Influence of ceramic veneer on the transdentinal cytotoxicity, degree of conversion and bond strength of light-cured resin cements to dentin
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.dental.2022.04.015 http://hdl.handle.net/11449/241836 |
Resumo: | Objectives: To investigate the transdentinal cytotoxicity (TC), degree of conversion (DC), and micro shear bond strength (μSBS) to dentin of light-cured resin cements (LCRCs) photoactivated directly or through a ceramic veneer( ± CV). Materials and methods: The TC was assessed using human dentin discs adapted into artificial pulp chambers. Odontoblast-like cells were seeded on the pulp surface of the discs, then three LCRCs( ± CV) were applied on their etched and hybridized occlusal surface (n = 8/group). The adhesive systems of each LCRCs and sterile phosphate-buffered saline were used as positive and negative controls, respectively. After 24 h, the viability and morphology of cells adhered on discs were assessed. The extracts (culture medium + components of the materials diffused through the discs) were applied on the MDPC-23 to evaluate their viability, adhesion/spreading (A/S), alkaline phosphatase activity (ALP), and mineralized nodule formation (MN). LCRCs( ± CV) specimens were evaluated concerning the DC and μSBS to dentin. Data were analyzed by one-, two-, or three-way ANOVA/Dunnett, Sidak, and Games-Howell tests (α = 5%). Results: All LCRCs( ± CV) reduced cell viability, A/S, ALP, MN, and DC. Except for μSBS, the intensity of reduction was dependent on the LCRC used. LCRCs+CV resulted in lower DC and μSBS but did not increase the TC. Significance: Besides the presence of CV between the light source and LCRCs reduces the degree of conversion and bond strength to dentin, these materials cause variable level of transdentinal toxicity to pulp cells. Thus, the composition and curing protocols of LCRCs should be revisited and reinforced to prevent mechanical and biological drawbacks. |
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Influence of ceramic veneer on the transdentinal cytotoxicity, degree of conversion and bond strength of light-cured resin cements to dentinBond strengthCeramic veneerCytotoxicityDegree of conversionLight–cured resin cementOdontoblastObjectives: To investigate the transdentinal cytotoxicity (TC), degree of conversion (DC), and micro shear bond strength (μSBS) to dentin of light-cured resin cements (LCRCs) photoactivated directly or through a ceramic veneer( ± CV). Materials and methods: The TC was assessed using human dentin discs adapted into artificial pulp chambers. Odontoblast-like cells were seeded on the pulp surface of the discs, then three LCRCs( ± CV) were applied on their etched and hybridized occlusal surface (n = 8/group). The adhesive systems of each LCRCs and sterile phosphate-buffered saline were used as positive and negative controls, respectively. After 24 h, the viability and morphology of cells adhered on discs were assessed. The extracts (culture medium + components of the materials diffused through the discs) were applied on the MDPC-23 to evaluate their viability, adhesion/spreading (A/S), alkaline phosphatase activity (ALP), and mineralized nodule formation (MN). LCRCs( ± CV) specimens were evaluated concerning the DC and μSBS to dentin. Data were analyzed by one-, two-, or three-way ANOVA/Dunnett, Sidak, and Games-Howell tests (α = 5%). Results: All LCRCs( ± CV) reduced cell viability, A/S, ALP, MN, and DC. Except for μSBS, the intensity of reduction was dependent on the LCRC used. LCRCs+CV resulted in lower DC and μSBS but did not increase the TC. Significance: Besides the presence of CV between the light source and LCRCs reduces the degree of conversion and bond strength to dentin, these materials cause variable level of transdentinal toxicity to pulp cells. Thus, the composition and curing protocols of LCRCs should be revisited and reinforced to prevent mechanical and biological drawbacks.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Department of Dental Materials and Prosthodontics São Paulo State University (UNESP) School of Dentistry, SPDepartment of Genetics Morphology Orthodontics and Pediatric Dentistry São Paulo State University (UNESP) School of Dentistry, SPDepartment of Physiology and Pathology São Paulo State University (UNESP) School of Dentistry, SPDepartment of Dental Materials and Prosthodontics São Paulo State University (UNESP) School of Dentistry, SPDepartment of Genetics Morphology Orthodontics and Pediatric Dentistry São Paulo State University (UNESP) School of Dentistry, SPDepartment of Physiology and Pathology São Paulo State University (UNESP) School of Dentistry, SPFAPESP: 2017/22739–9CNPq: 302047/2019–0CNPq: 408721/2018–9CAPES: 88887.513794/2020–00Universidade Estadual Paulista (UNESP)Kitagawa, Fernanda Ali [UNESP]Leite, Maria Luísa [UNESP]Soares, Igor Paulino Mendes [UNESP]Anselmi, Caroline [UNESP]de Oliveira Ribeiro, Rafael Antônio [UNESP]Hebling, Josimeri [UNESP]de Souza Costa, Carlos Alberto [UNESP]2023-03-02T00:30:02Z2023-03-02T00:30:02Z2022-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlee160-e173http://dx.doi.org/10.1016/j.dental.2022.04.015Dental Materials, v. 38, n. 6, p. e160-e173, 2022.0109-5641http://hdl.handle.net/11449/24183610.1016/j.dental.2022.04.0152-s2.0-85130016084Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengDental Materialsinfo:eu-repo/semantics/openAccess2023-03-02T00:30:02Zoai:repositorio.unesp.br:11449/241836Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-03-02T00:30:02Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Influence of ceramic veneer on the transdentinal cytotoxicity, degree of conversion and bond strength of light-cured resin cements to dentin |
title |
Influence of ceramic veneer on the transdentinal cytotoxicity, degree of conversion and bond strength of light-cured resin cements to dentin |
spellingShingle |
Influence of ceramic veneer on the transdentinal cytotoxicity, degree of conversion and bond strength of light-cured resin cements to dentin Kitagawa, Fernanda Ali [UNESP] Bond strength Ceramic veneer Cytotoxicity Degree of conversion Light–cured resin cement Odontoblast |
title_short |
Influence of ceramic veneer on the transdentinal cytotoxicity, degree of conversion and bond strength of light-cured resin cements to dentin |
title_full |
Influence of ceramic veneer on the transdentinal cytotoxicity, degree of conversion and bond strength of light-cured resin cements to dentin |
title_fullStr |
Influence of ceramic veneer on the transdentinal cytotoxicity, degree of conversion and bond strength of light-cured resin cements to dentin |
title_full_unstemmed |
Influence of ceramic veneer on the transdentinal cytotoxicity, degree of conversion and bond strength of light-cured resin cements to dentin |
title_sort |
Influence of ceramic veneer on the transdentinal cytotoxicity, degree of conversion and bond strength of light-cured resin cements to dentin |
author |
Kitagawa, Fernanda Ali [UNESP] |
author_facet |
Kitagawa, Fernanda Ali [UNESP] Leite, Maria Luísa [UNESP] Soares, Igor Paulino Mendes [UNESP] Anselmi, Caroline [UNESP] de Oliveira Ribeiro, Rafael Antônio [UNESP] Hebling, Josimeri [UNESP] de Souza Costa, Carlos Alberto [UNESP] |
author_role |
author |
author2 |
Leite, Maria Luísa [UNESP] Soares, Igor Paulino Mendes [UNESP] Anselmi, Caroline [UNESP] de Oliveira Ribeiro, Rafael Antônio [UNESP] Hebling, Josimeri [UNESP] de Souza Costa, Carlos Alberto [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Kitagawa, Fernanda Ali [UNESP] Leite, Maria Luísa [UNESP] Soares, Igor Paulino Mendes [UNESP] Anselmi, Caroline [UNESP] de Oliveira Ribeiro, Rafael Antônio [UNESP] Hebling, Josimeri [UNESP] de Souza Costa, Carlos Alberto [UNESP] |
dc.subject.por.fl_str_mv |
Bond strength Ceramic veneer Cytotoxicity Degree of conversion Light–cured resin cement Odontoblast |
topic |
Bond strength Ceramic veneer Cytotoxicity Degree of conversion Light–cured resin cement Odontoblast |
description |
Objectives: To investigate the transdentinal cytotoxicity (TC), degree of conversion (DC), and micro shear bond strength (μSBS) to dentin of light-cured resin cements (LCRCs) photoactivated directly or through a ceramic veneer( ± CV). Materials and methods: The TC was assessed using human dentin discs adapted into artificial pulp chambers. Odontoblast-like cells were seeded on the pulp surface of the discs, then three LCRCs( ± CV) were applied on their etched and hybridized occlusal surface (n = 8/group). The adhesive systems of each LCRCs and sterile phosphate-buffered saline were used as positive and negative controls, respectively. After 24 h, the viability and morphology of cells adhered on discs were assessed. The extracts (culture medium + components of the materials diffused through the discs) were applied on the MDPC-23 to evaluate their viability, adhesion/spreading (A/S), alkaline phosphatase activity (ALP), and mineralized nodule formation (MN). LCRCs( ± CV) specimens were evaluated concerning the DC and μSBS to dentin. Data were analyzed by one-, two-, or three-way ANOVA/Dunnett, Sidak, and Games-Howell tests (α = 5%). Results: All LCRCs( ± CV) reduced cell viability, A/S, ALP, MN, and DC. Except for μSBS, the intensity of reduction was dependent on the LCRC used. LCRCs+CV resulted in lower DC and μSBS but did not increase the TC. Significance: Besides the presence of CV between the light source and LCRCs reduces the degree of conversion and bond strength to dentin, these materials cause variable level of transdentinal toxicity to pulp cells. Thus, the composition and curing protocols of LCRCs should be revisited and reinforced to prevent mechanical and biological drawbacks. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-06-01 2023-03-02T00:30:02Z 2023-03-02T00:30:02Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.dental.2022.04.015 Dental Materials, v. 38, n. 6, p. e160-e173, 2022. 0109-5641 http://hdl.handle.net/11449/241836 10.1016/j.dental.2022.04.015 2-s2.0-85130016084 |
url |
http://dx.doi.org/10.1016/j.dental.2022.04.015 http://hdl.handle.net/11449/241836 |
identifier_str_mv |
Dental Materials, v. 38, n. 6, p. e160-e173, 2022. 0109-5641 10.1016/j.dental.2022.04.015 2-s2.0-85130016084 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Dental Materials |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
e160-e173 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964736320700416 |