In vitro antimicrobial activity of auxiliary chemical substances and natural extracts on Candida albicans and Enterococcus faecalis in root canals
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/1678-7757201302135 http://hdl.handle.net/11449/74739 |
Resumo: | Objective: The aim of this study was to evaluate the antimicrobial activity of auxiliary chemical substances and natural extracts on Candida albicans and Enterococcus faecali's inoculated in root canals. Material and Methods: Seventy-two human tooth roots were contaminated with C. albicans and E. faecalis for 21 days. The groups were divided according to the auxiliary chemical substance into: G1) 2.5% sodium hypochlorite (NaOCl), G2) 2% chlorhexidine gel (CHX), G3) castor oil, G4) glycolic Aloe vera extract, g5) glycolic ginger extract, and G6) sterile saline (control). The samples of the root canal were collected at different intervals: confirmation collection, at 21 days after contamination; 1st collection, after instrumentation; and 2nd collection, seven days after instrumentation. Microbiological samples were grown in culture medium and incubated at 37°C for 48 hours. Results: The results were submitted to the Kruskal-Wallis and Dunn (5%) statistical tests. NaOCl and CHX completely eliminated the microorganisms of the root canals. Castor oil and ginger significantly reduced the number of CFU of the tested bacteria. Reduction of CFU/mL at the 1st and 2nd collections for groups G1, G2, G3 and G4 was greater in comparison to groups G5 and G6. Conclusion: It was concluded that 2.5% sodium hypochlorite and 2% chlorhexidine gel were more effective in eliminating C. albicans and E. faecalis, followed by the castor oil and glycolic ginger extract. The Aloe vera extract showed no antimicrobial activity. |
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In vitro antimicrobial activity of auxiliary chemical substances and natural extracts on Candida albicans and Enterococcus faecalis in root canalsAloe veraChlorhexidineRicinus communisSodium hypochloriteZingiber officinaleObjective: The aim of this study was to evaluate the antimicrobial activity of auxiliary chemical substances and natural extracts on Candida albicans and Enterococcus faecali's inoculated in root canals. Material and Methods: Seventy-two human tooth roots were contaminated with C. albicans and E. faecalis for 21 days. The groups were divided according to the auxiliary chemical substance into: G1) 2.5% sodium hypochlorite (NaOCl), G2) 2% chlorhexidine gel (CHX), G3) castor oil, G4) glycolic Aloe vera extract, g5) glycolic ginger extract, and G6) sterile saline (control). The samples of the root canal were collected at different intervals: confirmation collection, at 21 days after contamination; 1st collection, after instrumentation; and 2nd collection, seven days after instrumentation. Microbiological samples were grown in culture medium and incubated at 37°C for 48 hours. Results: The results were submitted to the Kruskal-Wallis and Dunn (5%) statistical tests. NaOCl and CHX completely eliminated the microorganisms of the root canals. Castor oil and ginger significantly reduced the number of CFU of the tested bacteria. Reduction of CFU/mL at the 1st and 2nd collections for groups G1, G2, G3 and G4 was greater in comparison to groups G5 and G6. Conclusion: It was concluded that 2.5% sodium hypochlorite and 2% chlorhexidine gel were more effective in eliminating C. albicans and E. faecalis, followed by the castor oil and glycolic ginger extract. The Aloe vera extract showed no antimicrobial activity.Restorative Dentistry Department Univ. Estadual Paulista - UNESP São José dos Campos, São Paulo, SPBiosciences and Oral Diagnosis Department Univ. Estadual Paulista - UNESP São José dos Campos, São Paulo, SPOral Diagnosis and Biosciences Department, Univ Estadual Paulista - UNESP São José dos Campos, São Paulo, SPSchool of Dentistry of São José dos Campos Univ. Estadual Paulista - UNESP São José dos Campos, São Paulo, SPRestorative Dentistry Department Univ. Estadual Paulista - UNESP São José dos Campos, São Paulo, SPBiosciences and Oral Diagnosis Department Univ. Estadual Paulista - UNESP São José dos Campos, São Paulo, SPOral Diagnosis and Biosciences Department, Univ Estadual Paulista - UNESP São José dos Campos, São Paulo, SPSchool of Dentistry of São José dos Campos Univ. Estadual Paulista - UNESP São José dos Campos, São Paulo, SPUniversidade Estadual Paulista (Unesp)Valera, Marcia Carneiro [UNESP]Maekawa, Lilian Eiko [UNESP]Oliveira, Luciane Dias de [UNESP]Jorge, Antonio Olavo Cardoso [UNESP]Shygei, Érika [UNESP]Carvalho, Cláudio Antonio Talge [UNESP]2014-05-27T11:28:36Z2014-05-27T11:28:36Z2013-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article118-123application/pdfhttp://dx.doi.org/10.1590/1678-7757201302135Journal of Applied Oral Science, v. 21, n. 2, p. 118-123, 2013.1678-77571678-7765http://hdl.handle.net/11449/7473910.1590/1678-7757201302135S1678-7757201302135WOS:0003199977000052-s2.0-848781824642-s2.0-84878182464.pdf4716676611330311005356715362356993046001665831000000-0003-0987-5594Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Applied Oral Science1.7090,645info:eu-repo/semantics/openAccess2024-01-08T06:22:59Zoai:repositorio.unesp.br:11449/74739Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-08T06:22:59Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
In vitro antimicrobial activity of auxiliary chemical substances and natural extracts on Candida albicans and Enterococcus faecalis in root canals |
title |
In vitro antimicrobial activity of auxiliary chemical substances and natural extracts on Candida albicans and Enterococcus faecalis in root canals |
spellingShingle |
In vitro antimicrobial activity of auxiliary chemical substances and natural extracts on Candida albicans and Enterococcus faecalis in root canals Valera, Marcia Carneiro [UNESP] Aloe vera Chlorhexidine Ricinus communis Sodium hypochlorite Zingiber officinale |
title_short |
In vitro antimicrobial activity of auxiliary chemical substances and natural extracts on Candida albicans and Enterococcus faecalis in root canals |
title_full |
In vitro antimicrobial activity of auxiliary chemical substances and natural extracts on Candida albicans and Enterococcus faecalis in root canals |
title_fullStr |
In vitro antimicrobial activity of auxiliary chemical substances and natural extracts on Candida albicans and Enterococcus faecalis in root canals |
title_full_unstemmed |
In vitro antimicrobial activity of auxiliary chemical substances and natural extracts on Candida albicans and Enterococcus faecalis in root canals |
title_sort |
In vitro antimicrobial activity of auxiliary chemical substances and natural extracts on Candida albicans and Enterococcus faecalis in root canals |
author |
Valera, Marcia Carneiro [UNESP] |
author_facet |
Valera, Marcia Carneiro [UNESP] Maekawa, Lilian Eiko [UNESP] Oliveira, Luciane Dias de [UNESP] Jorge, Antonio Olavo Cardoso [UNESP] Shygei, Érika [UNESP] Carvalho, Cláudio Antonio Talge [UNESP] |
author_role |
author |
author2 |
Maekawa, Lilian Eiko [UNESP] Oliveira, Luciane Dias de [UNESP] Jorge, Antonio Olavo Cardoso [UNESP] Shygei, Érika [UNESP] Carvalho, Cláudio Antonio Talge [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Valera, Marcia Carneiro [UNESP] Maekawa, Lilian Eiko [UNESP] Oliveira, Luciane Dias de [UNESP] Jorge, Antonio Olavo Cardoso [UNESP] Shygei, Érika [UNESP] Carvalho, Cláudio Antonio Talge [UNESP] |
dc.subject.por.fl_str_mv |
Aloe vera Chlorhexidine Ricinus communis Sodium hypochlorite Zingiber officinale |
topic |
Aloe vera Chlorhexidine Ricinus communis Sodium hypochlorite Zingiber officinale |
description |
Objective: The aim of this study was to evaluate the antimicrobial activity of auxiliary chemical substances and natural extracts on Candida albicans and Enterococcus faecali's inoculated in root canals. Material and Methods: Seventy-two human tooth roots were contaminated with C. albicans and E. faecalis for 21 days. The groups were divided according to the auxiliary chemical substance into: G1) 2.5% sodium hypochlorite (NaOCl), G2) 2% chlorhexidine gel (CHX), G3) castor oil, G4) glycolic Aloe vera extract, g5) glycolic ginger extract, and G6) sterile saline (control). The samples of the root canal were collected at different intervals: confirmation collection, at 21 days after contamination; 1st collection, after instrumentation; and 2nd collection, seven days after instrumentation. Microbiological samples were grown in culture medium and incubated at 37°C for 48 hours. Results: The results were submitted to the Kruskal-Wallis and Dunn (5%) statistical tests. NaOCl and CHX completely eliminated the microorganisms of the root canals. Castor oil and ginger significantly reduced the number of CFU of the tested bacteria. Reduction of CFU/mL at the 1st and 2nd collections for groups G1, G2, G3 and G4 was greater in comparison to groups G5 and G6. Conclusion: It was concluded that 2.5% sodium hypochlorite and 2% chlorhexidine gel were more effective in eliminating C. albicans and E. faecalis, followed by the castor oil and glycolic ginger extract. The Aloe vera extract showed no antimicrobial activity. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-03-01 2014-05-27T11:28:36Z 2014-05-27T11:28:36Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1678-7757201302135 Journal of Applied Oral Science, v. 21, n. 2, p. 118-123, 2013. 1678-7757 1678-7765 http://hdl.handle.net/11449/74739 10.1590/1678-7757201302135 S1678-7757201302135 WOS:000319997700005 2-s2.0-84878182464 2-s2.0-84878182464.pdf 4716676611330311 0053567153623569 9304600166583100 0000-0003-0987-5594 |
url |
http://dx.doi.org/10.1590/1678-7757201302135 http://hdl.handle.net/11449/74739 |
identifier_str_mv |
Journal of Applied Oral Science, v. 21, n. 2, p. 118-123, 2013. 1678-7757 1678-7765 10.1590/1678-7757201302135 S1678-7757201302135 WOS:000319997700005 2-s2.0-84878182464 2-s2.0-84878182464.pdf 4716676611330311 0053567153623569 9304600166583100 0000-0003-0987-5594 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Applied Oral Science 1.709 0,645 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
118-123 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1792962285462880256 |