The exposure in ovo of embryos belonging to Amazonian turtle species Podocnemis expansa (Testudines) to commercial glyphosate and fipronil formulations impairs their growth and changes their skeletal development
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.scitotenv.2022.156709 http://hdl.handle.net/11449/240327 |
Resumo: | Pesticides are widely used in agricultural production; moreover, they can have direct and indirect effect on both flora and fauna. Aquatic organisms, among other animals, including reptiles, are mainly susceptible to contamination effects. Accordingly, the aim of the present study is to test the hypothesis that the incubation of Podocnemis expansa eggs in substrate added with glyphosate and fipronil formulations changes their viability, interferes with their growth and induces bone alterations. Eggs collected in natural environment were artificially incubated in sand moistened with water added with glyphosate Atar 48, at concentrations of 65 or 6500 μg/L (groups G1 and G2, respectively), and with fipronil Regent 800 WG at 4 or 400 μg/L (groups F1 and F2, respectively) or, yet, with the combination of 65 μg/L glyphosate and 4 μg/L fipronil, or with 6500 μg/L glyphosate and 400 μg/L fipronil (groups GF1 and GF2, respectively). The level of exposure to the herein assessed pesticides was quantified at the end of the incubation period; it was done by dosing its concentration in eggshells. Eggs exposed to the tested pesticides did not have their viability affected by it; however, all embryos exposed to the tested pesticides showed lowered body mass at hatch, as well as impaired development. In addition, bone malformation in the scleral ossicular ring was observed in individuals in groups F1, F2 and GF1. Pesticides accumulated in eggshells at concentrations related to exposure level. Thus, the recorded results have evidenced some remarkably relevant, and previously unknown, impacts associated with the exposure of a species listed as lower risk/conservation dependent, which spends most of its life in the water, to two widely used pesticides, at a very sensitive stage of its life, namely: egg incubation on land. |
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The exposure in ovo of embryos belonging to Amazonian turtle species Podocnemis expansa (Testudines) to commercial glyphosate and fipronil formulations impairs their growth and changes their skeletal developmentEcotoxicologyMalformationsOntogenyPesticidesReptilesPesticides are widely used in agricultural production; moreover, they can have direct and indirect effect on both flora and fauna. Aquatic organisms, among other animals, including reptiles, are mainly susceptible to contamination effects. Accordingly, the aim of the present study is to test the hypothesis that the incubation of Podocnemis expansa eggs in substrate added with glyphosate and fipronil formulations changes their viability, interferes with their growth and induces bone alterations. Eggs collected in natural environment were artificially incubated in sand moistened with water added with glyphosate Atar 48, at concentrations of 65 or 6500 μg/L (groups G1 and G2, respectively), and with fipronil Regent 800 WG at 4 or 400 μg/L (groups F1 and F2, respectively) or, yet, with the combination of 65 μg/L glyphosate and 4 μg/L fipronil, or with 6500 μg/L glyphosate and 400 μg/L fipronil (groups GF1 and GF2, respectively). The level of exposure to the herein assessed pesticides was quantified at the end of the incubation period; it was done by dosing its concentration in eggshells. Eggs exposed to the tested pesticides did not have their viability affected by it; however, all embryos exposed to the tested pesticides showed lowered body mass at hatch, as well as impaired development. In addition, bone malformation in the scleral ossicular ring was observed in individuals in groups F1, F2 and GF1. Pesticides accumulated in eggshells at concentrations related to exposure level. Thus, the recorded results have evidenced some remarkably relevant, and previously unknown, impacts associated with the exposure of a species listed as lower risk/conservation dependent, which spends most of its life in the water, to two widely used pesticides, at a very sensitive stage of its life, namely: egg incubation on land.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Programa de Pós-Graduação em Ecologia Evolução e Biodiversidade Instituto de Biociências Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), SPFaculdade de Agronomia e Medicina Veterinária Universidade de Brasília (UNB), DFLaboratório de Ensino e Pesquisa em Animais Silvestres Universidade Federal de Uberlândia (UFU), MGLaboratório de Genética e Mutagênese Instituto de Ciências Biológicas Universidade Federal de Goiás (UFG), GOLaboratório Multidisciplinar em Morfologia e Ontogenia Instituto de Ciências Biológicas Universidade Federal de Goiás (UFG), GOLaboratório de Toxicologia Amplicada ao Meio Ambiente Programa de Pós-Graduação em Conservação dos Recursos Naturais do Cerrado Instituto Federal Goiano – Campus Urutaí, GOPrograma de Pós-Graduação em Ciências Ambientais Universidade Federal de Goiás (UFG), GOPrograma de Pós-Graduação em Biotecnologia e Biodiversidade Instituição Federal Goiano e Universidade Federal de Goiás, GOPrograma de Pós-Graduação em Ecologia Conservação e Biodiversidade Universidade Federal de Uberlândia (UFU), MGInstituto de Ciências Exatas e Naturais do Pontal Universidade Federal de Uberlândia (UFU), MGDepartamento de Biodiversidade Instituto de Biociências Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), SPPrograma de Pós-Graduação em Ecologia Evolução e Biodiversidade Instituto de Biociências Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), SPDepartamento de Biodiversidade Instituto de Biociências Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), SPCAPES: 001FAPESP: 13/04190-9FAPESP: 17/17615-9FAPESP: 18/05839-2CNPq: 302227/2019-9CNPq: 306811/2015-4Universidade Estadual Paulista (UNESP)Universidade de Brasília (UnB)Universidade Federal de Uberlândia (UFU)Universidade Federal de Goiás (UFG)Instituto Federal Goiano – Campus UrutaíMendonça, Juliana dos Santos [UNESP]Hirano, Líria Queiroz LuzSantos, André Luiz QuagliattoSilva, Daniela de Melo ePereira, Carolina de CastroCosta, Matheus SantosLima, Phâmella Neres deAraújo, Amanda Pereira da CostaMalafaia, GuilhermeAmbrósio, Isabela SayuriCastilho, Lais de Moura BifeSantos, Allyson Leandro Rodrigues dosFaria, Anizio Marcio deAndrade, Denis Vieira de [UNESP]Vieira, Lucélia Gonçalves2023-03-01T20:12:04Z2023-03-01T20:12:04Z2022-10-10info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.scitotenv.2022.156709Science of the Total Environment, v. 842.1879-10260048-9697http://hdl.handle.net/11449/24032710.1016/j.scitotenv.2022.1567092-s2.0-85132719150Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengScience of the Total Environmentinfo:eu-repo/semantics/openAccess2023-03-01T20:12:04Zoai:repositorio.unesp.br:11449/240327Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-03-01T20:12:04Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
The exposure in ovo of embryos belonging to Amazonian turtle species Podocnemis expansa (Testudines) to commercial glyphosate and fipronil formulations impairs their growth and changes their skeletal development |
title |
The exposure in ovo of embryos belonging to Amazonian turtle species Podocnemis expansa (Testudines) to commercial glyphosate and fipronil formulations impairs their growth and changes their skeletal development |
spellingShingle |
The exposure in ovo of embryos belonging to Amazonian turtle species Podocnemis expansa (Testudines) to commercial glyphosate and fipronil formulations impairs their growth and changes their skeletal development Mendonça, Juliana dos Santos [UNESP] Ecotoxicology Malformations Ontogeny Pesticides Reptiles |
title_short |
The exposure in ovo of embryos belonging to Amazonian turtle species Podocnemis expansa (Testudines) to commercial glyphosate and fipronil formulations impairs their growth and changes their skeletal development |
title_full |
The exposure in ovo of embryos belonging to Amazonian turtle species Podocnemis expansa (Testudines) to commercial glyphosate and fipronil formulations impairs their growth and changes their skeletal development |
title_fullStr |
The exposure in ovo of embryos belonging to Amazonian turtle species Podocnemis expansa (Testudines) to commercial glyphosate and fipronil formulations impairs their growth and changes their skeletal development |
title_full_unstemmed |
The exposure in ovo of embryos belonging to Amazonian turtle species Podocnemis expansa (Testudines) to commercial glyphosate and fipronil formulations impairs their growth and changes their skeletal development |
title_sort |
The exposure in ovo of embryos belonging to Amazonian turtle species Podocnemis expansa (Testudines) to commercial glyphosate and fipronil formulations impairs their growth and changes their skeletal development |
author |
Mendonça, Juliana dos Santos [UNESP] |
author_facet |
Mendonça, Juliana dos Santos [UNESP] Hirano, Líria Queiroz Luz Santos, André Luiz Quagliatto Silva, Daniela de Melo e Pereira, Carolina de Castro Costa, Matheus Santos Lima, Phâmella Neres de Araújo, Amanda Pereira da Costa Malafaia, Guilherme Ambrósio, Isabela Sayuri Castilho, Lais de Moura Bife Santos, Allyson Leandro Rodrigues dos Faria, Anizio Marcio de Andrade, Denis Vieira de [UNESP] Vieira, Lucélia Gonçalves |
author_role |
author |
author2 |
Hirano, Líria Queiroz Luz Santos, André Luiz Quagliatto Silva, Daniela de Melo e Pereira, Carolina de Castro Costa, Matheus Santos Lima, Phâmella Neres de Araújo, Amanda Pereira da Costa Malafaia, Guilherme Ambrósio, Isabela Sayuri Castilho, Lais de Moura Bife Santos, Allyson Leandro Rodrigues dos Faria, Anizio Marcio de Andrade, Denis Vieira de [UNESP] Vieira, Lucélia Gonçalves |
author2_role |
author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade de Brasília (UnB) Universidade Federal de Uberlândia (UFU) Universidade Federal de Goiás (UFG) Instituto Federal Goiano – Campus Urutaí |
dc.contributor.author.fl_str_mv |
Mendonça, Juliana dos Santos [UNESP] Hirano, Líria Queiroz Luz Santos, André Luiz Quagliatto Silva, Daniela de Melo e Pereira, Carolina de Castro Costa, Matheus Santos Lima, Phâmella Neres de Araújo, Amanda Pereira da Costa Malafaia, Guilherme Ambrósio, Isabela Sayuri Castilho, Lais de Moura Bife Santos, Allyson Leandro Rodrigues dos Faria, Anizio Marcio de Andrade, Denis Vieira de [UNESP] Vieira, Lucélia Gonçalves |
dc.subject.por.fl_str_mv |
Ecotoxicology Malformations Ontogeny Pesticides Reptiles |
topic |
Ecotoxicology Malformations Ontogeny Pesticides Reptiles |
description |
Pesticides are widely used in agricultural production; moreover, they can have direct and indirect effect on both flora and fauna. Aquatic organisms, among other animals, including reptiles, are mainly susceptible to contamination effects. Accordingly, the aim of the present study is to test the hypothesis that the incubation of Podocnemis expansa eggs in substrate added with glyphosate and fipronil formulations changes their viability, interferes with their growth and induces bone alterations. Eggs collected in natural environment were artificially incubated in sand moistened with water added with glyphosate Atar 48, at concentrations of 65 or 6500 μg/L (groups G1 and G2, respectively), and with fipronil Regent 800 WG at 4 or 400 μg/L (groups F1 and F2, respectively) or, yet, with the combination of 65 μg/L glyphosate and 4 μg/L fipronil, or with 6500 μg/L glyphosate and 400 μg/L fipronil (groups GF1 and GF2, respectively). The level of exposure to the herein assessed pesticides was quantified at the end of the incubation period; it was done by dosing its concentration in eggshells. Eggs exposed to the tested pesticides did not have their viability affected by it; however, all embryos exposed to the tested pesticides showed lowered body mass at hatch, as well as impaired development. In addition, bone malformation in the scleral ossicular ring was observed in individuals in groups F1, F2 and GF1. Pesticides accumulated in eggshells at concentrations related to exposure level. Thus, the recorded results have evidenced some remarkably relevant, and previously unknown, impacts associated with the exposure of a species listed as lower risk/conservation dependent, which spends most of its life in the water, to two widely used pesticides, at a very sensitive stage of its life, namely: egg incubation on land. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-10-10 2023-03-01T20:12:04Z 2023-03-01T20:12:04Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.scitotenv.2022.156709 Science of the Total Environment, v. 842. 1879-1026 0048-9697 http://hdl.handle.net/11449/240327 10.1016/j.scitotenv.2022.156709 2-s2.0-85132719150 |
url |
http://dx.doi.org/10.1016/j.scitotenv.2022.156709 http://hdl.handle.net/11449/240327 |
identifier_str_mv |
Science of the Total Environment, v. 842. 1879-1026 0048-9697 10.1016/j.scitotenv.2022.156709 2-s2.0-85132719150 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Science of the Total Environment |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1792961918932090880 |