Leptospirosis diagnosis among patients suspected of dengue fever in Brazil
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/1678-9199-JVATITD-2020-0118 http://hdl.handle.net/11449/210179 |
Resumo: | Background: The early symptoms of leptospirosis and dengue fever are difficult to distinguish and can cause diagnostic confusion. Due to the large dengue epidemics that has occurred in Brazil in recent years, it is possible that cases of leptospirosis were unreported. Therefore, we performed a retrospective study to detect leptospirosis in patients who were tested for dengue, but whose laboratory diagnoses were negative. Methods: Sera samples from 2,017 patients from 48 cities located in the central region of Sao Paulo state, Brazil, were studied. All samples were subjected to the microscopic agglutination test (MAT), 305 of which were taken from patients five days or less since the onset of symptoms, and were additionally subjected to real-time polymerase chain reaction (PCR). Results: The overall prevalence of leptospirosis cases was 21 (1.04%), with 20 through MAT (18 for Icterohaemorrhagiae and two for the Cynopteri serogroup) and one through PCR (amplicon sequencing compatible with Leptospira interrogans). According to previously established criteria, eight cases of leptospirosis were classified as confirmed and 13 as probable. The Brazilian notification system for health surveillance had no records for 16 patients positive for leptospirosis and, thus, they were considered unreported cases. Statistical analyses revealed that the prevalence of leptospirosis was higher in men (1.56%) than in women (0.56%), and the mean age was higher in positive patients (43.7 years) than in negative ones (32.3 years). Conclusion: The results indicated that patients suspected of dengue fever had evidence of leptospirosis or Leptospira infection, and most of these cases were unreported in the Brazilian notification system. The high burden of dengue may contribute to the misdiagnosis of leptospirosis, and health professionals should increase their awareness of leptospirosis as an important differential diagnosis of patients with suspicion of dengue. |
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Leptospirosis diagnosis among patients suspected of dengue fever in BrazilLeptospiraMicroscopic agglutination testPCRUnreported diseaseZoonosisBackground: The early symptoms of leptospirosis and dengue fever are difficult to distinguish and can cause diagnostic confusion. Due to the large dengue epidemics that has occurred in Brazil in recent years, it is possible that cases of leptospirosis were unreported. Therefore, we performed a retrospective study to detect leptospirosis in patients who were tested for dengue, but whose laboratory diagnoses were negative. Methods: Sera samples from 2,017 patients from 48 cities located in the central region of Sao Paulo state, Brazil, were studied. All samples were subjected to the microscopic agglutination test (MAT), 305 of which were taken from patients five days or less since the onset of symptoms, and were additionally subjected to real-time polymerase chain reaction (PCR). Results: The overall prevalence of leptospirosis cases was 21 (1.04%), with 20 through MAT (18 for Icterohaemorrhagiae and two for the Cynopteri serogroup) and one through PCR (amplicon sequencing compatible with Leptospira interrogans). According to previously established criteria, eight cases of leptospirosis were classified as confirmed and 13 as probable. The Brazilian notification system for health surveillance had no records for 16 patients positive for leptospirosis and, thus, they were considered unreported cases. Statistical analyses revealed that the prevalence of leptospirosis was higher in men (1.56%) than in women (0.56%), and the mean age was higher in positive patients (43.7 years) than in negative ones (32.3 years). Conclusion: The results indicated that patients suspected of dengue fever had evidence of leptospirosis or Leptospira infection, and most of these cases were unreported in the Brazilian notification system. The high burden of dengue may contribute to the misdiagnosis of leptospirosis, and health professionals should increase their awareness of leptospirosis as an important differential diagnosis of patients with suspicion of dengue.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Sao Paulo State Univ UNESP, Sch Vet Med & Anim Husb, Dept Anim Prod & Prevent Vet Med, Botucatu, SP, BrazilAdolfo Lutz Inst, Ctr Reg Lab Bauru 2, Bauru, SP, BrazilButantan Inst, Lab Appl Toxinol, Sao Paulo, SP, BrazilSao Paulo State Univ UNESP, Sch Vet Med & Anim Husb, Dept Anim Prod & Prevent Vet Med, Botucatu, SP, BrazilFAPESP: 2015/06446-6Cevap-sao Paulo State Univ-unespUniversidade Estadual Paulista (Unesp)Adolfo Lutz InstButantan InstFornazari, Felipe [UNESP]Richini-Pereira, Virginia BodelaoJoaquim, Samea Fernandes [UNESP]Nachtigall, Pedro GabrielLangoni, Helio [UNESP]2021-06-25T12:42:01Z2021-06-25T12:42:01Z2021-03-26info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article7application/pdfhttp://dx.doi.org/10.1590/1678-9199-JVATITD-2020-0118Journal Of Venomous Animals And Toxins Including Tropical Diseases. Botucatu: Cevap-sao Paulo State Univ-unesp, v. 27, 7 p., 2021.http://hdl.handle.net/11449/21017910.1590/1678-9199-JVATITD-2020-0118S1678-91992021000100502WOS:000635447100001S1678-91992021000100502.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal Of Venomous Animals And Toxins Including Tropical Diseasesinfo:eu-repo/semantics/openAccess2024-01-15T06:22:55Zoai:repositorio.unesp.br:11449/210179Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-15T06:22:55Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Leptospirosis diagnosis among patients suspected of dengue fever in Brazil |
title |
Leptospirosis diagnosis among patients suspected of dengue fever in Brazil |
spellingShingle |
Leptospirosis diagnosis among patients suspected of dengue fever in Brazil Fornazari, Felipe [UNESP] Leptospira Microscopic agglutination test PCR Unreported disease Zoonosis |
title_short |
Leptospirosis diagnosis among patients suspected of dengue fever in Brazil |
title_full |
Leptospirosis diagnosis among patients suspected of dengue fever in Brazil |
title_fullStr |
Leptospirosis diagnosis among patients suspected of dengue fever in Brazil |
title_full_unstemmed |
Leptospirosis diagnosis among patients suspected of dengue fever in Brazil |
title_sort |
Leptospirosis diagnosis among patients suspected of dengue fever in Brazil |
author |
Fornazari, Felipe [UNESP] |
author_facet |
Fornazari, Felipe [UNESP] Richini-Pereira, Virginia Bodelao Joaquim, Samea Fernandes [UNESP] Nachtigall, Pedro Gabriel Langoni, Helio [UNESP] |
author_role |
author |
author2 |
Richini-Pereira, Virginia Bodelao Joaquim, Samea Fernandes [UNESP] Nachtigall, Pedro Gabriel Langoni, Helio [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Adolfo Lutz Inst Butantan Inst |
dc.contributor.author.fl_str_mv |
Fornazari, Felipe [UNESP] Richini-Pereira, Virginia Bodelao Joaquim, Samea Fernandes [UNESP] Nachtigall, Pedro Gabriel Langoni, Helio [UNESP] |
dc.subject.por.fl_str_mv |
Leptospira Microscopic agglutination test PCR Unreported disease Zoonosis |
topic |
Leptospira Microscopic agglutination test PCR Unreported disease Zoonosis |
description |
Background: The early symptoms of leptospirosis and dengue fever are difficult to distinguish and can cause diagnostic confusion. Due to the large dengue epidemics that has occurred in Brazil in recent years, it is possible that cases of leptospirosis were unreported. Therefore, we performed a retrospective study to detect leptospirosis in patients who were tested for dengue, but whose laboratory diagnoses were negative. Methods: Sera samples from 2,017 patients from 48 cities located in the central region of Sao Paulo state, Brazil, were studied. All samples were subjected to the microscopic agglutination test (MAT), 305 of which were taken from patients five days or less since the onset of symptoms, and were additionally subjected to real-time polymerase chain reaction (PCR). Results: The overall prevalence of leptospirosis cases was 21 (1.04%), with 20 through MAT (18 for Icterohaemorrhagiae and two for the Cynopteri serogroup) and one through PCR (amplicon sequencing compatible with Leptospira interrogans). According to previously established criteria, eight cases of leptospirosis were classified as confirmed and 13 as probable. The Brazilian notification system for health surveillance had no records for 16 patients positive for leptospirosis and, thus, they were considered unreported cases. Statistical analyses revealed that the prevalence of leptospirosis was higher in men (1.56%) than in women (0.56%), and the mean age was higher in positive patients (43.7 years) than in negative ones (32.3 years). Conclusion: The results indicated that patients suspected of dengue fever had evidence of leptospirosis or Leptospira infection, and most of these cases were unreported in the Brazilian notification system. The high burden of dengue may contribute to the misdiagnosis of leptospirosis, and health professionals should increase their awareness of leptospirosis as an important differential diagnosis of patients with suspicion of dengue. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T12:42:01Z 2021-06-25T12:42:01Z 2021-03-26 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1678-9199-JVATITD-2020-0118 Journal Of Venomous Animals And Toxins Including Tropical Diseases. Botucatu: Cevap-sao Paulo State Univ-unesp, v. 27, 7 p., 2021. http://hdl.handle.net/11449/210179 10.1590/1678-9199-JVATITD-2020-0118 S1678-91992021000100502 WOS:000635447100001 S1678-91992021000100502.pdf |
url |
http://dx.doi.org/10.1590/1678-9199-JVATITD-2020-0118 http://hdl.handle.net/11449/210179 |
identifier_str_mv |
Journal Of Venomous Animals And Toxins Including Tropical Diseases. Botucatu: Cevap-sao Paulo State Univ-unesp, v. 27, 7 p., 2021. 10.1590/1678-9199-JVATITD-2020-0118 S1678-91992021000100502 WOS:000635447100001 S1678-91992021000100502.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal Of Venomous Animals And Toxins Including Tropical Diseases |
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info:eu-repo/semantics/openAccess |
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openAccess |
dc.format.none.fl_str_mv |
7 application/pdf |
dc.publisher.none.fl_str_mv |
Cevap-sao Paulo State Univ-unesp |
publisher.none.fl_str_mv |
Cevap-sao Paulo State Univ-unesp |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
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UNESP |
reponame_str |
Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
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