Low Mg content on Ti-Nb-Sn alloy when in contact with eBMMSCs promotes improvement of its biological functions
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Publication Date: | 2021 |
Other Authors: | , , , , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Institucional da UNESP |
Download full: | http://dx.doi.org/10.1007/s10856-021-06620-9 http://hdl.handle.net/11449/233879 |
Summary: | Magnesium is a metal used in the composition of titanium alloys and imparts porosity. Due to its osteoconductive, biocompatible and biodegradable characteristics, its application in the development of biomedical materials has become attractive. This study aimed to evaluate the influence of magnesium present in porous Ti-Nb-Sn alloys, which have a low elastic modulus in adhesive, osteogenic properties and the amount of reactive intracellular oxygen species released in mesenchymal stem cells derived from bone marrow equine bone (eBMMSCs). Mechanical properties of the alloy, such as hardness, compressive strength and elastic modulus, were analyzed, as well as surface morphological characteristics through scanning electron microscopy. The evaluation of magnesium ion release was performed by atomic force spectroscopy. The biological characteristics of the alloy, when in contact with the alloy surface and with the culture medium conditioned with the alloy, were studied by SEM and optical microscopy. Confirmation of osteogenic differentiation by alizarin red and detection of ROS using a Muse® Oxidative Stress Kit based on dihydroetide (DHE). The alloy showed an elastic modulus close to cortical bone values. The hardness was close to commercial Ti grade 2, and the compressive strength was greater than the value of cortical bone. The eBMMSCs adhered to the surface of the alloy during the experimental time. Osteogenic differentiation was observed with the treatment of eBMMMSCs with conditioned medium. The eBMMSCs treated with conditioned medium decreased ROS production, indicating a possible antioxidant defense potential of magnesium release. [Figure not available: see fulltext.] |
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Low Mg content on Ti-Nb-Sn alloy when in contact with eBMMSCs promotes improvement of its biological functionsMagnesium is a metal used in the composition of titanium alloys and imparts porosity. Due to its osteoconductive, biocompatible and biodegradable characteristics, its application in the development of biomedical materials has become attractive. This study aimed to evaluate the influence of magnesium present in porous Ti-Nb-Sn alloys, which have a low elastic modulus in adhesive, osteogenic properties and the amount of reactive intracellular oxygen species released in mesenchymal stem cells derived from bone marrow equine bone (eBMMSCs). Mechanical properties of the alloy, such as hardness, compressive strength and elastic modulus, were analyzed, as well as surface morphological characteristics through scanning electron microscopy. The evaluation of magnesium ion release was performed by atomic force spectroscopy. The biological characteristics of the alloy, when in contact with the alloy surface and with the culture medium conditioned with the alloy, were studied by SEM and optical microscopy. Confirmation of osteogenic differentiation by alizarin red and detection of ROS using a Muse® Oxidative Stress Kit based on dihydroetide (DHE). The alloy showed an elastic modulus close to cortical bone values. The hardness was close to commercial Ti grade 2, and the compressive strength was greater than the value of cortical bone. The eBMMSCs adhered to the surface of the alloy during the experimental time. Osteogenic differentiation was observed with the treatment of eBMMMSCs with conditioned medium. The eBMMSCs treated with conditioned medium decreased ROS production, indicating a possible antioxidant defense potential of magnesium release. [Figure not available: see fulltext.]Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Veterinary Surgery and Anesthesiology São Paulo State University - Júlio de Mesquita Filho Unesp Prof. Doutor Walter Mauricio Correa St. n/nDepartment of Veterinary Surgery and Anesthesiology São Paulo State University - Júlio de Mesquita Filho Unesp Prof. Doutor Walter Mauricio Correa St. n/nFAPESP: 2019/09752-1Universidade Estadual Paulista (UNESP)da Silva Dias, Carolina [UNESP]Rossi, Mariana Correa [UNESP]Apolonio, Emanuel V. P. [UNESP]dos Santos Rosa, Gustavo [UNESP]Pfeifer, João Pedro Hübbe [UNESP]Hussni, Carlos Alberto [UNESP]Watanabe, Marcos Jun [UNESP]Alves, Ana Liz Garcia [UNESP]2022-05-01T11:07:38Z2022-05-01T11:07:38Z2021-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s10856-021-06620-9Journal of Materials Science: Materials in Medicine, v. 32, n. 12, 2021.1573-48380957-4530http://hdl.handle.net/11449/23387910.1007/s10856-021-06620-92-s2.0-85120740776Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Materials Science: Materials in Medicineinfo:eu-repo/semantics/openAccess2022-05-01T11:07:38Zoai:repositorio.unesp.br:11449/233879Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-05-01T11:07:38Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Low Mg content on Ti-Nb-Sn alloy when in contact with eBMMSCs promotes improvement of its biological functions |
title |
Low Mg content on Ti-Nb-Sn alloy when in contact with eBMMSCs promotes improvement of its biological functions |
spellingShingle |
Low Mg content on Ti-Nb-Sn alloy when in contact with eBMMSCs promotes improvement of its biological functions da Silva Dias, Carolina [UNESP] |
title_short |
Low Mg content on Ti-Nb-Sn alloy when in contact with eBMMSCs promotes improvement of its biological functions |
title_full |
Low Mg content on Ti-Nb-Sn alloy when in contact with eBMMSCs promotes improvement of its biological functions |
title_fullStr |
Low Mg content on Ti-Nb-Sn alloy when in contact with eBMMSCs promotes improvement of its biological functions |
title_full_unstemmed |
Low Mg content on Ti-Nb-Sn alloy when in contact with eBMMSCs promotes improvement of its biological functions |
title_sort |
Low Mg content on Ti-Nb-Sn alloy when in contact with eBMMSCs promotes improvement of its biological functions |
author |
da Silva Dias, Carolina [UNESP] |
author_facet |
da Silva Dias, Carolina [UNESP] Rossi, Mariana Correa [UNESP] Apolonio, Emanuel V. P. [UNESP] dos Santos Rosa, Gustavo [UNESP] Pfeifer, João Pedro Hübbe [UNESP] Hussni, Carlos Alberto [UNESP] Watanabe, Marcos Jun [UNESP] Alves, Ana Liz Garcia [UNESP] |
author_role |
author |
author2 |
Rossi, Mariana Correa [UNESP] Apolonio, Emanuel V. P. [UNESP] dos Santos Rosa, Gustavo [UNESP] Pfeifer, João Pedro Hübbe [UNESP] Hussni, Carlos Alberto [UNESP] Watanabe, Marcos Jun [UNESP] Alves, Ana Liz Garcia [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
da Silva Dias, Carolina [UNESP] Rossi, Mariana Correa [UNESP] Apolonio, Emanuel V. P. [UNESP] dos Santos Rosa, Gustavo [UNESP] Pfeifer, João Pedro Hübbe [UNESP] Hussni, Carlos Alberto [UNESP] Watanabe, Marcos Jun [UNESP] Alves, Ana Liz Garcia [UNESP] |
description |
Magnesium is a metal used in the composition of titanium alloys and imparts porosity. Due to its osteoconductive, biocompatible and biodegradable characteristics, its application in the development of biomedical materials has become attractive. This study aimed to evaluate the influence of magnesium present in porous Ti-Nb-Sn alloys, which have a low elastic modulus in adhesive, osteogenic properties and the amount of reactive intracellular oxygen species released in mesenchymal stem cells derived from bone marrow equine bone (eBMMSCs). Mechanical properties of the alloy, such as hardness, compressive strength and elastic modulus, were analyzed, as well as surface morphological characteristics through scanning electron microscopy. The evaluation of magnesium ion release was performed by atomic force spectroscopy. The biological characteristics of the alloy, when in contact with the alloy surface and with the culture medium conditioned with the alloy, were studied by SEM and optical microscopy. Confirmation of osteogenic differentiation by alizarin red and detection of ROS using a Muse® Oxidative Stress Kit based on dihydroetide (DHE). The alloy showed an elastic modulus close to cortical bone values. The hardness was close to commercial Ti grade 2, and the compressive strength was greater than the value of cortical bone. The eBMMSCs adhered to the surface of the alloy during the experimental time. Osteogenic differentiation was observed with the treatment of eBMMMSCs with conditioned medium. The eBMMSCs treated with conditioned medium decreased ROS production, indicating a possible antioxidant defense potential of magnesium release. [Figure not available: see fulltext.] |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-12-01 2022-05-01T11:07:38Z 2022-05-01T11:07:38Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s10856-021-06620-9 Journal of Materials Science: Materials in Medicine, v. 32, n. 12, 2021. 1573-4838 0957-4530 http://hdl.handle.net/11449/233879 10.1007/s10856-021-06620-9 2-s2.0-85120740776 |
url |
http://dx.doi.org/10.1007/s10856-021-06620-9 http://hdl.handle.net/11449/233879 |
identifier_str_mv |
Journal of Materials Science: Materials in Medicine, v. 32, n. 12, 2021. 1573-4838 0957-4530 10.1007/s10856-021-06620-9 2-s2.0-85120740776 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Materials Science: Materials in Medicine |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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