Evaluation of tissue reaction, cell viability and cytokine production induced by Sealapex Plus
Autor(a) principal: | |
---|---|
Data de Publicação: | 2011 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1678-77572011005000006 http://hdl.handle.net/11449/72575 |
Resumo: | The aim of this study was to investigate the effects of mineral trioxide aggregate (MTA), Sealapex, and a combination of Sealapex and MTA (Sealapex Plus) on the reaction of subcutaneous connective tissue of rats, and on cell viability and cytokine production in mouse fibroblasts. The tissue reaction was carried out with dentin tubes containing the materials implanted in the dorsal connective tissue of rats. The histological analysis was performed after 7 and 30 days. Millipore culture plate inserts with polyethylene tubes filled with materials were placed into 24-well cell culture plates with mouse fibroblasts to evaluate the cell viability by MTT assay. ELISA assays were also performed after 24 h of exposure of the mouse fibroblasts to set material disks. Histopathologic examination showed Von Kossa-positive granules that were birefringent to polarized light for all the studied materials at the tube openings. No material inhibited the cell viability in the in vitro test. It was detected IL-6 production in all root-end filling materials. MTA and Sealapex Plus induced a slight raise of mean levels of IL-1β. The results suggest that Sealapex Plus is biocompatible and stimulates the mineralization of the tissue. |
id |
UNSP_473fb9ca4afca13a851a3a3b7bc8583c |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/72575 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Evaluation of tissue reaction, cell viability and cytokine production induced by Sealapex Plusaluminum derivativebiomaterialcalcium derivativecalcium hydroxidecytokinemineral trioxide aggregateoxideroot canal filling materialsalicylic acid derivativesealapexsilicateanimalcell culturecell survivalconnective tissuedentindrug combinationdrug effectenzyme linked immunosorbent assayfibroblastmalematerials testingrattimeWistar ratAluminum CompoundsAnimalsBiocompatible MaterialsCalcium CompoundsCalcium HydroxideCell SurvivalCells, CulturedConnective TissueCytokinesDentinDrug CombinationsEnzyme-Linked Immunosorbent AssayFibroblastsMaleMaterials TestingOxidesRatsRats, WistarRoot Canal Filling MaterialsSalicylatesSilicatesTime FactorsThe aim of this study was to investigate the effects of mineral trioxide aggregate (MTA), Sealapex, and a combination of Sealapex and MTA (Sealapex Plus) on the reaction of subcutaneous connective tissue of rats, and on cell viability and cytokine production in mouse fibroblasts. The tissue reaction was carried out with dentin tubes containing the materials implanted in the dorsal connective tissue of rats. The histological analysis was performed after 7 and 30 days. Millipore culture plate inserts with polyethylene tubes filled with materials were placed into 24-well cell culture plates with mouse fibroblasts to evaluate the cell viability by MTT assay. ELISA assays were also performed after 24 h of exposure of the mouse fibroblasts to set material disks. Histopathologic examination showed Von Kossa-positive granules that were birefringent to polarized light for all the studied materials at the tube openings. No material inhibited the cell viability in the in vitro test. It was detected IL-6 production in all root-end filling materials. MTA and Sealapex Plus induced a slight raise of mean levels of IL-1β. The results suggest that Sealapex Plus is biocompatible and stimulates the mineralization of the tissue.Universidade Estadual Paulista (Unesp)Gomes-Filho, João Eduardo [UNESP]Gomes, Alessandra Cristina [UNESP]Watanabe, Simone [UNESP]Oliveira, Sandra Helena Penha de [UNESP]Bernabé, Pedro Felício Estrada [UNESP]Percinoto, Célio [UNESP]2014-05-27T11:25:57Z2014-05-27T11:25:57Z2011-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article329-336application/pdfhttp://dx.doi.org/10.1590/S1678-77572011005000006Journal of applied oral science: revista FOB, v. 19, n. 4, p. 329-336, 2011.1678-7765http://hdl.handle.net/11449/7257510.1590/S1678-77572011005000006S1678-77572011005000006WOS:0002990023000072-s2.0-848563931572-s2.0-84856393157.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of applied oral science : revista FOBinfo:eu-repo/semantics/openAccess2024-01-05T06:21:45Zoai:repositorio.unesp.br:11449/72575Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-05T06:21:45Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Evaluation of tissue reaction, cell viability and cytokine production induced by Sealapex Plus |
title |
Evaluation of tissue reaction, cell viability and cytokine production induced by Sealapex Plus |
spellingShingle |
Evaluation of tissue reaction, cell viability and cytokine production induced by Sealapex Plus Gomes-Filho, João Eduardo [UNESP] aluminum derivative biomaterial calcium derivative calcium hydroxide cytokine mineral trioxide aggregate oxide root canal filling material salicylic acid derivative sealapex silicate animal cell culture cell survival connective tissue dentin drug combination drug effect enzyme linked immunosorbent assay fibroblast male materials testing rat time Wistar rat Aluminum Compounds Animals Biocompatible Materials Calcium Compounds Calcium Hydroxide Cell Survival Cells, Cultured Connective Tissue Cytokines Dentin Drug Combinations Enzyme-Linked Immunosorbent Assay Fibroblasts Male Materials Testing Oxides Rats Rats, Wistar Root Canal Filling Materials Salicylates Silicates Time Factors |
title_short |
Evaluation of tissue reaction, cell viability and cytokine production induced by Sealapex Plus |
title_full |
Evaluation of tissue reaction, cell viability and cytokine production induced by Sealapex Plus |
title_fullStr |
Evaluation of tissue reaction, cell viability and cytokine production induced by Sealapex Plus |
title_full_unstemmed |
Evaluation of tissue reaction, cell viability and cytokine production induced by Sealapex Plus |
title_sort |
Evaluation of tissue reaction, cell viability and cytokine production induced by Sealapex Plus |
author |
Gomes-Filho, João Eduardo [UNESP] |
author_facet |
Gomes-Filho, João Eduardo [UNESP] Gomes, Alessandra Cristina [UNESP] Watanabe, Simone [UNESP] Oliveira, Sandra Helena Penha de [UNESP] Bernabé, Pedro Felício Estrada [UNESP] Percinoto, Célio [UNESP] |
author_role |
author |
author2 |
Gomes, Alessandra Cristina [UNESP] Watanabe, Simone [UNESP] Oliveira, Sandra Helena Penha de [UNESP] Bernabé, Pedro Felício Estrada [UNESP] Percinoto, Célio [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Gomes-Filho, João Eduardo [UNESP] Gomes, Alessandra Cristina [UNESP] Watanabe, Simone [UNESP] Oliveira, Sandra Helena Penha de [UNESP] Bernabé, Pedro Felício Estrada [UNESP] Percinoto, Célio [UNESP] |
dc.subject.por.fl_str_mv |
aluminum derivative biomaterial calcium derivative calcium hydroxide cytokine mineral trioxide aggregate oxide root canal filling material salicylic acid derivative sealapex silicate animal cell culture cell survival connective tissue dentin drug combination drug effect enzyme linked immunosorbent assay fibroblast male materials testing rat time Wistar rat Aluminum Compounds Animals Biocompatible Materials Calcium Compounds Calcium Hydroxide Cell Survival Cells, Cultured Connective Tissue Cytokines Dentin Drug Combinations Enzyme-Linked Immunosorbent Assay Fibroblasts Male Materials Testing Oxides Rats Rats, Wistar Root Canal Filling Materials Salicylates Silicates Time Factors |
topic |
aluminum derivative biomaterial calcium derivative calcium hydroxide cytokine mineral trioxide aggregate oxide root canal filling material salicylic acid derivative sealapex silicate animal cell culture cell survival connective tissue dentin drug combination drug effect enzyme linked immunosorbent assay fibroblast male materials testing rat time Wistar rat Aluminum Compounds Animals Biocompatible Materials Calcium Compounds Calcium Hydroxide Cell Survival Cells, Cultured Connective Tissue Cytokines Dentin Drug Combinations Enzyme-Linked Immunosorbent Assay Fibroblasts Male Materials Testing Oxides Rats Rats, Wistar Root Canal Filling Materials Salicylates Silicates Time Factors |
description |
The aim of this study was to investigate the effects of mineral trioxide aggregate (MTA), Sealapex, and a combination of Sealapex and MTA (Sealapex Plus) on the reaction of subcutaneous connective tissue of rats, and on cell viability and cytokine production in mouse fibroblasts. The tissue reaction was carried out with dentin tubes containing the materials implanted in the dorsal connective tissue of rats. The histological analysis was performed after 7 and 30 days. Millipore culture plate inserts with polyethylene tubes filled with materials were placed into 24-well cell culture plates with mouse fibroblasts to evaluate the cell viability by MTT assay. ELISA assays were also performed after 24 h of exposure of the mouse fibroblasts to set material disks. Histopathologic examination showed Von Kossa-positive granules that were birefringent to polarized light for all the studied materials at the tube openings. No material inhibited the cell viability in the in vitro test. It was detected IL-6 production in all root-end filling materials. MTA and Sealapex Plus induced a slight raise of mean levels of IL-1β. The results suggest that Sealapex Plus is biocompatible and stimulates the mineralization of the tissue. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-08-01 2014-05-27T11:25:57Z 2014-05-27T11:25:57Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1678-77572011005000006 Journal of applied oral science: revista FOB, v. 19, n. 4, p. 329-336, 2011. 1678-7765 http://hdl.handle.net/11449/72575 10.1590/S1678-77572011005000006 S1678-77572011005000006 WOS:000299002300007 2-s2.0-84856393157 2-s2.0-84856393157.pdf |
url |
http://dx.doi.org/10.1590/S1678-77572011005000006 http://hdl.handle.net/11449/72575 |
identifier_str_mv |
Journal of applied oral science: revista FOB, v. 19, n. 4, p. 329-336, 2011. 1678-7765 10.1590/S1678-77572011005000006 S1678-77572011005000006 WOS:000299002300007 2-s2.0-84856393157 2-s2.0-84856393157.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of applied oral science : revista FOB |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
329-336 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797790205676093440 |