Separation and purification of curcumin using novel aqueous two-phase micellar systems composed of amphiphilic copolymer and cholinium ionic liquids
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.seppur.2020.117262 http://hdl.handle.net/11449/199026 |
Resumo: | Novel aqueous two-phase micellar systems (ATPMS) composed of Pluronic F68, a triblock amphiphilic copolymer, and cholinium-based ionic liquids (ILs) were formulated and applied for separation/purification of curcumin (CCM). CCM stability in the presence of ATPMS components was also evaluated. CCM is stable up to 24 h in copolymer (1.0 – 10.0 wt%) and ILs (0.1 – 3.0 M) aqueous solutions. Very mild phase separation conditions (close to room temperature) were achieved by adding cholinium ILs to the Pluronic F68 + McIlvaine buffer at pH 6.0 solution. The decrease of cloud-point temperature is dependent on the relative hydrophobicity of IL anion, [Hex]− > [But]− > [Pro]− > [Ac]− > Cl−. ATPMS composed of more hydrophobic ILs ([Ch][Hex] > [Ch][But] > [Ch][Pro]) are most efficient in the partition of commercial CCM into polymeric micelles (PMs)-rich phase. The best ATPMS (0.70 M [Ch][But] and 0.60 M [Ch][Hex]-based ATPMS) were then used to purify CCM from a crude extract of Curcuma longa L. Both systems were very selective to separate CCM from protein-based contaminants (selectivity values ≥ 25; purification yields ≥ 12-fold). Pluronic F68-based ATPMS are promising for selective separation of hydrophobic biomolecules by using cholinium-based ILs as adjuvants to adjust phase separation temperatures and biomolecules’ partition. |
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Repositório Institucional da UNESP |
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Separation and purification of curcumin using novel aqueous two-phase micellar systems composed of amphiphilic copolymer and cholinium ionic liquidsaqueous two-phase micellar systems (ATPMS)curcumin (CCM)ionic liquids (ILs)Pluronic F68polymeric micelles (PMs)separationNovel aqueous two-phase micellar systems (ATPMS) composed of Pluronic F68, a triblock amphiphilic copolymer, and cholinium-based ionic liquids (ILs) were formulated and applied for separation/purification of curcumin (CCM). CCM stability in the presence of ATPMS components was also evaluated. CCM is stable up to 24 h in copolymer (1.0 – 10.0 wt%) and ILs (0.1 – 3.0 M) aqueous solutions. Very mild phase separation conditions (close to room temperature) were achieved by adding cholinium ILs to the Pluronic F68 + McIlvaine buffer at pH 6.0 solution. The decrease of cloud-point temperature is dependent on the relative hydrophobicity of IL anion, [Hex]− > [But]− > [Pro]− > [Ac]− > Cl−. ATPMS composed of more hydrophobic ILs ([Ch][Hex] > [Ch][But] > [Ch][Pro]) are most efficient in the partition of commercial CCM into polymeric micelles (PMs)-rich phase. The best ATPMS (0.70 M [Ch][But] and 0.60 M [Ch][Hex]-based ATPMS) were then used to purify CCM from a crude extract of Curcuma longa L. Both systems were very selective to separate CCM from protein-based contaminants (selectivity values ≥ 25; purification yields ≥ 12-fold). Pluronic F68-based ATPMS are promising for selective separation of hydrophobic biomolecules by using cholinium-based ILs as adjuvants to adjust phase separation temperatures and biomolecules’ partition.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Engineering Bioprocesses and Biotechnology School of Pharmaceutical Sciences São Paulo State University (UNESP)Faculty of Pharmaceutical Sciences University of CampinasCentre of Biological Engineering (CEB) University of Minho, Campus de GualtarUniv Coimbra CIEPQPF Department of Chemical Engineering Rua Sílvio Lima, Pólo II – Pinhal de MarrocosDepartment of Engineering Bioprocesses and Biotechnology School of Pharmaceutical Sciences São Paulo State University (UNESP)CAPES: 001FAPESP: 2014/16424-7FAPESP: 2017/10789-1FAPESP: 2018/05111-9FAPESP: 2018/10799-0FAPESP: 2019/05624-9FAPESP: 2019/08549-8Universidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)University of MinhoRua Sílvio LimaKurnik, Isabelle S. [UNESP]Noronha, Mariana A.Câmara, Mayra C.C.Mazzola, Priscila G.Vicente, António A.Pereira, Jorge F.B. [UNESP]Lopes, André M.2020-12-12T01:28:47Z2020-12-12T01:28:47Z2020-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.seppur.2020.117262Separation and Purification Technology, v. 250.1873-37941383-5866http://hdl.handle.net/11449/19902610.1016/j.seppur.2020.1172622-s2.0-85086876741Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengSeparation and Purification Technologyinfo:eu-repo/semantics/openAccess2021-10-23T00:57:10Zoai:repositorio.unesp.br:11449/199026Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T00:57:10Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Separation and purification of curcumin using novel aqueous two-phase micellar systems composed of amphiphilic copolymer and cholinium ionic liquids |
title |
Separation and purification of curcumin using novel aqueous two-phase micellar systems composed of amphiphilic copolymer and cholinium ionic liquids |
spellingShingle |
Separation and purification of curcumin using novel aqueous two-phase micellar systems composed of amphiphilic copolymer and cholinium ionic liquids Kurnik, Isabelle S. [UNESP] aqueous two-phase micellar systems (ATPMS) curcumin (CCM) ionic liquids (ILs) Pluronic F68 polymeric micelles (PMs) separation |
title_short |
Separation and purification of curcumin using novel aqueous two-phase micellar systems composed of amphiphilic copolymer and cholinium ionic liquids |
title_full |
Separation and purification of curcumin using novel aqueous two-phase micellar systems composed of amphiphilic copolymer and cholinium ionic liquids |
title_fullStr |
Separation and purification of curcumin using novel aqueous two-phase micellar systems composed of amphiphilic copolymer and cholinium ionic liquids |
title_full_unstemmed |
Separation and purification of curcumin using novel aqueous two-phase micellar systems composed of amphiphilic copolymer and cholinium ionic liquids |
title_sort |
Separation and purification of curcumin using novel aqueous two-phase micellar systems composed of amphiphilic copolymer and cholinium ionic liquids |
author |
Kurnik, Isabelle S. [UNESP] |
author_facet |
Kurnik, Isabelle S. [UNESP] Noronha, Mariana A. Câmara, Mayra C.C. Mazzola, Priscila G. Vicente, António A. Pereira, Jorge F.B. [UNESP] Lopes, André M. |
author_role |
author |
author2 |
Noronha, Mariana A. Câmara, Mayra C.C. Mazzola, Priscila G. Vicente, António A. Pereira, Jorge F.B. [UNESP] Lopes, André M. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Estadual de Campinas (UNICAMP) University of Minho Rua Sílvio Lima |
dc.contributor.author.fl_str_mv |
Kurnik, Isabelle S. [UNESP] Noronha, Mariana A. Câmara, Mayra C.C. Mazzola, Priscila G. Vicente, António A. Pereira, Jorge F.B. [UNESP] Lopes, André M. |
dc.subject.por.fl_str_mv |
aqueous two-phase micellar systems (ATPMS) curcumin (CCM) ionic liquids (ILs) Pluronic F68 polymeric micelles (PMs) separation |
topic |
aqueous two-phase micellar systems (ATPMS) curcumin (CCM) ionic liquids (ILs) Pluronic F68 polymeric micelles (PMs) separation |
description |
Novel aqueous two-phase micellar systems (ATPMS) composed of Pluronic F68, a triblock amphiphilic copolymer, and cholinium-based ionic liquids (ILs) were formulated and applied for separation/purification of curcumin (CCM). CCM stability in the presence of ATPMS components was also evaluated. CCM is stable up to 24 h in copolymer (1.0 – 10.0 wt%) and ILs (0.1 – 3.0 M) aqueous solutions. Very mild phase separation conditions (close to room temperature) were achieved by adding cholinium ILs to the Pluronic F68 + McIlvaine buffer at pH 6.0 solution. The decrease of cloud-point temperature is dependent on the relative hydrophobicity of IL anion, [Hex]− > [But]− > [Pro]− > [Ac]− > Cl−. ATPMS composed of more hydrophobic ILs ([Ch][Hex] > [Ch][But] > [Ch][Pro]) are most efficient in the partition of commercial CCM into polymeric micelles (PMs)-rich phase. The best ATPMS (0.70 M [Ch][But] and 0.60 M [Ch][Hex]-based ATPMS) were then used to purify CCM from a crude extract of Curcuma longa L. Both systems were very selective to separate CCM from protein-based contaminants (selectivity values ≥ 25; purification yields ≥ 12-fold). Pluronic F68-based ATPMS are promising for selective separation of hydrophobic biomolecules by using cholinium-based ILs as adjuvants to adjust phase separation temperatures and biomolecules’ partition. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T01:28:47Z 2020-12-12T01:28:47Z 2020-11-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.seppur.2020.117262 Separation and Purification Technology, v. 250. 1873-3794 1383-5866 http://hdl.handle.net/11449/199026 10.1016/j.seppur.2020.117262 2-s2.0-85086876741 |
url |
http://dx.doi.org/10.1016/j.seppur.2020.117262 http://hdl.handle.net/11449/199026 |
identifier_str_mv |
Separation and Purification Technology, v. 250. 1873-3794 1383-5866 10.1016/j.seppur.2020.117262 2-s2.0-85086876741 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Separation and Purification Technology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965071202320384 |