Patterns of the innate immune response in tambaqui Colossoma macropomum: Modulation of gene expression in haemorrhagic septicaemia caused by Aeromonas hydrophila
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.micpath.2020.104638 http://hdl.handle.net/11449/207038 |
Resumo: | The production of tambaqui Colossoma macropomum has recently reached a milestone, being considered the main native species produced in South American continental waters. Despite the importance of this fish, its immunity is not well understood. In this study we established some patterns of innate immunity for the species via two experiments. Both studies evaluated the fish in the absence (intraperitoneal saline) or presence (intraperitoneal, 3 x 107 CFU/mL of Aeromonas hydrophila at 0.1 mL/10 g of living weight) of infection at 5 points over time-course of 14 days (0 h, 6 h, 24 h, 7 d, 14 d). In the first experiment, the partial gene sequences and gene expression of IL-1β, IRAK-1, C3, C4, lysozyme, IL-10, HSP70 and β-actin were determined in the main secondary lymphoid organs of fish: the spleen and head kidney. The second study was performed to analyse the alternative complement pathway ACH50 in serum to support the elucidation of C3 gene expression. Results of the gene expression assays showed a tendency towards up-regulation of immune genes in infected fish in early phases of infection (mostly around 6 h and 24 h) and in the chronic phase (7 d and 14 d), with the exception of HSP70 which showed a down-regulation in infected fish. Our results also suggested that lysozyme was evolved in both pro- and anti-inflammatory activities. For genes of the complement system, it was demonstrated that C4 regulation followed the tendency of pro-inflammatory genes. However, the C3 gene was, surprisingly, not expressed in most fish and this corroborated with the results of the complement system activity in serum that also did not show activity in most fish. The possible reasons for the regulation of gene expression and association with fish disease are addressed in this paper. |
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Patterns of the innate immune response in tambaqui Colossoma macropomum: Modulation of gene expression in haemorrhagic septicaemia caused by Aeromonas hydrophilaAeromonas hydrophilaAquacultureBlack pacuComplement systemInterleukinsLysozymeThe production of tambaqui Colossoma macropomum has recently reached a milestone, being considered the main native species produced in South American continental waters. Despite the importance of this fish, its immunity is not well understood. In this study we established some patterns of innate immunity for the species via two experiments. Both studies evaluated the fish in the absence (intraperitoneal saline) or presence (intraperitoneal, 3 x 107 CFU/mL of Aeromonas hydrophila at 0.1 mL/10 g of living weight) of infection at 5 points over time-course of 14 days (0 h, 6 h, 24 h, 7 d, 14 d). In the first experiment, the partial gene sequences and gene expression of IL-1β, IRAK-1, C3, C4, lysozyme, IL-10, HSP70 and β-actin were determined in the main secondary lymphoid organs of fish: the spleen and head kidney. The second study was performed to analyse the alternative complement pathway ACH50 in serum to support the elucidation of C3 gene expression. Results of the gene expression assays showed a tendency towards up-regulation of immune genes in infected fish in early phases of infection (mostly around 6 h and 24 h) and in the chronic phase (7 d and 14 d), with the exception of HSP70 which showed a down-regulation in infected fish. Our results also suggested that lysozyme was evolved in both pro- and anti-inflammatory activities. For genes of the complement system, it was demonstrated that C4 regulation followed the tendency of pro-inflammatory genes. However, the C3 gene was, surprisingly, not expressed in most fish and this corroborated with the results of the complement system activity in serum that also did not show activity in most fish. The possible reasons for the regulation of gene expression and association with fish disease are addressed in this paper.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Nilton Lins University (UNL) Laboratory of Microbiology Applied to Aquatic OrganismsNilton Lins University (UNL) Laboratory of Parasitology and Pathology of Aquatic OrganismsSão Paulo State University (UNESP) Aquaculture Center of UNESP Laboratory of Microbiology and Parasitology of Aquatic OrganismsSão Paulo State University (UNESP) Aquaculture Center of UNESP Laboratory of Aquaculture Genetics and ConservationWageningen University & Research (WUR) Aquaculture and Fisheries GroupUniversity of Passo Fundo (UPF) Faculty of Agronomy and Veterinary Medicine Laboratory of Microbiology and Advanced ImmunologySão Paulo State University (UNESP) Aquaculture Center of UNESP Laboratory of Microbiology and Parasitology of Aquatic OrganismsSão Paulo State University (UNESP) Aquaculture Center of UNESP Laboratory of Aquaculture Genetics and ConservationCAPES: 001CNPq: 305007/2016-5CNPq: 431713/2016-2Laboratory of Microbiology Applied to Aquatic OrganismsLaboratory of Parasitology and Pathology of Aquatic OrganismsUniversidade Estadual Paulista (Unesp)Aquaculture and Fisheries GroupLaboratory of Microbiology and Advanced ImmunologyGallani, Sílvia UmedaValladão, Gustavo Moraes RamosAlves, Lindomar de Oliveira [UNESP]Kotzent, Suzana [UNESP]Hashimoto, Diogo Teruo [UNESP]Wiegertjes, GeertKirsten, KarinaKreutz, Luiz CarlosPilarski, Fabiana [UNESP]2021-06-25T10:48:01Z2021-06-25T10:48:01Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.micpath.2020.104638Microbial Pathogenesis, v. 150.1096-12080882-4010http://hdl.handle.net/11449/20703810.1016/j.micpath.2020.1046382-s2.0-85098468750Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMicrobial Pathogenesisinfo:eu-repo/semantics/openAccess2024-04-09T15:37:02Zoai:repositorio.unesp.br:11449/207038Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-04-09T15:37:02Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Patterns of the innate immune response in tambaqui Colossoma macropomum: Modulation of gene expression in haemorrhagic septicaemia caused by Aeromonas hydrophila |
title |
Patterns of the innate immune response in tambaqui Colossoma macropomum: Modulation of gene expression in haemorrhagic septicaemia caused by Aeromonas hydrophila |
spellingShingle |
Patterns of the innate immune response in tambaqui Colossoma macropomum: Modulation of gene expression in haemorrhagic septicaemia caused by Aeromonas hydrophila Gallani, Sílvia Umeda Aeromonas hydrophila Aquaculture Black pacu Complement system Interleukins Lysozyme |
title_short |
Patterns of the innate immune response in tambaqui Colossoma macropomum: Modulation of gene expression in haemorrhagic septicaemia caused by Aeromonas hydrophila |
title_full |
Patterns of the innate immune response in tambaqui Colossoma macropomum: Modulation of gene expression in haemorrhagic septicaemia caused by Aeromonas hydrophila |
title_fullStr |
Patterns of the innate immune response in tambaqui Colossoma macropomum: Modulation of gene expression in haemorrhagic septicaemia caused by Aeromonas hydrophila |
title_full_unstemmed |
Patterns of the innate immune response in tambaqui Colossoma macropomum: Modulation of gene expression in haemorrhagic septicaemia caused by Aeromonas hydrophila |
title_sort |
Patterns of the innate immune response in tambaqui Colossoma macropomum: Modulation of gene expression in haemorrhagic septicaemia caused by Aeromonas hydrophila |
author |
Gallani, Sílvia Umeda |
author_facet |
Gallani, Sílvia Umeda Valladão, Gustavo Moraes Ramos Alves, Lindomar de Oliveira [UNESP] Kotzent, Suzana [UNESP] Hashimoto, Diogo Teruo [UNESP] Wiegertjes, Geert Kirsten, Karina Kreutz, Luiz Carlos Pilarski, Fabiana [UNESP] |
author_role |
author |
author2 |
Valladão, Gustavo Moraes Ramos Alves, Lindomar de Oliveira [UNESP] Kotzent, Suzana [UNESP] Hashimoto, Diogo Teruo [UNESP] Wiegertjes, Geert Kirsten, Karina Kreutz, Luiz Carlos Pilarski, Fabiana [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Laboratory of Microbiology Applied to Aquatic Organisms Laboratory of Parasitology and Pathology of Aquatic Organisms Universidade Estadual Paulista (Unesp) Aquaculture and Fisheries Group Laboratory of Microbiology and Advanced Immunology |
dc.contributor.author.fl_str_mv |
Gallani, Sílvia Umeda Valladão, Gustavo Moraes Ramos Alves, Lindomar de Oliveira [UNESP] Kotzent, Suzana [UNESP] Hashimoto, Diogo Teruo [UNESP] Wiegertjes, Geert Kirsten, Karina Kreutz, Luiz Carlos Pilarski, Fabiana [UNESP] |
dc.subject.por.fl_str_mv |
Aeromonas hydrophila Aquaculture Black pacu Complement system Interleukins Lysozyme |
topic |
Aeromonas hydrophila Aquaculture Black pacu Complement system Interleukins Lysozyme |
description |
The production of tambaqui Colossoma macropomum has recently reached a milestone, being considered the main native species produced in South American continental waters. Despite the importance of this fish, its immunity is not well understood. In this study we established some patterns of innate immunity for the species via two experiments. Both studies evaluated the fish in the absence (intraperitoneal saline) or presence (intraperitoneal, 3 x 107 CFU/mL of Aeromonas hydrophila at 0.1 mL/10 g of living weight) of infection at 5 points over time-course of 14 days (0 h, 6 h, 24 h, 7 d, 14 d). In the first experiment, the partial gene sequences and gene expression of IL-1β, IRAK-1, C3, C4, lysozyme, IL-10, HSP70 and β-actin were determined in the main secondary lymphoid organs of fish: the spleen and head kidney. The second study was performed to analyse the alternative complement pathway ACH50 in serum to support the elucidation of C3 gene expression. Results of the gene expression assays showed a tendency towards up-regulation of immune genes in infected fish in early phases of infection (mostly around 6 h and 24 h) and in the chronic phase (7 d and 14 d), with the exception of HSP70 which showed a down-regulation in infected fish. Our results also suggested that lysozyme was evolved in both pro- and anti-inflammatory activities. For genes of the complement system, it was demonstrated that C4 regulation followed the tendency of pro-inflammatory genes. However, the C3 gene was, surprisingly, not expressed in most fish and this corroborated with the results of the complement system activity in serum that also did not show activity in most fish. The possible reasons for the regulation of gene expression and association with fish disease are addressed in this paper. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:48:01Z 2021-06-25T10:48:01Z 2021-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.micpath.2020.104638 Microbial Pathogenesis, v. 150. 1096-1208 0882-4010 http://hdl.handle.net/11449/207038 10.1016/j.micpath.2020.104638 2-s2.0-85098468750 |
url |
http://dx.doi.org/10.1016/j.micpath.2020.104638 http://hdl.handle.net/11449/207038 |
identifier_str_mv |
Microbial Pathogenesis, v. 150. 1096-1208 0882-4010 10.1016/j.micpath.2020.104638 2-s2.0-85098468750 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Microbial Pathogenesis |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965367911579648 |