Semi-quantitative analysis of morphological changes in bee tissues: A toxicological approach
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.chemosphere.2019.06.225 http://hdl.handle.net/11449/196256 |
Resumo: | During foraging, bees are exposed to sublethal doses of insecticides, which can cause morphological changes to various organs, such as the midgut, Malpighian tubules, and mushroon body. Thus, the purpose of this study was to establish a scoring system to evaluate these alterations based on the damage caused and its reversibility. Therefore, a grade 1 score indicates a minimal and easily reversible lesion, increased apocrine secretion, increased cell elimination into the lumen, and a larger quantity of spherocrystals; grade 2 was assigned to moderate and typically reversible injuries, such as changes in the brush border, vacuolation/loss of cytoplasmic material, presence/height of the brush border, and cell swelling; and grade 3 was assigned to serious and irreversible, loss of cell nests of regenerative cells, pyknosis, and loss of contact between Kenyon cells. In addition, frequency values were assigned since the alterations can occur at different frequencies according to the insecticide and the bees exposed; the frequency ranges from 0 to 6, with 0 representing the absence of an alteration and 6 representing a high frequency occurrence. Based on the analyses, we conclude that each change causes morphological damage, which may or may not be irreversible and could affect the health of the colony. (C) 2019 Elsevier Ltd. All rights reserved. |
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Semi-quantitative analysis of morphological changes in bee tissues: A toxicological approachMidgutMalpighian tubulesMushroom bodyScoreInsecticideDuring foraging, bees are exposed to sublethal doses of insecticides, which can cause morphological changes to various organs, such as the midgut, Malpighian tubules, and mushroon body. Thus, the purpose of this study was to establish a scoring system to evaluate these alterations based on the damage caused and its reversibility. Therefore, a grade 1 score indicates a minimal and easily reversible lesion, increased apocrine secretion, increased cell elimination into the lumen, and a larger quantity of spherocrystals; grade 2 was assigned to moderate and typically reversible injuries, such as changes in the brush border, vacuolation/loss of cytoplasmic material, presence/height of the brush border, and cell swelling; and grade 3 was assigned to serious and irreversible, loss of cell nests of regenerative cells, pyknosis, and loss of contact between Kenyon cells. In addition, frequency values were assigned since the alterations can occur at different frequencies according to the insecticide and the bees exposed; the frequency ranges from 0 to 6, with 0 representing the absence of an alteration and 6 representing a high frequency occurrence. Based on the analyses, we conclude that each change causes morphological damage, which may or may not be irreversible and could affect the health of the colony. (C) 2019 Elsevier Ltd. All rights reserved.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Paulista, Dept Biol, Ctr Estudos Insetos Socials, Ave 24-A,1-515, BR-13506900 Rio Claro, SP, BrazilUniv Fed Sao Carlos, Ctr Ciencias Agr, Dept Ciencias Nat Matemat & Educ, Campus Araras,Rodovia Anhanguera SP-330,Km 174, BR-13600970 Araras, SP, BrazilUniv Fed Sao Carlos, Dept Biol, Ctr Ciencias & Tecnol Sustentabilidade, Campus Sorocaba,Rodovia Joao Leme dos Santos, BR-18052780 Sorocaba, SP, BrazilUniv Estadual Paulista, Dept Biol, Ctr Estudos Insetos Socials, Ave 24-A,1-515, BR-13506900 Rio Claro, SP, BrazilCAPES: 001FAPESP: 2014/12488-0FAPESP: 2013/09555-5FAPESP: 2017/21097-3Elsevier B.V.Universidade Estadual Paulista (Unesp)Universidade Federal de São Carlos (UFSCar)Grella, Tatiane Caroline [UNESP]Soares-Lima, Hellen MariaMalaspina, Osmar [UNESP]Ferreira Nocelli, Roberta Cornelio2020-12-10T19:38:45Z2020-12-10T19:38:45Z2019-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article5http://dx.doi.org/10.1016/j.chemosphere.2019.06.225Chemosphere. Oxford: Pergamon-elsevier Science Ltd, v. 236, 5 p., 2019.0045-6535http://hdl.handle.net/11449/19625610.1016/j.chemosphere.2019.06.225WOS:00049163450005175385560855058190000-0002-1650-257XWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengChemosphereinfo:eu-repo/semantics/openAccess2021-10-23T05:55:16Zoai:repositorio.unesp.br:11449/196256Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T05:55:16Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Semi-quantitative analysis of morphological changes in bee tissues: A toxicological approach |
title |
Semi-quantitative analysis of morphological changes in bee tissues: A toxicological approach |
spellingShingle |
Semi-quantitative analysis of morphological changes in bee tissues: A toxicological approach Grella, Tatiane Caroline [UNESP] Midgut Malpighian tubules Mushroom body Score Insecticide |
title_short |
Semi-quantitative analysis of morphological changes in bee tissues: A toxicological approach |
title_full |
Semi-quantitative analysis of morphological changes in bee tissues: A toxicological approach |
title_fullStr |
Semi-quantitative analysis of morphological changes in bee tissues: A toxicological approach |
title_full_unstemmed |
Semi-quantitative analysis of morphological changes in bee tissues: A toxicological approach |
title_sort |
Semi-quantitative analysis of morphological changes in bee tissues: A toxicological approach |
author |
Grella, Tatiane Caroline [UNESP] |
author_facet |
Grella, Tatiane Caroline [UNESP] Soares-Lima, Hellen Maria Malaspina, Osmar [UNESP] Ferreira Nocelli, Roberta Cornelio |
author_role |
author |
author2 |
Soares-Lima, Hellen Maria Malaspina, Osmar [UNESP] Ferreira Nocelli, Roberta Cornelio |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal de São Carlos (UFSCar) |
dc.contributor.author.fl_str_mv |
Grella, Tatiane Caroline [UNESP] Soares-Lima, Hellen Maria Malaspina, Osmar [UNESP] Ferreira Nocelli, Roberta Cornelio |
dc.subject.por.fl_str_mv |
Midgut Malpighian tubules Mushroom body Score Insecticide |
topic |
Midgut Malpighian tubules Mushroom body Score Insecticide |
description |
During foraging, bees are exposed to sublethal doses of insecticides, which can cause morphological changes to various organs, such as the midgut, Malpighian tubules, and mushroon body. Thus, the purpose of this study was to establish a scoring system to evaluate these alterations based on the damage caused and its reversibility. Therefore, a grade 1 score indicates a minimal and easily reversible lesion, increased apocrine secretion, increased cell elimination into the lumen, and a larger quantity of spherocrystals; grade 2 was assigned to moderate and typically reversible injuries, such as changes in the brush border, vacuolation/loss of cytoplasmic material, presence/height of the brush border, and cell swelling; and grade 3 was assigned to serious and irreversible, loss of cell nests of regenerative cells, pyknosis, and loss of contact between Kenyon cells. In addition, frequency values were assigned since the alterations can occur at different frequencies according to the insecticide and the bees exposed; the frequency ranges from 0 to 6, with 0 representing the absence of an alteration and 6 representing a high frequency occurrence. Based on the analyses, we conclude that each change causes morphological damage, which may or may not be irreversible and could affect the health of the colony. (C) 2019 Elsevier Ltd. All rights reserved. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-12-01 2020-12-10T19:38:45Z 2020-12-10T19:38:45Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.chemosphere.2019.06.225 Chemosphere. Oxford: Pergamon-elsevier Science Ltd, v. 236, 5 p., 2019. 0045-6535 http://hdl.handle.net/11449/196256 10.1016/j.chemosphere.2019.06.225 WOS:000491634500051 7538556085505819 0000-0002-1650-257X |
url |
http://dx.doi.org/10.1016/j.chemosphere.2019.06.225 http://hdl.handle.net/11449/196256 |
identifier_str_mv |
Chemosphere. Oxford: Pergamon-elsevier Science Ltd, v. 236, 5 p., 2019. 0045-6535 10.1016/j.chemosphere.2019.06.225 WOS:000491634500051 7538556085505819 0000-0002-1650-257X |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Chemosphere |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
5 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965177880248320 |