Applying CeO2 nanorods in flexible electrochemical immunosensor to detect C-reactive protein
Autor(a) principal: | |
---|---|
Data de Publicação: | 2023 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.jelechem.2023.117353 http://hdl.handle.net/11449/247039 |
Resumo: | In this work, we report the development of a flexible electrochemical immunosensor based on CeO2 nanorods (NRs) immobilized with C-reactive protein (CRP) antibody, a biomarker of cardiovascular disease. CeO2 NRs were obtained by microwave-assisted hydrothermal method and characterized by XRD, TEM, and XPS. For the preparation of the working electrode, WE, of the immunosensor, the NRs were dispersed in organic solvent and then functionalized with cystamine and glutaraldehyde, and then modified with CRP antibody. The deposition condition was evaluated for electrode functionalization and immobilization. Functionalization and immobilization were verified by spectroscopic techniques and proved effective repeatability and reproducibility. The developed immunosensor had acceptable reproducibility (coefficient of variation = 4.5 %), allowed detection in the range of 0.3 to 7.0 mg L−1 of CRP with a detection limit of 0.18 mg L−1, and applications that are immediately detectable, easy-to-handle, low-cost and ideal for point-of-care. |
id |
UNSP_5b990bcee9e0d6cf6a96d02a67d5a925 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/247039 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Applying CeO2 nanorods in flexible electrochemical immunosensor to detect C-reactive proteinCeO2CRPImmunosensorNanoparticlesIn this work, we report the development of a flexible electrochemical immunosensor based on CeO2 nanorods (NRs) immobilized with C-reactive protein (CRP) antibody, a biomarker of cardiovascular disease. CeO2 NRs were obtained by microwave-assisted hydrothermal method and characterized by XRD, TEM, and XPS. For the preparation of the working electrode, WE, of the immunosensor, the NRs were dispersed in organic solvent and then functionalized with cystamine and glutaraldehyde, and then modified with CRP antibody. The deposition condition was evaluated for electrode functionalization and immobilization. Functionalization and immobilization were verified by spectroscopic techniques and proved effective repeatability and reproducibility. The developed immunosensor had acceptable reproducibility (coefficient of variation = 4.5 %), allowed detection in the range of 0.3 to 7.0 mg L−1 of CRP with a detection limit of 0.18 mg L−1, and applications that are immediately detectable, easy-to-handle, low-cost and ideal for point-of-care.School of Engineering São Paulo State University – UNESP, São PauloCenter for Engineering Modeling and Applied Social Sciences Federal University of ABC, Dos Estados Avenue, 5001, SPCenter for Information Technology Renato Archer CTI, Rod. D. Pedro I, KM 143.6, SPSchool of Engineering São Paulo State University – UNESP, São PauloUniversidade Estadual Paulista (UNESP)Federal University of ABCCTIAmoresi, Rafael Aparecido Ciola [UNESP]Roza, Noemí Angélica VieiraMazon, Talita2023-07-29T12:57:25Z2023-07-29T12:57:25Z2023-04-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.jelechem.2023.117353Journal of Electroanalytical Chemistry, v. 935.1572-6657http://hdl.handle.net/11449/24703910.1016/j.jelechem.2023.1173532-s2.0-85150779890Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Electroanalytical Chemistryinfo:eu-repo/semantics/openAccess2023-07-29T12:57:25Zoai:repositorio.unesp.br:11449/247039Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-07-29T12:57:25Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Applying CeO2 nanorods in flexible electrochemical immunosensor to detect C-reactive protein |
title |
Applying CeO2 nanorods in flexible electrochemical immunosensor to detect C-reactive protein |
spellingShingle |
Applying CeO2 nanorods in flexible electrochemical immunosensor to detect C-reactive protein Amoresi, Rafael Aparecido Ciola [UNESP] CeO2 CRP Immunosensor Nanoparticles |
title_short |
Applying CeO2 nanorods in flexible electrochemical immunosensor to detect C-reactive protein |
title_full |
Applying CeO2 nanorods in flexible electrochemical immunosensor to detect C-reactive protein |
title_fullStr |
Applying CeO2 nanorods in flexible electrochemical immunosensor to detect C-reactive protein |
title_full_unstemmed |
Applying CeO2 nanorods in flexible electrochemical immunosensor to detect C-reactive protein |
title_sort |
Applying CeO2 nanorods in flexible electrochemical immunosensor to detect C-reactive protein |
author |
Amoresi, Rafael Aparecido Ciola [UNESP] |
author_facet |
Amoresi, Rafael Aparecido Ciola [UNESP] Roza, Noemí Angélica Vieira Mazon, Talita |
author_role |
author |
author2 |
Roza, Noemí Angélica Vieira Mazon, Talita |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Federal University of ABC CTI |
dc.contributor.author.fl_str_mv |
Amoresi, Rafael Aparecido Ciola [UNESP] Roza, Noemí Angélica Vieira Mazon, Talita |
dc.subject.por.fl_str_mv |
CeO2 CRP Immunosensor Nanoparticles |
topic |
CeO2 CRP Immunosensor Nanoparticles |
description |
In this work, we report the development of a flexible electrochemical immunosensor based on CeO2 nanorods (NRs) immobilized with C-reactive protein (CRP) antibody, a biomarker of cardiovascular disease. CeO2 NRs were obtained by microwave-assisted hydrothermal method and characterized by XRD, TEM, and XPS. For the preparation of the working electrode, WE, of the immunosensor, the NRs were dispersed in organic solvent and then functionalized with cystamine and glutaraldehyde, and then modified with CRP antibody. The deposition condition was evaluated for electrode functionalization and immobilization. Functionalization and immobilization were verified by spectroscopic techniques and proved effective repeatability and reproducibility. The developed immunosensor had acceptable reproducibility (coefficient of variation = 4.5 %), allowed detection in the range of 0.3 to 7.0 mg L−1 of CRP with a detection limit of 0.18 mg L−1, and applications that are immediately detectable, easy-to-handle, low-cost and ideal for point-of-care. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T12:57:25Z 2023-07-29T12:57:25Z 2023-04-15 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jelechem.2023.117353 Journal of Electroanalytical Chemistry, v. 935. 1572-6657 http://hdl.handle.net/11449/247039 10.1016/j.jelechem.2023.117353 2-s2.0-85150779890 |
url |
http://dx.doi.org/10.1016/j.jelechem.2023.117353 http://hdl.handle.net/11449/247039 |
identifier_str_mv |
Journal of Electroanalytical Chemistry, v. 935. 1572-6657 10.1016/j.jelechem.2023.117353 2-s2.0-85150779890 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Electroanalytical Chemistry |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964940441747456 |