression
reserpine
RT-PCR
tuberculosis
verapamil
topic aminoglycoside
antibiotic resistance
CCCP
efflux pumps
fluoroquinolone
gene expression
reserpine
RT-PCR
tuberculosis
verapamil
description Aim: Antibiotic resistance is one of the biggest threats to global health, and this study aimed better understand how the efflux pumps are related to this process in tuberculosis clinical isolates. Results: The combination of antibiotics plus efflux pumps (EP) inhibitors was able to restore the susceptibility of clinical isolates in 100% of aminoglycosides resistance and 33.3% of the fluoroquinolones resistance. The relative expression of EP genes in pre-extensively drug-resistant isolates showed an increase of up to 1000-times. Conclusion: The rescue of susceptibility in the presence of EP inhibitors, the increased of activity and expression of the EP genes alert that the inhibition of EP can reduce the selection of resistant strains and improve treatment.
publishDate 2022
dc.date.none.fl_str_mv 2022-04-28T18:45:59Z
2022-04-28T18:45:59Z
2022-03-23
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.2217/fmb-2021-0215
Future Microbiology. London: Future Medicine Ltd, v. 17, n. 7, p. 511-527, 2022.
1746-0913
http://hdl.handle.net/11449/218993
10.2217/fmb-2021-0215
WOS:000772612000001
url http://dx.doi.org/10.2217/fmb-2021-0215
http://hdl.handle.net/11449/218993
identifier_str_mv Future Microbiology. London: Future Medicine Ltd, v. 17, n. 7, p. 511-527, 2022.
1746-0913
10.2217/fmb-2021-0215
WOS:000772612000001
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Future Microbiology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 511-527
dc.publisher.none.fl_str_mv Future Medicine Ltd
publisher.none.fl_str_mv Future Medicine Ltd
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1799964903694401536

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