Immunomodulatory effects and anti-Candida activity of lactobacilli in macrophages and in invertebrate model of Galleria mellonella
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.micpath.2017.08.006 http://hdl.handle.net/11449/175075 |
Resumo: | Due to the growing number of multi-resistant Candida spp., adjuvant treatments that may help combat these fungal pathogens are relevant and useful. This study evaluated the immunomodulation and anti-Candida activity of Lactobacillus rhamnosus (LR), Lactobacillus acidophilus and Lactobacillus paracasei suspensions, either single- or multiple-strain, in mouse macrophages (RAW 264.7) and Galleria mellonella (GM). Mouse macrophages were activated by different lactobacilli suspensions and challenged with C. albicans (CA). Tumor necrosis factor (TNF)-α, interleukin IL-1β, IL-6 and IL-17 production and cell viability were investigated. LR was the best suspension for stimulating all evaluated cytokines and thus was used in subsequent in vivo assays. Two C. albicans clinical strains, CA21 and CA60, were then added to the GM assays to further confirm the results. LR suspension was injected into the larvae 24 h before challenging with CA. Survival curve, CFU per larva and hemocytes were counted. In the GM, the LR suspension increased the survival rate and hemocyte counts and decreased the CFU per larva counts for all groups. Lactobacilli suspensions presented strain-dependent immunomodulation; however, single suspensions showed better results. Anti-Candida activity was demonstrated by decreased Candida counts in the GM with the use of LR. |
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Immunomodulatory effects and anti-Candida activity of lactobacilli in macrophages and in invertebrate model of Galleria mellonellaLactic acid bacteriaLactobacillusMacrophagesMycologyProbioticYeastsDue to the growing number of multi-resistant Candida spp., adjuvant treatments that may help combat these fungal pathogens are relevant and useful. This study evaluated the immunomodulation and anti-Candida activity of Lactobacillus rhamnosus (LR), Lactobacillus acidophilus and Lactobacillus paracasei suspensions, either single- or multiple-strain, in mouse macrophages (RAW 264.7) and Galleria mellonella (GM). Mouse macrophages were activated by different lactobacilli suspensions and challenged with C. albicans (CA). Tumor necrosis factor (TNF)-α, interleukin IL-1β, IL-6 and IL-17 production and cell viability were investigated. LR was the best suspension for stimulating all evaluated cytokines and thus was used in subsequent in vivo assays. Two C. albicans clinical strains, CA21 and CA60, were then added to the GM assays to further confirm the results. LR suspension was injected into the larvae 24 h before challenging with CA. Survival curve, CFU per larva and hemocytes were counted. In the GM, the LR suspension increased the survival rate and hemocyte counts and decreased the CFU per larva counts for all groups. Lactobacilli suspensions presented strain-dependent immunomodulation; however, single suspensions showed better results. Anti-Candida activity was demonstrated by decreased Candida counts in the GM with the use of LR.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Biosciences and Oral Diagnosis São Paulo State University (Unesp) Institute of Science and Technology, São José dos Campos, Avenida Engenheiro Francisco José Longo 777, São DimasInstitute of Basic Biosciences School of Medicine University of Taubaté, Av. Tiradentes, 500, CentroDepartment of Biosciences and Oral Diagnosis São Paulo State University (Unesp) Institute of Science and Technology, São José dos Campos, Avenida Engenheiro Francisco José Longo 777, São DimasFAPESP: 2016/17166-7Universidade Estadual Paulista (Unesp)University of Taubatéde Oliveira, Felipe Eduardo [UNESP]Rossoni, Rodnei Dennis [UNESP]de Barros, Patricia Pimentel [UNESP]Begnini, Barbara Evelyn [UNESP]Junqueira, Juliana Campos [UNESP]Jorge, Antonio Olavo Cardoso [UNESP]Leão, Mariella Vieira Pereirade Oliveira, Luciane Dias [UNESP]2018-12-11T17:14:06Z2018-12-11T17:14:06Z2017-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article603-611application/pdfhttp://dx.doi.org/10.1016/j.micpath.2017.08.006Microbial Pathogenesis, v. 110, p. 603-611.1096-12080882-4010http://hdl.handle.net/11449/17507510.1016/j.micpath.2017.08.0062-s2.0-850280042602-s2.0-85028004260.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMicrobial Pathogenesis0,7510,751info:eu-repo/semantics/openAccess2023-12-15T06:18:50Zoai:repositorio.unesp.br:11449/175075Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-15T06:18:50Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Immunomodulatory effects and anti-Candida activity of lactobacilli in macrophages and in invertebrate model of Galleria mellonella |
title |
Immunomodulatory effects and anti-Candida activity of lactobacilli in macrophages and in invertebrate model of Galleria mellonella |
spellingShingle |
Immunomodulatory effects and anti-Candida activity of lactobacilli in macrophages and in invertebrate model of Galleria mellonella de Oliveira, Felipe Eduardo [UNESP] Lactic acid bacteria Lactobacillus Macrophages Mycology Probiotic Yeasts |
title_short |
Immunomodulatory effects and anti-Candida activity of lactobacilli in macrophages and in invertebrate model of Galleria mellonella |
title_full |
Immunomodulatory effects and anti-Candida activity of lactobacilli in macrophages and in invertebrate model of Galleria mellonella |
title_fullStr |
Immunomodulatory effects and anti-Candida activity of lactobacilli in macrophages and in invertebrate model of Galleria mellonella |
title_full_unstemmed |
Immunomodulatory effects and anti-Candida activity of lactobacilli in macrophages and in invertebrate model of Galleria mellonella |
title_sort |
Immunomodulatory effects and anti-Candida activity of lactobacilli in macrophages and in invertebrate model of Galleria mellonella |
author |
de Oliveira, Felipe Eduardo [UNESP] |
author_facet |
de Oliveira, Felipe Eduardo [UNESP] Rossoni, Rodnei Dennis [UNESP] de Barros, Patricia Pimentel [UNESP] Begnini, Barbara Evelyn [UNESP] Junqueira, Juliana Campos [UNESP] Jorge, Antonio Olavo Cardoso [UNESP] Leão, Mariella Vieira Pereira de Oliveira, Luciane Dias [UNESP] |
author_role |
author |
author2 |
Rossoni, Rodnei Dennis [UNESP] de Barros, Patricia Pimentel [UNESP] Begnini, Barbara Evelyn [UNESP] Junqueira, Juliana Campos [UNESP] Jorge, Antonio Olavo Cardoso [UNESP] Leão, Mariella Vieira Pereira de Oliveira, Luciane Dias [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) University of Taubaté |
dc.contributor.author.fl_str_mv |
de Oliveira, Felipe Eduardo [UNESP] Rossoni, Rodnei Dennis [UNESP] de Barros, Patricia Pimentel [UNESP] Begnini, Barbara Evelyn [UNESP] Junqueira, Juliana Campos [UNESP] Jorge, Antonio Olavo Cardoso [UNESP] Leão, Mariella Vieira Pereira de Oliveira, Luciane Dias [UNESP] |
dc.subject.por.fl_str_mv |
Lactic acid bacteria Lactobacillus Macrophages Mycology Probiotic Yeasts |
topic |
Lactic acid bacteria Lactobacillus Macrophages Mycology Probiotic Yeasts |
description |
Due to the growing number of multi-resistant Candida spp., adjuvant treatments that may help combat these fungal pathogens are relevant and useful. This study evaluated the immunomodulation and anti-Candida activity of Lactobacillus rhamnosus (LR), Lactobacillus acidophilus and Lactobacillus paracasei suspensions, either single- or multiple-strain, in mouse macrophages (RAW 264.7) and Galleria mellonella (GM). Mouse macrophages were activated by different lactobacilli suspensions and challenged with C. albicans (CA). Tumor necrosis factor (TNF)-α, interleukin IL-1β, IL-6 and IL-17 production and cell viability were investigated. LR was the best suspension for stimulating all evaluated cytokines and thus was used in subsequent in vivo assays. Two C. albicans clinical strains, CA21 and CA60, were then added to the GM assays to further confirm the results. LR suspension was injected into the larvae 24 h before challenging with CA. Survival curve, CFU per larva and hemocytes were counted. In the GM, the LR suspension increased the survival rate and hemocyte counts and decreased the CFU per larva counts for all groups. Lactobacilli suspensions presented strain-dependent immunomodulation; however, single suspensions showed better results. Anti-Candida activity was demonstrated by decreased Candida counts in the GM with the use of LR. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-09-01 2018-12-11T17:14:06Z 2018-12-11T17:14:06Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.micpath.2017.08.006 Microbial Pathogenesis, v. 110, p. 603-611. 1096-1208 0882-4010 http://hdl.handle.net/11449/175075 10.1016/j.micpath.2017.08.006 2-s2.0-85028004260 2-s2.0-85028004260.pdf |
url |
http://dx.doi.org/10.1016/j.micpath.2017.08.006 http://hdl.handle.net/11449/175075 |
identifier_str_mv |
Microbial Pathogenesis, v. 110, p. 603-611. 1096-1208 0882-4010 10.1016/j.micpath.2017.08.006 2-s2.0-85028004260 2-s2.0-85028004260.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Microbial Pathogenesis 0,751 0,751 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
603-611 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965295668887552 |