Effects of modified Portland cement and MTA on fibroblast viability and cytokine production
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/232412 |
Resumo: | Objective: The aim of this study was to investigate the effects of a new Portland Cement formulation (CPM) comparing it to Angelus MTA on cell viability and IL-1β and IL-6 release by mouse fibroblasts. Methods: Polyethylene tubes filled with these materials were placed into 24-well cell culture plates with mouse fibroblasts. Empty tubes were used as control. After 24 hours, MTT assay was used to evaluate the cell viability. For cytokine assay, mouse fibroblasts were incubated in 24-well flat-bottom plates with set material disks at the bottom or without material, as control. After 24 hours, culture media were collected for cytokine evaluation by using ELISA. Results: CPM and Angelus MTA did not inhibit the cell viability. Both materials induced IL-6 and IL-1β release and the amount was statistically significant compared with the control group. Conclusions: Both materials were not cytotoxic in fibroblast culture and induced IL-6 and IL-1β release. |
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Repositório Institucional da UNESP |
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spelling |
Effects of modified Portland cement and MTA on fibroblast viability and cytokine productionCytotoxicityDental materialsMTAObjective: The aim of this study was to investigate the effects of a new Portland Cement formulation (CPM) comparing it to Angelus MTA on cell viability and IL-1β and IL-6 release by mouse fibroblasts. Methods: Polyethylene tubes filled with these materials were placed into 24-well cell culture plates with mouse fibroblasts. Empty tubes were used as control. After 24 hours, MTT assay was used to evaluate the cell viability. For cytokine assay, mouse fibroblasts were incubated in 24-well flat-bottom plates with set material disks at the bottom or without material, as control. After 24 hours, culture media were collected for cytokine evaluation by using ELISA. Results: CPM and Angelus MTA did not inhibit the cell viability. Both materials induced IL-6 and IL-1β release and the amount was statistically significant compared with the control group. Conclusions: Both materials were not cytotoxic in fibroblast culture and induced IL-6 and IL-1β release.FOA-UNESPFOA-UNESPUniversidade Estadual Paulista (UNESP)Gomes Filho, João Eduardo [UNESP]Cintra, Luciano Tavares Angelo [UNESP]Dezan Junior, Eloi [UNESP]Watanabe, Simone [UNESP]Faria, Max Douglas [UNESP]Gomes, Alessandra Cristina [UNESP]Lodi, Carolina Simonetti [UNESP]Oliveira, Sandra Helena Penha de [UNESP]2022-04-29T15:27:03Z2022-04-29T15:27:03Z2012-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article20-24Dental Press Endodontics, v. 2, n. 2, p. 20-24, 2012.2178-3713http://hdl.handle.net/11449/2324122-s2.0-84930918005Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengDental Press Endodonticsinfo:eu-repo/semantics/openAccess2023-02-06T14:43:06Zoai:repositorio.unesp.br:11449/232412Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-02-06T14:43:06Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effects of modified Portland cement and MTA on fibroblast viability and cytokine production |
title |
Effects of modified Portland cement and MTA on fibroblast viability and cytokine production |
spellingShingle |
Effects of modified Portland cement and MTA on fibroblast viability and cytokine production Gomes Filho, João Eduardo [UNESP] Cytotoxicity Dental materials MTA |
title_short |
Effects of modified Portland cement and MTA on fibroblast viability and cytokine production |
title_full |
Effects of modified Portland cement and MTA on fibroblast viability and cytokine production |
title_fullStr |
Effects of modified Portland cement and MTA on fibroblast viability and cytokine production |
title_full_unstemmed |
Effects of modified Portland cement and MTA on fibroblast viability and cytokine production |
title_sort |
Effects of modified Portland cement and MTA on fibroblast viability and cytokine production |
author |
Gomes Filho, João Eduardo [UNESP] |
author_facet |
Gomes Filho, João Eduardo [UNESP] Cintra, Luciano Tavares Angelo [UNESP] Dezan Junior, Eloi [UNESP] Watanabe, Simone [UNESP] Faria, Max Douglas [UNESP] Gomes, Alessandra Cristina [UNESP] Lodi, Carolina Simonetti [UNESP] Oliveira, Sandra Helena Penha de [UNESP] |
author_role |
author |
author2 |
Cintra, Luciano Tavares Angelo [UNESP] Dezan Junior, Eloi [UNESP] Watanabe, Simone [UNESP] Faria, Max Douglas [UNESP] Gomes, Alessandra Cristina [UNESP] Lodi, Carolina Simonetti [UNESP] Oliveira, Sandra Helena Penha de [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Gomes Filho, João Eduardo [UNESP] Cintra, Luciano Tavares Angelo [UNESP] Dezan Junior, Eloi [UNESP] Watanabe, Simone [UNESP] Faria, Max Douglas [UNESP] Gomes, Alessandra Cristina [UNESP] Lodi, Carolina Simonetti [UNESP] Oliveira, Sandra Helena Penha de [UNESP] |
dc.subject.por.fl_str_mv |
Cytotoxicity Dental materials MTA |
topic |
Cytotoxicity Dental materials MTA |
description |
Objective: The aim of this study was to investigate the effects of a new Portland Cement formulation (CPM) comparing it to Angelus MTA on cell viability and IL-1β and IL-6 release by mouse fibroblasts. Methods: Polyethylene tubes filled with these materials were placed into 24-well cell culture plates with mouse fibroblasts. Empty tubes were used as control. After 24 hours, MTT assay was used to evaluate the cell viability. For cytokine assay, mouse fibroblasts were incubated in 24-well flat-bottom plates with set material disks at the bottom or without material, as control. After 24 hours, culture media were collected for cytokine evaluation by using ELISA. Results: CPM and Angelus MTA did not inhibit the cell viability. Both materials induced IL-6 and IL-1β release and the amount was statistically significant compared with the control group. Conclusions: Both materials were not cytotoxic in fibroblast culture and induced IL-6 and IL-1β release. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-01-01 2022-04-29T15:27:03Z 2022-04-29T15:27:03Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Dental Press Endodontics, v. 2, n. 2, p. 20-24, 2012. 2178-3713 http://hdl.handle.net/11449/232412 2-s2.0-84930918005 |
identifier_str_mv |
Dental Press Endodontics, v. 2, n. 2, p. 20-24, 2012. 2178-3713 2-s2.0-84930918005 |
url |
http://hdl.handle.net/11449/232412 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Dental Press Endodontics |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
20-24 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797789624212389888 |