Avaliação do potencial antagônico de leveduras, visando biocontrole de deterioração por penicillium expansum
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.5433/1679-0359.2011v32Suplp1879 http://hdl.handle.net/11449/72930 |
Resumo: | Considerable losses during apple fruit storage occur due to microbiological diseases, mainly caused by Penicillium expansum, which in addition to fruit pulp deterioration produces patulin, a mycotoxin with carcinogenic and teratogenic activity. Biological control of post-harvest disease by antagonist yeasts focused on killer toxins is an appreciable alternative to the chemical fungicides, due to the low possibility of toxic residues demonstrated during fermentative processes. Twenty out of 44 yeasts (16 isolated from fruits, 10 from corn silage and 18 from laboratory anthill), showed antagonism against spores of P. expansum. The assay in solid medium pointed the strongest nutrient competition antagonism by D. hansenii strain C1 (31 mm inhibition diameter), while D. hansenii strain C7 (15 mm) showed higher antibiosis and parasitism pattern. In the following step the extracellular activity was tested performing the assay with culture supernatant in Yeast Medium agar, where C. guilliermondii P3 was more effective against conidia germination (inhibition rate of 58.15%) while P. ohmeri showed better inhibition on micelial growth (66.17%). The antibiosis showed by both yeasts could suggest probable mechanism associated with killer phenomenon, once both strains were killer positive against sensitive reference strains (S. cerevisiae NCYC 1006 and P. kluyveri CAY-15). In order to enhance the production of antifungal substance, these yeasts were cultivated with P. expansum, but the difference between culture supernatant obtained from yeasts cultivated alone and with mould was not significant (P > 0.05). The results demonstrated that the yeasts application constitute a promising tool, enhancing the biological control of P. expansum in post-harvest diseases of apple fruit. |
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Avaliação do potencial antagônico de leveduras, visando biocontrole de deterioração por penicillium expansumEvaluation of potential antagonistism in yeasts, seeking biocontrol of spoilage by penicillium expansumAntagonist yeastsBiocontrolPenicillium expansumConsiderable losses during apple fruit storage occur due to microbiological diseases, mainly caused by Penicillium expansum, which in addition to fruit pulp deterioration produces patulin, a mycotoxin with carcinogenic and teratogenic activity. Biological control of post-harvest disease by antagonist yeasts focused on killer toxins is an appreciable alternative to the chemical fungicides, due to the low possibility of toxic residues demonstrated during fermentative processes. Twenty out of 44 yeasts (16 isolated from fruits, 10 from corn silage and 18 from laboratory anthill), showed antagonism against spores of P. expansum. The assay in solid medium pointed the strongest nutrient competition antagonism by D. hansenii strain C1 (31 mm inhibition diameter), while D. hansenii strain C7 (15 mm) showed higher antibiosis and parasitism pattern. In the following step the extracellular activity was tested performing the assay with culture supernatant in Yeast Medium agar, where C. guilliermondii P3 was more effective against conidia germination (inhibition rate of 58.15%) while P. ohmeri showed better inhibition on micelial growth (66.17%). The antibiosis showed by both yeasts could suggest probable mechanism associated with killer phenomenon, once both strains were killer positive against sensitive reference strains (S. cerevisiae NCYC 1006 and P. kluyveri CAY-15). In order to enhance the production of antifungal substance, these yeasts were cultivated with P. expansum, but the difference between culture supernatant obtained from yeasts cultivated alone and with mould was not significant (P > 0.05). The results demonstrated that the yeasts application constitute a promising tool, enhancing the biological control of P. expansum in post-harvest diseases of apple fruit.Curso de Tecnologia em Alimentos Universidade Tecnológica Federal do Paraná, UTFPR, Francisco Beltrão, PRCiências Biológicas Universidade Estadual de Londrina, UELUniversidade Estadual Paulista, UNESP, Rio Claro, SPUNESP, São José do Rio Preto, SPFaculty of Pharmacy Meijo University, NagoyaCiência de Alimentos UEL, Londrina, PRUniversidade Estadual Paulista, UNESP, Rio Claro, SPUNESP, São José do Rio Preto, SPUniversidade Tecnológica Federal do Paraná, UTFPRUniversidade Estadual de Londrina (UEL)Universidade Estadual Paulista (Unesp)Meijo UniversityCoelho, Alexandre RodrigoDe Andrade Nóbrega, Gisele MariaPagnocca, Fernando Carlos [UNESP]Hoffmann, Fernando Leite [UNESP]Harada, Ken-IchiHirooka, Elisa Yoko2014-05-27T11:26:16Z2014-05-27T11:26:16Z2011-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1879-1892application/pdfhttp://dx.doi.org/10.5433/1679-0359.2011v32Suplp1879Semina:Ciencias Agrarias, v. 32, n. SUPPL. 1, p. 1879-1892, 2011.1679-0359http://hdl.handle.net/11449/7293010.5433/1679-0359.2011v32Suplp18792-s2.0-848561027922-s2.0-84856102792.pdf0654309398298095Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporSemina: Ciências Agrárias0,320info:eu-repo/semantics/openAccess2023-12-01T06:17:22Zoai:repositorio.unesp.br:11449/72930Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-01T06:17:22Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Avaliação do potencial antagônico de leveduras, visando biocontrole de deterioração por penicillium expansum Evaluation of potential antagonistism in yeasts, seeking biocontrol of spoilage by penicillium expansum |
title |
Avaliação do potencial antagônico de leveduras, visando biocontrole de deterioração por penicillium expansum |
spellingShingle |
Avaliação do potencial antagônico de leveduras, visando biocontrole de deterioração por penicillium expansum Coelho, Alexandre Rodrigo Antagonist yeasts Biocontrol Penicillium expansum |
title_short |
Avaliação do potencial antagônico de leveduras, visando biocontrole de deterioração por penicillium expansum |
title_full |
Avaliação do potencial antagônico de leveduras, visando biocontrole de deterioração por penicillium expansum |
title_fullStr |
Avaliação do potencial antagônico de leveduras, visando biocontrole de deterioração por penicillium expansum |
title_full_unstemmed |
Avaliação do potencial antagônico de leveduras, visando biocontrole de deterioração por penicillium expansum |
title_sort |
Avaliação do potencial antagônico de leveduras, visando biocontrole de deterioração por penicillium expansum |
author |
Coelho, Alexandre Rodrigo |
author_facet |
Coelho, Alexandre Rodrigo De Andrade Nóbrega, Gisele Maria Pagnocca, Fernando Carlos [UNESP] Hoffmann, Fernando Leite [UNESP] Harada, Ken-Ichi Hirooka, Elisa Yoko |
author_role |
author |
author2 |
De Andrade Nóbrega, Gisele Maria Pagnocca, Fernando Carlos [UNESP] Hoffmann, Fernando Leite [UNESP] Harada, Ken-Ichi Hirooka, Elisa Yoko |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Tecnológica Federal do Paraná, UTFPR Universidade Estadual de Londrina (UEL) Universidade Estadual Paulista (Unesp) Meijo University |
dc.contributor.author.fl_str_mv |
Coelho, Alexandre Rodrigo De Andrade Nóbrega, Gisele Maria Pagnocca, Fernando Carlos [UNESP] Hoffmann, Fernando Leite [UNESP] Harada, Ken-Ichi Hirooka, Elisa Yoko |
dc.subject.por.fl_str_mv |
Antagonist yeasts Biocontrol Penicillium expansum |
topic |
Antagonist yeasts Biocontrol Penicillium expansum |
description |
Considerable losses during apple fruit storage occur due to microbiological diseases, mainly caused by Penicillium expansum, which in addition to fruit pulp deterioration produces patulin, a mycotoxin with carcinogenic and teratogenic activity. Biological control of post-harvest disease by antagonist yeasts focused on killer toxins is an appreciable alternative to the chemical fungicides, due to the low possibility of toxic residues demonstrated during fermentative processes. Twenty out of 44 yeasts (16 isolated from fruits, 10 from corn silage and 18 from laboratory anthill), showed antagonism against spores of P. expansum. The assay in solid medium pointed the strongest nutrient competition antagonism by D. hansenii strain C1 (31 mm inhibition diameter), while D. hansenii strain C7 (15 mm) showed higher antibiosis and parasitism pattern. In the following step the extracellular activity was tested performing the assay with culture supernatant in Yeast Medium agar, where C. guilliermondii P3 was more effective against conidia germination (inhibition rate of 58.15%) while P. ohmeri showed better inhibition on micelial growth (66.17%). The antibiosis showed by both yeasts could suggest probable mechanism associated with killer phenomenon, once both strains were killer positive against sensitive reference strains (S. cerevisiae NCYC 1006 and P. kluyveri CAY-15). In order to enhance the production of antifungal substance, these yeasts were cultivated with P. expansum, but the difference between culture supernatant obtained from yeasts cultivated alone and with mould was not significant (P > 0.05). The results demonstrated that the yeasts application constitute a promising tool, enhancing the biological control of P. expansum in post-harvest diseases of apple fruit. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-12-01 2014-05-27T11:26:16Z 2014-05-27T11:26:16Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.5433/1679-0359.2011v32Suplp1879 Semina:Ciencias Agrarias, v. 32, n. SUPPL. 1, p. 1879-1892, 2011. 1679-0359 http://hdl.handle.net/11449/72930 10.5433/1679-0359.2011v32Suplp1879 2-s2.0-84856102792 2-s2.0-84856102792.pdf 0654309398298095 |
url |
http://dx.doi.org/10.5433/1679-0359.2011v32Suplp1879 http://hdl.handle.net/11449/72930 |
identifier_str_mv |
Semina:Ciencias Agrarias, v. 32, n. SUPPL. 1, p. 1879-1892, 2011. 1679-0359 10.5433/1679-0359.2011v32Suplp1879 2-s2.0-84856102792 2-s2.0-84856102792.pdf 0654309398298095 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Semina: Ciências Agrárias 0,320 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1879-1892 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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