Expression of matrix metalloproteinases and PCNA in deep corneal ulcers induced in rabbits, treated with platelet-rich plasma
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/1678-4162-8142 http://hdl.handle.net/11449/165012 |
Resumo: | The aim of this study was to evaluate the influence of platelet-rich (PRP) and poor (PPP) plasma in cell proliferation and matrix metalloproteinases (MMPs) expression during the repair of deep corneal ulcers. Forty-five female rabbits were distributed in 3 experimental groups (G) (n = 15), referred to as PRP (GR), PPP (GP) and Control (GC) groups, in accordance with the treatment. All animals underwent surgical induction of unilateral corneal ulcer. PRP and PPP eye drops were made by using centrifuged blood through standardized protocol, and instilled five times a day. In GC, lubricant eye drops were used. Each group was subdivided (n = 5) according to the final time point, 4 (M4), 7 (M7) and 30 days (M30). The animals' corneas were processed for morphological and immunohistochemical analysis for PCNA, MMP1, MMP2, MMP9, MT1-MMP and TIMP1. In M4, the levels of MMP2 were higher in GP and GR, and in M7, this behavior was only observed in the GP. In M30, more epithelial cells and MMP1 expression were found in GR than GP. In GR, cell proliferation was higher in M4 than at other time points and MMP2 expression was higher in M4 than M30. The PRP stimulates cell proliferation in the early phase (M4) of treatment when compared to other time points, different from other treatments. The use of eye drops of platelet-rich and poor plasma influences the expression of matrix metalloproteinases involved in the corneal repair process. |
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Repositório Institucional da UNESP |
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Expression of matrix metalloproteinases and PCNA in deep corneal ulcers induced in rabbits, treated with platelet-rich plasmaplatelet-poor plasmaMMPPRPhealingcornealThe aim of this study was to evaluate the influence of platelet-rich (PRP) and poor (PPP) plasma in cell proliferation and matrix metalloproteinases (MMPs) expression during the repair of deep corneal ulcers. Forty-five female rabbits were distributed in 3 experimental groups (G) (n = 15), referred to as PRP (GR), PPP (GP) and Control (GC) groups, in accordance with the treatment. All animals underwent surgical induction of unilateral corneal ulcer. PRP and PPP eye drops were made by using centrifuged blood through standardized protocol, and instilled five times a day. In GC, lubricant eye drops were used. Each group was subdivided (n = 5) according to the final time point, 4 (M4), 7 (M7) and 30 days (M30). The animals' corneas were processed for morphological and immunohistochemical analysis for PCNA, MMP1, MMP2, MMP9, MT1-MMP and TIMP1. In M4, the levels of MMP2 were higher in GP and GR, and in M7, this behavior was only observed in the GP. In M30, more epithelial cells and MMP1 expression were found in GR than GP. In GR, cell proliferation was higher in M4 than at other time points and MMP2 expression was higher in M4 than M30. The PRP stimulates cell proliferation in the early phase (M4) of treatment when compared to other time points, different from other treatments. The use of eye drops of platelet-rich and poor plasma influences the expression of matrix metalloproteinases involved in the corneal repair process.Univ Estadual Paulista, Fac Med Vet & Zootecnia, Botucatu, SP, BrazilUniv Estadual Paulista, Inst Biociencias, Botucatu, SP, BrazilUniv Estadual Paulista, Fac Med Vet & Zootecnia, Botucatu, SP, BrazilUniv Estadual Paulista, Inst Biociencias, Botucatu, SP, BrazilArquivo Brasileiro Medicina Veterinaria ZootecniaUniversidade Estadual Paulista (Unesp)Perches, C. S. [UNESP]Pellizzon, C. H. [UNESP]Ranzani, J. J. T. [UNESP]Donatti, C.Padovani, C. R. [UNESP]Merlini, N. B. [UNESP]Fonzar, J. F.Beserra, H. E. O. [UNESP]Rocha, N. S. [UNESP]Brandao, C. V. S. [UNESP]2018-11-27T06:00:14Z2018-11-27T06:00:14Z2015-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1607-1615application/pdfhttp://dx.doi.org/10.1590/1678-4162-8142Arquivo Brasileiro De Medicina Veterinaria E Zootecnia. Minas Gerais: Arquivo Brasileiro Medicina Veterinaria Zootecnia, v. 67, n. 6, p. 1607-1615, 2015.0102-0935http://hdl.handle.net/11449/16501210.1590/1678-4162-8142S0102-09352015000601607WOS:000367094600017S0102-09352015000601607.pdf001939377980106910876153890136550000-0002-4494-41800000-0002-2011-5214Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporArquivo Brasileiro De Medicina Veterinaria E Zootecnia0,248info:eu-repo/semantics/openAccess2024-01-10T06:23:26Zoai:repositorio.unesp.br:11449/165012Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-10T06:23:26Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Expression of matrix metalloproteinases and PCNA in deep corneal ulcers induced in rabbits, treated with platelet-rich plasma |
title |
Expression of matrix metalloproteinases and PCNA in deep corneal ulcers induced in rabbits, treated with platelet-rich plasma |
spellingShingle |
Expression of matrix metalloproteinases and PCNA in deep corneal ulcers induced in rabbits, treated with platelet-rich plasma Perches, C. S. [UNESP] platelet-poor plasma MMP PRP healing corneal |
title_short |
Expression of matrix metalloproteinases and PCNA in deep corneal ulcers induced in rabbits, treated with platelet-rich plasma |
title_full |
Expression of matrix metalloproteinases and PCNA in deep corneal ulcers induced in rabbits, treated with platelet-rich plasma |
title_fullStr |
Expression of matrix metalloproteinases and PCNA in deep corneal ulcers induced in rabbits, treated with platelet-rich plasma |
title_full_unstemmed |
Expression of matrix metalloproteinases and PCNA in deep corneal ulcers induced in rabbits, treated with platelet-rich plasma |
title_sort |
Expression of matrix metalloproteinases and PCNA in deep corneal ulcers induced in rabbits, treated with platelet-rich plasma |
author |
Perches, C. S. [UNESP] |
author_facet |
Perches, C. S. [UNESP] Pellizzon, C. H. [UNESP] Ranzani, J. J. T. [UNESP] Donatti, C. Padovani, C. R. [UNESP] Merlini, N. B. [UNESP] Fonzar, J. F. Beserra, H. E. O. [UNESP] Rocha, N. S. [UNESP] Brandao, C. V. S. [UNESP] |
author_role |
author |
author2 |
Pellizzon, C. H. [UNESP] Ranzani, J. J. T. [UNESP] Donatti, C. Padovani, C. R. [UNESP] Merlini, N. B. [UNESP] Fonzar, J. F. Beserra, H. E. O. [UNESP] Rocha, N. S. [UNESP] Brandao, C. V. S. [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Perches, C. S. [UNESP] Pellizzon, C. H. [UNESP] Ranzani, J. J. T. [UNESP] Donatti, C. Padovani, C. R. [UNESP] Merlini, N. B. [UNESP] Fonzar, J. F. Beserra, H. E. O. [UNESP] Rocha, N. S. [UNESP] Brandao, C. V. S. [UNESP] |
dc.subject.por.fl_str_mv |
platelet-poor plasma MMP PRP healing corneal |
topic |
platelet-poor plasma MMP PRP healing corneal |
description |
The aim of this study was to evaluate the influence of platelet-rich (PRP) and poor (PPP) plasma in cell proliferation and matrix metalloproteinases (MMPs) expression during the repair of deep corneal ulcers. Forty-five female rabbits were distributed in 3 experimental groups (G) (n = 15), referred to as PRP (GR), PPP (GP) and Control (GC) groups, in accordance with the treatment. All animals underwent surgical induction of unilateral corneal ulcer. PRP and PPP eye drops were made by using centrifuged blood through standardized protocol, and instilled five times a day. In GC, lubricant eye drops were used. Each group was subdivided (n = 5) according to the final time point, 4 (M4), 7 (M7) and 30 days (M30). The animals' corneas were processed for morphological and immunohistochemical analysis for PCNA, MMP1, MMP2, MMP9, MT1-MMP and TIMP1. In M4, the levels of MMP2 were higher in GP and GR, and in M7, this behavior was only observed in the GP. In M30, more epithelial cells and MMP1 expression were found in GR than GP. In GR, cell proliferation was higher in M4 than at other time points and MMP2 expression was higher in M4 than M30. The PRP stimulates cell proliferation in the early phase (M4) of treatment when compared to other time points, different from other treatments. The use of eye drops of platelet-rich and poor plasma influences the expression of matrix metalloproteinases involved in the corneal repair process. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-12-01 2018-11-27T06:00:14Z 2018-11-27T06:00:14Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1678-4162-8142 Arquivo Brasileiro De Medicina Veterinaria E Zootecnia. Minas Gerais: Arquivo Brasileiro Medicina Veterinaria Zootecnia, v. 67, n. 6, p. 1607-1615, 2015. 0102-0935 http://hdl.handle.net/11449/165012 10.1590/1678-4162-8142 S0102-09352015000601607 WOS:000367094600017 S0102-09352015000601607.pdf 0019393779801069 1087615389013655 0000-0002-4494-4180 0000-0002-2011-5214 |
url |
http://dx.doi.org/10.1590/1678-4162-8142 http://hdl.handle.net/11449/165012 |
identifier_str_mv |
Arquivo Brasileiro De Medicina Veterinaria E Zootecnia. Minas Gerais: Arquivo Brasileiro Medicina Veterinaria Zootecnia, v. 67, n. 6, p. 1607-1615, 2015. 0102-0935 10.1590/1678-4162-8142 S0102-09352015000601607 WOS:000367094600017 S0102-09352015000601607.pdf 0019393779801069 1087615389013655 0000-0002-4494-4180 0000-0002-2011-5214 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Arquivo Brasileiro De Medicina Veterinaria E Zootecnia 0,248 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1607-1615 application/pdf |
dc.publisher.none.fl_str_mv |
Arquivo Brasileiro Medicina Veterinaria Zootecnia |
publisher.none.fl_str_mv |
Arquivo Brasileiro Medicina Veterinaria Zootecnia |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799965574288113664 |