Development and characterization of microsatellite loci of microglanis cottoides (siluriformes: pseudopimelodidae) and cross-species amplification
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1679-62252013000300011 http://hdl.handle.net/11449/111500 |
Resumo: | Thirteen microsatellite loci were isolated and characterized in Microglanis cottoides. Of these, two were monomorphic and 11 were polymorphic. These polymorphic loci tested on 24 individuals from a wild population produced a total of 108 different alleles, with levels of variability high, ranging from 2 to 20, with an average of 8.3 alleles per locus. The observed and expected heterozygosity ranged from 0.125 to 0.958 and from 0.119 to 0.931, respectively. A high combined probability of paternity exclusion value and a low probability combined genetic identity value obtained show that the set of loci described herein displays good suitability for paternity studies and differentiation of M. cottoides. Additionally, all thirteen microsatellite primers developed for M. cottoides were tested in four other Pseudopimelodidae species and successful cross-species amplification was achieved for the majority of loci. |
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Development and characterization of microsatellite loci of microglanis cottoides (siluriformes: pseudopimelodidae) and cross-species amplificationBrazilian Eastern basinCatfishEnriched genomic libraryGenetic markerSSRThirteen microsatellite loci were isolated and characterized in Microglanis cottoides. Of these, two were monomorphic and 11 were polymorphic. These polymorphic loci tested on 24 individuals from a wild population produced a total of 108 different alleles, with levels of variability high, ranging from 2 to 20, with an average of 8.3 alleles per locus. The observed and expected heterozygosity ranged from 0.125 to 0.958 and from 0.119 to 0.931, respectively. A high combined probability of paternity exclusion value and a low probability combined genetic identity value obtained show that the set of loci described herein displays good suitability for paternity studies and differentiation of M. cottoides. Additionally, all thirteen microsatellite primers developed for M. cottoides were tested in four other Pseudopimelodidae species and successful cross-species amplification was achieved for the majority of loci.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Programa UniversalUniversidade Estadual de LondrinaIBAMA/SISBIOUniv Estadual Londrina, Dept Biol Anim & Vegetal, Ctr Ciencias Biol, Programa Posgrad Ciencias Biol, BR-86051990 Londrina, PR, BrazilUniv Estadual Londrina, Dept Biol Geral, Ctr Ciencias Biol, Programa Posgrad Genet & Biol Mol, BR-86051990 Londrina, PR, BrazilUniv Paulista Julio Mesquita Filho, Inst Biociencias, Dept Biociencias, BR-18618970 Botucatu, SP, BrazilUniv Paulista Julio Mesquita Filho, Inst Biociencias, Dept Biociencias, BR-18618970 Botucatu, SP, BrazilPrograma Universal05/2011Programa Universal22.633IBAMA/SISBIO12120-1Soc Brasileira IctiologiaUniversidade Estadual de Londrina (UEL)Universidade Estadual Paulista (Unesp)Souza-Shibatta, LeniceFerreira, Dhiego GomesOliveira, Claudio [UNESP]Almeida, Fernanda Simoes deShibatta, Oscar AkioSofia, Silvia Helena2014-12-03T13:08:42Z2014-12-03T13:08:42Z2013-06-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article581-585application/pdfhttp://dx.doi.org/10.1590/S1679-62252013000300011Neotropical Ichthyology. São Paulo: Soc Brasileira Ictiologia, v. 11, n. 3, p. 581-585, 2013.1679-6225http://hdl.handle.net/11449/111500S1679-62252013000300011WOS:000326973700011S1679-62252013000300011.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengNeotropical Ichthyology1.2160,559info:eu-repo/semantics/openAccess2024-01-09T06:29:16Zoai:repositorio.unesp.br:11449/111500Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-09T06:29:16Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Development and characterization of microsatellite loci of microglanis cottoides (siluriformes: pseudopimelodidae) and cross-species amplification |
title |
Development and characterization of microsatellite loci of microglanis cottoides (siluriformes: pseudopimelodidae) and cross-species amplification |
spellingShingle |
Development and characterization of microsatellite loci of microglanis cottoides (siluriformes: pseudopimelodidae) and cross-species amplification Souza-Shibatta, Lenice Brazilian Eastern basin Catfish Enriched genomic library Genetic marker SSR |
title_short |
Development and characterization of microsatellite loci of microglanis cottoides (siluriformes: pseudopimelodidae) and cross-species amplification |
title_full |
Development and characterization of microsatellite loci of microglanis cottoides (siluriformes: pseudopimelodidae) and cross-species amplification |
title_fullStr |
Development and characterization of microsatellite loci of microglanis cottoides (siluriformes: pseudopimelodidae) and cross-species amplification |
title_full_unstemmed |
Development and characterization of microsatellite loci of microglanis cottoides (siluriformes: pseudopimelodidae) and cross-species amplification |
title_sort |
Development and characterization of microsatellite loci of microglanis cottoides (siluriformes: pseudopimelodidae) and cross-species amplification |
author |
Souza-Shibatta, Lenice |
author_facet |
Souza-Shibatta, Lenice Ferreira, Dhiego Gomes Oliveira, Claudio [UNESP] Almeida, Fernanda Simoes de Shibatta, Oscar Akio Sofia, Silvia Helena |
author_role |
author |
author2 |
Ferreira, Dhiego Gomes Oliveira, Claudio [UNESP] Almeida, Fernanda Simoes de Shibatta, Oscar Akio Sofia, Silvia Helena |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Londrina (UEL) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Souza-Shibatta, Lenice Ferreira, Dhiego Gomes Oliveira, Claudio [UNESP] Almeida, Fernanda Simoes de Shibatta, Oscar Akio Sofia, Silvia Helena |
dc.subject.por.fl_str_mv |
Brazilian Eastern basin Catfish Enriched genomic library Genetic marker SSR |
topic |
Brazilian Eastern basin Catfish Enriched genomic library Genetic marker SSR |
description |
Thirteen microsatellite loci were isolated and characterized in Microglanis cottoides. Of these, two were monomorphic and 11 were polymorphic. These polymorphic loci tested on 24 individuals from a wild population produced a total of 108 different alleles, with levels of variability high, ranging from 2 to 20, with an average of 8.3 alleles per locus. The observed and expected heterozygosity ranged from 0.125 to 0.958 and from 0.119 to 0.931, respectively. A high combined probability of paternity exclusion value and a low probability combined genetic identity value obtained show that the set of loci described herein displays good suitability for paternity studies and differentiation of M. cottoides. Additionally, all thirteen microsatellite primers developed for M. cottoides were tested in four other Pseudopimelodidae species and successful cross-species amplification was achieved for the majority of loci. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-06-30 2014-12-03T13:08:42Z 2014-12-03T13:08:42Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1679-62252013000300011 Neotropical Ichthyology. São Paulo: Soc Brasileira Ictiologia, v. 11, n. 3, p. 581-585, 2013. 1679-6225 http://hdl.handle.net/11449/111500 S1679-62252013000300011 WOS:000326973700011 S1679-62252013000300011.pdf |
url |
http://dx.doi.org/10.1590/S1679-62252013000300011 http://hdl.handle.net/11449/111500 |
identifier_str_mv |
Neotropical Ichthyology. São Paulo: Soc Brasileira Ictiologia, v. 11, n. 3, p. 581-585, 2013. 1679-6225 S1679-62252013000300011 WOS:000326973700011 S1679-62252013000300011.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Neotropical Ichthyology 1.216 0,559 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
581-585 application/pdf |
dc.publisher.none.fl_str_mv |
Soc Brasileira Ictiologia |
publisher.none.fl_str_mv |
Soc Brasileira Ictiologia |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965569595736064 |