Immunocytochemical Analysis of the Wall Ingrowths in the Digestive Gland Transfer Cells in Aldrovanda vesiculosa L. (Droseraceae)
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/cells11142218 http://hdl.handle.net/11449/240572 |
Resumo: | Carnivorous plants are unique due to their ability to attract small animals or protozoa, retain them in specialized traps, digest them, and absorb nutrients from the dissolved prey material; however, to this end, these plants need a special secretion-digestive system (glands). A common trait of the digestive glands of carnivorous plants is the presence of transfer cells. Using the aquatic carnivorous species Aldrovanda vesiculosa, we showed carnivorous plants as a model for studies of wall ingrowths/transfer cells. We addressed the following questions: Is the cell wall ingrowth composition the same between carnivorous plant glands and other plant system models? Is there a difference in the cell wall ingrowth composition between various types of gland cells (glandular versus endodermoid cells)? Fluorescence microscopy and immunogold electron microscopy were employed to localize carbohydrate epitopes associated with major cell wall polysaccharides and glycoproteins. The cell wall ingrowths were enriched with arabinogalactan proteins (AGPs) localized with the JIM8, JIM13, and JIM14 epitopes. Both methylesterified and de-esterified homogalacturonans (HGs) were absent or weakly present in the wall ingrowths in transfer cells (stalk cells and head cells of the gland). Both the cell walls and the cell wall ingrowths in the transfer cells were rich in hemicelluloses: xyloglucan (LM15) and galactoxyloglucan (LM25). There were differences in the composition between the cell wall ingrowths and the primary cell walls in A. vesiculosa secretory gland cells in the case of the absence or inaccessibility of pectins (JIM5, LM19, JIM7, LM5, LM6 epitopes); thus, the wall ingrowths are specific cell wall microdomains. Even in the same organ (gland), transfer cells may differ in the composition of the cell wall ingrowths (glandular versus endodermoid cells). We found both similarities and differences in the composition of the cell wall ingrowths between the A. vesiculosa transfer cells and transfer cells of other plant species. |
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Immunocytochemical Analysis of the Wall Ingrowths in the Digestive Gland Transfer Cells in Aldrovanda vesiculosa L. (Droseraceae)arabinogalactan proteinscarnivorous plantscell wallDroseraceaetransfer cellswall ingrowthswall labyrinthwaterwheel plantCarnivorous plants are unique due to their ability to attract small animals or protozoa, retain them in specialized traps, digest them, and absorb nutrients from the dissolved prey material; however, to this end, these plants need a special secretion-digestive system (glands). A common trait of the digestive glands of carnivorous plants is the presence of transfer cells. Using the aquatic carnivorous species Aldrovanda vesiculosa, we showed carnivorous plants as a model for studies of wall ingrowths/transfer cells. We addressed the following questions: Is the cell wall ingrowth composition the same between carnivorous plant glands and other plant system models? Is there a difference in the cell wall ingrowth composition between various types of gland cells (glandular versus endodermoid cells)? Fluorescence microscopy and immunogold electron microscopy were employed to localize carbohydrate epitopes associated with major cell wall polysaccharides and glycoproteins. The cell wall ingrowths were enriched with arabinogalactan proteins (AGPs) localized with the JIM8, JIM13, and JIM14 epitopes. Both methylesterified and de-esterified homogalacturonans (HGs) were absent or weakly present in the wall ingrowths in transfer cells (stalk cells and head cells of the gland). Both the cell walls and the cell wall ingrowths in the transfer cells were rich in hemicelluloses: xyloglucan (LM15) and galactoxyloglucan (LM25). There were differences in the composition between the cell wall ingrowths and the primary cell walls in A. vesiculosa secretory gland cells in the case of the absence or inaccessibility of pectins (JIM5, LM19, JIM7, LM5, LM6 epitopes); thus, the wall ingrowths are specific cell wall microdomains. Even in the same organ (gland), transfer cells may differ in the composition of the cell wall ingrowths (glandular versus endodermoid cells). We found both similarities and differences in the composition of the cell wall ingrowths between the A. vesiculosa transfer cells and transfer cells of other plant species.Department of Plant Cytology and Embryology Institute of Botany Faculty of Biology Jagiellonian University in Kraków, 9 Gronostajowa StDepartment of Plant Cytology and Embryology Faculty of Biology University of Gdańsk, 59 Wita Stwosza StDepartment of Botany Physiology and Plant Protection Faculty of Biotechnology and Horticulture University of Agriculture in Kraków, 29 Listopada 54 AveInstitute of Biology Biotechnology and Environmental Protection Faculty of Natural Sciences University of Silesia in Katowice, 9 Bankowa StDepartment of Analytical Chemistry Medical University of Lublin, Chodźki 4aLaboratory of Plant Systematics School of Agricultural and Veterinarian Sciences São Paulo State University (Unesp, CEPLaboratory of Plant Systematics School of Agricultural and Veterinarian Sciences São Paulo State University (Unesp, CEPJagiellonian University in KrakówUniversity of GdańskUniversity of Agriculture in KrakówUniversity of Silesia in KatowiceMedical University of LublinUniversidade Estadual Paulista (UNESP)Płachno, Bartosz J.Kapusta, MałgorzataStolarczyk, PiotrŚwiątek, PiotrStrzemski, MaciejMiranda, Vitor F. O. [UNESP]2023-03-01T20:23:15Z2023-03-01T20:23:15Z2022-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/cells11142218Cells, v. 11, n. 14, 2022.2073-4409http://hdl.handle.net/11449/24057210.3390/cells111422182-s2.0-85135120064Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCellsinfo:eu-repo/semantics/openAccess2023-03-01T20:23:15Zoai:repositorio.unesp.br:11449/240572Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-03-01T20:23:15Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Immunocytochemical Analysis of the Wall Ingrowths in the Digestive Gland Transfer Cells in Aldrovanda vesiculosa L. (Droseraceae) |
title |
Immunocytochemical Analysis of the Wall Ingrowths in the Digestive Gland Transfer Cells in Aldrovanda vesiculosa L. (Droseraceae) |
spellingShingle |
Immunocytochemical Analysis of the Wall Ingrowths in the Digestive Gland Transfer Cells in Aldrovanda vesiculosa L. (Droseraceae) Płachno, Bartosz J. arabinogalactan proteins carnivorous plants cell wall Droseraceae transfer cells wall ingrowths wall labyrinth waterwheel plant |
title_short |
Immunocytochemical Analysis of the Wall Ingrowths in the Digestive Gland Transfer Cells in Aldrovanda vesiculosa L. (Droseraceae) |
title_full |
Immunocytochemical Analysis of the Wall Ingrowths in the Digestive Gland Transfer Cells in Aldrovanda vesiculosa L. (Droseraceae) |
title_fullStr |
Immunocytochemical Analysis of the Wall Ingrowths in the Digestive Gland Transfer Cells in Aldrovanda vesiculosa L. (Droseraceae) |
title_full_unstemmed |
Immunocytochemical Analysis of the Wall Ingrowths in the Digestive Gland Transfer Cells in Aldrovanda vesiculosa L. (Droseraceae) |
title_sort |
Immunocytochemical Analysis of the Wall Ingrowths in the Digestive Gland Transfer Cells in Aldrovanda vesiculosa L. (Droseraceae) |
author |
Płachno, Bartosz J. |
author_facet |
Płachno, Bartosz J. Kapusta, Małgorzata Stolarczyk, Piotr Świątek, Piotr Strzemski, Maciej Miranda, Vitor F. O. [UNESP] |
author_role |
author |
author2 |
Kapusta, Małgorzata Stolarczyk, Piotr Świątek, Piotr Strzemski, Maciej Miranda, Vitor F. O. [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Jagiellonian University in Kraków University of Gdańsk University of Agriculture in Kraków University of Silesia in Katowice Medical University of Lublin Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Płachno, Bartosz J. Kapusta, Małgorzata Stolarczyk, Piotr Świątek, Piotr Strzemski, Maciej Miranda, Vitor F. O. [UNESP] |
dc.subject.por.fl_str_mv |
arabinogalactan proteins carnivorous plants cell wall Droseraceae transfer cells wall ingrowths wall labyrinth waterwheel plant |
topic |
arabinogalactan proteins carnivorous plants cell wall Droseraceae transfer cells wall ingrowths wall labyrinth waterwheel plant |
description |
Carnivorous plants are unique due to their ability to attract small animals or protozoa, retain them in specialized traps, digest them, and absorb nutrients from the dissolved prey material; however, to this end, these plants need a special secretion-digestive system (glands). A common trait of the digestive glands of carnivorous plants is the presence of transfer cells. Using the aquatic carnivorous species Aldrovanda vesiculosa, we showed carnivorous plants as a model for studies of wall ingrowths/transfer cells. We addressed the following questions: Is the cell wall ingrowth composition the same between carnivorous plant glands and other plant system models? Is there a difference in the cell wall ingrowth composition between various types of gland cells (glandular versus endodermoid cells)? Fluorescence microscopy and immunogold electron microscopy were employed to localize carbohydrate epitopes associated with major cell wall polysaccharides and glycoproteins. The cell wall ingrowths were enriched with arabinogalactan proteins (AGPs) localized with the JIM8, JIM13, and JIM14 epitopes. Both methylesterified and de-esterified homogalacturonans (HGs) were absent or weakly present in the wall ingrowths in transfer cells (stalk cells and head cells of the gland). Both the cell walls and the cell wall ingrowths in the transfer cells were rich in hemicelluloses: xyloglucan (LM15) and galactoxyloglucan (LM25). There were differences in the composition between the cell wall ingrowths and the primary cell walls in A. vesiculosa secretory gland cells in the case of the absence or inaccessibility of pectins (JIM5, LM19, JIM7, LM5, LM6 epitopes); thus, the wall ingrowths are specific cell wall microdomains. Even in the same organ (gland), transfer cells may differ in the composition of the cell wall ingrowths (glandular versus endodermoid cells). We found both similarities and differences in the composition of the cell wall ingrowths between the A. vesiculosa transfer cells and transfer cells of other plant species. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-07-01 2023-03-01T20:23:15Z 2023-03-01T20:23:15Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/cells11142218 Cells, v. 11, n. 14, 2022. 2073-4409 http://hdl.handle.net/11449/240572 10.3390/cells11142218 2-s2.0-85135120064 |
url |
http://dx.doi.org/10.3390/cells11142218 http://hdl.handle.net/11449/240572 |
identifier_str_mv |
Cells, v. 11, n. 14, 2022. 2073-4409 10.3390/cells11142218 2-s2.0-85135120064 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Cells |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799965047284301824 |