Testicular alterations in cryptorchid/orchiopexic rats chronically exposed to acrylamide or di-butyl-phthalate
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1293/tox.2021-0045 http://hdl.handle.net/11449/241126 |
Resumo: | Exposure of Sprague-Dawley (SD) rats to acrylamide (AA) or di-butyl-phthalate (DBP) from the 12th gestational day to the 16th postnatal week (PNW) has been shown to reduce the effectiveness of orchiopexy in recovering the testicular alterations associated with experimental cryptorchidism established at weaning. Herein, we provide information about the long-term effects of AA or DBP on the testes of cryptorchid/orchiopexic rats. Male offspring exposed in utero to 10 mg/kg/day AA or 500 mg/kg/day DBP underwent bilateral surgical cryptorchidism at the 3rd PNW and orchiopexy at the 6th week, with continuous exposure to the chemicals through diet until the 58th week. Regardless of the test chemical, there were severe qualitative/quantitative alterations in the seminiferous tubules and increased numbers of Leydig cells. There was an increase and decrease in the number of tubules with c-Kit- and placental alkaline phosphatase-labeled germ cells, respectively, as compared to those in the control group, suggesting an imbalance between apoptosis and cell proliferation processes. The histological scores of the testicular lesions at the end of this one-year study were higher than those in the previous 16-week study, indicating that exposure of rats to the toxicants AA or DBP enhanced the testicular alterations induced by the chemicals beginning at the intra-uterine life, and impaired the effectiveness of orchiopexy in restoring the testes to normal morphology. Although the present experimental protocol does not completely replicate the natural human undescended testes, our findings may contribute to understanding the alterations occurring in cryptorchid/orchiopexic testes potentially exposed to exogenous chemicals for extended periods. |
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Testicular alterations in cryptorchid/orchiopexic rats chronically exposed to acrylamide or di-butyl-phthalateAcrylamideChronic toxicityDi-butyl-phthalateOrchiopexyRat surgical cryptorchidismTesticular germ cellsExposure of Sprague-Dawley (SD) rats to acrylamide (AA) or di-butyl-phthalate (DBP) from the 12th gestational day to the 16th postnatal week (PNW) has been shown to reduce the effectiveness of orchiopexy in recovering the testicular alterations associated with experimental cryptorchidism established at weaning. Herein, we provide information about the long-term effects of AA or DBP on the testes of cryptorchid/orchiopexic rats. Male offspring exposed in utero to 10 mg/kg/day AA or 500 mg/kg/day DBP underwent bilateral surgical cryptorchidism at the 3rd PNW and orchiopexy at the 6th week, with continuous exposure to the chemicals through diet until the 58th week. Regardless of the test chemical, there were severe qualitative/quantitative alterations in the seminiferous tubules and increased numbers of Leydig cells. There was an increase and decrease in the number of tubules with c-Kit- and placental alkaline phosphatase-labeled germ cells, respectively, as compared to those in the control group, suggesting an imbalance between apoptosis and cell proliferation processes. The histological scores of the testicular lesions at the end of this one-year study were higher than those in the previous 16-week study, indicating that exposure of rats to the toxicants AA or DBP enhanced the testicular alterations induced by the chemicals beginning at the intra-uterine life, and impaired the effectiveness of orchiopexy in restoring the testes to normal morphology. Although the present experimental protocol does not completely replicate the natural human undescended testes, our findings may contribute to understanding the alterations occurring in cryptorchid/orchiopexic testes potentially exposed to exogenous chemicals for extended periods.Center for Evaluation of Environmental Impact on Human Health (TOXICAM) Department of Pathology Botucatu Medical School São Paulo State University (UNESP)Allergisa Pesquisa Dermato CosméticaDepartment of Pharmacology and Toxicology University of LouisvilleCentro Universitário Sudoeste Paulista (UniFSP)Syngenta Crop Protection São PauloDepartment of Dermatology and Radiotherapy Botucatu Medical School São Paulo State University (UNESP)Department of Pathology and Microbiology University of Nebraska Medical CenterCenter for Evaluation of Environmental Impact on Human Health (TOXICAM) Department of Pathology Botucatu Medical School São Paulo State University (UNESP)Department of Dermatology and Radiotherapy Botucatu Medical School São Paulo State University (UNESP)Universidade Estadual Paulista (UNESP)Allergisa Pesquisa Dermato CosméticaUniversity of LouisvilleCentro Universitário Sudoeste Paulista (UniFSP)São PauloUniversity of Nebraska Medical CenterLima, Thania R. R. [UNESP]Souza, Nathália P. [UNESP]Cardoso, Ana P. Ferragut [UNESP]Gomide, Lígia M. M. [UNESP]Pontes, Merielen G. Nascimento E. [UNESP]Miot, Hélio A. [UNESP]Arnold, Lora L.Cohen, Samuel M.De Camargo, João Lauro V. [UNESP]2023-03-01T20:48:16Z2023-03-01T20:48:16Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article159-170http://dx.doi.org/10.1293/tox.2021-0045Journal of Toxicologic Pathology, v. 35, n. 2, p. 159-170, 2022.0914-9198http://hdl.handle.net/11449/24112610.1293/tox.2021-00452-s2.0-85131538562Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Toxicologic Pathologyinfo:eu-repo/semantics/openAccess2023-03-01T20:48:16Zoai:repositorio.unesp.br:11449/241126Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-03-01T20:48:16Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Testicular alterations in cryptorchid/orchiopexic rats chronically exposed to acrylamide or di-butyl-phthalate |
title |
Testicular alterations in cryptorchid/orchiopexic rats chronically exposed to acrylamide or di-butyl-phthalate |
spellingShingle |
Testicular alterations in cryptorchid/orchiopexic rats chronically exposed to acrylamide or di-butyl-phthalate Lima, Thania R. R. [UNESP] Acrylamide Chronic toxicity Di-butyl-phthalate Orchiopexy Rat surgical cryptorchidism Testicular germ cells |
title_short |
Testicular alterations in cryptorchid/orchiopexic rats chronically exposed to acrylamide or di-butyl-phthalate |
title_full |
Testicular alterations in cryptorchid/orchiopexic rats chronically exposed to acrylamide or di-butyl-phthalate |
title_fullStr |
Testicular alterations in cryptorchid/orchiopexic rats chronically exposed to acrylamide or di-butyl-phthalate |
title_full_unstemmed |
Testicular alterations in cryptorchid/orchiopexic rats chronically exposed to acrylamide or di-butyl-phthalate |
title_sort |
Testicular alterations in cryptorchid/orchiopexic rats chronically exposed to acrylamide or di-butyl-phthalate |
author |
Lima, Thania R. R. [UNESP] |
author_facet |
Lima, Thania R. R. [UNESP] Souza, Nathália P. [UNESP] Cardoso, Ana P. Ferragut [UNESP] Gomide, Lígia M. M. [UNESP] Pontes, Merielen G. Nascimento E. [UNESP] Miot, Hélio A. [UNESP] Arnold, Lora L. Cohen, Samuel M. De Camargo, João Lauro V. [UNESP] |
author_role |
author |
author2 |
Souza, Nathália P. [UNESP] Cardoso, Ana P. Ferragut [UNESP] Gomide, Lígia M. M. [UNESP] Pontes, Merielen G. Nascimento E. [UNESP] Miot, Hélio A. [UNESP] Arnold, Lora L. Cohen, Samuel M. De Camargo, João Lauro V. [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Allergisa Pesquisa Dermato Cosmética University of Louisville Centro Universitário Sudoeste Paulista (UniFSP) São Paulo University of Nebraska Medical Center |
dc.contributor.author.fl_str_mv |
Lima, Thania R. R. [UNESP] Souza, Nathália P. [UNESP] Cardoso, Ana P. Ferragut [UNESP] Gomide, Lígia M. M. [UNESP] Pontes, Merielen G. Nascimento E. [UNESP] Miot, Hélio A. [UNESP] Arnold, Lora L. Cohen, Samuel M. De Camargo, João Lauro V. [UNESP] |
dc.subject.por.fl_str_mv |
Acrylamide Chronic toxicity Di-butyl-phthalate Orchiopexy Rat surgical cryptorchidism Testicular germ cells |
topic |
Acrylamide Chronic toxicity Di-butyl-phthalate Orchiopexy Rat surgical cryptorchidism Testicular germ cells |
description |
Exposure of Sprague-Dawley (SD) rats to acrylamide (AA) or di-butyl-phthalate (DBP) from the 12th gestational day to the 16th postnatal week (PNW) has been shown to reduce the effectiveness of orchiopexy in recovering the testicular alterations associated with experimental cryptorchidism established at weaning. Herein, we provide information about the long-term effects of AA or DBP on the testes of cryptorchid/orchiopexic rats. Male offspring exposed in utero to 10 mg/kg/day AA or 500 mg/kg/day DBP underwent bilateral surgical cryptorchidism at the 3rd PNW and orchiopexy at the 6th week, with continuous exposure to the chemicals through diet until the 58th week. Regardless of the test chemical, there were severe qualitative/quantitative alterations in the seminiferous tubules and increased numbers of Leydig cells. There was an increase and decrease in the number of tubules with c-Kit- and placental alkaline phosphatase-labeled germ cells, respectively, as compared to those in the control group, suggesting an imbalance between apoptosis and cell proliferation processes. The histological scores of the testicular lesions at the end of this one-year study were higher than those in the previous 16-week study, indicating that exposure of rats to the toxicants AA or DBP enhanced the testicular alterations induced by the chemicals beginning at the intra-uterine life, and impaired the effectiveness of orchiopexy in restoring the testes to normal morphology. Although the present experimental protocol does not completely replicate the natural human undescended testes, our findings may contribute to understanding the alterations occurring in cryptorchid/orchiopexic testes potentially exposed to exogenous chemicals for extended periods. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01 2023-03-01T20:48:16Z 2023-03-01T20:48:16Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1293/tox.2021-0045 Journal of Toxicologic Pathology, v. 35, n. 2, p. 159-170, 2022. 0914-9198 http://hdl.handle.net/11449/241126 10.1293/tox.2021-0045 2-s2.0-85131538562 |
url |
http://dx.doi.org/10.1293/tox.2021-0045 http://hdl.handle.net/11449/241126 |
identifier_str_mv |
Journal of Toxicologic Pathology, v. 35, n. 2, p. 159-170, 2022. 0914-9198 10.1293/tox.2021-0045 2-s2.0-85131538562 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Toxicologic Pathology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
159-170 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797789955901095936 |