Organization and Chromosomal Distribution of Histone Genes and Transposable Rex Elements in the Genome of Astyanax bockmanni (Teleostei, Characiformes)
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1159/000441613 http://hdl.handle.net/11449/172422 |
Resumo: | An important feature of eukaryotic organisms is the number of different repetitive DNA sequences in their genome, a feature not observed in prokaryotes. These sequences are considered to be important components for understanding evolutionary mechanisms and the karyotypic differentiation processes. Thus, we aimed to physically map the histone genes and transposable elements of the Rex family in 6 fish populations of Astyanax bockmanni. FISH results using a histone H1 gene probe showed fluorescent clusters in 2 chromosome pairs in all 6 samples analyzed. In contrast, FISH with a histone H3 probe showed conspicuous blocks in 4 chromosomes in 5 of the 6 populations analyzed. The sixth population revealed 7 chromosomes marked with this probe. Probes for the transposable elements Rex1 and Rex6 showed small sites dispersed on most chromosomes of the 6 populations, and the Rex3 element is located in a big block concentrated in only 1 acrocentric chromosome of 2 populations. As for the other populations, a Rex3 probe showed large blocks in more than 1 chromosome. Fish from Alambari and Campo Novo Stream have Rex3 elements dispersed along most of the chromosomes. Additionally, the conspicuous signals of Rex1, Rex3, and Rex6 were identified in the acrocentric B microchromosome of A. bockmanni found only in individuals of the Alambari River. Thus, we believe that different mechanisms drive the spread of repetitive sequences among the populations analyzed, which appear to be organized differently in the genome of A. bockmanni. The presence of transposable elements in the B chromosome also suggests that these sequences could play a role in the origin and maintenance of the supernumerary element in the genome of this species. |
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Organization and Chromosomal Distribution of Histone Genes and Transposable Rex Elements in the Genome of Astyanax bockmanni (Teleostei, Characiformes)Histone genesNeotropical fishRepetitive DNATransposable Rex elementsAn important feature of eukaryotic organisms is the number of different repetitive DNA sequences in their genome, a feature not observed in prokaryotes. These sequences are considered to be important components for understanding evolutionary mechanisms and the karyotypic differentiation processes. Thus, we aimed to physically map the histone genes and transposable elements of the Rex family in 6 fish populations of Astyanax bockmanni. FISH results using a histone H1 gene probe showed fluorescent clusters in 2 chromosome pairs in all 6 samples analyzed. In contrast, FISH with a histone H3 probe showed conspicuous blocks in 4 chromosomes in 5 of the 6 populations analyzed. The sixth population revealed 7 chromosomes marked with this probe. Probes for the transposable elements Rex1 and Rex6 showed small sites dispersed on most chromosomes of the 6 populations, and the Rex3 element is located in a big block concentrated in only 1 acrocentric chromosome of 2 populations. As for the other populations, a Rex3 probe showed large blocks in more than 1 chromosome. Fish from Alambari and Campo Novo Stream have Rex3 elements dispersed along most of the chromosomes. Additionally, the conspicuous signals of Rex1, Rex3, and Rex6 were identified in the acrocentric B microchromosome of A. bockmanni found only in individuals of the Alambari River. Thus, we believe that different mechanisms drive the spread of repetitive sequences among the populations analyzed, which appear to be organized differently in the genome of A. bockmanni. The presence of transposable elements in the B chromosome also suggests that these sequences could play a role in the origin and maintenance of the supernumerary element in the genome of this species.Departamento de Ciências Biológicas Faculdade de Ciências Universidade Estadual Paulista (UNESP)Departamento de Morfologia Instituto de Biociências UNESPCentro de Aquicultura de Jaboticabal (CAUNESP) UNESPInstituto de Biociências Universidade Federal de Mato Grosso (UFTM)Departamento de Ciências Biológicas Faculdade de Ciências Universidade Estadual Paulista (UNESP)Departamento de Morfologia Instituto de Biociências UNESPCentro de Aquicultura de Jaboticabal (CAUNESP) UNESPUniversidade Estadual Paulista (Unesp)Universidade Federal de Mato Grosso (UFTM)Daniel, Sandro N. [UNESP]Penitente, Manolo [UNESP]Silva, Duílio M.Z.A. [UNESP]Hashimoto, Diogo T. [UNESP]Ferreira, Daniela C.Foresti, Fausto [UNESP]Porto-Foresti, Fábio [UNESP]2018-12-11T17:00:13Z2018-12-11T17:00:13Z2015-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article311-318application/pdfhttp://dx.doi.org/10.1159/000441613Cytogenetic and Genome Research, v. 146, n. 4, p. 311-318, 2015.1424-859X1424-8581http://hdl.handle.net/11449/17242210.1159/0004416132-s2.0-849543638452-s2.0-84954363845.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCytogenetic and Genome Research0,748info:eu-repo/semantics/openAccess2024-04-09T15:10:46Zoai:repositorio.unesp.br:11449/172422Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-04-09T15:10:46Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Organization and Chromosomal Distribution of Histone Genes and Transposable Rex Elements in the Genome of Astyanax bockmanni (Teleostei, Characiformes) |
title |
Organization and Chromosomal Distribution of Histone Genes and Transposable Rex Elements in the Genome of Astyanax bockmanni (Teleostei, Characiformes) |
spellingShingle |
Organization and Chromosomal Distribution of Histone Genes and Transposable Rex Elements in the Genome of Astyanax bockmanni (Teleostei, Characiformes) Daniel, Sandro N. [UNESP] Histone genes Neotropical fish Repetitive DNA Transposable Rex elements |
title_short |
Organization and Chromosomal Distribution of Histone Genes and Transposable Rex Elements in the Genome of Astyanax bockmanni (Teleostei, Characiformes) |
title_full |
Organization and Chromosomal Distribution of Histone Genes and Transposable Rex Elements in the Genome of Astyanax bockmanni (Teleostei, Characiformes) |
title_fullStr |
Organization and Chromosomal Distribution of Histone Genes and Transposable Rex Elements in the Genome of Astyanax bockmanni (Teleostei, Characiformes) |
title_full_unstemmed |
Organization and Chromosomal Distribution of Histone Genes and Transposable Rex Elements in the Genome of Astyanax bockmanni (Teleostei, Characiformes) |
title_sort |
Organization and Chromosomal Distribution of Histone Genes and Transposable Rex Elements in the Genome of Astyanax bockmanni (Teleostei, Characiformes) |
author |
Daniel, Sandro N. [UNESP] |
author_facet |
Daniel, Sandro N. [UNESP] Penitente, Manolo [UNESP] Silva, Duílio M.Z.A. [UNESP] Hashimoto, Diogo T. [UNESP] Ferreira, Daniela C. Foresti, Fausto [UNESP] Porto-Foresti, Fábio [UNESP] |
author_role |
author |
author2 |
Penitente, Manolo [UNESP] Silva, Duílio M.Z.A. [UNESP] Hashimoto, Diogo T. [UNESP] Ferreira, Daniela C. Foresti, Fausto [UNESP] Porto-Foresti, Fábio [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal de Mato Grosso (UFTM) |
dc.contributor.author.fl_str_mv |
Daniel, Sandro N. [UNESP] Penitente, Manolo [UNESP] Silva, Duílio M.Z.A. [UNESP] Hashimoto, Diogo T. [UNESP] Ferreira, Daniela C. Foresti, Fausto [UNESP] Porto-Foresti, Fábio [UNESP] |
dc.subject.por.fl_str_mv |
Histone genes Neotropical fish Repetitive DNA Transposable Rex elements |
topic |
Histone genes Neotropical fish Repetitive DNA Transposable Rex elements |
description |
An important feature of eukaryotic organisms is the number of different repetitive DNA sequences in their genome, a feature not observed in prokaryotes. These sequences are considered to be important components for understanding evolutionary mechanisms and the karyotypic differentiation processes. Thus, we aimed to physically map the histone genes and transposable elements of the Rex family in 6 fish populations of Astyanax bockmanni. FISH results using a histone H1 gene probe showed fluorescent clusters in 2 chromosome pairs in all 6 samples analyzed. In contrast, FISH with a histone H3 probe showed conspicuous blocks in 4 chromosomes in 5 of the 6 populations analyzed. The sixth population revealed 7 chromosomes marked with this probe. Probes for the transposable elements Rex1 and Rex6 showed small sites dispersed on most chromosomes of the 6 populations, and the Rex3 element is located in a big block concentrated in only 1 acrocentric chromosome of 2 populations. As for the other populations, a Rex3 probe showed large blocks in more than 1 chromosome. Fish from Alambari and Campo Novo Stream have Rex3 elements dispersed along most of the chromosomes. Additionally, the conspicuous signals of Rex1, Rex3, and Rex6 were identified in the acrocentric B microchromosome of A. bockmanni found only in individuals of the Alambari River. Thus, we believe that different mechanisms drive the spread of repetitive sequences among the populations analyzed, which appear to be organized differently in the genome of A. bockmanni. The presence of transposable elements in the B chromosome also suggests that these sequences could play a role in the origin and maintenance of the supernumerary element in the genome of this species. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-12-01 2018-12-11T17:00:13Z 2018-12-11T17:00:13Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1159/000441613 Cytogenetic and Genome Research, v. 146, n. 4, p. 311-318, 2015. 1424-859X 1424-8581 http://hdl.handle.net/11449/172422 10.1159/000441613 2-s2.0-84954363845 2-s2.0-84954363845.pdf |
url |
http://dx.doi.org/10.1159/000441613 http://hdl.handle.net/11449/172422 |
identifier_str_mv |
Cytogenetic and Genome Research, v. 146, n. 4, p. 311-318, 2015. 1424-859X 1424-8581 10.1159/000441613 2-s2.0-84954363845 2-s2.0-84954363845.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Cytogenetic and Genome Research 0,748 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
311-318 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797789446881411072 |