High sperm concentration during cryopreservation decreases post-thaw motility percentage without compromising in vitro fertilization outcomes in common carp
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.aquaculture.2022.738746 http://hdl.handle.net/11449/247598 |
Resumo: | To avoid mechanical compression of spermatozoa during the cryopreservation, a high dilution ratio with the cryoprotective medium is applied. Due to this, the sample volume increases, which entails increased cryobank capacity. Moreover, the high volume of cryomedias does not allow to fertilize of large volumes of eggs in practical artificial reproduction of common carp aquaculture. The current study demonstrated that reasonable lowering of dilution rates might still be effective for carp sperm cryobanking —elevation of spermatozoa concentration up to 13 × 109 spz mL−1 resulted in a significant decrease in post-thaw sperm motility percentage to 20% compared with 39% in 0.5 × 109 spz mL−1. Nevertheless, no significant differences in sperm kinetic parameters (VCL and LIN) were found in this case. The fertilization outcome (embryo development and hatching rates) was similar after applying thawed sperm samples with “optimal” (2.2–2.4 × 109 spz mL−1) and “sub-optimal” (11.0–13.0 × 109 spz mL−1) concentrations (sperm/egg ratio at fertilization was in the range of 0.3–4.5 × 105 spz/egg). Thus, applying a low dilution rate such as one part of sperm to one of the cryoprotective mediums is favorable for decreasing cryo-storage space and the sperm volume needed to fertilize big egg numbers. The experiment also shows that the 4.5 × 105 spz/egg ratio is not sufficient for good fertilization, and it is necessary to use higher sperm concentrations per egg or improve the method of fertilization. |
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High sperm concentration during cryopreservation decreases post-thaw motility percentage without compromising in vitro fertilization outcomes in common carpComputer-assisted sperm analysisCryopreservationCypriniformesSperm concentrationSperm motilitySperm spectrophotometryTo avoid mechanical compression of spermatozoa during the cryopreservation, a high dilution ratio with the cryoprotective medium is applied. Due to this, the sample volume increases, which entails increased cryobank capacity. Moreover, the high volume of cryomedias does not allow to fertilize of large volumes of eggs in practical artificial reproduction of common carp aquaculture. The current study demonstrated that reasonable lowering of dilution rates might still be effective for carp sperm cryobanking —elevation of spermatozoa concentration up to 13 × 109 spz mL−1 resulted in a significant decrease in post-thaw sperm motility percentage to 20% compared with 39% in 0.5 × 109 spz mL−1. Nevertheless, no significant differences in sperm kinetic parameters (VCL and LIN) were found in this case. The fertilization outcome (embryo development and hatching rates) was similar after applying thawed sperm samples with “optimal” (2.2–2.4 × 109 spz mL−1) and “sub-optimal” (11.0–13.0 × 109 spz mL−1) concentrations (sperm/egg ratio at fertilization was in the range of 0.3–4.5 × 105 spz/egg). Thus, applying a low dilution rate such as one part of sperm to one of the cryoprotective mediums is favorable for decreasing cryo-storage space and the sperm volume needed to fertilize big egg numbers. The experiment also shows that the 4.5 × 105 spz/egg ratio is not sufficient for good fertilization, and it is necessary to use higher sperm concentrations per egg or improve the method of fertilization.Grantová Agentura České RepublikyMinisterstvo ZemědělstvíUniversity of South Bohemia in České Budějovice Faculty of Fisheries and Protection of Waters South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses Research Institute of Fish Culture and Hydrobiology, Zátiší 728/IIUNESP –Paulista State University “Júlio de Mesquita Filho” Ilha Solteira Campus Department of Biology and Zootechnics LINEO - Neotropical Ichthyology Laboratory - Rua Monção, 226 - North Zone, São PauloPost-graduate Program in Biological Sciences (Zoology) - R. Prof. Dr. Antônio Celso Wagner Zanin, 250 - Rubião Junior District, São PauloUNESP –Paulista State University “Júlio de Mesquita Filho” Ilha Solteira Campus Department of Biology and Zootechnics LINEO - Neotropical Ichthyology Laboratory - Rua Monção, 226 - North Zone, São PauloGrantová Agentura České Republiky: N 22-14069SMinisterstvo Zemědělství: NAZV QK21010141Research Institute of Fish Culture and HydrobiologyUniversidade Estadual Paulista (UNESP)Sotnikov, AnatoliiRodina, MarekStechkina, TaisiyaBenevente, Cristiane Fernanda [UNESP]Gela, DavidBoryshpolets, SergiiKholodnyy, VitaliyLinhart, OtomarDzyuba, Borys2023-07-29T13:20:21Z2023-07-29T13:20:21Z2023-01-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.aquaculture.2022.738746Aquaculture, v. 562.0044-8486http://hdl.handle.net/11449/24759810.1016/j.aquaculture.2022.7387462-s2.0-85137050969Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAquacultureinfo:eu-repo/semantics/openAccess2023-07-29T13:20:21Zoai:repositorio.unesp.br:11449/247598Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-07-29T13:20:21Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
High sperm concentration during cryopreservation decreases post-thaw motility percentage without compromising in vitro fertilization outcomes in common carp |
title |
High sperm concentration during cryopreservation decreases post-thaw motility percentage without compromising in vitro fertilization outcomes in common carp |
spellingShingle |
High sperm concentration during cryopreservation decreases post-thaw motility percentage without compromising in vitro fertilization outcomes in common carp Sotnikov, Anatolii Computer-assisted sperm analysis Cryopreservation Cypriniformes Sperm concentration Sperm motility Sperm spectrophotometry |
title_short |
High sperm concentration during cryopreservation decreases post-thaw motility percentage without compromising in vitro fertilization outcomes in common carp |
title_full |
High sperm concentration during cryopreservation decreases post-thaw motility percentage without compromising in vitro fertilization outcomes in common carp |
title_fullStr |
High sperm concentration during cryopreservation decreases post-thaw motility percentage without compromising in vitro fertilization outcomes in common carp |
title_full_unstemmed |
High sperm concentration during cryopreservation decreases post-thaw motility percentage without compromising in vitro fertilization outcomes in common carp |
title_sort |
High sperm concentration during cryopreservation decreases post-thaw motility percentage without compromising in vitro fertilization outcomes in common carp |
author |
Sotnikov, Anatolii |
author_facet |
Sotnikov, Anatolii Rodina, Marek Stechkina, Taisiya Benevente, Cristiane Fernanda [UNESP] Gela, David Boryshpolets, Sergii Kholodnyy, Vitaliy Linhart, Otomar Dzyuba, Borys |
author_role |
author |
author2 |
Rodina, Marek Stechkina, Taisiya Benevente, Cristiane Fernanda [UNESP] Gela, David Boryshpolets, Sergii Kholodnyy, Vitaliy Linhart, Otomar Dzyuba, Borys |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Research Institute of Fish Culture and Hydrobiology Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Sotnikov, Anatolii Rodina, Marek Stechkina, Taisiya Benevente, Cristiane Fernanda [UNESP] Gela, David Boryshpolets, Sergii Kholodnyy, Vitaliy Linhart, Otomar Dzyuba, Borys |
dc.subject.por.fl_str_mv |
Computer-assisted sperm analysis Cryopreservation Cypriniformes Sperm concentration Sperm motility Sperm spectrophotometry |
topic |
Computer-assisted sperm analysis Cryopreservation Cypriniformes Sperm concentration Sperm motility Sperm spectrophotometry |
description |
To avoid mechanical compression of spermatozoa during the cryopreservation, a high dilution ratio with the cryoprotective medium is applied. Due to this, the sample volume increases, which entails increased cryobank capacity. Moreover, the high volume of cryomedias does not allow to fertilize of large volumes of eggs in practical artificial reproduction of common carp aquaculture. The current study demonstrated that reasonable lowering of dilution rates might still be effective for carp sperm cryobanking —elevation of spermatozoa concentration up to 13 × 109 spz mL−1 resulted in a significant decrease in post-thaw sperm motility percentage to 20% compared with 39% in 0.5 × 109 spz mL−1. Nevertheless, no significant differences in sperm kinetic parameters (VCL and LIN) were found in this case. The fertilization outcome (embryo development and hatching rates) was similar after applying thawed sperm samples with “optimal” (2.2–2.4 × 109 spz mL−1) and “sub-optimal” (11.0–13.0 × 109 spz mL−1) concentrations (sperm/egg ratio at fertilization was in the range of 0.3–4.5 × 105 spz/egg). Thus, applying a low dilution rate such as one part of sperm to one of the cryoprotective mediums is favorable for decreasing cryo-storage space and the sperm volume needed to fertilize big egg numbers. The experiment also shows that the 4.5 × 105 spz/egg ratio is not sufficient for good fertilization, and it is necessary to use higher sperm concentrations per egg or improve the method of fertilization. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T13:20:21Z 2023-07-29T13:20:21Z 2023-01-15 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.aquaculture.2022.738746 Aquaculture, v. 562. 0044-8486 http://hdl.handle.net/11449/247598 10.1016/j.aquaculture.2022.738746 2-s2.0-85137050969 |
url |
http://dx.doi.org/10.1016/j.aquaculture.2022.738746 http://hdl.handle.net/11449/247598 |
identifier_str_mv |
Aquaculture, v. 562. 0044-8486 10.1016/j.aquaculture.2022.738746 2-s2.0-85137050969 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Aquaculture |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1797789697091567616 |