Fungal communities in pressmud composting harbour beneficial and detrimental fungi for human welfare
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1099/mic.0.000306 http://hdl.handle.net/11449/173329 |
Resumo: | Pressmud is a substrate derived from sugarcane juice filtrate, and around 26–40 kg of this residue are produced per ton of sugarcane. It is mainly used as fertilizer in crops, and its application in the field is often made without any prior treatment, but, in this research, it was studied for the risk this practice poses for human health. This research was stimulated by previous results indicating the presence of opportunistic pathogens in residues used in various composting systems and the extensive use of fresh pressmud in agriculture. Here, It was assessed the fungal diversity present in both fresh and composting pressmud using 454 pyrosequencing. In addition, heat-tolerant fungi were isolated and surveyed for their enzymatic repertoire of biomass-degrading enzymes (cellulase, xylanase, laccase and polygalacturonase). A wide range of opportunistic pathogens was found among the most abundant taxa in the fresh pressmud, such as Lomentospora prolificans (43.13 %), Trichosporon sp. (10.07 %), Candida tropicalis (7.91 %), and Hormographiella aspergillata (8.19 %). This indicates that fresh pressmud might be a putative source of human pathogenic fungi, presenting a potential threat to human health if applied as fertilizer without any treatment. With regard to the heat-tolerant fungi found in this substrate, all the 110 isolates screened were able to produce at least one of the tested enzymes. The pressmud composting process not only effectively reduces the load of pathogenic fungi, but also creates an interesting environment for fungi able to produce thermostable hydrolytic and oxidative enzymes with biotechnological applications. |
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Fungal communities in pressmud composting harbour beneficial and detrimental fungi for human welfare454 pyrosequencingFilter cakeLignocelluloseSugarcaneThermophilic fungiPressmud is a substrate derived from sugarcane juice filtrate, and around 26–40 kg of this residue are produced per ton of sugarcane. It is mainly used as fertilizer in crops, and its application in the field is often made without any prior treatment, but, in this research, it was studied for the risk this practice poses for human health. This research was stimulated by previous results indicating the presence of opportunistic pathogens in residues used in various composting systems and the extensive use of fresh pressmud in agriculture. Here, It was assessed the fungal diversity present in both fresh and composting pressmud using 454 pyrosequencing. In addition, heat-tolerant fungi were isolated and surveyed for their enzymatic repertoire of biomass-degrading enzymes (cellulase, xylanase, laccase and polygalacturonase). A wide range of opportunistic pathogens was found among the most abundant taxa in the fresh pressmud, such as Lomentospora prolificans (43.13 %), Trichosporon sp. (10.07 %), Candida tropicalis (7.91 %), and Hormographiella aspergillata (8.19 %). This indicates that fresh pressmud might be a putative source of human pathogenic fungi, presenting a potential threat to human health if applied as fertilizer without any treatment. With regard to the heat-tolerant fungi found in this substrate, all the 110 isolates screened were able to produce at least one of the tested enzymes. The pressmud composting process not only effectively reduces the load of pathogenic fungi, but also creates an interesting environment for fungi able to produce thermostable hydrolytic and oxidative enzymes with biotechnological applications.Department of Biochemistry and Microbiology UNESP -Univ Estadual PaulistaDepartment of Biology UNESP -Univ Estadual PaulistaCenter for the Study of Social Insects UNESP -Univ Estadual PaulistaDivision of Biology and Biological Engineering Caltech -California Institute of TechnologyDepartment of Biochemistry and Microbiology UNESP -Univ Estadual PaulistaDepartment of Biology UNESP -Univ Estadual PaulistaCenter for the Study of Social Insects UNESP -Univ Estadual PaulistaUniversidade Estadual Paulista (Unesp)Caltech -California Institute of Technologyde Oliveira, Tássio Brito [UNESP]Lopes, Viviane Cristina Padilha [UNESP]Barbosa, Fernando Nogueira [UNESP]Ferro, Milene [UNESP]Meirelles, Lucas Andrade [UNESP]Sette, Lara Durães [UNESP]Gomes, Eleni [UNESP]Rodrigues, Andre [UNESP]2018-12-11T17:04:42Z2018-12-11T17:04:42Z2016-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1147-1156application/pdfhttp://dx.doi.org/10.1099/mic.0.000306Microbiology (United Kingdom), v. 162, n. 7, p. 1147-1156, 2016.1465-20801350-0872http://hdl.handle.net/11449/17332910.1099/mic.0.0003062-s2.0-849811648322-s2.0-84981164832.pdf5969653098289575Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMicrobiology (United Kingdom)0,9240,924info:eu-repo/semantics/openAccess2024-01-05T06:21:36Zoai:repositorio.unesp.br:11449/173329Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-05T06:21:36Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Fungal communities in pressmud composting harbour beneficial and detrimental fungi for human welfare |
title |
Fungal communities in pressmud composting harbour beneficial and detrimental fungi for human welfare |
spellingShingle |
Fungal communities in pressmud composting harbour beneficial and detrimental fungi for human welfare de Oliveira, Tássio Brito [UNESP] 454 pyrosequencing Filter cake Lignocellulose Sugarcane Thermophilic fungi |
title_short |
Fungal communities in pressmud composting harbour beneficial and detrimental fungi for human welfare |
title_full |
Fungal communities in pressmud composting harbour beneficial and detrimental fungi for human welfare |
title_fullStr |
Fungal communities in pressmud composting harbour beneficial and detrimental fungi for human welfare |
title_full_unstemmed |
Fungal communities in pressmud composting harbour beneficial and detrimental fungi for human welfare |
title_sort |
Fungal communities in pressmud composting harbour beneficial and detrimental fungi for human welfare |
author |
de Oliveira, Tássio Brito [UNESP] |
author_facet |
de Oliveira, Tássio Brito [UNESP] Lopes, Viviane Cristina Padilha [UNESP] Barbosa, Fernando Nogueira [UNESP] Ferro, Milene [UNESP] Meirelles, Lucas Andrade [UNESP] Sette, Lara Durães [UNESP] Gomes, Eleni [UNESP] Rodrigues, Andre [UNESP] |
author_role |
author |
author2 |
Lopes, Viviane Cristina Padilha [UNESP] Barbosa, Fernando Nogueira [UNESP] Ferro, Milene [UNESP] Meirelles, Lucas Andrade [UNESP] Sette, Lara Durães [UNESP] Gomes, Eleni [UNESP] Rodrigues, Andre [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Caltech -California Institute of Technology |
dc.contributor.author.fl_str_mv |
de Oliveira, Tássio Brito [UNESP] Lopes, Viviane Cristina Padilha [UNESP] Barbosa, Fernando Nogueira [UNESP] Ferro, Milene [UNESP] Meirelles, Lucas Andrade [UNESP] Sette, Lara Durães [UNESP] Gomes, Eleni [UNESP] Rodrigues, Andre [UNESP] |
dc.subject.por.fl_str_mv |
454 pyrosequencing Filter cake Lignocellulose Sugarcane Thermophilic fungi |
topic |
454 pyrosequencing Filter cake Lignocellulose Sugarcane Thermophilic fungi |
description |
Pressmud is a substrate derived from sugarcane juice filtrate, and around 26–40 kg of this residue are produced per ton of sugarcane. It is mainly used as fertilizer in crops, and its application in the field is often made without any prior treatment, but, in this research, it was studied for the risk this practice poses for human health. This research was stimulated by previous results indicating the presence of opportunistic pathogens in residues used in various composting systems and the extensive use of fresh pressmud in agriculture. Here, It was assessed the fungal diversity present in both fresh and composting pressmud using 454 pyrosequencing. In addition, heat-tolerant fungi were isolated and surveyed for their enzymatic repertoire of biomass-degrading enzymes (cellulase, xylanase, laccase and polygalacturonase). A wide range of opportunistic pathogens was found among the most abundant taxa in the fresh pressmud, such as Lomentospora prolificans (43.13 %), Trichosporon sp. (10.07 %), Candida tropicalis (7.91 %), and Hormographiella aspergillata (8.19 %). This indicates that fresh pressmud might be a putative source of human pathogenic fungi, presenting a potential threat to human health if applied as fertilizer without any treatment. With regard to the heat-tolerant fungi found in this substrate, all the 110 isolates screened were able to produce at least one of the tested enzymes. The pressmud composting process not only effectively reduces the load of pathogenic fungi, but also creates an interesting environment for fungi able to produce thermostable hydrolytic and oxidative enzymes with biotechnological applications. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-07-01 2018-12-11T17:04:42Z 2018-12-11T17:04:42Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1099/mic.0.000306 Microbiology (United Kingdom), v. 162, n. 7, p. 1147-1156, 2016. 1465-2080 1350-0872 http://hdl.handle.net/11449/173329 10.1099/mic.0.000306 2-s2.0-84981164832 2-s2.0-84981164832.pdf 5969653098289575 |
url |
http://dx.doi.org/10.1099/mic.0.000306 http://hdl.handle.net/11449/173329 |
identifier_str_mv |
Microbiology (United Kingdom), v. 162, n. 7, p. 1147-1156, 2016. 1465-2080 1350-0872 10.1099/mic.0.000306 2-s2.0-84981164832 2-s2.0-84981164832.pdf 5969653098289575 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Microbiology (United Kingdom) 0,924 0,924 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1147-1156 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1792962258982141952 |