Distribution of the stx1 and stx2 genes in Escherichia coli isolated from milk cattle according to season, age, and production scale in southwestern region of Goias, Brazil
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/1678-4162-9861 http://hdl.handle.net/11449/186332 |
Resumo: | This study determined the distribution of stx1 and stx2 genes in Escherichia coli isolated from dairy herds with regard to animal age, season, and farm production-scale, and analyzed the phylogenetic distribution of the groups A, B1, B2, and D of 276 isolates of bovine feces Shiga toxin-producing E. coli (STEC). The stx1 profile was the most common, detected in 20.4% (202/990) of the isolates, followed by stx2 (4.54%, 45/990) and stx1+stx2 (2.92%, 29/990). The stx1 gene was detected more frequently in calves than in adult animals. In the dry season (winter), the presence of stx1+stx2 profile in cattle feces was higher than in the rainy season (summer), while no significant changes were observed between seasons for the stx1 and stx2 profiles. The most predominant phylogenetic groups in adult animals were B1, A, and D, while groups A and B1 prevailed in calves. Our data highlight the importance of identifying STEC reservoirs, since 7.5% of the tested isolates were positive for stx2, the main profile responsible for the hemolytic-uremic syndrome (HUS). Moreover, these microorganisms are adapted to survive even in hostile environments and can contaminate the food production chain, posing a significant risk to consumers of animal products. |
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Distribution of the stx1 and stx2 genes in Escherichia coli isolated from milk cattle according to season, age, and production scale in southwestern region of Goias, BrazilShiga toxin-producing Escherichia coli (STEC)phylogenetic STEC groupsSTEC reservoirsStx1Stx2This study determined the distribution of stx1 and stx2 genes in Escherichia coli isolated from dairy herds with regard to animal age, season, and farm production-scale, and analyzed the phylogenetic distribution of the groups A, B1, B2, and D of 276 isolates of bovine feces Shiga toxin-producing E. coli (STEC). The stx1 profile was the most common, detected in 20.4% (202/990) of the isolates, followed by stx2 (4.54%, 45/990) and stx1+stx2 (2.92%, 29/990). The stx1 gene was detected more frequently in calves than in adult animals. In the dry season (winter), the presence of stx1+stx2 profile in cattle feces was higher than in the rainy season (summer), while no significant changes were observed between seasons for the stx1 and stx2 profiles. The most predominant phylogenetic groups in adult animals were B1, A, and D, while groups A and B1 prevailed in calves. Our data highlight the importance of identifying STEC reservoirs, since 7.5% of the tested isolates were positive for stx2, the main profile responsible for the hemolytic-uremic syndrome (HUS). Moreover, these microorganisms are adapted to survive even in hostile environments and can contaminate the food production chain, posing a significant risk to consumers of animal products.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Fed Goias, Jatai, GO, BrazilUniv Fed Pelotas, Pelotas, RS, BrazilUniv Sao Paulo, Sao Paulo, SP, BrazilUniv Estadual Paulista, Jaboticabal, SP, BrazilUniv Estadual Paulista, Jaboticabal, SP, BrazilCNPq: 503886/2009-2Arquivo Brasileiro Medicina Veterinaria ZootecniaUniversidade Federal de Goiás (UFG)Univ Fed PelotasUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Ferreira, M. R. A.Stella, A. E.Freitas-Filho, E. G.Silva, T. S.Nascimento, K. A. [UNESP]Pinto, J. F. N.Dias, M.Moreira, C. N.2019-10-04T18:54:14Z2019-10-04T18:54:14Z2018-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1807-1813application/pdfhttp://dx.doi.org/10.1590/1678-4162-9861Arquivo Brasileiro De Medicina Veterinaria E Zootecnia. Minas Gerais: Arquivo Brasileiro Medicina Veterinaria Zootecnia, v. 70, n. 6, p. 1807-1813, 2018.0102-0935http://hdl.handle.net/11449/18633210.1590/1678-4162-9861S0102-09352018000601807WOS:000452984200020S0102-09352018000601807.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArquivo Brasileiro De Medicina Veterinaria E Zootecniainfo:eu-repo/semantics/openAccess2023-11-27T06:14:07Zoai:repositorio.unesp.br:11449/186332Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-27T06:14:07Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Distribution of the stx1 and stx2 genes in Escherichia coli isolated from milk cattle according to season, age, and production scale in southwestern region of Goias, Brazil |
title |
Distribution of the stx1 and stx2 genes in Escherichia coli isolated from milk cattle according to season, age, and production scale in southwestern region of Goias, Brazil |
spellingShingle |
Distribution of the stx1 and stx2 genes in Escherichia coli isolated from milk cattle according to season, age, and production scale in southwestern region of Goias, Brazil Ferreira, M. R. A. Shiga toxin-producing Escherichia coli (STEC) phylogenetic STEC groups STEC reservoirs Stx1 Stx2 |
title_short |
Distribution of the stx1 and stx2 genes in Escherichia coli isolated from milk cattle according to season, age, and production scale in southwestern region of Goias, Brazil |
title_full |
Distribution of the stx1 and stx2 genes in Escherichia coli isolated from milk cattle according to season, age, and production scale in southwestern region of Goias, Brazil |
title_fullStr |
Distribution of the stx1 and stx2 genes in Escherichia coli isolated from milk cattle according to season, age, and production scale in southwestern region of Goias, Brazil |
title_full_unstemmed |
Distribution of the stx1 and stx2 genes in Escherichia coli isolated from milk cattle according to season, age, and production scale in southwestern region of Goias, Brazil |
title_sort |
Distribution of the stx1 and stx2 genes in Escherichia coli isolated from milk cattle according to season, age, and production scale in southwestern region of Goias, Brazil |
author |
Ferreira, M. R. A. |
author_facet |
Ferreira, M. R. A. Stella, A. E. Freitas-Filho, E. G. Silva, T. S. Nascimento, K. A. [UNESP] Pinto, J. F. N. Dias, M. Moreira, C. N. |
author_role |
author |
author2 |
Stella, A. E. Freitas-Filho, E. G. Silva, T. S. Nascimento, K. A. [UNESP] Pinto, J. F. N. Dias, M. Moreira, C. N. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Goiás (UFG) Univ Fed Pelotas Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Ferreira, M. R. A. Stella, A. E. Freitas-Filho, E. G. Silva, T. S. Nascimento, K. A. [UNESP] Pinto, J. F. N. Dias, M. Moreira, C. N. |
dc.subject.por.fl_str_mv |
Shiga toxin-producing Escherichia coli (STEC) phylogenetic STEC groups STEC reservoirs Stx1 Stx2 |
topic |
Shiga toxin-producing Escherichia coli (STEC) phylogenetic STEC groups STEC reservoirs Stx1 Stx2 |
description |
This study determined the distribution of stx1 and stx2 genes in Escherichia coli isolated from dairy herds with regard to animal age, season, and farm production-scale, and analyzed the phylogenetic distribution of the groups A, B1, B2, and D of 276 isolates of bovine feces Shiga toxin-producing E. coli (STEC). The stx1 profile was the most common, detected in 20.4% (202/990) of the isolates, followed by stx2 (4.54%, 45/990) and stx1+stx2 (2.92%, 29/990). The stx1 gene was detected more frequently in calves than in adult animals. In the dry season (winter), the presence of stx1+stx2 profile in cattle feces was higher than in the rainy season (summer), while no significant changes were observed between seasons for the stx1 and stx2 profiles. The most predominant phylogenetic groups in adult animals were B1, A, and D, while groups A and B1 prevailed in calves. Our data highlight the importance of identifying STEC reservoirs, since 7.5% of the tested isolates were positive for stx2, the main profile responsible for the hemolytic-uremic syndrome (HUS). Moreover, these microorganisms are adapted to survive even in hostile environments and can contaminate the food production chain, posing a significant risk to consumers of animal products. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-11-01 2019-10-04T18:54:14Z 2019-10-04T18:54:14Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1678-4162-9861 Arquivo Brasileiro De Medicina Veterinaria E Zootecnia. Minas Gerais: Arquivo Brasileiro Medicina Veterinaria Zootecnia, v. 70, n. 6, p. 1807-1813, 2018. 0102-0935 http://hdl.handle.net/11449/186332 10.1590/1678-4162-9861 S0102-09352018000601807 WOS:000452984200020 S0102-09352018000601807.pdf |
url |
http://dx.doi.org/10.1590/1678-4162-9861 http://hdl.handle.net/11449/186332 |
identifier_str_mv |
Arquivo Brasileiro De Medicina Veterinaria E Zootecnia. Minas Gerais: Arquivo Brasileiro Medicina Veterinaria Zootecnia, v. 70, n. 6, p. 1807-1813, 2018. 0102-0935 10.1590/1678-4162-9861 S0102-09352018000601807 WOS:000452984200020 S0102-09352018000601807.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Arquivo Brasileiro De Medicina Veterinaria E Zootecnia |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1807-1813 application/pdf |
dc.publisher.none.fl_str_mv |
Arquivo Brasileiro Medicina Veterinaria Zootecnia |
publisher.none.fl_str_mv |
Arquivo Brasileiro Medicina Veterinaria Zootecnia |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799965088136822784 |