Acute exposure to hyperosmotic conditions reduces sperm activation by urine in the yellowtail tetra astyanax altiparanae, a freshwater teleost fish

• Autor(a) principal: Rocha, Nathalia Alcântara [UNESP]
• Data de Publicação: 2020
• Outros Autores: Schade, Gabriel Marra [UNESP], Alves, Álvaro de Miranda, Silva, Claudia de Souza, Nakirimoto, Jacqueline Megumi, Lauri, Liura Sanchez, Campos, Lucca Gobatto, Santos, Marcelo Galvão Dos, Mesquita, Nicole Nascimento, Relvas, Rachel Sordi, Carnevale, Rafaella Fernandes, Oh, Se Yoon, Gaia, Victoria Portela Diniz, Kida, Vivian Renata, Celeghini, Eneiva Carla Carvalho, Assumpção, Mayra Elena Ortiz D’Avila, Senhorini, José Augusto [UNESP], Siqueira, Hatus de Oliveira [UNESP], Nascimento, Nivaldo Ferreira [UNESP], Silva, Luciano Andrade, Lázaro, Talita Maria, Visintin, José Antonio, Monzani, Paulo Sérgio, Yasui, George Shigueki [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.11606/issn.1678-4456.bjvras.2020.166205; http://hdl.handle.net/11449/228861
• topic: Fish; Salinity; Sperm contamination; Urospermia

In freshwater fish with external fertilization, sperm sampling can be contaminated with urine, which triggers motility and gives rise to decreased fertilization success. The maintenance of freshwater fish in hyperosmotic conditions may reduce urine production and improve sperm quality. Thus, the aim of this work was to verify if acute exposure to various NaCl concentrations improves sperm quality in the yellowtail tetra Astyanax altiparanae. Spermiation was induced using a single dose of carp pituitary gland (5 mg kg-1) and the males were maintained at various NaCl concentrations: NaCl 0.00% (control), NaCl 0.45% (hypoosmotic), NaCl 0.9% (isosmotic) and NaCl 1.0% (hyperosmotic) for 6 h at 26 °C. Sperm was collected and verified for activation by urine and motility traits. At 0.00%, 0.45%, and 0.90%, the sperm was motile just after sampling, indicating activation by urine. Surprisingly, at hyperosmotic conditions, no activation was observed. Other sperm and motility parameters did not show any statistical differences, including sperm viability (P = 0.7083), concentration (P = 0.9030), total motility (P = 0.6149), VCL (curvilinear velocity; P = 0.1216), VAP (average path velocity; P = 0.1231) and VSL (straight-line velocity; P = 0.1340). Our results indicate that acute maintenance at hyperosmotic conditions eliminates sperm activation by urine and maintains sperm quality. Such a new procedure is interesting for both basic and applied sciences, including reproductive practice in fish.