Diversity of piroplasmids among wild and domestic mammals and ectoparasites in Pantanal wetland, Brazil
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.ttbdis.2017.09.010 http://hdl.handle.net/11449/170140 |
Resumo: | Piroplasmoses are one of the most prevalent arthropod-borne diseases of animals. The present work aimed to investigate the occurrence of piroplasmid in wild mammals, domestic dogs and ectoparasites in southern Pantanal region, central-western Brazil. For that purpose, blood or tissue samples from 31 Nasua nasua, 78 Cerdocyon thous, 7 Leopardus pardalis, 42 dogs, 110 wild rodents, and 30 marsupials, and 1582 ticks were submitted to PCR assays for piroplasmid targeting 18SrRNA and hps70 genes. Seven dogs, one C. thous, five L. pardalis, three N. nasua, six wild rodents, eight Amblyomma parvum, two Amblyomma sculptum and one Amblyomma ovale were positive for piroplasmid-PCR assays. Genotypes closely related to Babesia vogeli were detected in six dogs and five wild rodents. While genotypes closely related to Babesia caballi were detected in one C. thous, one dog, one A. ovale and one A. sculptum, genotypes closely related to Babesia bigemina and Babesia bovis were detected in four A. parvum ticks. Four sequences obtained from A. parvum, three coatis and one wild rodent were closely related to Theileria equi. Cytauxzoon spp. was detected in four ocelots. The present study revealed that wild and domestic animals in Brazilian southern Pantanal are exposed to different piroplasmid species. |
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Diversity of piroplasmids among wild and domestic mammals and ectoparasites in Pantanal wetland, BrazilBabesiaCytauxzoonDogsPhylogenetic analysisRangeliaTheileriaWildlifePiroplasmoses are one of the most prevalent arthropod-borne diseases of animals. The present work aimed to investigate the occurrence of piroplasmid in wild mammals, domestic dogs and ectoparasites in southern Pantanal region, central-western Brazil. For that purpose, blood or tissue samples from 31 Nasua nasua, 78 Cerdocyon thous, 7 Leopardus pardalis, 42 dogs, 110 wild rodents, and 30 marsupials, and 1582 ticks were submitted to PCR assays for piroplasmid targeting 18SrRNA and hps70 genes. Seven dogs, one C. thous, five L. pardalis, three N. nasua, six wild rodents, eight Amblyomma parvum, two Amblyomma sculptum and one Amblyomma ovale were positive for piroplasmid-PCR assays. Genotypes closely related to Babesia vogeli were detected in six dogs and five wild rodents. While genotypes closely related to Babesia caballi were detected in one C. thous, one dog, one A. ovale and one A. sculptum, genotypes closely related to Babesia bigemina and Babesia bovis were detected in four A. parvum ticks. Four sequences obtained from A. parvum, three coatis and one wild rodent were closely related to Theileria equi. Cytauxzoon spp. was detected in four ocelots. The present study revealed that wild and domestic animals in Brazilian southern Pantanal are exposed to different piroplasmid species.Universidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabal, SP, BrazilUniversidade Católica Dom Bosco, Campo Grande, MS, BrazilImunodot Diagnósticos Ltda, Jaboticabal, SP, BrazilUniversidade Estadual Paulista (Unesp)de Sousa, Keyla Carstens MarquesFernandes, Marina PugnaghiHerrera, Heitor MiragliaFreschi, Carla RobertaMachado, Rosangela ZacariasAndré, Marcos Rogério2018-12-11T16:49:26Z2018-12-11T16:49:26Z2017-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1016/j.ttbdis.2017.09.010Ticks and Tick-borne Diseases.1877-96031877-959Xhttp://hdl.handle.net/11449/17014010.1016/j.ttbdis.2017.09.0102-s2.0-850295930522-s2.0-85029593052.pdf9139899895580513Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTicks and Tick-borne Diseases1,421info:eu-repo/semantics/openAccess2023-10-23T06:08:36Zoai:repositorio.unesp.br:11449/170140Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-23T06:08:36Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Diversity of piroplasmids among wild and domestic mammals and ectoparasites in Pantanal wetland, Brazil |
title |
Diversity of piroplasmids among wild and domestic mammals and ectoparasites in Pantanal wetland, Brazil |
spellingShingle |
Diversity of piroplasmids among wild and domestic mammals and ectoparasites in Pantanal wetland, Brazil de Sousa, Keyla Carstens Marques Babesia Cytauxzoon Dogs Phylogenetic analysis Rangelia Theileria Wildlife |
title_short |
Diversity of piroplasmids among wild and domestic mammals and ectoparasites in Pantanal wetland, Brazil |
title_full |
Diversity of piroplasmids among wild and domestic mammals and ectoparasites in Pantanal wetland, Brazil |
title_fullStr |
Diversity of piroplasmids among wild and domestic mammals and ectoparasites in Pantanal wetland, Brazil |
title_full_unstemmed |
Diversity of piroplasmids among wild and domestic mammals and ectoparasites in Pantanal wetland, Brazil |
title_sort |
Diversity of piroplasmids among wild and domestic mammals and ectoparasites in Pantanal wetland, Brazil |
author |
de Sousa, Keyla Carstens Marques |
author_facet |
de Sousa, Keyla Carstens Marques Fernandes, Marina Pugnaghi Herrera, Heitor Miraglia Freschi, Carla Roberta Machado, Rosangela Zacarias André, Marcos Rogério |
author_role |
author |
author2 |
Fernandes, Marina Pugnaghi Herrera, Heitor Miraglia Freschi, Carla Roberta Machado, Rosangela Zacarias André, Marcos Rogério |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
de Sousa, Keyla Carstens Marques Fernandes, Marina Pugnaghi Herrera, Heitor Miraglia Freschi, Carla Roberta Machado, Rosangela Zacarias André, Marcos Rogério |
dc.subject.por.fl_str_mv |
Babesia Cytauxzoon Dogs Phylogenetic analysis Rangelia Theileria Wildlife |
topic |
Babesia Cytauxzoon Dogs Phylogenetic analysis Rangelia Theileria Wildlife |
description |
Piroplasmoses are one of the most prevalent arthropod-borne diseases of animals. The present work aimed to investigate the occurrence of piroplasmid in wild mammals, domestic dogs and ectoparasites in southern Pantanal region, central-western Brazil. For that purpose, blood or tissue samples from 31 Nasua nasua, 78 Cerdocyon thous, 7 Leopardus pardalis, 42 dogs, 110 wild rodents, and 30 marsupials, and 1582 ticks were submitted to PCR assays for piroplasmid targeting 18SrRNA and hps70 genes. Seven dogs, one C. thous, five L. pardalis, three N. nasua, six wild rodents, eight Amblyomma parvum, two Amblyomma sculptum and one Amblyomma ovale were positive for piroplasmid-PCR assays. Genotypes closely related to Babesia vogeli were detected in six dogs and five wild rodents. While genotypes closely related to Babesia caballi were detected in one C. thous, one dog, one A. ovale and one A. sculptum, genotypes closely related to Babesia bigemina and Babesia bovis were detected in four A. parvum ticks. Four sequences obtained from A. parvum, three coatis and one wild rodent were closely related to Theileria equi. Cytauxzoon spp. was detected in four ocelots. The present study revealed that wild and domestic animals in Brazilian southern Pantanal are exposed to different piroplasmid species. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-01-01 2018-12-11T16:49:26Z 2018-12-11T16:49:26Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.ttbdis.2017.09.010 Ticks and Tick-borne Diseases. 1877-9603 1877-959X http://hdl.handle.net/11449/170140 10.1016/j.ttbdis.2017.09.010 2-s2.0-85029593052 2-s2.0-85029593052.pdf 9139899895580513 |
url |
http://dx.doi.org/10.1016/j.ttbdis.2017.09.010 http://hdl.handle.net/11449/170140 |
identifier_str_mv |
Ticks and Tick-borne Diseases. 1877-9603 1877-959X 10.1016/j.ttbdis.2017.09.010 2-s2.0-85029593052 2-s2.0-85029593052.pdf 9139899895580513 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Ticks and Tick-borne Diseases 1,421 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1797789485978615808 |