Systemic and local effects of doxycycline and low-intensity laser treatment on periodontitis in rats
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.5051/JPIS.2100420021 http://hdl.handle.net/11449/233740 |
Resumo: | Purpose: This study evaluated the systemic and local effects of doxycycline (DOX) and lowintensity laser (LIL) treatment as adjuvants to scaling and root planing (SRP) in the treatment of experimental periodontitis in rats. Methods: The sample consisted of 180 male rats (Rattus norvegicus albinus, Wistar), of which 30 did not receive induction of periodontal disease (negative control [NC] group) and 150 received induction of periodontal disease in the lower first molar. After 7 days, the ligature was removed, and the animals were divided into the following groups: NT (no treatment), SRP (SRP), DOX (SRP and DOX irrigation), LIL (SRP and laser irradiation), and DOX+LIL (SRP, DOX, and LIL). The animals were euthanized at 7, 15, and 30 days; thereafter, biochemical, radiographic, histological, and immunohistochemical analyses were performed. Results: In the intragroup analysis, lower concentrations of α-1-glycoprotein acid (α-1-Ga) and complement 3 (C3) were observed in the DOX+LIL group than in all other groups at all time points, as well as lower levels of complement 4 (C4) at 15 and 30 days (P<0.001). Less bone loss was observed in the DOX, LIL, and DOX+LIL groups than in the NC and SRP groups at all time points (P<0.001). There was a smaller number of tartrate-resistant acid phosphatase (TRAP)-positive cells in the DOX+LIL group than in the other groups at all time points (P<0.001). Positive correlations were observed between the systemic levels of α-1-Ga, C3, and C4 and the number of TRAP-positive cells. Conclusions: The combination of DOX with LIL as SRP adjuvants was effective both systemically and locally for the treatment of experimental periodontitis in rats. |
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Systemic and local effects of doxycycline and low-intensity laser treatment on periodontitis in ratsDoxycyclineLasersPeriodontal diseasePeriodontitisRatsPurpose: This study evaluated the systemic and local effects of doxycycline (DOX) and lowintensity laser (LIL) treatment as adjuvants to scaling and root planing (SRP) in the treatment of experimental periodontitis in rats. Methods: The sample consisted of 180 male rats (Rattus norvegicus albinus, Wistar), of which 30 did not receive induction of periodontal disease (negative control [NC] group) and 150 received induction of periodontal disease in the lower first molar. After 7 days, the ligature was removed, and the animals were divided into the following groups: NT (no treatment), SRP (SRP), DOX (SRP and DOX irrigation), LIL (SRP and laser irradiation), and DOX+LIL (SRP, DOX, and LIL). The animals were euthanized at 7, 15, and 30 days; thereafter, biochemical, radiographic, histological, and immunohistochemical analyses were performed. Results: In the intragroup analysis, lower concentrations of α-1-glycoprotein acid (α-1-Ga) and complement 3 (C3) were observed in the DOX+LIL group than in all other groups at all time points, as well as lower levels of complement 4 (C4) at 15 and 30 days (P<0.001). Less bone loss was observed in the DOX, LIL, and DOX+LIL groups than in the NC and SRP groups at all time points (P<0.001). There was a smaller number of tartrate-resistant acid phosphatase (TRAP)-positive cells in the DOX+LIL group than in the other groups at all time points (P<0.001). Positive correlations were observed between the systemic levels of α-1-Ga, C3, and C4 and the number of TRAP-positive cells. Conclusions: The combination of DOX with LIL as SRP adjuvants was effective both systemically and locally for the treatment of experimental periodontitis in rats.Federal University of Alfenas (UNIFAL-MG) Faculty of DentistryDepartment of Restorative Dentistry São Paulo State University (UNESP) Faculty of DentistryDepartment of Biochemistry Federal University of Alfenas (UNIFAL-MG) Faculty of DentistryDepartment of Basic Sciences São Paulo State University (UNESP) Faculty of DentistryDepartment of Restorative Dentistry São Paulo State University (UNESP) Faculty of DentistryDepartment of Basic Sciences São Paulo State University (UNESP) Faculty of DentistryFederal University of Alfenas (UNIFAL-MG) Faculty of DentistryUniversidade Estadual Paulista (UNESP)Silveira, Glauco Rodrigues Carmode Lima, Daniela CoelhoCintra, Luciano Tavares Ângelo [UNESP]Brigagão, Maísa Ribeiro Pereira LimaErvolino, Edilson [UNESP]Fernandes, Leandro Araújo2022-05-01T09:47:29Z2022-05-01T09:47:29Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-15http://dx.doi.org/10.5051/JPIS.2100420021Journal of Periodontal and Implant Science, v. 51, p. 1-15.2093-22862093-2278http://hdl.handle.net/11449/23374010.5051/JPIS.21004200212-s2.0-85117910062Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Periodontal and Implant Scienceinfo:eu-repo/semantics/openAccess2022-05-01T09:47:29Zoai:repositorio.unesp.br:11449/233740Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-05-01T09:47:29Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Systemic and local effects of doxycycline and low-intensity laser treatment on periodontitis in rats |
title |
Systemic and local effects of doxycycline and low-intensity laser treatment on periodontitis in rats |
spellingShingle |
Systemic and local effects of doxycycline and low-intensity laser treatment on periodontitis in rats Silveira, Glauco Rodrigues Carmo Doxycycline Lasers Periodontal disease Periodontitis Rats |
title_short |
Systemic and local effects of doxycycline and low-intensity laser treatment on periodontitis in rats |
title_full |
Systemic and local effects of doxycycline and low-intensity laser treatment on periodontitis in rats |
title_fullStr |
Systemic and local effects of doxycycline and low-intensity laser treatment on periodontitis in rats |
title_full_unstemmed |
Systemic and local effects of doxycycline and low-intensity laser treatment on periodontitis in rats |
title_sort |
Systemic and local effects of doxycycline and low-intensity laser treatment on periodontitis in rats |
author |
Silveira, Glauco Rodrigues Carmo |
author_facet |
Silveira, Glauco Rodrigues Carmo de Lima, Daniela Coelho Cintra, Luciano Tavares Ângelo [UNESP] Brigagão, Maísa Ribeiro Pereira Lima Ervolino, Edilson [UNESP] Fernandes, Leandro Araújo |
author_role |
author |
author2 |
de Lima, Daniela Coelho Cintra, Luciano Tavares Ângelo [UNESP] Brigagão, Maísa Ribeiro Pereira Lima Ervolino, Edilson [UNESP] Fernandes, Leandro Araújo |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Federal University of Alfenas (UNIFAL-MG) Faculty of Dentistry Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Silveira, Glauco Rodrigues Carmo de Lima, Daniela Coelho Cintra, Luciano Tavares Ângelo [UNESP] Brigagão, Maísa Ribeiro Pereira Lima Ervolino, Edilson [UNESP] Fernandes, Leandro Araújo |
dc.subject.por.fl_str_mv |
Doxycycline Lasers Periodontal disease Periodontitis Rats |
topic |
Doxycycline Lasers Periodontal disease Periodontitis Rats |
description |
Purpose: This study evaluated the systemic and local effects of doxycycline (DOX) and lowintensity laser (LIL) treatment as adjuvants to scaling and root planing (SRP) in the treatment of experimental periodontitis in rats. Methods: The sample consisted of 180 male rats (Rattus norvegicus albinus, Wistar), of which 30 did not receive induction of periodontal disease (negative control [NC] group) and 150 received induction of periodontal disease in the lower first molar. After 7 days, the ligature was removed, and the animals were divided into the following groups: NT (no treatment), SRP (SRP), DOX (SRP and DOX irrigation), LIL (SRP and laser irradiation), and DOX+LIL (SRP, DOX, and LIL). The animals were euthanized at 7, 15, and 30 days; thereafter, biochemical, radiographic, histological, and immunohistochemical analyses were performed. Results: In the intragroup analysis, lower concentrations of α-1-glycoprotein acid (α-1-Ga) and complement 3 (C3) were observed in the DOX+LIL group than in all other groups at all time points, as well as lower levels of complement 4 (C4) at 15 and 30 days (P<0.001). Less bone loss was observed in the DOX, LIL, and DOX+LIL groups than in the NC and SRP groups at all time points (P<0.001). There was a smaller number of tartrate-resistant acid phosphatase (TRAP)-positive cells in the DOX+LIL group than in the other groups at all time points (P<0.001). Positive correlations were observed between the systemic levels of α-1-Ga, C3, and C4 and the number of TRAP-positive cells. Conclusions: The combination of DOX with LIL as SRP adjuvants was effective both systemically and locally for the treatment of experimental periodontitis in rats. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 2022-05-01T09:47:29Z 2022-05-01T09:47:29Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.5051/JPIS.2100420021 Journal of Periodontal and Implant Science, v. 51, p. 1-15. 2093-2286 2093-2278 http://hdl.handle.net/11449/233740 10.5051/JPIS.2100420021 2-s2.0-85117910062 |
url |
http://dx.doi.org/10.5051/JPIS.2100420021 http://hdl.handle.net/11449/233740 |
identifier_str_mv |
Journal of Periodontal and Implant Science, v. 51, p. 1-15. 2093-2286 2093-2278 10.5051/JPIS.2100420021 2-s2.0-85117910062 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Periodontal and Implant Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1-15 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1797789760782073856 |