Gene expression profile in heat-shocked Holstein and Nelore oocytes and cumulus cells
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1071/RD16154 http://hdl.handle.net/11449/175047 |
Resumo: | The present study determined the transcriptome profile in Nelore and Holstein oocytes subjected to heat shock during IVM and the mRNA abundance of selected candidate genes in Nelore and Holstein heat-shocked oocytes and cumulus cells (CC). Holstein and Nelore cows were subjected to in vivo follicle aspiration. Cumulus-oocyte complexes were assigned to control (38.5°C, 22h) or heat shock (41°C for 12h, followed by 38.5°C for 10h) treatment during IVM. Denuded oocytes were subjected to bovine microarray analysis. Transcriptome analysis demonstrated 127, nine and six genes were differentially expressed between breed, temperature and the breed×temperature interaction respectively. Selected differentially expressed genes were evaluated by real-time polymerase chain reaction in oocytes and respective CC. The molecular motor kinesin family member 3A (KIF3A) was upregulated in Holstein oocytes, whereas the pro-apoptotic gene death-associated protein (DAP) and the membrane trafficking gene DENN/MADD domain containing 3 (DENND3) were downregulated in Holstein oocytes. Nelore CC showed increased transcript abundance for tight junction claudin 11 (CLDN11), whereas Holstein CC showed increased transcript abundance for antioxidant metallothionein 1E (MT1E) . Moreover, heat shock downregulated antioxidant MT1E mRNA expression in CC. In conclusion, oocyte transcriptome analysis indicated a strong difference between breeds involving organisation and cell death. In CC, both breed and temperature affected mRNA abundance, involving cellular organisation and oxidative stress. |
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Gene expression profile in heat-shocked Holstein and Nelore oocytes and cumulus cellsBos taurus indicusBos taurus taurusmicroarraymRNAreal-time polymerase chain reaction.The present study determined the transcriptome profile in Nelore and Holstein oocytes subjected to heat shock during IVM and the mRNA abundance of selected candidate genes in Nelore and Holstein heat-shocked oocytes and cumulus cells (CC). Holstein and Nelore cows were subjected to in vivo follicle aspiration. Cumulus-oocyte complexes were assigned to control (38.5°C, 22h) or heat shock (41°C for 12h, followed by 38.5°C for 10h) treatment during IVM. Denuded oocytes were subjected to bovine microarray analysis. Transcriptome analysis demonstrated 127, nine and six genes were differentially expressed between breed, temperature and the breed×temperature interaction respectively. Selected differentially expressed genes were evaluated by real-time polymerase chain reaction in oocytes and respective CC. The molecular motor kinesin family member 3A (KIF3A) was upregulated in Holstein oocytes, whereas the pro-apoptotic gene death-associated protein (DAP) and the membrane trafficking gene DENN/MADD domain containing 3 (DENND3) were downregulated in Holstein oocytes. Nelore CC showed increased transcript abundance for tight junction claudin 11 (CLDN11), whereas Holstein CC showed increased transcript abundance for antioxidant metallothionein 1E (MT1E) . Moreover, heat shock downregulated antioxidant MT1E mRNA expression in CC. In conclusion, oocyte transcriptome analysis indicated a strong difference between breeds involving organisation and cell death. In CC, both breed and temperature affected mRNA abundance, involving cellular organisation and oxidative stress.Department of Pharmacology Institute of Biosciences São Paulo State University (UNESP)Department of Animal Reproduction and Veterinary Radiology School of Veterinary Medicine and Animal Science (FMVZ) São Paulo State University (UNESP), Prof. Dr. Walter Mauricio Correra StreetDepartment of Animal Sciences Luiz de Queiroz College of Agriculture (ESALQ) University of São Paulo (USP), Pádua Dias Avenue 11Department of Animal Reproduction School of Veterinary Medicine and Animal Science (FMVZ) University of São Paulo (USP), Orlando Marques de Paiva Street 87Institute of Biosciences Chemistry and Pharmacology Federal University of Saõ Paulo (UNIFESP), Prof. Artur Riedel Street 275Department of Pharmacology Institute of Biosciences São Paulo State University (UNESP)Department of Animal Reproduction and Veterinary Radiology School of Veterinary Medicine and Animal Science (FMVZ) São Paulo State University (UNESP), Prof. Dr. Walter Mauricio Correra StreetUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Universidade Federal de São Paulo (UNIFESP)Ticianelli, J. S. [UNESP]Emanuelli, I. P. [UNESP]Satrapa, R. A. [UNESP]Castilho, A. C.S. [UNESP]Loureiro, B. [UNESP]Sudano, M. J. [UNESP]Fontes, P. K. [UNESP]Pinto, R. F.P. [UNESP]Razza, E. M. [UNESP]Surjus, R. S.Sartori, R.Assumpção, M. E.O.A.Visintin, J. A.Barros, C. M. [UNESP]Paula-Lopes, F. F. [UNESP]2018-12-11T17:14:00Z2018-12-11T17:14:00Z2017-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1787-1802application/pdfhttp://dx.doi.org/10.1071/RD16154Reproduction, Fertility and Development, v. 29, n. 9, p. 1787-1802, 2017.1448-59901031-3613http://hdl.handle.net/11449/17504710.1071/RD161542-s2.0-850274934502-s2.0-85027493450.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengReproduction, Fertility and Development0,681info:eu-repo/semantics/openAccess2023-11-04T06:11:52Zoai:repositorio.unesp.br:11449/175047Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-04T06:11:52Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Gene expression profile in heat-shocked Holstein and Nelore oocytes and cumulus cells |
title |
Gene expression profile in heat-shocked Holstein and Nelore oocytes and cumulus cells |
spellingShingle |
Gene expression profile in heat-shocked Holstein and Nelore oocytes and cumulus cells Ticianelli, J. S. [UNESP] Bos taurus indicus Bos taurus taurus microarray mRNA real-time polymerase chain reaction. |
title_short |
Gene expression profile in heat-shocked Holstein and Nelore oocytes and cumulus cells |
title_full |
Gene expression profile in heat-shocked Holstein and Nelore oocytes and cumulus cells |
title_fullStr |
Gene expression profile in heat-shocked Holstein and Nelore oocytes and cumulus cells |
title_full_unstemmed |
Gene expression profile in heat-shocked Holstein and Nelore oocytes and cumulus cells |
title_sort |
Gene expression profile in heat-shocked Holstein and Nelore oocytes and cumulus cells |
author |
Ticianelli, J. S. [UNESP] |
author_facet |
Ticianelli, J. S. [UNESP] Emanuelli, I. P. [UNESP] Satrapa, R. A. [UNESP] Castilho, A. C.S. [UNESP] Loureiro, B. [UNESP] Sudano, M. J. [UNESP] Fontes, P. K. [UNESP] Pinto, R. F.P. [UNESP] Razza, E. M. [UNESP] Surjus, R. S. Sartori, R. Assumpção, M. E.O.A. Visintin, J. A. Barros, C. M. [UNESP] Paula-Lopes, F. F. [UNESP] |
author_role |
author |
author2 |
Emanuelli, I. P. [UNESP] Satrapa, R. A. [UNESP] Castilho, A. C.S. [UNESP] Loureiro, B. [UNESP] Sudano, M. J. [UNESP] Fontes, P. K. [UNESP] Pinto, R. F.P. [UNESP] Razza, E. M. [UNESP] Surjus, R. S. Sartori, R. Assumpção, M. E.O.A. Visintin, J. A. Barros, C. M. [UNESP] Paula-Lopes, F. F. [UNESP] |
author2_role |
author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Ticianelli, J. S. [UNESP] Emanuelli, I. P. [UNESP] Satrapa, R. A. [UNESP] Castilho, A. C.S. [UNESP] Loureiro, B. [UNESP] Sudano, M. J. [UNESP] Fontes, P. K. [UNESP] Pinto, R. F.P. [UNESP] Razza, E. M. [UNESP] Surjus, R. S. Sartori, R. Assumpção, M. E.O.A. Visintin, J. A. Barros, C. M. [UNESP] Paula-Lopes, F. F. [UNESP] |
dc.subject.por.fl_str_mv |
Bos taurus indicus Bos taurus taurus microarray mRNA real-time polymerase chain reaction. |
topic |
Bos taurus indicus Bos taurus taurus microarray mRNA real-time polymerase chain reaction. |
description |
The present study determined the transcriptome profile in Nelore and Holstein oocytes subjected to heat shock during IVM and the mRNA abundance of selected candidate genes in Nelore and Holstein heat-shocked oocytes and cumulus cells (CC). Holstein and Nelore cows were subjected to in vivo follicle aspiration. Cumulus-oocyte complexes were assigned to control (38.5°C, 22h) or heat shock (41°C for 12h, followed by 38.5°C for 10h) treatment during IVM. Denuded oocytes were subjected to bovine microarray analysis. Transcriptome analysis demonstrated 127, nine and six genes were differentially expressed between breed, temperature and the breed×temperature interaction respectively. Selected differentially expressed genes were evaluated by real-time polymerase chain reaction in oocytes and respective CC. The molecular motor kinesin family member 3A (KIF3A) was upregulated in Holstein oocytes, whereas the pro-apoptotic gene death-associated protein (DAP) and the membrane trafficking gene DENN/MADD domain containing 3 (DENND3) were downregulated in Holstein oocytes. Nelore CC showed increased transcript abundance for tight junction claudin 11 (CLDN11), whereas Holstein CC showed increased transcript abundance for antioxidant metallothionein 1E (MT1E) . Moreover, heat shock downregulated antioxidant MT1E mRNA expression in CC. In conclusion, oocyte transcriptome analysis indicated a strong difference between breeds involving organisation and cell death. In CC, both breed and temperature affected mRNA abundance, involving cellular organisation and oxidative stress. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-01-01 2018-12-11T17:14:00Z 2018-12-11T17:14:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1071/RD16154 Reproduction, Fertility and Development, v. 29, n. 9, p. 1787-1802, 2017. 1448-5990 1031-3613 http://hdl.handle.net/11449/175047 10.1071/RD16154 2-s2.0-85027493450 2-s2.0-85027493450.pdf |
url |
http://dx.doi.org/10.1071/RD16154 http://hdl.handle.net/11449/175047 |
identifier_str_mv |
Reproduction, Fertility and Development, v. 29, n. 9, p. 1787-1802, 2017. 1448-5990 1031-3613 10.1071/RD16154 2-s2.0-85027493450 2-s2.0-85027493450.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Reproduction, Fertility and Development 0,681 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1787-1802 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797789609271230464 |