Follicular environment as a predictive tool for embryo development and kinetics in cattle
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1071/RD18143 http://hdl.handle.net/11449/188267 |
Resumo: | Follicular fluid composition and the transcription pattern of granulosa cells were analysed to better comprehend associations between embryo development and morphokinetics. Bovine follicles were punctured and their respective follicular fluid and granulosa cells were collected. Cumulus-oocyte complexes derived from these follicles were matured and fertilised in vitro. Embryo morphology and kinetics were evaluated at 40 h after insemination, when embryos were classified as fast (FCL, four or more cells), slow (SCL, 2-3 cells) or non-cleaved (NCL). Their development was followed until the blastocyst stage. Glucose, pyruvate, cholesterol and oestradiol were quantified in the follicular fluid and the transcription pattern of 96 target genes was evaluated in granulosa cells by large-scale quantitative reverse transcription polymerase chain reaction. Follicular fluid from the blastocyst group had increased levels of glucose, total cholesterol and pyruvate compared to the non-blastocyst group, whereas higher levels of oestradiol were observed in the follicular fluid of embryos and blastocysts with fast cleavage. The transcriptional pattern revealed altered metabolic pathways between groups, such as lipid metabolism, cellular stress and cell signalling. In conclusion, both follicular fluid and granulosa cells are associated with the possibility of identifying follicles that may generate embryos with high potential to properly develop to the blastocyst stage. |
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Follicular environment as a predictive tool for embryo development and kinetics in cattlebovinefollicular fluidgranulosa cellsmorphologysystem biologyFollicular fluid composition and the transcription pattern of granulosa cells were analysed to better comprehend associations between embryo development and morphokinetics. Bovine follicles were punctured and their respective follicular fluid and granulosa cells were collected. Cumulus-oocyte complexes derived from these follicles were matured and fertilised in vitro. Embryo morphology and kinetics were evaluated at 40 h after insemination, when embryos were classified as fast (FCL, four or more cells), slow (SCL, 2-3 cells) or non-cleaved (NCL). Their development was followed until the blastocyst stage. Glucose, pyruvate, cholesterol and oestradiol were quantified in the follicular fluid and the transcription pattern of 96 target genes was evaluated in granulosa cells by large-scale quantitative reverse transcription polymerase chain reaction. Follicular fluid from the blastocyst group had increased levels of glucose, total cholesterol and pyruvate compared to the non-blastocyst group, whereas higher levels of oestradiol were observed in the follicular fluid of embryos and blastocysts with fast cleavage. The transcriptional pattern revealed altered metabolic pathways between groups, such as lipid metabolism, cellular stress and cell signalling. In conclusion, both follicular fluid and granulosa cells are associated with the possibility of identifying follicles that may generate embryos with high potential to properly develop to the blastocyst stage.Laboratory of Cellular and Molecular Biology Centre of Natural Sciences and Humanities Federal University of ABC, Av dos Estados, 5005, CEPLaboratorio para Investigaciones Biomédicas Escuela Superior Politécnica Del Litoral ESPOLInstitute of Biosciences Universidade Estadual Paulista (UNESP) Campus BotucatuDepartment of Biological Sciences School of Sciences and Languages Universidade Estadual Paulista (UNESP) Campus AssisDepartment of Animal Reproduction Faculty of Veterinary Medicine University of São PauloInstitute of Biosciences Universidade Estadual Paulista (UNESP) Campus BotucatuDepartment of Biological Sciences School of Sciences and Languages Universidade Estadual Paulista (UNESP) Campus AssisFederal University of ABCESPOLUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Alves, Gláucia PereiraCordeiro, Fernanda BertuccezBruna De Lima, CamilaAnnes, KellyCristina Dos Santos, ÉrikaIspada, JéssicaFontes, Patrícia Kubo [UNESP]Nogueira, Marcelo Fabio Gouveia [UNESP]Nichi, MarcílioMilazzotto, Marcella Pecora2019-10-06T16:02:37Z2019-10-06T16:02:37Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article451-461http://dx.doi.org/10.1071/RD18143Reproduction, Fertility and Development, v. 31, n. 3, p. 451-461, 2019.1448-59901031-3613http://hdl.handle.net/11449/18826710.1071/RD181432-s2.0-85055585910Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengReproduction, Fertility and Developmentinfo:eu-repo/semantics/openAccess2021-10-23T01:35:32Zoai:repositorio.unesp.br:11449/188267Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T01:35:32Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Follicular environment as a predictive tool for embryo development and kinetics in cattle |
title |
Follicular environment as a predictive tool for embryo development and kinetics in cattle |
spellingShingle |
Follicular environment as a predictive tool for embryo development and kinetics in cattle Alves, Gláucia Pereira bovine follicular fluid granulosa cells morphology system biology |
title_short |
Follicular environment as a predictive tool for embryo development and kinetics in cattle |
title_full |
Follicular environment as a predictive tool for embryo development and kinetics in cattle |
title_fullStr |
Follicular environment as a predictive tool for embryo development and kinetics in cattle |
title_full_unstemmed |
Follicular environment as a predictive tool for embryo development and kinetics in cattle |
title_sort |
Follicular environment as a predictive tool for embryo development and kinetics in cattle |
author |
Alves, Gláucia Pereira |
author_facet |
Alves, Gláucia Pereira Cordeiro, Fernanda Bertuccez Bruna De Lima, Camila Annes, Kelly Cristina Dos Santos, Érika Ispada, Jéssica Fontes, Patrícia Kubo [UNESP] Nogueira, Marcelo Fabio Gouveia [UNESP] Nichi, Marcílio Milazzotto, Marcella Pecora |
author_role |
author |
author2 |
Cordeiro, Fernanda Bertuccez Bruna De Lima, Camila Annes, Kelly Cristina Dos Santos, Érika Ispada, Jéssica Fontes, Patrícia Kubo [UNESP] Nogueira, Marcelo Fabio Gouveia [UNESP] Nichi, Marcílio Milazzotto, Marcella Pecora |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Federal University of ABC ESPOL Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Alves, Gláucia Pereira Cordeiro, Fernanda Bertuccez Bruna De Lima, Camila Annes, Kelly Cristina Dos Santos, Érika Ispada, Jéssica Fontes, Patrícia Kubo [UNESP] Nogueira, Marcelo Fabio Gouveia [UNESP] Nichi, Marcílio Milazzotto, Marcella Pecora |
dc.subject.por.fl_str_mv |
bovine follicular fluid granulosa cells morphology system biology |
topic |
bovine follicular fluid granulosa cells morphology system biology |
description |
Follicular fluid composition and the transcription pattern of granulosa cells were analysed to better comprehend associations between embryo development and morphokinetics. Bovine follicles were punctured and their respective follicular fluid and granulosa cells were collected. Cumulus-oocyte complexes derived from these follicles were matured and fertilised in vitro. Embryo morphology and kinetics were evaluated at 40 h after insemination, when embryos were classified as fast (FCL, four or more cells), slow (SCL, 2-3 cells) or non-cleaved (NCL). Their development was followed until the blastocyst stage. Glucose, pyruvate, cholesterol and oestradiol were quantified in the follicular fluid and the transcription pattern of 96 target genes was evaluated in granulosa cells by large-scale quantitative reverse transcription polymerase chain reaction. Follicular fluid from the blastocyst group had increased levels of glucose, total cholesterol and pyruvate compared to the non-blastocyst group, whereas higher levels of oestradiol were observed in the follicular fluid of embryos and blastocysts with fast cleavage. The transcriptional pattern revealed altered metabolic pathways between groups, such as lipid metabolism, cellular stress and cell signalling. In conclusion, both follicular fluid and granulosa cells are associated with the possibility of identifying follicles that may generate embryos with high potential to properly develop to the blastocyst stage. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T16:02:37Z 2019-10-06T16:02:37Z 2019-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1071/RD18143 Reproduction, Fertility and Development, v. 31, n. 3, p. 451-461, 2019. 1448-5990 1031-3613 http://hdl.handle.net/11449/188267 10.1071/RD18143 2-s2.0-85055585910 |
url |
http://dx.doi.org/10.1071/RD18143 http://hdl.handle.net/11449/188267 |
identifier_str_mv |
Reproduction, Fertility and Development, v. 31, n. 3, p. 451-461, 2019. 1448-5990 1031-3613 10.1071/RD18143 2-s2.0-85055585910 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Reproduction, Fertility and Development |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
451-461 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797789440409600000 |