Bovine herpesvirus-5 infection in a rabbit experimental model: Immunohistochemical study of the cellular response in the CNS
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.micpath.2013.01.003 http://hdl.handle.net/11449/74935 |
Resumo: | Since little information is available regarding cellular antigen mapping and the involvement of non-neuronal cells in the pathogenesis of bovine herpesvirus type 5 (BHV-5) infection, it were determined the BHV-5 distribution, the astrocytic reactivity, the involvement of lymphocytes and the presence of matrix metalloproteinase (MMP)-9 in the brain of rabbits experimentally infected with BHV-5. Twelve New Zealand rabbits that were seronegative for BHV-5 were used for virus inoculation, and five rabbits were used as mock-infected controls. The rabbits were kept in separate areas and were inoculated intranasally with 500 μl of virus suspension (EVI 88 Brazilian isolate) into each nostril (virus titer, 107.5 TCID50). Control rabbits were inoculated with the same volume of minimum essential medium. Five days before virus inoculation, the rabbits were submitted to daily administration of dexamethasone. After virus inoculation, the rabbits were monitored clinically on a daily basis. Seven rabbits showed respiratory symptoms and four animals exhibited neurological symptoms. Tissue sections were collected for histological examination and immunohistochemistry to examine BHV-5 antigens, astrocytes, T and B lymphocytes and MMP-9. By means of immunohistochemical and PCR methods, BHV-5 was detected in the entire brain of the animals which presented with neurological symptoms, especially in the trigeminal ganglion and cerebral cortices. Furthermore, BHV-5 antigens were detected in neurons and/or other non-neural cells. In addition to the neurons, most infiltrating CD3 T lymphocytes observed in these areas were positive for MMP-9 and also for BHV-5 antigen. These infected cells might contribute to the spread of the virus to the rabbit brain along the trigeminal ganglia and olfactory nerve pathways. © 2013 Elsevier Ltd. |
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Bovine herpesvirus-5 infection in a rabbit experimental model: Immunohistochemical study of the cellular response in the CNSAnimal modelAstrocytesInflammationLymphocytesMatrix metalloproteinasesBovine herpespirus 5 antigencell antigendexamethasonegelatinase Bunclassified drugvirus antigenanimal cellanimal experimentanimal modelanimal tissueantibody titerastrocyteB lymphocyteBovine herpes virusbrainbrain cortexCD3+ T lymphocytecellular immunitycentral nervous systemcontrolled studyenzyme activityexperimental modelherpes virus infectionimmunohistochemistryimmunopathogenesisinoculationlymphocytelymphocytic infiltrationmalenerve cellneurologic diseasenonhumannosepriority journalrabbitrespiratory tract diseasesuspension cell culturesymptomatologyT lymphocytetrigeminus ganglionAnimalsBrainCattleDisease Models, AnimalEncephalitis, ViralHerpesviridae InfectionsHerpesvirus 5, BovineMaleMatrix Metalloproteinase 9MeningoencephalitisRabbitsSince little information is available regarding cellular antigen mapping and the involvement of non-neuronal cells in the pathogenesis of bovine herpesvirus type 5 (BHV-5) infection, it were determined the BHV-5 distribution, the astrocytic reactivity, the involvement of lymphocytes and the presence of matrix metalloproteinase (MMP)-9 in the brain of rabbits experimentally infected with BHV-5. Twelve New Zealand rabbits that were seronegative for BHV-5 were used for virus inoculation, and five rabbits were used as mock-infected controls. The rabbits were kept in separate areas and were inoculated intranasally with 500 μl of virus suspension (EVI 88 Brazilian isolate) into each nostril (virus titer, 107.5 TCID50). Control rabbits were inoculated with the same volume of minimum essential medium. Five days before virus inoculation, the rabbits were submitted to daily administration of dexamethasone. After virus inoculation, the rabbits were monitored clinically on a daily basis. Seven rabbits showed respiratory symptoms and four animals exhibited neurological symptoms. Tissue sections were collected for histological examination and immunohistochemistry to examine BHV-5 antigens, astrocytes, T and B lymphocytes and MMP-9. By means of immunohistochemical and PCR methods, BHV-5 was detected in the entire brain of the animals which presented with neurological symptoms, especially in the trigeminal ganglion and cerebral cortices. Furthermore, BHV-5 antigens were detected in neurons and/or other non-neural cells. In addition to the neurons, most infiltrating CD3 T lymphocytes observed in these areas were positive for MMP-9 and also for BHV-5 antigen. These infected cells might contribute to the spread of the virus to the rabbit brain along the trigeminal ganglia and olfactory nerve pathways. © 2013 Elsevier Ltd.Universidade Estadual Paulista (UNESP) Faculdade de Medicina Veterinária Departamento de Clínica, Cirurgia e Reprodução Animal, Araçatuba, SPUniv de São Paulo Faculdade de Medicina Veterinária e Zootecnia Departamento de Patologia, São Paulo, SPUniv Federal de Santa Maria Departamento de Medicina Veterinária Preventiva Departamento de Microbiologia e Parasitologia, Santa Maria, RSUniv Federal do Rio Grande do Sul Instituto de Ciências Básicas da Saúde Departamento de Microbiologia, Porto Alegre, RSUniversidade Estadual Paulista (UNESP) Faculdade de Ciências Agrárias e Veterinárias Departamento de Patologia Veterinária, Jaboticabal, SPUniv Federal de Santa Catarina Curso de Medicina Veterinária, Curitibanos, SCUniversidade Estadual Paulista (UNESP) Faculdade de Medicina Veterinária Departamento de Clínica, Cirurgia e Reprodução Animal, Araçatuba, SPUniversidade Estadual Paulista (UNESP) Faculdade de Ciências Agrárias e Veterinárias Departamento de Patologia Veterinária, Jaboticabal, SPUniversidade Estadual Paulista (Unesp)Faculdade de Medicina Veterinária e ZootecniaDepartamento de Medicina Veterinária PreventivaInstituto de Ciências Básicas da SaúdeCurso de Medicina VeterináriaMachado, Gisele Fabrino [UNESP]Bernardi, FernandaHosomi, Fernando Y.M.Peiró, Juliana Regina [UNESP]Weiblen, RudiRoehe, Paulo M.Alessi, Antonio C. [UNESP]Melo, Guilherme D. [UNESP]Ramos, Adriano T.Maiorka, Paulo C.2014-05-27T11:28:45Z2014-05-27T11:28:45Z2013-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10-16application/pdfhttp://dx.doi.org/10.1016/j.micpath.2013.01.003Microbial Pathogenesis, v. 57, p. 10-16.0882-40101096-1208http://hdl.handle.net/11449/7493510.1016/j.micpath.2013.01.003WOS:0003169214000022-s2.0-848739686052-s2.0-84873968605.pdf03104055581256342976129874554698Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMicrobial Pathogenesis2.3320,7510,751info:eu-repo/semantics/openAccess2024-01-02T06:21:09Zoai:repositorio.unesp.br:11449/74935Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-02T06:21:09Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Bovine herpesvirus-5 infection in a rabbit experimental model: Immunohistochemical study of the cellular response in the CNS |
title |
Bovine herpesvirus-5 infection in a rabbit experimental model: Immunohistochemical study of the cellular response in the CNS |
spellingShingle |
Bovine herpesvirus-5 infection in a rabbit experimental model: Immunohistochemical study of the cellular response in the CNS Machado, Gisele Fabrino [UNESP] Animal model Astrocytes Inflammation Lymphocytes Matrix metalloproteinases Bovine herpespirus 5 antigen cell antigen dexamethasone gelatinase B unclassified drug virus antigen animal cell animal experiment animal model animal tissue antibody titer astrocyte B lymphocyte Bovine herpes virus brain brain cortex CD3+ T lymphocyte cellular immunity central nervous system controlled study enzyme activity experimental model herpes virus infection immunohistochemistry immunopathogenesis inoculation lymphocyte lymphocytic infiltration male nerve cell neurologic disease nonhuman nose priority journal rabbit respiratory tract disease suspension cell culture symptomatology T lymphocyte trigeminus ganglion Animals Brain Cattle Disease Models, Animal Encephalitis, Viral Herpesviridae Infections Herpesvirus 5, Bovine Male Matrix Metalloproteinase 9 Meningoencephalitis Rabbits |
title_short |
Bovine herpesvirus-5 infection in a rabbit experimental model: Immunohistochemical study of the cellular response in the CNS |
title_full |
Bovine herpesvirus-5 infection in a rabbit experimental model: Immunohistochemical study of the cellular response in the CNS |
title_fullStr |
Bovine herpesvirus-5 infection in a rabbit experimental model: Immunohistochemical study of the cellular response in the CNS |
title_full_unstemmed |
Bovine herpesvirus-5 infection in a rabbit experimental model: Immunohistochemical study of the cellular response in the CNS |
title_sort |
Bovine herpesvirus-5 infection in a rabbit experimental model: Immunohistochemical study of the cellular response in the CNS |
author |
Machado, Gisele Fabrino [UNESP] |
author_facet |
Machado, Gisele Fabrino [UNESP] Bernardi, Fernanda Hosomi, Fernando Y.M. Peiró, Juliana Regina [UNESP] Weiblen, Rudi Roehe, Paulo M. Alessi, Antonio C. [UNESP] Melo, Guilherme D. [UNESP] Ramos, Adriano T. Maiorka, Paulo C. |
author_role |
author |
author2 |
Bernardi, Fernanda Hosomi, Fernando Y.M. Peiró, Juliana Regina [UNESP] Weiblen, Rudi Roehe, Paulo M. Alessi, Antonio C. [UNESP] Melo, Guilherme D. [UNESP] Ramos, Adriano T. Maiorka, Paulo C. |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Faculdade de Medicina Veterinária e Zootecnia Departamento de Medicina Veterinária Preventiva Instituto de Ciências Básicas da Saúde Curso de Medicina Veterinária |
dc.contributor.author.fl_str_mv |
Machado, Gisele Fabrino [UNESP] Bernardi, Fernanda Hosomi, Fernando Y.M. Peiró, Juliana Regina [UNESP] Weiblen, Rudi Roehe, Paulo M. Alessi, Antonio C. [UNESP] Melo, Guilherme D. [UNESP] Ramos, Adriano T. Maiorka, Paulo C. |
dc.subject.por.fl_str_mv |
Animal model Astrocytes Inflammation Lymphocytes Matrix metalloproteinases Bovine herpespirus 5 antigen cell antigen dexamethasone gelatinase B unclassified drug virus antigen animal cell animal experiment animal model animal tissue antibody titer astrocyte B lymphocyte Bovine herpes virus brain brain cortex CD3+ T lymphocyte cellular immunity central nervous system controlled study enzyme activity experimental model herpes virus infection immunohistochemistry immunopathogenesis inoculation lymphocyte lymphocytic infiltration male nerve cell neurologic disease nonhuman nose priority journal rabbit respiratory tract disease suspension cell culture symptomatology T lymphocyte trigeminus ganglion Animals Brain Cattle Disease Models, Animal Encephalitis, Viral Herpesviridae Infections Herpesvirus 5, Bovine Male Matrix Metalloproteinase 9 Meningoencephalitis Rabbits |
topic |
Animal model Astrocytes Inflammation Lymphocytes Matrix metalloproteinases Bovine herpespirus 5 antigen cell antigen dexamethasone gelatinase B unclassified drug virus antigen animal cell animal experiment animal model animal tissue antibody titer astrocyte B lymphocyte Bovine herpes virus brain brain cortex CD3+ T lymphocyte cellular immunity central nervous system controlled study enzyme activity experimental model herpes virus infection immunohistochemistry immunopathogenesis inoculation lymphocyte lymphocytic infiltration male nerve cell neurologic disease nonhuman nose priority journal rabbit respiratory tract disease suspension cell culture symptomatology T lymphocyte trigeminus ganglion Animals Brain Cattle Disease Models, Animal Encephalitis, Viral Herpesviridae Infections Herpesvirus 5, Bovine Male Matrix Metalloproteinase 9 Meningoencephalitis Rabbits |
description |
Since little information is available regarding cellular antigen mapping and the involvement of non-neuronal cells in the pathogenesis of bovine herpesvirus type 5 (BHV-5) infection, it were determined the BHV-5 distribution, the astrocytic reactivity, the involvement of lymphocytes and the presence of matrix metalloproteinase (MMP)-9 in the brain of rabbits experimentally infected with BHV-5. Twelve New Zealand rabbits that were seronegative for BHV-5 were used for virus inoculation, and five rabbits were used as mock-infected controls. The rabbits were kept in separate areas and were inoculated intranasally with 500 μl of virus suspension (EVI 88 Brazilian isolate) into each nostril (virus titer, 107.5 TCID50). Control rabbits were inoculated with the same volume of minimum essential medium. Five days before virus inoculation, the rabbits were submitted to daily administration of dexamethasone. After virus inoculation, the rabbits were monitored clinically on a daily basis. Seven rabbits showed respiratory symptoms and four animals exhibited neurological symptoms. Tissue sections were collected for histological examination and immunohistochemistry to examine BHV-5 antigens, astrocytes, T and B lymphocytes and MMP-9. By means of immunohistochemical and PCR methods, BHV-5 was detected in the entire brain of the animals which presented with neurological symptoms, especially in the trigeminal ganglion and cerebral cortices. Furthermore, BHV-5 antigens were detected in neurons and/or other non-neural cells. In addition to the neurons, most infiltrating CD3 T lymphocytes observed in these areas were positive for MMP-9 and also for BHV-5 antigen. These infected cells might contribute to the spread of the virus to the rabbit brain along the trigeminal ganglia and olfactory nerve pathways. © 2013 Elsevier Ltd. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-04-01 2014-05-27T11:28:45Z 2014-05-27T11:28:45Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.micpath.2013.01.003 Microbial Pathogenesis, v. 57, p. 10-16. 0882-4010 1096-1208 http://hdl.handle.net/11449/74935 10.1016/j.micpath.2013.01.003 WOS:000316921400002 2-s2.0-84873968605 2-s2.0-84873968605.pdf 0310405558125634 2976129874554698 |
url |
http://dx.doi.org/10.1016/j.micpath.2013.01.003 http://hdl.handle.net/11449/74935 |
identifier_str_mv |
Microbial Pathogenesis, v. 57, p. 10-16. 0882-4010 1096-1208 10.1016/j.micpath.2013.01.003 WOS:000316921400002 2-s2.0-84873968605 2-s2.0-84873968605.pdf 0310405558125634 2976129874554698 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Microbial Pathogenesis 2.332 0,751 0,751 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
10-16 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797790182010781696 |