Detection and molecular characterisation of bovine Enterovirus in Brazil: Four decades since the first report
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1017/S0950268818003394 http://hdl.handle.net/11449/187456 |
Resumo: | It is suggested that bovine enteroviruses (BEV) are involved in the aetiology of enteric infections, respiratory disease, reproductive disorders and infertility. In this study, bovine faecal samples collected in different Brazilian states were subjected to RNA extraction, reverse transcription-polymerase chain reaction analysis and partial sequencing of the 5′-terminal portion of BEV. One hundred and three samples were tested with an overall positivity of 14.5%. Phylogenetic analysis clustered these BEV Brazilian samples into the Enterovirus F clade. Our results bring an important update of the virus presence in Brazil and contribute to a better understanding of the distribution and characterisation of BEV in cattle. |
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Detection and molecular characterisation of bovine Enterovirus in Brazil: Four decades since the first reportAnimal virusesBovine enterovirusCattle diseasesMolecular diagnosticsIt is suggested that bovine enteroviruses (BEV) are involved in the aetiology of enteric infections, respiratory disease, reproductive disorders and infertility. In this study, bovine faecal samples collected in different Brazilian states were subjected to RNA extraction, reverse transcription-polymerase chain reaction analysis and partial sequencing of the 5′-terminal portion of BEV. One hundred and three samples were tested with an overall positivity of 14.5%. Phylogenetic analysis clustered these BEV Brazilian samples into the Enterovirus F clade. Our results bring an important update of the virus presence in Brazil and contribute to a better understanding of the distribution and characterisation of BEV in cattle.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Veterinary Medicine Faculty of Animal Science and Food Engineering University of São Paulo (FZEA/USP), Avenue Duque de Caxias Norte, 225, Jardim EliteDepartment of Preventive Veterinary Medicine and Animal Reproduction São Paulo State University (UNESP), Access Route Prof. Paulo Donato CastellaniDepartment of Clinical Analysis Faculty of Pharmacy Alfenas Federal University (UNIFAL), Street Gabriel Monteiro da Silva, 700Department of Preventive Veterinary Medicine and Animal Reproduction São Paulo State University (UNESP), Access Route Prof. Paulo Donato CastellaniFAPESP: 2006/52060-3FAPESP: 2012/18441-0CNPq: 472509/2010-1Universidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Alfenas Federal University (UNIFAL)Candido, M.Almeida-Queiroz, S. R.Buzinaro, M. G. [UNESP]Livonesi, M. C.Fernandes, A. M.Sousa, R. L.M.2019-10-06T15:36:40Z2019-10-06T15:36:40Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1017/S0950268818003394Epidemiology and Infection, v. 147.1469-44090950-2688http://hdl.handle.net/11449/18745610.1017/S09502688180033942-s2.0-85062851062Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEpidemiology and Infectioninfo:eu-repo/semantics/openAccess2021-10-23T20:19:07Zoai:repositorio.unesp.br:11449/187456Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T20:19:07Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Detection and molecular characterisation of bovine Enterovirus in Brazil: Four decades since the first report |
title |
Detection and molecular characterisation of bovine Enterovirus in Brazil: Four decades since the first report |
spellingShingle |
Detection and molecular characterisation of bovine Enterovirus in Brazil: Four decades since the first report Candido, M. Animal viruses Bovine enterovirus Cattle diseases Molecular diagnostics |
title_short |
Detection and molecular characterisation of bovine Enterovirus in Brazil: Four decades since the first report |
title_full |
Detection and molecular characterisation of bovine Enterovirus in Brazil: Four decades since the first report |
title_fullStr |
Detection and molecular characterisation of bovine Enterovirus in Brazil: Four decades since the first report |
title_full_unstemmed |
Detection and molecular characterisation of bovine Enterovirus in Brazil: Four decades since the first report |
title_sort |
Detection and molecular characterisation of bovine Enterovirus in Brazil: Four decades since the first report |
author |
Candido, M. |
author_facet |
Candido, M. Almeida-Queiroz, S. R. Buzinaro, M. G. [UNESP] Livonesi, M. C. Fernandes, A. M. Sousa, R. L.M. |
author_role |
author |
author2 |
Almeida-Queiroz, S. R. Buzinaro, M. G. [UNESP] Livonesi, M. C. Fernandes, A. M. Sousa, R. L.M. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) Alfenas Federal University (UNIFAL) |
dc.contributor.author.fl_str_mv |
Candido, M. Almeida-Queiroz, S. R. Buzinaro, M. G. [UNESP] Livonesi, M. C. Fernandes, A. M. Sousa, R. L.M. |
dc.subject.por.fl_str_mv |
Animal viruses Bovine enterovirus Cattle diseases Molecular diagnostics |
topic |
Animal viruses Bovine enterovirus Cattle diseases Molecular diagnostics |
description |
It is suggested that bovine enteroviruses (BEV) are involved in the aetiology of enteric infections, respiratory disease, reproductive disorders and infertility. In this study, bovine faecal samples collected in different Brazilian states were subjected to RNA extraction, reverse transcription-polymerase chain reaction analysis and partial sequencing of the 5′-terminal portion of BEV. One hundred and three samples were tested with an overall positivity of 14.5%. Phylogenetic analysis clustered these BEV Brazilian samples into the Enterovirus F clade. Our results bring an important update of the virus presence in Brazil and contribute to a better understanding of the distribution and characterisation of BEV in cattle. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T15:36:40Z 2019-10-06T15:36:40Z 2019-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1017/S0950268818003394 Epidemiology and Infection, v. 147. 1469-4409 0950-2688 http://hdl.handle.net/11449/187456 10.1017/S0950268818003394 2-s2.0-85062851062 |
url |
http://dx.doi.org/10.1017/S0950268818003394 http://hdl.handle.net/11449/187456 |
identifier_str_mv |
Epidemiology and Infection, v. 147. 1469-4409 0950-2688 10.1017/S0950268818003394 2-s2.0-85062851062 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Epidemiology and Infection |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797789913031114752 |