Evaluation of macrophage activity and antibody production in genetically selected mice infected with Leptospira serovar pomona
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1678-91992008000100018 http://hdl.handle.net/11449/39835 |
Resumo: | The aim of the present study was to evaluate the role of macrophage activity and antibody production in experimental infection with Leptospira Pomona in mice genetically selected for high (H) or low (L) humoral immune response. To evaluate macrophage activity, reactive oxygen and nitrogen intermediates were determined. Also, the production of tumor necrosis factor (TNF-alpha) and the recovery of Leptospira-specific antibodies in the kidneys and liver were assessed; histological lesions were analyzed using the hematoxylin-eosin technique, and Leptospira antigens in tissues were determined by immunohistochemistry. Results showed that recovery of microorganisms from the analyzed organs was lower in LIV-A mice. However, HIV-A animals showed total restraint since the 14th day after infection, whereas LIV-A mice still had bacteria in the liver at the 21st post-infection day. Immune response against Pomona serovar in those lineages was characterized as high production of antibodies, mainly in late periods of the infectious process. The production of reactive oxygen and nitrogen intermediates also contributed to the elimination of Leptospira Pomona in all two lineages; H2O2 production was an important factor in HIV-A mice, as well as NO production in the LIV-A animals, mainly at the latest post-inoculation periods. The same occurred regarding TNF-alpha production. Severe renal lesions were observed at periods in which larger numbers of leptospires were isolated using the culture technique. Tissue alterations persisted in LIV-A mice, even at periods in which leptospires were not recovered. Immunohistochemistry showed to be more sensitive than culturing. However, both techniques were appropriate for the agent identification in the studied lineages. Results suggest that such lineages could represent an important model to investigate pathogenesis and immune response against the varied serovars of leptospires. |
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Evaluation of macrophage activity and antibody production in genetically selected mice infected with Leptospira serovar pomonaimmunological testsBiozzi miceexperimental infectionLeptospira interrogansserovar PomonamacrophagesThe aim of the present study was to evaluate the role of macrophage activity and antibody production in experimental infection with Leptospira Pomona in mice genetically selected for high (H) or low (L) humoral immune response. To evaluate macrophage activity, reactive oxygen and nitrogen intermediates were determined. Also, the production of tumor necrosis factor (TNF-alpha) and the recovery of Leptospira-specific antibodies in the kidneys and liver were assessed; histological lesions were analyzed using the hematoxylin-eosin technique, and Leptospira antigens in tissues were determined by immunohistochemistry. Results showed that recovery of microorganisms from the analyzed organs was lower in LIV-A mice. However, HIV-A animals showed total restraint since the 14th day after infection, whereas LIV-A mice still had bacteria in the liver at the 21st post-infection day. Immune response against Pomona serovar in those lineages was characterized as high production of antibodies, mainly in late periods of the infectious process. The production of reactive oxygen and nitrogen intermediates also contributed to the elimination of Leptospira Pomona in all two lineages; H2O2 production was an important factor in HIV-A mice, as well as NO production in the LIV-A animals, mainly at the latest post-inoculation periods. The same occurred regarding TNF-alpha production. Severe renal lesions were observed at periods in which larger numbers of leptospires were isolated using the culture technique. Tissue alterations persisted in LIV-A mice, even at periods in which leptospires were not recovered. Immunohistochemistry showed to be more sensitive than culturing. However, both techniques were appropriate for the agent identification in the studied lineages. Results suggest that such lineages could represent an important model to investigate pathogenesis and immune response against the varied serovars of leptospires.UNESP, São Paulo State Univ, Botucatu, SP, BrazilUNESP, São Paulo State Univ, Botucatu, SP, BrazilUniversidade Estadual Paulista (Unesp), Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP)Universidade Estadual Paulista (Unesp)Haanwinckel, M. C. S. [UNESP]2014-05-20T15:30:28Z2014-05-20T15:30:28Z2008-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article189-189application/pdfhttp://dx.doi.org/10.1590/S1678-91992008000100018Journal of Venomous Animals and Toxins Including Tropical Diseases. Botucatu: Cevap-unesp, v. 14, n. 1, p. 189-189, 2008.1678-9199http://hdl.handle.net/11449/39835S1678-91992008000100018WOS:000254511600018S1678-91992008000100018.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Venomous Animals and Toxins Including Tropical Diseases1.7820,573info:eu-repo/semantics/openAccess2023-10-14T06:10:41Zoai:repositorio.unesp.br:11449/39835Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-14T06:10:41Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Evaluation of macrophage activity and antibody production in genetically selected mice infected with Leptospira serovar pomona |
title |
Evaluation of macrophage activity and antibody production in genetically selected mice infected with Leptospira serovar pomona |
spellingShingle |
Evaluation of macrophage activity and antibody production in genetically selected mice infected with Leptospira serovar pomona Haanwinckel, M. C. S. [UNESP] immunological tests Biozzi mice experimental infection Leptospira interrogans serovar Pomona macrophages |
title_short |
Evaluation of macrophage activity and antibody production in genetically selected mice infected with Leptospira serovar pomona |
title_full |
Evaluation of macrophage activity and antibody production in genetically selected mice infected with Leptospira serovar pomona |
title_fullStr |
Evaluation of macrophage activity and antibody production in genetically selected mice infected with Leptospira serovar pomona |
title_full_unstemmed |
Evaluation of macrophage activity and antibody production in genetically selected mice infected with Leptospira serovar pomona |
title_sort |
Evaluation of macrophage activity and antibody production in genetically selected mice infected with Leptospira serovar pomona |
author |
Haanwinckel, M. C. S. [UNESP] |
author_facet |
Haanwinckel, M. C. S. [UNESP] |
author_role |
author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Haanwinckel, M. C. S. [UNESP] |
dc.subject.por.fl_str_mv |
immunological tests Biozzi mice experimental infection Leptospira interrogans serovar Pomona macrophages |
topic |
immunological tests Biozzi mice experimental infection Leptospira interrogans serovar Pomona macrophages |
description |
The aim of the present study was to evaluate the role of macrophage activity and antibody production in experimental infection with Leptospira Pomona in mice genetically selected for high (H) or low (L) humoral immune response. To evaluate macrophage activity, reactive oxygen and nitrogen intermediates were determined. Also, the production of tumor necrosis factor (TNF-alpha) and the recovery of Leptospira-specific antibodies in the kidneys and liver were assessed; histological lesions were analyzed using the hematoxylin-eosin technique, and Leptospira antigens in tissues were determined by immunohistochemistry. Results showed that recovery of microorganisms from the analyzed organs was lower in LIV-A mice. However, HIV-A animals showed total restraint since the 14th day after infection, whereas LIV-A mice still had bacteria in the liver at the 21st post-infection day. Immune response against Pomona serovar in those lineages was characterized as high production of antibodies, mainly in late periods of the infectious process. The production of reactive oxygen and nitrogen intermediates also contributed to the elimination of Leptospira Pomona in all two lineages; H2O2 production was an important factor in HIV-A mice, as well as NO production in the LIV-A animals, mainly at the latest post-inoculation periods. The same occurred regarding TNF-alpha production. Severe renal lesions were observed at periods in which larger numbers of leptospires were isolated using the culture technique. Tissue alterations persisted in LIV-A mice, even at periods in which leptospires were not recovered. Immunohistochemistry showed to be more sensitive than culturing. However, both techniques were appropriate for the agent identification in the studied lineages. Results suggest that such lineages could represent an important model to investigate pathogenesis and immune response against the varied serovars of leptospires. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-01-01 2014-05-20T15:30:28Z 2014-05-20T15:30:28Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1678-91992008000100018 Journal of Venomous Animals and Toxins Including Tropical Diseases. Botucatu: Cevap-unesp, v. 14, n. 1, p. 189-189, 2008. 1678-9199 http://hdl.handle.net/11449/39835 S1678-91992008000100018 WOS:000254511600018 S1678-91992008000100018.pdf |
url |
http://dx.doi.org/10.1590/S1678-91992008000100018 http://hdl.handle.net/11449/39835 |
identifier_str_mv |
Journal of Venomous Animals and Toxins Including Tropical Diseases. Botucatu: Cevap-unesp, v. 14, n. 1, p. 189-189, 2008. 1678-9199 S1678-91992008000100018 WOS:000254511600018 S1678-91992008000100018.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Venomous Animals and Toxins Including Tropical Diseases 1.782 0,573 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
189-189 application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual Paulista (Unesp), Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP) |
publisher.none.fl_str_mv |
Universidade Estadual Paulista (Unesp), Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP) |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964564231553024 |