Functional and evolutionary analyses of the miR156 and miR529 families in land plants
Autor(a) principal: | |
---|---|
Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/s12870-016-0716-5 http://hdl.handle.net/11449/168359 |
Resumo: | Background: MicroRNAs (miRNAs) are important regulatory elements of gene expression. Similarly to coding genes, miRNA genes follow a birth and death pattern of evolution likely reflecting functional relevance and divergence. For instance, miRNA529 is evolutionarily related to miRNA156 (a highly conserved miRNA in land plants), but it is lost in Arabidopsis thaliana. Interestingly, both miRNAs target sequences overlap in some members of the SQUAMOSA promoter-binding protein like (SPL) family, raising important questions regarding the diversification of the miR156/miR529-associated regulatory network in land plants. Results: In this study, through phylogenic reconstruction of miR156/529 target sequences from several taxonomic groups, we have found that specific eudicot SPLs, despite miRNA529 loss, retained the corresponding target site. Detailed molecular evolutionary analyses of miR156/miR529-target sequence showed that loss of miR529 in core eudicots, such as Arabidopsis, is correlated with a more relaxed selection of the miRNA529 specific target element, while miRNA156-specific target sequence is under stronger selection, indicating that these two target sites might be under distinct evolutionary constraints. Importantly, over-expression in Arabidopsis of MIR529 precursor from a monocot, but not from a basal eudicot, demonstrates specific miR529 regulation of AtSPL9 and AtSPL15 genes, which contain conserved responsive elements for both miR156 and miR529. Conclusions: Our results suggest loss of functionality of MIR529 genes in the evolutionary history of eudicots and show that the miR529-responsive element present in some eudicot SPLs is still functional. Our data support the notion that particular miRNA156 family members might have compensated for the loss of miR529 regulation in eudicot species, which concomitantly may have favored diversification of eudicot SPLs. |
id |
UNSP_42209dc624fef47dc7966d306c935ddb |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/168359 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Functional and evolutionary analyses of the miR156 and miR529 families in land plantsArabidopsisEudicotsEvolutionMiR156MiR529Background: MicroRNAs (miRNAs) are important regulatory elements of gene expression. Similarly to coding genes, miRNA genes follow a birth and death pattern of evolution likely reflecting functional relevance and divergence. For instance, miRNA529 is evolutionarily related to miRNA156 (a highly conserved miRNA in land plants), but it is lost in Arabidopsis thaliana. Interestingly, both miRNAs target sequences overlap in some members of the SQUAMOSA promoter-binding protein like (SPL) family, raising important questions regarding the diversification of the miR156/miR529-associated regulatory network in land plants. Results: In this study, through phylogenic reconstruction of miR156/529 target sequences from several taxonomic groups, we have found that specific eudicot SPLs, despite miRNA529 loss, retained the corresponding target site. Detailed molecular evolutionary analyses of miR156/miR529-target sequence showed that loss of miR529 in core eudicots, such as Arabidopsis, is correlated with a more relaxed selection of the miRNA529 specific target element, while miRNA156-specific target sequence is under stronger selection, indicating that these two target sites might be under distinct evolutionary constraints. Importantly, over-expression in Arabidopsis of MIR529 precursor from a monocot, but not from a basal eudicot, demonstrates specific miR529 regulation of AtSPL9 and AtSPL15 genes, which contain conserved responsive elements for both miR156 and miR529. Conclusions: Our results suggest loss of functionality of MIR529 genes in the evolutionary history of eudicots and show that the miR529-responsive element present in some eudicot SPLs is still functional. Our data support the notion that particular miRNA156 family members might have compensated for the loss of miR529 regulation in eudicot species, which concomitantly may have favored diversification of eudicot SPLs.Escola Superior de Agricultura 'Luiz de Queiroz' Universidade de Sao Paulo Laboratório de Genetica Molecular do Desenvolvimento Vegetal Departamento de Ciencias Biológicas, Avenida Padua Dias, 11Instituto de Biociencias Universidade Estadual Paulista (UNESP) Departamento de GenéticaUniversidade Estadual Paulista (UNESP) Departmento de Bioprocessos e BiotecnologiaCentro de Biologia Molecular e Engenharia Genetica (CBMEG) Universidade Estadual de CampinasInstituto de Biociencias Universidade Estadual Paulista (UNESP) Departamento de GenéticaUniversidade Estadual Paulista (UNESP) Departmento de Bioprocessos e BiotecnologiaUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)Morea, Edna Gicela Ortiz [UNESP]da Silva, Eder Marquese Silva, Geraldo Felipe FerreiraValente, Guilherme Targino [UNESP]Barrera Rojas, Carlos Hernan [UNESP]Vincentz, MichelNogueira, Fabio Tebaldi Siveira2018-12-11T16:40:56Z2018-12-11T16:40:56Z2016-02-03info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1186/s12870-016-0716-5BMC Plant Biology, v. 16, n. 1, 2016.1471-2229http://hdl.handle.net/11449/16835910.1186/s12870-016-0716-52-s2.0-849574403632-s2.0-84957440363.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBMC Plant Biology1,887info:eu-repo/semantics/openAccess2023-10-20T06:06:59Zoai:repositorio.unesp.br:11449/168359Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-20T06:06:59Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Functional and evolutionary analyses of the miR156 and miR529 families in land plants |
title |
Functional and evolutionary analyses of the miR156 and miR529 families in land plants |
spellingShingle |
Functional and evolutionary analyses of the miR156 and miR529 families in land plants Morea, Edna Gicela Ortiz [UNESP] Arabidopsis Eudicots Evolution MiR156 MiR529 |
title_short |
Functional and evolutionary analyses of the miR156 and miR529 families in land plants |
title_full |
Functional and evolutionary analyses of the miR156 and miR529 families in land plants |
title_fullStr |
Functional and evolutionary analyses of the miR156 and miR529 families in land plants |
title_full_unstemmed |
Functional and evolutionary analyses of the miR156 and miR529 families in land plants |
title_sort |
Functional and evolutionary analyses of the miR156 and miR529 families in land plants |
author |
Morea, Edna Gicela Ortiz [UNESP] |
author_facet |
Morea, Edna Gicela Ortiz [UNESP] da Silva, Eder Marques e Silva, Geraldo Felipe Ferreira Valente, Guilherme Targino [UNESP] Barrera Rojas, Carlos Hernan [UNESP] Vincentz, Michel Nogueira, Fabio Tebaldi Siveira |
author_role |
author |
author2 |
da Silva, Eder Marques e Silva, Geraldo Felipe Ferreira Valente, Guilherme Targino [UNESP] Barrera Rojas, Carlos Hernan [UNESP] Vincentz, Michel Nogueira, Fabio Tebaldi Siveira |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) Universidade Estadual de Campinas (UNICAMP) |
dc.contributor.author.fl_str_mv |
Morea, Edna Gicela Ortiz [UNESP] da Silva, Eder Marques e Silva, Geraldo Felipe Ferreira Valente, Guilherme Targino [UNESP] Barrera Rojas, Carlos Hernan [UNESP] Vincentz, Michel Nogueira, Fabio Tebaldi Siveira |
dc.subject.por.fl_str_mv |
Arabidopsis Eudicots Evolution MiR156 MiR529 |
topic |
Arabidopsis Eudicots Evolution MiR156 MiR529 |
description |
Background: MicroRNAs (miRNAs) are important regulatory elements of gene expression. Similarly to coding genes, miRNA genes follow a birth and death pattern of evolution likely reflecting functional relevance and divergence. For instance, miRNA529 is evolutionarily related to miRNA156 (a highly conserved miRNA in land plants), but it is lost in Arabidopsis thaliana. Interestingly, both miRNAs target sequences overlap in some members of the SQUAMOSA promoter-binding protein like (SPL) family, raising important questions regarding the diversification of the miR156/miR529-associated regulatory network in land plants. Results: In this study, through phylogenic reconstruction of miR156/529 target sequences from several taxonomic groups, we have found that specific eudicot SPLs, despite miRNA529 loss, retained the corresponding target site. Detailed molecular evolutionary analyses of miR156/miR529-target sequence showed that loss of miR529 in core eudicots, such as Arabidopsis, is correlated with a more relaxed selection of the miRNA529 specific target element, while miRNA156-specific target sequence is under stronger selection, indicating that these two target sites might be under distinct evolutionary constraints. Importantly, over-expression in Arabidopsis of MIR529 precursor from a monocot, but not from a basal eudicot, demonstrates specific miR529 regulation of AtSPL9 and AtSPL15 genes, which contain conserved responsive elements for both miR156 and miR529. Conclusions: Our results suggest loss of functionality of MIR529 genes in the evolutionary history of eudicots and show that the miR529-responsive element present in some eudicot SPLs is still functional. Our data support the notion that particular miRNA156 family members might have compensated for the loss of miR529 regulation in eudicot species, which concomitantly may have favored diversification of eudicot SPLs. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-02-03 2018-12-11T16:40:56Z 2018-12-11T16:40:56Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/s12870-016-0716-5 BMC Plant Biology, v. 16, n. 1, 2016. 1471-2229 http://hdl.handle.net/11449/168359 10.1186/s12870-016-0716-5 2-s2.0-84957440363 2-s2.0-84957440363.pdf |
url |
http://dx.doi.org/10.1186/s12870-016-0716-5 http://hdl.handle.net/11449/168359 |
identifier_str_mv |
BMC Plant Biology, v. 16, n. 1, 2016. 1471-2229 10.1186/s12870-016-0716-5 2-s2.0-84957440363 2-s2.0-84957440363.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
BMC Plant Biology 1,887 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964632117411840 |