Inhibition of Nitric Oxide and Tumour Necrosis Factor-alpha Production in Peritoneal Macrophages by Aspergillus nidulans Melanin
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1248/bpb.b13-00445 http://hdl.handle.net/11449/113439 |
Resumo: | The naturally occurring pigment, melanin is found in organisms of all phylogenetic kingdoms, including fungi, and exhibits a wide range of biological activities. Our objective was to investigate the effects of melanin extracted from the fungus Aspergillus nidulans on the production of the pro-inflammatory mediators nitric oxide (NO) and tumour necrosis factor-alpha (TNF-alpha) in peritoneal macrophages and on the viability of McCoy mouse fibroblasts. The results showed that A. nidulans melanin did not stimulate NO production in macrophages, but it inhibited the NO production in lipopolysaccharide (LPS)-stimulated macrophages by approximately 82%. Similarly, A. nidulans melanin inhibited LPS-stimulated TNF-alpha production by 52% and showed a slight stimulatory effect on TNF-alpha production in macrophages. In addition, the toxicity of A. nidulans melanin to McCoy cells was much lesser (IC50=373.5 +/- 2.4 mu g/mL) than that of known agents such as cisplatin (IC50=41.2 mu g/mL). The viability of peritoneal macrophages was greater than 90% at the highest melanin concentration tested (100 mu g/mL). Thus, the combination of low cytotoxicity and marked inhibition of TNF-alpha and NO production suggests that A. nidulans melanin has potential as an anti-inflammatory agent and may be used in the future for development of new drugs with therapeutic utility. |
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Inhibition of Nitric Oxide and Tumour Necrosis Factor-alpha Production in Peritoneal Macrophages by Aspergillus nidulans MelaninAspergillus nidulansmelaninnitric oxidetumour necrosis factor-alphafungusThe naturally occurring pigment, melanin is found in organisms of all phylogenetic kingdoms, including fungi, and exhibits a wide range of biological activities. Our objective was to investigate the effects of melanin extracted from the fungus Aspergillus nidulans on the production of the pro-inflammatory mediators nitric oxide (NO) and tumour necrosis factor-alpha (TNF-alpha) in peritoneal macrophages and on the viability of McCoy mouse fibroblasts. The results showed that A. nidulans melanin did not stimulate NO production in macrophages, but it inhibited the NO production in lipopolysaccharide (LPS)-stimulated macrophages by approximately 82%. Similarly, A. nidulans melanin inhibited LPS-stimulated TNF-alpha production by 52% and showed a slight stimulatory effect on TNF-alpha production in macrophages. In addition, the toxicity of A. nidulans melanin to McCoy cells was much lesser (IC50=373.5 +/- 2.4 mu g/mL) than that of known agents such as cisplatin (IC50=41.2 mu g/mL). The viability of peritoneal macrophages was greater than 90% at the highest melanin concentration tested (100 mu g/mL). Thus, the combination of low cytotoxicity and marked inhibition of TNF-alpha and NO production suggests that A. nidulans melanin has potential as an anti-inflammatory agent and may be used in the future for development of new drugs with therapeutic utility.Programa de Apoio a Pos-graduacao (PROAP-UNESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Fed Espirito Santo, Dept Pharmaceut Sci, BR-29040090 Vitoria, ES, BrazilSao Paulo State Univ UNESP, Fac Pharmaceut Sci, Dept Clin Anal, BR-14801902 Araraquara, SP, BrazilSao Paulo State Univ UNESP, Inst Chem, Dept Biochem & Technol Chem, BR-14801970 Araraquara, SP, BrazilSao Paulo State Univ UNESP, Fac Pharmaceut Sci, Dept Clin Anal, BR-14801902 Araraquara, SP, BrazilSao Paulo State Univ UNESP, Inst Chem, Dept Biochem & Technol Chem, BR-14801970 Araraquara, SP, BrazilPharmaceutical Soc JapanUniversidade Federal do Espírito Santo (UFES)Universidade Estadual Paulista (Unesp)Ribeiro Goncalves, Rita de CassiaKitagawa, Rodrigo RezendeGoncalves Raddi, Maria Stella [UNESP]Carlos, Iracilda Zeppone [UNESP]Pombeiro-Sponchiado, Sandra Regina2014-12-03T13:11:42Z2014-12-03T13:11:42Z2013-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1915-1920application/pdfhttp://dx.doi.org/10.1248/bpb.b13-00445Biological & Pharmaceutical Bulletin. Tokyo: Pharmaceutical Soc Japan, v. 36, n. 12, p. 1915-1920, 2013.0918-6158http://hdl.handle.net/11449/113439WOS:000327573800006WOS000327573800006.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiological & Pharmaceutical Bulletin1.6940,626info:eu-repo/semantics/openAccess2024-01-07T06:22:17Zoai:repositorio.unesp.br:11449/113439Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-07T06:22:17Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Inhibition of Nitric Oxide and Tumour Necrosis Factor-alpha Production in Peritoneal Macrophages by Aspergillus nidulans Melanin |
title |
Inhibition of Nitric Oxide and Tumour Necrosis Factor-alpha Production in Peritoneal Macrophages by Aspergillus nidulans Melanin |
spellingShingle |
Inhibition of Nitric Oxide and Tumour Necrosis Factor-alpha Production in Peritoneal Macrophages by Aspergillus nidulans Melanin Ribeiro Goncalves, Rita de Cassia Aspergillus nidulans melanin nitric oxide tumour necrosis factor-alpha fungus |
title_short |
Inhibition of Nitric Oxide and Tumour Necrosis Factor-alpha Production in Peritoneal Macrophages by Aspergillus nidulans Melanin |
title_full |
Inhibition of Nitric Oxide and Tumour Necrosis Factor-alpha Production in Peritoneal Macrophages by Aspergillus nidulans Melanin |
title_fullStr |
Inhibition of Nitric Oxide and Tumour Necrosis Factor-alpha Production in Peritoneal Macrophages by Aspergillus nidulans Melanin |
title_full_unstemmed |
Inhibition of Nitric Oxide and Tumour Necrosis Factor-alpha Production in Peritoneal Macrophages by Aspergillus nidulans Melanin |
title_sort |
Inhibition of Nitric Oxide and Tumour Necrosis Factor-alpha Production in Peritoneal Macrophages by Aspergillus nidulans Melanin |
author |
Ribeiro Goncalves, Rita de Cassia |
author_facet |
Ribeiro Goncalves, Rita de Cassia Kitagawa, Rodrigo Rezende Goncalves Raddi, Maria Stella [UNESP] Carlos, Iracilda Zeppone [UNESP] Pombeiro-Sponchiado, Sandra Regina |
author_role |
author |
author2 |
Kitagawa, Rodrigo Rezende Goncalves Raddi, Maria Stella [UNESP] Carlos, Iracilda Zeppone [UNESP] Pombeiro-Sponchiado, Sandra Regina |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal do Espírito Santo (UFES) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Ribeiro Goncalves, Rita de Cassia Kitagawa, Rodrigo Rezende Goncalves Raddi, Maria Stella [UNESP] Carlos, Iracilda Zeppone [UNESP] Pombeiro-Sponchiado, Sandra Regina |
dc.subject.por.fl_str_mv |
Aspergillus nidulans melanin nitric oxide tumour necrosis factor-alpha fungus |
topic |
Aspergillus nidulans melanin nitric oxide tumour necrosis factor-alpha fungus |
description |
The naturally occurring pigment, melanin is found in organisms of all phylogenetic kingdoms, including fungi, and exhibits a wide range of biological activities. Our objective was to investigate the effects of melanin extracted from the fungus Aspergillus nidulans on the production of the pro-inflammatory mediators nitric oxide (NO) and tumour necrosis factor-alpha (TNF-alpha) in peritoneal macrophages and on the viability of McCoy mouse fibroblasts. The results showed that A. nidulans melanin did not stimulate NO production in macrophages, but it inhibited the NO production in lipopolysaccharide (LPS)-stimulated macrophages by approximately 82%. Similarly, A. nidulans melanin inhibited LPS-stimulated TNF-alpha production by 52% and showed a slight stimulatory effect on TNF-alpha production in macrophages. In addition, the toxicity of A. nidulans melanin to McCoy cells was much lesser (IC50=373.5 +/- 2.4 mu g/mL) than that of known agents such as cisplatin (IC50=41.2 mu g/mL). The viability of peritoneal macrophages was greater than 90% at the highest melanin concentration tested (100 mu g/mL). Thus, the combination of low cytotoxicity and marked inhibition of TNF-alpha and NO production suggests that A. nidulans melanin has potential as an anti-inflammatory agent and may be used in the future for development of new drugs with therapeutic utility. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-12-01 2014-12-03T13:11:42Z 2014-12-03T13:11:42Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1248/bpb.b13-00445 Biological & Pharmaceutical Bulletin. Tokyo: Pharmaceutical Soc Japan, v. 36, n. 12, p. 1915-1920, 2013. 0918-6158 http://hdl.handle.net/11449/113439 WOS:000327573800006 WOS000327573800006.pdf |
url |
http://dx.doi.org/10.1248/bpb.b13-00445 http://hdl.handle.net/11449/113439 |
identifier_str_mv |
Biological & Pharmaceutical Bulletin. Tokyo: Pharmaceutical Soc Japan, v. 36, n. 12, p. 1915-1920, 2013. 0918-6158 WOS:000327573800006 WOS000327573800006.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biological & Pharmaceutical Bulletin 1.694 0,626 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1915-1920 application/pdf |
dc.publisher.none.fl_str_mv |
Pharmaceutical Soc Japan |
publisher.none.fl_str_mv |
Pharmaceutical Soc Japan |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1797790223681191936 |