Vegetative propagation of Mikania glomerata: Micro-propagation and cuttings
Autor(a) principal: | |
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Data de Publicação: | 1999 |
Outros Autores: | , |
Tipo de documento: | Artigo de conferência |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.actahort.org/books/502/502_57.htm http://hdl.handle.net/11449/66000 |
Resumo: | The scope of this work was to compare two systems for vegetative propagation: conventional one (from cut stems) and in vitro micropropagation from axillary buds. Nodal segments (1 cm) of Mikania glomerata were used as explants. The experiments were evaluated in relation to number of shoots; % of rooting; number of roots and total fresh weight. Multiple shoots developed in MS containing 0.5 mg/L BAP. Rooting was induced in the presence of 1.0 mg/L IBA. Stems with five buds and one pair of leaves were the most appropriate for the production of cuttings. The time necessary for developing a protocol for the production of M. glomerata micropropagated plantlets was 6 months, whereas only half time was required to produce plantlets from stem cuttings. The greatest problem met during micropropagation was the culture contamination by endophytic bacteria and fungi. |
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Repositório Institucional da UNESP |
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spelling |
Vegetative propagation of Mikania glomerata: Micro-propagation and cuttingsGuacoMikania glomerataStalksTissue cultureThe scope of this work was to compare two systems for vegetative propagation: conventional one (from cut stems) and in vitro micropropagation from axillary buds. Nodal segments (1 cm) of Mikania glomerata were used as explants. The experiments were evaluated in relation to number of shoots; % of rooting; number of roots and total fresh weight. Multiple shoots developed in MS containing 0.5 mg/L BAP. Rooting was induced in the presence of 1.0 mg/L IBA. Stems with five buds and one pair of leaves were the most appropriate for the production of cuttings. The time necessary for developing a protocol for the production of M. glomerata micropropagated plantlets was 6 months, whereas only half time was required to produce plantlets from stem cuttings. The greatest problem met during micropropagation was the culture contamination by endophytic bacteria and fungi.Departamento de Biotecnologia Vegetal Universidade de Ribeirão Preto UNAERP, 14096380 Ribeirão Preto, SPDepartamento de Horticultura Faculdade de Agronomia UNESP, Botucatu, SPDepartamento de Horticultura Faculdade de Agronomia UNESP, Botucatu, SPUNAERPUniversidade Estadual Paulista (Unesp)Pereira, Ana Maria Soares [UNESP]França, S. C.Câmara, Francisco Luiz Araújo [UNESP]2014-05-27T11:19:50Z2014-05-27T11:19:50Z1999-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObject347-352http://www.actahort.org/books/502/502_57.htmActa Horticulturae, v. 502, p. 347-352.0567-7572http://hdl.handle.net/11449/660002-s2.0-84879453141Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengActa Horticulturae0,198info:eu-repo/semantics/openAccess2024-04-30T14:34:25Zoai:repositorio.unesp.br:11449/66000Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-04-30T14:34:25Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Vegetative propagation of Mikania glomerata: Micro-propagation and cuttings |
title |
Vegetative propagation of Mikania glomerata: Micro-propagation and cuttings |
spellingShingle |
Vegetative propagation of Mikania glomerata: Micro-propagation and cuttings Pereira, Ana Maria Soares [UNESP] Guaco Mikania glomerata Stalks Tissue culture |
title_short |
Vegetative propagation of Mikania glomerata: Micro-propagation and cuttings |
title_full |
Vegetative propagation of Mikania glomerata: Micro-propagation and cuttings |
title_fullStr |
Vegetative propagation of Mikania glomerata: Micro-propagation and cuttings |
title_full_unstemmed |
Vegetative propagation of Mikania glomerata: Micro-propagation and cuttings |
title_sort |
Vegetative propagation of Mikania glomerata: Micro-propagation and cuttings |
author |
Pereira, Ana Maria Soares [UNESP] |
author_facet |
Pereira, Ana Maria Soares [UNESP] França, S. C. Câmara, Francisco Luiz Araújo [UNESP] |
author_role |
author |
author2 |
França, S. C. Câmara, Francisco Luiz Araújo [UNESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
UNAERP Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Pereira, Ana Maria Soares [UNESP] França, S. C. Câmara, Francisco Luiz Araújo [UNESP] |
dc.subject.por.fl_str_mv |
Guaco Mikania glomerata Stalks Tissue culture |
topic |
Guaco Mikania glomerata Stalks Tissue culture |
description |
The scope of this work was to compare two systems for vegetative propagation: conventional one (from cut stems) and in vitro micropropagation from axillary buds. Nodal segments (1 cm) of Mikania glomerata were used as explants. The experiments were evaluated in relation to number of shoots; % of rooting; number of roots and total fresh weight. Multiple shoots developed in MS containing 0.5 mg/L BAP. Rooting was induced in the presence of 1.0 mg/L IBA. Stems with five buds and one pair of leaves were the most appropriate for the production of cuttings. The time necessary for developing a protocol for the production of M. glomerata micropropagated plantlets was 6 months, whereas only half time was required to produce plantlets from stem cuttings. The greatest problem met during micropropagation was the culture contamination by endophytic bacteria and fungi. |
publishDate |
1999 |
dc.date.none.fl_str_mv |
1999-12-01 2014-05-27T11:19:50Z 2014-05-27T11:19:50Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/conferenceObject |
format |
conferenceObject |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.actahort.org/books/502/502_57.htm Acta Horticulturae, v. 502, p. 347-352. 0567-7572 http://hdl.handle.net/11449/66000 2-s2.0-84879453141 |
url |
http://www.actahort.org/books/502/502_57.htm http://hdl.handle.net/11449/66000 |
identifier_str_mv |
Acta Horticulturae, v. 502, p. 347-352. 0567-7572 2-s2.0-84879453141 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Acta Horticulturae 0,198 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
347-352 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964888911577088 |