Dental discoloration caused by Grey-MTAFlow cement: analysis of its physicochemical, biological and antimicrobial properties
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/1678-7757-2020-0269 http://hdl.handle.net/11449/195621 |
Resumo: | Tricalcium silicate-based cement are materials used in reparative and regenerative procedures in endodontics. A recently proposed formulation aimed to enhance handling during clinical use with a versatile material applicable by syringe. Although, the use of bismuth oxide as radiopacifier and grey raw powder are drawbacks considering aesthetics. Objectives: Evaluate physicochemical, biological, and antimicrobial properties of Grey-MTAFlow (Ultradent) and assess whether the addition of zinc oxide (ZnO) prevents dentinal discoloration caused by bismuth oxide. Methodology: Grey-MTAFlow was manipulated in 'thin' consistency for all tests. Luminosity, color change, ion migration to dentine, radiopacity, setting time, ISO 6876:2012 linear flow, volumetric lateral flow and central filling of simulated grooves scanned using micro-computed tomography (pCT), pH, calcium release, volumetric change using pCT, chemical characterisation, cytotoxicity, and antimicrobial activity were assessed. Addition of 5% ZnO to Grey-MTAFlow and a bismuth-containing experimental composition were comparatively tested. Statistical analyses used Shapiro-Wilk, T-test, ANOVA, and Kolmogorov-Smirnov (p<0.05). Results: The addition of ZnO to Grey-MTAFlow prevented dentine darkening after 90 days due to bismuth migration reduction, although no statistical difference was found (p=0.863). ZnO addition significantly enhanced Grey-MTAFlow radiopacity without differences in initial setting time. Grey-MTAFlow presented an ISO linear flow of 10.9 mm and a balanced volumetric lateral flow with central filling in pCT evaluation. All compositions presented an alkaline pH after immersion. Grey-MTAFlow had a significantly higher calcium ion release after 28 days in comparison to 24 hours (p=0.011) and volumetric expansion of 0.4 +/- 1.8% after immersion. ZnO addition altered the hydrated cement matrix once calcium hydroxide (portlandite) could not be detected in characterisation. Neither of the materials produced inhibition halos nor reduced bacterial turbidity, but all presented cytocompatibility above 100%. Conclusion: Grey-MTAFlow expanded after immersion and exhibited higher luminosity values after the evaluation period when ZnO was added, but chemical modifications after this addition occurred. |
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Dental discoloration caused by Grey-MTAFlow cement: analysis of its physicochemical, biological and antimicrobial propertiesTooth discolorationDental materialsBismuthZinc oxideTricalcium silicate-based cement are materials used in reparative and regenerative procedures in endodontics. A recently proposed formulation aimed to enhance handling during clinical use with a versatile material applicable by syringe. Although, the use of bismuth oxide as radiopacifier and grey raw powder are drawbacks considering aesthetics. Objectives: Evaluate physicochemical, biological, and antimicrobial properties of Grey-MTAFlow (Ultradent) and assess whether the addition of zinc oxide (ZnO) prevents dentinal discoloration caused by bismuth oxide. Methodology: Grey-MTAFlow was manipulated in 'thin' consistency for all tests. Luminosity, color change, ion migration to dentine, radiopacity, setting time, ISO 6876:2012 linear flow, volumetric lateral flow and central filling of simulated grooves scanned using micro-computed tomography (pCT), pH, calcium release, volumetric change using pCT, chemical characterisation, cytotoxicity, and antimicrobial activity were assessed. Addition of 5% ZnO to Grey-MTAFlow and a bismuth-containing experimental composition were comparatively tested. Statistical analyses used Shapiro-Wilk, T-test, ANOVA, and Kolmogorov-Smirnov (p<0.05). Results: The addition of ZnO to Grey-MTAFlow prevented dentine darkening after 90 days due to bismuth migration reduction, although no statistical difference was found (p=0.863). ZnO addition significantly enhanced Grey-MTAFlow radiopacity without differences in initial setting time. Grey-MTAFlow presented an ISO linear flow of 10.9 mm and a balanced volumetric lateral flow with central filling in pCT evaluation. All compositions presented an alkaline pH after immersion. Grey-MTAFlow had a significantly higher calcium ion release after 28 days in comparison to 24 hours (p=0.011) and volumetric expansion of 0.4 +/- 1.8% after immersion. ZnO addition altered the hydrated cement matrix once calcium hydroxide (portlandite) could not be detected in characterisation. Neither of the materials produced inhibition halos nor reduced bacterial turbidity, but all presented cytocompatibility above 100%. Conclusion: Grey-MTAFlow expanded after immersion and exhibited higher luminosity values after the evaluation period when ZnO was added, but chemical modifications after this addition occurred.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Campinas, Fac Odontol Piracicaba, Sao Paulo, BrazilUniv Sao Paulo, Fac Odontol Bauru, Sao Paulo, BrazilUniv Estadual Paulista, Fac Odontol Araraquara, UNESP, Araraquara, SP, BrazilUniv Estadual Paulista, Fac Odontol Araraquara, UNESP, Araraquara, SP, BrazilFAPESP: 2017/05096-7FAPESP: 2017/17785-1FAPESP: 2018/065156FAPESP: 2018/06903-6FAPESP: 2018/166722-9Univ Sao Paulo Fac Odontologia BauruUniversidade Estadual de Campinas (UNICAMP)Universidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Pelepenko, Lauter EstonSaavedra, FlaviaBombarda, Gabriela FernandaFigueiredo de Almeida Gomes, Brenda Paulade-Jesus-Soares, AdrianaZaia, Alexandre AugustoHungaro Duarte, Marco AntonioTanomaru-Filho, Mario [UNESP]Marciano, Marina Angelica2020-12-10T17:40:48Z2020-12-10T17:40:48Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article15application/pdfhttp://dx.doi.org/10.1590/1678-7757-2020-0269Journal Of Applied Oral Science. Bauru-sp: Univ Sao Paulo Fac Odontologia Bauru, v. 28, 15 p., 2020.1678-7757http://hdl.handle.net/11449/19562110.1590/1678-7757-2020-0269S1678-77572020000100462WOS:000562150700001S1678-77572020000100462.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal Of Applied Oral Scienceinfo:eu-repo/semantics/openAccess2023-12-23T06:17:26Zoai:repositorio.unesp.br:11449/195621Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-23T06:17:26Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Dental discoloration caused by Grey-MTAFlow cement: analysis of its physicochemical, biological and antimicrobial properties |
title |
Dental discoloration caused by Grey-MTAFlow cement: analysis of its physicochemical, biological and antimicrobial properties |
spellingShingle |
Dental discoloration caused by Grey-MTAFlow cement: analysis of its physicochemical, biological and antimicrobial properties Pelepenko, Lauter Eston Tooth discoloration Dental materials Bismuth Zinc oxide |
title_short |
Dental discoloration caused by Grey-MTAFlow cement: analysis of its physicochemical, biological and antimicrobial properties |
title_full |
Dental discoloration caused by Grey-MTAFlow cement: analysis of its physicochemical, biological and antimicrobial properties |
title_fullStr |
Dental discoloration caused by Grey-MTAFlow cement: analysis of its physicochemical, biological and antimicrobial properties |
title_full_unstemmed |
Dental discoloration caused by Grey-MTAFlow cement: analysis of its physicochemical, biological and antimicrobial properties |
title_sort |
Dental discoloration caused by Grey-MTAFlow cement: analysis of its physicochemical, biological and antimicrobial properties |
author |
Pelepenko, Lauter Eston |
author_facet |
Pelepenko, Lauter Eston Saavedra, Flavia Bombarda, Gabriela Fernanda Figueiredo de Almeida Gomes, Brenda Paula de-Jesus-Soares, Adriana Zaia, Alexandre Augusto Hungaro Duarte, Marco Antonio Tanomaru-Filho, Mario [UNESP] Marciano, Marina Angelica |
author_role |
author |
author2 |
Saavedra, Flavia Bombarda, Gabriela Fernanda Figueiredo de Almeida Gomes, Brenda Paula de-Jesus-Soares, Adriana Zaia, Alexandre Augusto Hungaro Duarte, Marco Antonio Tanomaru-Filho, Mario [UNESP] Marciano, Marina Angelica |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Pelepenko, Lauter Eston Saavedra, Flavia Bombarda, Gabriela Fernanda Figueiredo de Almeida Gomes, Brenda Paula de-Jesus-Soares, Adriana Zaia, Alexandre Augusto Hungaro Duarte, Marco Antonio Tanomaru-Filho, Mario [UNESP] Marciano, Marina Angelica |
dc.subject.por.fl_str_mv |
Tooth discoloration Dental materials Bismuth Zinc oxide |
topic |
Tooth discoloration Dental materials Bismuth Zinc oxide |
description |
Tricalcium silicate-based cement are materials used in reparative and regenerative procedures in endodontics. A recently proposed formulation aimed to enhance handling during clinical use with a versatile material applicable by syringe. Although, the use of bismuth oxide as radiopacifier and grey raw powder are drawbacks considering aesthetics. Objectives: Evaluate physicochemical, biological, and antimicrobial properties of Grey-MTAFlow (Ultradent) and assess whether the addition of zinc oxide (ZnO) prevents dentinal discoloration caused by bismuth oxide. Methodology: Grey-MTAFlow was manipulated in 'thin' consistency for all tests. Luminosity, color change, ion migration to dentine, radiopacity, setting time, ISO 6876:2012 linear flow, volumetric lateral flow and central filling of simulated grooves scanned using micro-computed tomography (pCT), pH, calcium release, volumetric change using pCT, chemical characterisation, cytotoxicity, and antimicrobial activity were assessed. Addition of 5% ZnO to Grey-MTAFlow and a bismuth-containing experimental composition were comparatively tested. Statistical analyses used Shapiro-Wilk, T-test, ANOVA, and Kolmogorov-Smirnov (p<0.05). Results: The addition of ZnO to Grey-MTAFlow prevented dentine darkening after 90 days due to bismuth migration reduction, although no statistical difference was found (p=0.863). ZnO addition significantly enhanced Grey-MTAFlow radiopacity without differences in initial setting time. Grey-MTAFlow presented an ISO linear flow of 10.9 mm and a balanced volumetric lateral flow with central filling in pCT evaluation. All compositions presented an alkaline pH after immersion. Grey-MTAFlow had a significantly higher calcium ion release after 28 days in comparison to 24 hours (p=0.011) and volumetric expansion of 0.4 +/- 1.8% after immersion. ZnO addition altered the hydrated cement matrix once calcium hydroxide (portlandite) could not be detected in characterisation. Neither of the materials produced inhibition halos nor reduced bacterial turbidity, but all presented cytocompatibility above 100%. Conclusion: Grey-MTAFlow expanded after immersion and exhibited higher luminosity values after the evaluation period when ZnO was added, but chemical modifications after this addition occurred. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-10T17:40:48Z 2020-12-10T17:40:48Z 2020-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1678-7757-2020-0269 Journal Of Applied Oral Science. Bauru-sp: Univ Sao Paulo Fac Odontologia Bauru, v. 28, 15 p., 2020. 1678-7757 http://hdl.handle.net/11449/195621 10.1590/1678-7757-2020-0269 S1678-77572020000100462 WOS:000562150700001 S1678-77572020000100462.pdf |
url |
http://dx.doi.org/10.1590/1678-7757-2020-0269 http://hdl.handle.net/11449/195621 |
identifier_str_mv |
Journal Of Applied Oral Science. Bauru-sp: Univ Sao Paulo Fac Odontologia Bauru, v. 28, 15 p., 2020. 1678-7757 10.1590/1678-7757-2020-0269 S1678-77572020000100462 WOS:000562150700001 S1678-77572020000100462.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal Of Applied Oral Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
15 application/pdf |
dc.publisher.none.fl_str_mv |
Univ Sao Paulo Fac Odontologia Bauru |
publisher.none.fl_str_mv |
Univ Sao Paulo Fac Odontologia Bauru |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1797790084289789952 |