Chemical and morphological effects of the contraceptive hormone 17 α-ethynylestradiol on fluid lipid membranes
Main Author: | |
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Publication Date: | 2021 |
Other Authors: | , , , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Institucional da UNESP |
Download full: | http://dx.doi.org/10.1016/j.colsurfb.2021.111794 http://hdl.handle.net/11449/208618 |
Summary: | The lack of studies involving the effects in human health associated with the chronic ingestion of pollutants lead to the path of investigating the action of these compounds in cell membrane models. We demonstrated the interaction (causes and consequences) of the hormone 17 α-ethinylestradiol (EE2) with lipid monolayers (prepared as Langmuir films) and bilayers prepared as small unilamellar vesicles (SUVs) and giant unilamellar vesicles (GUVs). Both fluidity and majority chemical composition of real plasma cell membrane were guaranteed using the phospholipid 1-palmitoil-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC). Surface pressure-mean molecular area (π-A) isotherms and PM-IRRAS measurements highlighted the strong interaction of EE2 with POPC monolayers, leading the hormone to remain at the air/water interface and promoting its penetration into the phospholipid hydrophobic chains. In the case of bilayers, the entrance of the hormone inside the SUV is likely facilitated by their high curvature. In GUVs, EE2 was responsible for changes in the spherical shape, forming structures like buds and lipid protrusions. The set of results indicates the strong effects of EE2 on fluid membranes, which is an important feature to predict its damage in human cells. |
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Chemical and morphological effects of the contraceptive hormone 17 α-ethynylestradiol on fluid lipid membranes17α-ethynylestradiolGiant unilamellar vesiclesLangmuir monolayersPM-IRRASPOPCSmall unilamellar vesiclesThe lack of studies involving the effects in human health associated with the chronic ingestion of pollutants lead to the path of investigating the action of these compounds in cell membrane models. We demonstrated the interaction (causes and consequences) of the hormone 17 α-ethinylestradiol (EE2) with lipid monolayers (prepared as Langmuir films) and bilayers prepared as small unilamellar vesicles (SUVs) and giant unilamellar vesicles (GUVs). Both fluidity and majority chemical composition of real plasma cell membrane were guaranteed using the phospholipid 1-palmitoil-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC). Surface pressure-mean molecular area (π-A) isotherms and PM-IRRAS measurements highlighted the strong interaction of EE2 with POPC monolayers, leading the hormone to remain at the air/water interface and promoting its penetration into the phospholipid hydrophobic chains. In the case of bilayers, the entrance of the hormone inside the SUV is likely facilitated by their high curvature. In GUVs, EE2 was responsible for changes in the spherical shape, forming structures like buds and lipid protrusions. The set of results indicates the strong effects of EE2 on fluid membranes, which is an important feature to predict its damage in human cells.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Instituto Nacional de Ciência e Tecnologia em Eletrônica OrgânicaFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Department of Physics School of Technology and Applied Sciences São Paulo State University (UNESP)Institute of Sciences of Food Production Italian National Research Council (CNR-ISPA), S.P. Lecce-MonteroniDepartment of Biological and Environmental Sciences and Technologies (DiSTeBA) University of Salento S.P. Lecce-MonteroniConsorzio Interuniversitario Nazionale per la Scienza e Tecnologia dei Materiali (INSTM) Unità di Lecce, S.P. Lecce-MonteroniDepartment of Physics School of Technology and Applied Sciences São Paulo State University (UNESP)FAPESP: 2016/09633-4FAPESP: 2018/222147-6CAPES: 88882.330129/2018-01Universidade Estadual Paulista (Unesp)Italian National Research Council (CNR-ISPA)S.P. Lecce-MonteroniUnità di LecceRuiz, Gilia Cristine Marques [UNESP]do Carmo Morato, Luis Fernando [UNESP]Pazin, Wallance Moreira [UNESP]Milano, FrancescoConstantino, Carlos José Leopoldo [UNESP]Valli, LudovicoGiotta, Livia2021-06-25T11:15:08Z2021-06-25T11:15:08Z2021-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.colsurfb.2021.111794Colloids and Surfaces B: Biointerfaces, v. 204.1873-43670927-7765http://hdl.handle.net/11449/20861810.1016/j.colsurfb.2021.1117942-s2.0-85104909298Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengColloids and Surfaces B: Biointerfacesinfo:eu-repo/semantics/openAccess2021-10-23T19:02:17Zoai:repositorio.unesp.br:11449/208618Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T19:02:17Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Chemical and morphological effects of the contraceptive hormone 17 α-ethynylestradiol on fluid lipid membranes |
title |
Chemical and morphological effects of the contraceptive hormone 17 α-ethynylestradiol on fluid lipid membranes |
spellingShingle |
Chemical and morphological effects of the contraceptive hormone 17 α-ethynylestradiol on fluid lipid membranes Ruiz, Gilia Cristine Marques [UNESP] 17α-ethynylestradiol Giant unilamellar vesicles Langmuir monolayers PM-IRRAS POPC Small unilamellar vesicles |
title_short |
Chemical and morphological effects of the contraceptive hormone 17 α-ethynylestradiol on fluid lipid membranes |
title_full |
Chemical and morphological effects of the contraceptive hormone 17 α-ethynylestradiol on fluid lipid membranes |
title_fullStr |
Chemical and morphological effects of the contraceptive hormone 17 α-ethynylestradiol on fluid lipid membranes |
title_full_unstemmed |
Chemical and morphological effects of the contraceptive hormone 17 α-ethynylestradiol on fluid lipid membranes |
title_sort |
Chemical and morphological effects of the contraceptive hormone 17 α-ethynylestradiol on fluid lipid membranes |
author |
Ruiz, Gilia Cristine Marques [UNESP] |
author_facet |
Ruiz, Gilia Cristine Marques [UNESP] do Carmo Morato, Luis Fernando [UNESP] Pazin, Wallance Moreira [UNESP] Milano, Francesco Constantino, Carlos José Leopoldo [UNESP] Valli, Ludovico Giotta, Livia |
author_role |
author |
author2 |
do Carmo Morato, Luis Fernando [UNESP] Pazin, Wallance Moreira [UNESP] Milano, Francesco Constantino, Carlos José Leopoldo [UNESP] Valli, Ludovico Giotta, Livia |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Italian National Research Council (CNR-ISPA) S.P. Lecce-Monteroni Unità di Lecce |
dc.contributor.author.fl_str_mv |
Ruiz, Gilia Cristine Marques [UNESP] do Carmo Morato, Luis Fernando [UNESP] Pazin, Wallance Moreira [UNESP] Milano, Francesco Constantino, Carlos José Leopoldo [UNESP] Valli, Ludovico Giotta, Livia |
dc.subject.por.fl_str_mv |
17α-ethynylestradiol Giant unilamellar vesicles Langmuir monolayers PM-IRRAS POPC Small unilamellar vesicles |
topic |
17α-ethynylestradiol Giant unilamellar vesicles Langmuir monolayers PM-IRRAS POPC Small unilamellar vesicles |
description |
The lack of studies involving the effects in human health associated with the chronic ingestion of pollutants lead to the path of investigating the action of these compounds in cell membrane models. We demonstrated the interaction (causes and consequences) of the hormone 17 α-ethinylestradiol (EE2) with lipid monolayers (prepared as Langmuir films) and bilayers prepared as small unilamellar vesicles (SUVs) and giant unilamellar vesicles (GUVs). Both fluidity and majority chemical composition of real plasma cell membrane were guaranteed using the phospholipid 1-palmitoil-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC). Surface pressure-mean molecular area (π-A) isotherms and PM-IRRAS measurements highlighted the strong interaction of EE2 with POPC monolayers, leading the hormone to remain at the air/water interface and promoting its penetration into the phospholipid hydrophobic chains. In the case of bilayers, the entrance of the hormone inside the SUV is likely facilitated by their high curvature. In GUVs, EE2 was responsible for changes in the spherical shape, forming structures like buds and lipid protrusions. The set of results indicates the strong effects of EE2 on fluid membranes, which is an important feature to predict its damage in human cells. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T11:15:08Z 2021-06-25T11:15:08Z 2021-08-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.colsurfb.2021.111794 Colloids and Surfaces B: Biointerfaces, v. 204. 1873-4367 0927-7765 http://hdl.handle.net/11449/208618 10.1016/j.colsurfb.2021.111794 2-s2.0-85104909298 |
url |
http://dx.doi.org/10.1016/j.colsurfb.2021.111794 http://hdl.handle.net/11449/208618 |
identifier_str_mv |
Colloids and Surfaces B: Biointerfaces, v. 204. 1873-4367 0927-7765 10.1016/j.colsurfb.2021.111794 2-s2.0-85104909298 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Colloids and Surfaces B: Biointerfaces |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1797789948246491136 |