Decarboxylation mechanisms of C4 photosynthesis in Saccharum spp.: Increased PEPCK activity under water-limiting conditions
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/s12870-019-1745-7 http://hdl.handle.net/11449/188985 |
Resumo: | Background: C4 plants have been classified into three subtypes based on the enzymes used to decarboxylate C4 acids in the bundle sheath cells (NADP-ME, NAD-ME and PEPCK pathways). Evidences indicate that, depending on environmental factors, C4 plants may exhibit a certain degree of flexibility in the use of the decarboxylation mechanisms. In this context, the objective was to extend the knowledge on the degree of flexibility between the pathways of decarboxylation in sugarcane, a NADP-ME species, at different levels of water deficit. Results: An experiment was carried out with two cultivars - RB92579 (tolerant to water deficit) and SP80-3280 (susceptible to water deficit) subjected to moderate level (- 1.5 to - 1.8 MPa), severe level (below - 2.0 MPa) and recovery (48 h after rehydration) and changes in the activities of the enzymes involved in the three C4 mechanisms and in gene expression were investigated. Our results showed that sugarcane uses the PEPCK pathway as a decarboxylation mechanism in addition to the NADP-ME, which was more evident under water deficit conditions for both cultivars. Conclusions: The results obtained here, show that sugarcane increases the use of the PEPCK pathway as a decarboxylation mechanism, in addition to the NADP-ME pathway, under conditions of water deficit, particularly in the tolerant cultivar. |
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spelling |
Decarboxylation mechanisms of C4 photosynthesis in Saccharum spp.: Increased PEPCK activity under water-limiting conditionsSaccharum spp. drought stress. C4 photosynthesis. Decarboxylation mechanisms. Gene regulationBackground: C4 plants have been classified into three subtypes based on the enzymes used to decarboxylate C4 acids in the bundle sheath cells (NADP-ME, NAD-ME and PEPCK pathways). Evidences indicate that, depending on environmental factors, C4 plants may exhibit a certain degree of flexibility in the use of the decarboxylation mechanisms. In this context, the objective was to extend the knowledge on the degree of flexibility between the pathways of decarboxylation in sugarcane, a NADP-ME species, at different levels of water deficit. Results: An experiment was carried out with two cultivars - RB92579 (tolerant to water deficit) and SP80-3280 (susceptible to water deficit) subjected to moderate level (- 1.5 to - 1.8 MPa), severe level (below - 2.0 MPa) and recovery (48 h after rehydration) and changes in the activities of the enzymes involved in the three C4 mechanisms and in gene expression were investigated. Our results showed that sugarcane uses the PEPCK pathway as a decarboxylation mechanism in addition to the NADP-ME, which was more evident under water deficit conditions for both cultivars. Conclusions: The results obtained here, show that sugarcane increases the use of the PEPCK pathway as a decarboxylation mechanism, in addition to the NADP-ME pathway, under conditions of water deficit, particularly in the tolerant cultivar.Centro de Estudos em Ecofisiologia Vegetal Do Oeste Paulista (CEVOP) Universidade Do Oeste Paulista (UNOESTE), Rodovia Raposo Tavares, Km 572, CEPAgronomy Graduate Program Universidade Do Oeste Paulista (UNOESTE), Rodovia Raposo Tavares, Km 572, CEPDepartamento de Botânica Instituto de Biociências de Rio Claro Universidade Estadual Paulista (UNESP), Avenida 24-A, 1515, CEPDepartamento de Botânica Instituto de Biociências de Rio Claro Universidade Estadual Paulista (UNESP), Avenida 24-A, 1515, CEPUniversidade Do Oeste Paulista (UNOESTE)Universidade Estadual Paulista (Unesp)Cacefo, VivianeRibas, Alessandra FerreiraZilliani, Rafael RebesNeris, Daniel MoreiraDomingues, Douglas Silva [UNESP]Moro, Adriana LimaVieira, Luiz Gonzaga Esteves2019-10-06T16:26:01Z2019-10-06T16:26:01Z2019-04-16info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1186/s12870-019-1745-7BMC Plant Biology, v. 19, n. 1, 2019.1471-2229http://hdl.handle.net/11449/18898510.1186/s12870-019-1745-72-s2.0-85064404562Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBMC Plant Biologyinfo:eu-repo/semantics/openAccess2021-10-22T21:16:11Zoai:repositorio.unesp.br:11449/188985Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-22T21:16:11Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Decarboxylation mechanisms of C4 photosynthesis in Saccharum spp.: Increased PEPCK activity under water-limiting conditions |
title |
Decarboxylation mechanisms of C4 photosynthesis in Saccharum spp.: Increased PEPCK activity under water-limiting conditions |
spellingShingle |
Decarboxylation mechanisms of C4 photosynthesis in Saccharum spp.: Increased PEPCK activity under water-limiting conditions Cacefo, Viviane Saccharum spp. drought stress. C4 photosynthesis. Decarboxylation mechanisms. Gene regulation |
title_short |
Decarboxylation mechanisms of C4 photosynthesis in Saccharum spp.: Increased PEPCK activity under water-limiting conditions |
title_full |
Decarboxylation mechanisms of C4 photosynthesis in Saccharum spp.: Increased PEPCK activity under water-limiting conditions |
title_fullStr |
Decarboxylation mechanisms of C4 photosynthesis in Saccharum spp.: Increased PEPCK activity under water-limiting conditions |
title_full_unstemmed |
Decarboxylation mechanisms of C4 photosynthesis in Saccharum spp.: Increased PEPCK activity under water-limiting conditions |
title_sort |
Decarboxylation mechanisms of C4 photosynthesis in Saccharum spp.: Increased PEPCK activity under water-limiting conditions |
author |
Cacefo, Viviane |
author_facet |
Cacefo, Viviane Ribas, Alessandra Ferreira Zilliani, Rafael Rebes Neris, Daniel Moreira Domingues, Douglas Silva [UNESP] Moro, Adriana Lima Vieira, Luiz Gonzaga Esteves |
author_role |
author |
author2 |
Ribas, Alessandra Ferreira Zilliani, Rafael Rebes Neris, Daniel Moreira Domingues, Douglas Silva [UNESP] Moro, Adriana Lima Vieira, Luiz Gonzaga Esteves |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Do Oeste Paulista (UNOESTE) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Cacefo, Viviane Ribas, Alessandra Ferreira Zilliani, Rafael Rebes Neris, Daniel Moreira Domingues, Douglas Silva [UNESP] Moro, Adriana Lima Vieira, Luiz Gonzaga Esteves |
dc.subject.por.fl_str_mv |
Saccharum spp. drought stress. C4 photosynthesis. Decarboxylation mechanisms. Gene regulation |
topic |
Saccharum spp. drought stress. C4 photosynthesis. Decarboxylation mechanisms. Gene regulation |
description |
Background: C4 plants have been classified into three subtypes based on the enzymes used to decarboxylate C4 acids in the bundle sheath cells (NADP-ME, NAD-ME and PEPCK pathways). Evidences indicate that, depending on environmental factors, C4 plants may exhibit a certain degree of flexibility in the use of the decarboxylation mechanisms. In this context, the objective was to extend the knowledge on the degree of flexibility between the pathways of decarboxylation in sugarcane, a NADP-ME species, at different levels of water deficit. Results: An experiment was carried out with two cultivars - RB92579 (tolerant to water deficit) and SP80-3280 (susceptible to water deficit) subjected to moderate level (- 1.5 to - 1.8 MPa), severe level (below - 2.0 MPa) and recovery (48 h after rehydration) and changes in the activities of the enzymes involved in the three C4 mechanisms and in gene expression were investigated. Our results showed that sugarcane uses the PEPCK pathway as a decarboxylation mechanism in addition to the NADP-ME, which was more evident under water deficit conditions for both cultivars. Conclusions: The results obtained here, show that sugarcane increases the use of the PEPCK pathway as a decarboxylation mechanism, in addition to the NADP-ME pathway, under conditions of water deficit, particularly in the tolerant cultivar. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T16:26:01Z 2019-10-06T16:26:01Z 2019-04-16 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/s12870-019-1745-7 BMC Plant Biology, v. 19, n. 1, 2019. 1471-2229 http://hdl.handle.net/11449/188985 10.1186/s12870-019-1745-7 2-s2.0-85064404562 |
url |
http://dx.doi.org/10.1186/s12870-019-1745-7 http://hdl.handle.net/11449/188985 |
identifier_str_mv |
BMC Plant Biology, v. 19, n. 1, 2019. 1471-2229 10.1186/s12870-019-1745-7 2-s2.0-85064404562 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
BMC Plant Biology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797790284447219712 |