Hematological, biochemical, and histopathological responses in sorubim Pseudoplatystoma spp. experimentally infected with Lactococcus garvieae
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s10499-020-00566-5 http://hdl.handle.net/11449/200691 |
Resumo: | This study evaluated the hematological, biochemical, and histopathological responses of sorubim Pseudoplatystoma spp., experimentally infected with Lactococcus garvieae. Sorubim juveniles (n = 66; 21.3 ± 5.5 g; 17.1 ± 1.3 cm) were infected with L. garvieae by coelmatic inoculation (1.5 × 108 CFU mL−1) and sampling to 1, 3, 7, 14, and 21 days post-inoculation (dpi) for evaluation of hematological, biochemical parameters. Histopathological data of the spleen and kidney were estimated according to the histological alteration index (HAIn). Additionally, a decrease of hematocrit (1, 3, and 14 dpi), hemoglobin, and count of erythrocytes (3, 14, and 21 dpi), blood glucose (3 dpi), and proteinemy (14 dpi) were observed. Monocytes decreased at 7, 14, and 21 dpi; neutrophils at 3, 7, and 21 dpi; and lymphocytes at 21 dpi. Edema, hyperemia, hemorrhage, inflammatory infiltrate, and necrosis were the major histological changes observed in the spleen and kidney. HAIn was higher at 7 to 14 dpi in the spleen. Edema, hyperemia, and hyperplasia with hypertrophy of melanomacrophage centers (MMCs) associated with mild focal necrosis areas, particularly in white pulp, were observed. In the head kidney, it was observed that MMCs and melanomacrophages surrounded the capillaries and dispersed inside the sinusoidal capillaries network. Internalized bacteria by macrophages were commonly observed at 3 to 7 dpi. Tubular neogenesis characterized by nephrogenic bodies and rare MMCs at 21 dpi was observed. The experimental infection with L. garvieae caused significant hematological and biochemical responses. Pathological manifestations are closely related to the spleen and head kidneys mainly represented by hyperplasia and hypertrophy of melanomacrophage centers and mild necrosis areas. |
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Hematological, biochemical, and histopathological responses in sorubim Pseudoplatystoma spp. experimentally infected with Lactococcus garvieaeHematologyHistopathologyKidneyLactococcosisSpleenThis study evaluated the hematological, biochemical, and histopathological responses of sorubim Pseudoplatystoma spp., experimentally infected with Lactococcus garvieae. Sorubim juveniles (n = 66; 21.3 ± 5.5 g; 17.1 ± 1.3 cm) were infected with L. garvieae by coelmatic inoculation (1.5 × 108 CFU mL−1) and sampling to 1, 3, 7, 14, and 21 days post-inoculation (dpi) for evaluation of hematological, biochemical parameters. Histopathological data of the spleen and kidney were estimated according to the histological alteration index (HAIn). Additionally, a decrease of hematocrit (1, 3, and 14 dpi), hemoglobin, and count of erythrocytes (3, 14, and 21 dpi), blood glucose (3 dpi), and proteinemy (14 dpi) were observed. Monocytes decreased at 7, 14, and 21 dpi; neutrophils at 3, 7, and 21 dpi; and lymphocytes at 21 dpi. Edema, hyperemia, hemorrhage, inflammatory infiltrate, and necrosis were the major histological changes observed in the spleen and kidney. HAIn was higher at 7 to 14 dpi in the spleen. Edema, hyperemia, and hyperplasia with hypertrophy of melanomacrophage centers (MMCs) associated with mild focal necrosis areas, particularly in white pulp, were observed. In the head kidney, it was observed that MMCs and melanomacrophages surrounded the capillaries and dispersed inside the sinusoidal capillaries network. Internalized bacteria by macrophages were commonly observed at 3 to 7 dpi. Tubular neogenesis characterized by nephrogenic bodies and rare MMCs at 21 dpi was observed. The experimental infection with L. garvieae caused significant hematological and biochemical responses. Pathological manifestations are closely related to the spleen and head kidneys mainly represented by hyperplasia and hypertrophy of melanomacrophage centers and mild necrosis areas.Fundação de Apoio ao Desenvolvimento do Ensino, Ciência e Tecnologia do Estado de Mato Grosso do SulDepartamento de Aquicultura Universidade Federal de Santa Catarina-UFSCLaboratório de Patologia Experimental LAPEx Instituto de Biociências – INBIO Universidade Federal de Mato Grosso do Sul – UFMSPrograma de Pós-Graduação em Ciência Animal Faculdade de Medicina Veterinária e Zootecnia – FAMEZ Universidade Federal de Mato Grosso do Sul – UFMSLaboratório de Microbiologia e Parasitologia de Organismos Aquáticos Centro de Aquicultura da UNESPPrograma de Pós-Graduação em Ciências Veterinárias Faculdade de Medicina Veterinária e Zootecnia – FAMEZ Universidade Federal de Mato Grosso do Sul – UFMSDiagno Vet Laboratório VeterinárioDiagno Vet Laboratório Veterinário Universidade Estadual de Mato Grosso do Sul – UEMS Unidade Universitária de AquidauanaLaboratório de Microbiologia e Parasitologia de Organismos Aquáticos Centro de Aquicultura da UNESPFundação de Apoio ao Desenvolvimento do Ensino, Ciência e Tecnologia do Estado de Mato Grosso do Sul: 23/200.233/2014Universidade Federal de Santa Catarina (UFSC)Universidade Federal de Mato Grosso do Sul (UFMS)Universidade Estadual Paulista (Unesp)Diagno Vet Laboratório VeterinárioUniversidade Estadual de Mato Grosso do Sul (UEMS)Rodrigues, Robson Andradedo Nascimento Silva, André LuizSiqueira, Mayara SchueroffPilarski, Fabiana [UNESP]Leal, Cassia Rejane BritoKuibida, Karin Virgíniade Campos, Cristiane MeldauFernandes, Carlos Eurico2020-12-12T02:13:29Z2020-12-12T02:13:29Z2020-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1907-1923http://dx.doi.org/10.1007/s10499-020-00566-5Aquaculture International, v. 28, n. 5, p. 1907-1923, 2020.1573-143X0967-6120http://hdl.handle.net/11449/20069110.1007/s10499-020-00566-52-s2.0-85087360457Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAquaculture Internationalinfo:eu-repo/semantics/openAccess2024-04-09T15:43:16Zoai:repositorio.unesp.br:11449/200691Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-04-09T15:43:16Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Hematological, biochemical, and histopathological responses in sorubim Pseudoplatystoma spp. experimentally infected with Lactococcus garvieae |
title |
Hematological, biochemical, and histopathological responses in sorubim Pseudoplatystoma spp. experimentally infected with Lactococcus garvieae |
spellingShingle |
Hematological, biochemical, and histopathological responses in sorubim Pseudoplatystoma spp. experimentally infected with Lactococcus garvieae Rodrigues, Robson Andrade Hematology Histopathology Kidney Lactococcosis Spleen |
title_short |
Hematological, biochemical, and histopathological responses in sorubim Pseudoplatystoma spp. experimentally infected with Lactococcus garvieae |
title_full |
Hematological, biochemical, and histopathological responses in sorubim Pseudoplatystoma spp. experimentally infected with Lactococcus garvieae |
title_fullStr |
Hematological, biochemical, and histopathological responses in sorubim Pseudoplatystoma spp. experimentally infected with Lactococcus garvieae |
title_full_unstemmed |
Hematological, biochemical, and histopathological responses in sorubim Pseudoplatystoma spp. experimentally infected with Lactococcus garvieae |
title_sort |
Hematological, biochemical, and histopathological responses in sorubim Pseudoplatystoma spp. experimentally infected with Lactococcus garvieae |
author |
Rodrigues, Robson Andrade |
author_facet |
Rodrigues, Robson Andrade do Nascimento Silva, André Luiz Siqueira, Mayara Schueroff Pilarski, Fabiana [UNESP] Leal, Cassia Rejane Brito Kuibida, Karin Virgínia de Campos, Cristiane Meldau Fernandes, Carlos Eurico |
author_role |
author |
author2 |
do Nascimento Silva, André Luiz Siqueira, Mayara Schueroff Pilarski, Fabiana [UNESP] Leal, Cassia Rejane Brito Kuibida, Karin Virgínia de Campos, Cristiane Meldau Fernandes, Carlos Eurico |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Santa Catarina (UFSC) Universidade Federal de Mato Grosso do Sul (UFMS) Universidade Estadual Paulista (Unesp) Diagno Vet Laboratório Veterinário Universidade Estadual de Mato Grosso do Sul (UEMS) |
dc.contributor.author.fl_str_mv |
Rodrigues, Robson Andrade do Nascimento Silva, André Luiz Siqueira, Mayara Schueroff Pilarski, Fabiana [UNESP] Leal, Cassia Rejane Brito Kuibida, Karin Virgínia de Campos, Cristiane Meldau Fernandes, Carlos Eurico |
dc.subject.por.fl_str_mv |
Hematology Histopathology Kidney Lactococcosis Spleen |
topic |
Hematology Histopathology Kidney Lactococcosis Spleen |
description |
This study evaluated the hematological, biochemical, and histopathological responses of sorubim Pseudoplatystoma spp., experimentally infected with Lactococcus garvieae. Sorubim juveniles (n = 66; 21.3 ± 5.5 g; 17.1 ± 1.3 cm) were infected with L. garvieae by coelmatic inoculation (1.5 × 108 CFU mL−1) and sampling to 1, 3, 7, 14, and 21 days post-inoculation (dpi) for evaluation of hematological, biochemical parameters. Histopathological data of the spleen and kidney were estimated according to the histological alteration index (HAIn). Additionally, a decrease of hematocrit (1, 3, and 14 dpi), hemoglobin, and count of erythrocytes (3, 14, and 21 dpi), blood glucose (3 dpi), and proteinemy (14 dpi) were observed. Monocytes decreased at 7, 14, and 21 dpi; neutrophils at 3, 7, and 21 dpi; and lymphocytes at 21 dpi. Edema, hyperemia, hemorrhage, inflammatory infiltrate, and necrosis were the major histological changes observed in the spleen and kidney. HAIn was higher at 7 to 14 dpi in the spleen. Edema, hyperemia, and hyperplasia with hypertrophy of melanomacrophage centers (MMCs) associated with mild focal necrosis areas, particularly in white pulp, were observed. In the head kidney, it was observed that MMCs and melanomacrophages surrounded the capillaries and dispersed inside the sinusoidal capillaries network. Internalized bacteria by macrophages were commonly observed at 3 to 7 dpi. Tubular neogenesis characterized by nephrogenic bodies and rare MMCs at 21 dpi was observed. The experimental infection with L. garvieae caused significant hematological and biochemical responses. Pathological manifestations are closely related to the spleen and head kidneys mainly represented by hyperplasia and hypertrophy of melanomacrophage centers and mild necrosis areas. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:13:29Z 2020-12-12T02:13:29Z 2020-10-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s10499-020-00566-5 Aquaculture International, v. 28, n. 5, p. 1907-1923, 2020. 1573-143X 0967-6120 http://hdl.handle.net/11449/200691 10.1007/s10499-020-00566-5 2-s2.0-85087360457 |
url |
http://dx.doi.org/10.1007/s10499-020-00566-5 http://hdl.handle.net/11449/200691 |
identifier_str_mv |
Aquaculture International, v. 28, n. 5, p. 1907-1923, 2020. 1573-143X 0967-6120 10.1007/s10499-020-00566-5 2-s2.0-85087360457 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Aquaculture International |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1907-1923 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1797790161367465984 |