Influência do ciclo estral na maturação nuclear de oócitos caninos

Detalhes bibliográficos
Autor(a) principal: Pereira, L. M.C. [UNESP]
Data de Publicação: 2013
Outros Autores: Bersano, P. R.O. [UNESP], Lopes, M. D. [UNESP]
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://hdl.handle.net/11449/227495
Resumo: The aim of this study is to evaluate the relationship between the phases of diestrus and anestrus with nuclear maturation of canine oocytes. A total of 36 female dogs, subject to elective ovariohysterectomy in Clinic of Small Animal Reproduction FMVZ / UNESP, Botucatu. Ovaries were sliced and selected cumulus-oocyte complexes (COCs) degree 1. COCs were cultured in maturation medium for 72 h, subjected to hyaluronidase solution 0,2% and stained with Hoechst. The oocytes were evaluated by light microscopy and fluorescence. The Fisher exact test was used to estimate the association between the estrous cycle and nuclear maturation. The results showed no significant difference (p>0,05) between rates of maturation of oocytes of different stages.
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spelling Influência do ciclo estral na maturação nuclear de oócitos caninosInfluence of estrous cycle in nuclear maturation of canine oocytesBitchesEstrous cycleNuclear maturationOocytesThe aim of this study is to evaluate the relationship between the phases of diestrus and anestrus with nuclear maturation of canine oocytes. A total of 36 female dogs, subject to elective ovariohysterectomy in Clinic of Small Animal Reproduction FMVZ / UNESP, Botucatu. Ovaries were sliced and selected cumulus-oocyte complexes (COCs) degree 1. COCs were cultured in maturation medium for 72 h, subjected to hyaluronidase solution 0,2% and stained with Hoechst. The oocytes were evaluated by light microscopy and fluorescence. The Fisher exact test was used to estimate the association between the estrous cycle and nuclear maturation. The results showed no significant difference (p>0,05) between rates of maturation of oocytes of different stages.UNESP, BotucatuDepartamento de Clínica Veterinária UNESP, BotucatuDepartamento de Reprodução Animal e Radiologia Veterinária UNESP, BotucatuUNESP, BotucatuDepartamento de Clínica Veterinária UNESP, BotucatuDepartamento de Reprodução Animal e Radiologia Veterinária UNESP, BotucatuUniversidade Estadual Paulista (UNESP)Pereira, L. M.C. [UNESP]Bersano, P. R.O. [UNESP]Lopes, M. D. [UNESP]2022-04-29T07:13:29Z2022-04-29T07:13:29Z2013-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article354-356Acta Veterinaria Brasilica, v. 7, n. SUPPL. 1, p. 354-356, 2013.1981-5484http://hdl.handle.net/11449/2274952-s2.0-84892773947Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporActa Veterinaria Brasilicainfo:eu-repo/semantics/openAccess2022-04-29T07:13:29Zoai:repositorio.unesp.br:11449/227495Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-29T07:13:29Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Influência do ciclo estral na maturação nuclear de oócitos caninos
Influence of estrous cycle in nuclear maturation of canine oocytes
title Influência do ciclo estral na maturação nuclear de oócitos caninos
spellingShingle Influência do ciclo estral na maturação nuclear de oócitos caninos
Pereira, L. M.C. [UNESP]
Bitches
Estrous cycle
Nuclear maturation
Oocytes
title_short Influência do ciclo estral na maturação nuclear de oócitos caninos
title_full Influência do ciclo estral na maturação nuclear de oócitos caninos
title_fullStr Influência do ciclo estral na maturação nuclear de oócitos caninos
title_full_unstemmed Influência do ciclo estral na maturação nuclear de oócitos caninos
title_sort Influência do ciclo estral na maturação nuclear de oócitos caninos
author Pereira, L. M.C. [UNESP]
author_facet Pereira, L. M.C. [UNESP]
Bersano, P. R.O. [UNESP]
Lopes, M. D. [UNESP]
author_role author
author2 Bersano, P. R.O. [UNESP]
Lopes, M. D. [UNESP]
author2_role author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Pereira, L. M.C. [UNESP]
Bersano, P. R.O. [UNESP]
Lopes, M. D. [UNESP]
dc.subject.por.fl_str_mv Bitches
Estrous cycle
Nuclear maturation
Oocytes
topic Bitches
Estrous cycle
Nuclear maturation
Oocytes
description The aim of this study is to evaluate the relationship between the phases of diestrus and anestrus with nuclear maturation of canine oocytes. A total of 36 female dogs, subject to elective ovariohysterectomy in Clinic of Small Animal Reproduction FMVZ / UNESP, Botucatu. Ovaries were sliced and selected cumulus-oocyte complexes (COCs) degree 1. COCs were cultured in maturation medium for 72 h, subjected to hyaluronidase solution 0,2% and stained with Hoechst. The oocytes were evaluated by light microscopy and fluorescence. The Fisher exact test was used to estimate the association between the estrous cycle and nuclear maturation. The results showed no significant difference (p>0,05) between rates of maturation of oocytes of different stages.
publishDate 2013
dc.date.none.fl_str_mv 2013-12-01
2022-04-29T07:13:29Z
2022-04-29T07:13:29Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Acta Veterinaria Brasilica, v. 7, n. SUPPL. 1, p. 354-356, 2013.
1981-5484
http://hdl.handle.net/11449/227495
2-s2.0-84892773947
identifier_str_mv Acta Veterinaria Brasilica, v. 7, n. SUPPL. 1, p. 354-356, 2013.
1981-5484
2-s2.0-84892773947
url http://hdl.handle.net/11449/227495
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv Acta Veterinaria Brasilica
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 354-356
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1792961880950571008

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