Phenotypic characterization of regulatory T cells populations in maternal blood, cord blood and placenta from diabetic mothers
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1080/14767058.2017.1400004 http://hdl.handle.net/11449/177087 |
Resumo: | Purpose: Changes in regulatory T cells (Treg) in peripheral blood are associated with a number of pathologies, including diabetes. However, the immunological responses of pregnant diabetic women remain scarcely known, and the effects of Treg cells in these patients have yet to be investigated. The present study characterized the expression of regulatory T cells in the maternal blood, cord blood and placenta of diabetic pregnant women. Materials and methods: The women were divided according to glycemic status into a non-diabetic (ND; N = 20) or type 2 diabetic (T2DM; N = 20) group. Cell subsets were determined by flow cytometry. Results: Compared to ND, T2DM blood cells exhibited a higher expression of CD25+, Foxp3+, CD4+CD25+, CD4+Foxp3+ and CD25+Foxp3+; and cord blood cells showed a lower expression of CD25+, CD4+Foxp3+ and CD25+Foxp3+. In the placenta of T2DM, the villous layer of the proportion, CD3+ and CD25 was lower than that of CD4+Foxp3+ and CD25+Foxp3+, and the extravillous placenta layer contained the lowest levels of CD4+ and CD25+ and highest proportions of CD4+Foxp3+. In maternal blood from T2DM, the frequency of CD3+CD95+ and CD3CD4+ T cells expressing CD95+ was lower. In cord blood from T2DM, the rate of CD3+CD95+ was lower. The placenta villous layer of T2DM showed a lower count of CD3+CD95+ and of CD3CD4+ T cells expressing CD95+, whereas the number of cells expressing CD3+CD45RO+ decreased in both placental layers. Conclusion: The data obtained suggest that hyperglycemia changes the phenotypes of regulatory T cells and Fas expression in memory T cells. |
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Repositório Institucional da UNESP |
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Phenotypic characterization of regulatory T cells populations in maternal blood, cord blood and placenta from diabetic mothersdiabetesFasplacentapregnancyTreg cellsPurpose: Changes in regulatory T cells (Treg) in peripheral blood are associated with a number of pathologies, including diabetes. However, the immunological responses of pregnant diabetic women remain scarcely known, and the effects of Treg cells in these patients have yet to be investigated. The present study characterized the expression of regulatory T cells in the maternal blood, cord blood and placenta of diabetic pregnant women. Materials and methods: The women were divided according to glycemic status into a non-diabetic (ND; N = 20) or type 2 diabetic (T2DM; N = 20) group. Cell subsets were determined by flow cytometry. Results: Compared to ND, T2DM blood cells exhibited a higher expression of CD25+, Foxp3+, CD4+CD25+, CD4+Foxp3+ and CD25+Foxp3+; and cord blood cells showed a lower expression of CD25+, CD4+Foxp3+ and CD25+Foxp3+. In the placenta of T2DM, the villous layer of the proportion, CD3+ and CD25 was lower than that of CD4+Foxp3+ and CD25+Foxp3+, and the extravillous placenta layer contained the lowest levels of CD4+ and CD25+ and highest proportions of CD4+Foxp3+. In maternal blood from T2DM, the frequency of CD3+CD95+ and CD3CD4+ T cells expressing CD95+ was lower. In cord blood from T2DM, the rate of CD3+CD95+ was lower. The placenta villous layer of T2DM showed a lower count of CD3+CD95+ and of CD3CD4+ T cells expressing CD95+, whereas the number of cells expressing CD3+CD45RO+ decreased in both placental layers. Conclusion: The data obtained suggest that hyperglycemia changes the phenotypes of regulatory T cells and Fas expression in memory T cells.Institute of Biological and Health Science, Federal University of Mato Grosso, Barra do Garças, BrazilBotucatu Medical School, Graduate Program in Gynecology, Obstetrics and Mastology, São Paulo State University/UNESP Botucatu, BrazilUniversidade Estadual Paulista (Unesp)Queiroz, Adriele A.França, Eduardo L.Hara, Cristiane C. P.Honorio, Mariana S.Fagundes, Danny L. G.Calderon, Iracema M. P.Honorio-França, Adenilda C.2018-12-11T17:23:56Z2018-12-11T17:23:56Z2017-11-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-7http://dx.doi.org/10.1080/14767058.2017.1400004Journal of Maternal-Fetal and Neonatal Medicine, p. 1-7.1476-49541476-7058http://hdl.handle.net/11449/17708710.1080/14767058.2017.14000042-s2.0-85041374084Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Maternal-Fetal and Neonatal Medicine0,7140,714info:eu-repo/semantics/openAccess2021-10-23T16:01:08Zoai:repositorio.unesp.br:11449/177087Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T16:01:08Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Phenotypic characterization of regulatory T cells populations in maternal blood, cord blood and placenta from diabetic mothers |
title |
Phenotypic characterization of regulatory T cells populations in maternal blood, cord blood and placenta from diabetic mothers |
spellingShingle |
Phenotypic characterization of regulatory T cells populations in maternal blood, cord blood and placenta from diabetic mothers Queiroz, Adriele A. diabetes Fas placenta pregnancy Treg cells |
title_short |
Phenotypic characterization of regulatory T cells populations in maternal blood, cord blood and placenta from diabetic mothers |
title_full |
Phenotypic characterization of regulatory T cells populations in maternal blood, cord blood and placenta from diabetic mothers |
title_fullStr |
Phenotypic characterization of regulatory T cells populations in maternal blood, cord blood and placenta from diabetic mothers |
title_full_unstemmed |
Phenotypic characterization of regulatory T cells populations in maternal blood, cord blood and placenta from diabetic mothers |
title_sort |
Phenotypic characterization of regulatory T cells populations in maternal blood, cord blood and placenta from diabetic mothers |
author |
Queiroz, Adriele A. |
author_facet |
Queiroz, Adriele A. França, Eduardo L. Hara, Cristiane C. P. Honorio, Mariana S. Fagundes, Danny L. G. Calderon, Iracema M. P. Honorio-França, Adenilda C. |
author_role |
author |
author2 |
França, Eduardo L. Hara, Cristiane C. P. Honorio, Mariana S. Fagundes, Danny L. G. Calderon, Iracema M. P. Honorio-França, Adenilda C. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Queiroz, Adriele A. França, Eduardo L. Hara, Cristiane C. P. Honorio, Mariana S. Fagundes, Danny L. G. Calderon, Iracema M. P. Honorio-França, Adenilda C. |
dc.subject.por.fl_str_mv |
diabetes Fas placenta pregnancy Treg cells |
topic |
diabetes Fas placenta pregnancy Treg cells |
description |
Purpose: Changes in regulatory T cells (Treg) in peripheral blood are associated with a number of pathologies, including diabetes. However, the immunological responses of pregnant diabetic women remain scarcely known, and the effects of Treg cells in these patients have yet to be investigated. The present study characterized the expression of regulatory T cells in the maternal blood, cord blood and placenta of diabetic pregnant women. Materials and methods: The women were divided according to glycemic status into a non-diabetic (ND; N = 20) or type 2 diabetic (T2DM; N = 20) group. Cell subsets were determined by flow cytometry. Results: Compared to ND, T2DM blood cells exhibited a higher expression of CD25+, Foxp3+, CD4+CD25+, CD4+Foxp3+ and CD25+Foxp3+; and cord blood cells showed a lower expression of CD25+, CD4+Foxp3+ and CD25+Foxp3+. In the placenta of T2DM, the villous layer of the proportion, CD3+ and CD25 was lower than that of CD4+Foxp3+ and CD25+Foxp3+, and the extravillous placenta layer contained the lowest levels of CD4+ and CD25+ and highest proportions of CD4+Foxp3+. In maternal blood from T2DM, the frequency of CD3+CD95+ and CD3CD4+ T cells expressing CD95+ was lower. In cord blood from T2DM, the rate of CD3+CD95+ was lower. The placenta villous layer of T2DM showed a lower count of CD3+CD95+ and of CD3CD4+ T cells expressing CD95+, whereas the number of cells expressing CD3+CD45RO+ decreased in both placental layers. Conclusion: The data obtained suggest that hyperglycemia changes the phenotypes of regulatory T cells and Fas expression in memory T cells. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-11-15 2018-12-11T17:23:56Z 2018-12-11T17:23:56Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1080/14767058.2017.1400004 Journal of Maternal-Fetal and Neonatal Medicine, p. 1-7. 1476-4954 1476-7058 http://hdl.handle.net/11449/177087 10.1080/14767058.2017.1400004 2-s2.0-85041374084 |
url |
http://dx.doi.org/10.1080/14767058.2017.1400004 http://hdl.handle.net/11449/177087 |
identifier_str_mv |
Journal of Maternal-Fetal and Neonatal Medicine, p. 1-7. 1476-4954 1476-7058 10.1080/14767058.2017.1400004 2-s2.0-85041374084 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Maternal-Fetal and Neonatal Medicine 0,714 0,714 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1-7 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965265129111552 |