Antimicrobial and biofilm anti-adhesion activities of silver nanoparticles and farnesol against endodontic microorganisms for possible application in root canal treatment
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.archoralbio.2019.104481 http://hdl.handle.net/11449/190515 |
Resumo: | Objective: This study aimed to evaluate the antimicrobial and biofilm anti-adhesion activities of poly(vinyl alcohol)-coated silver nanoparticles (AgNPs-PVA) and farnesol against Enterococcus faecalis, Candida albicans or Pseudomonas aeruginosa. Design: Minimum inhibitory concentration (MIC) and minimum microbicidal concentration (MMC) of the solutions, as well as the effect on the biofilm biomass were evaluated. The biofilm anti-adhesion activity was evaluated using bovine root dentine treated with the solutions after 3 min of contact and analyzed by scanning electron microscopy (SEM) and by colony-forming units per milliliter (CFU mL−1) counting. Data were analyzed using ANOVA and Tukey's, the paired Student's t-test or Kruskal-Wallis and Dunn's tests (α = 0.05). Results: The MIC and MMC values (MIC/MMC) of the AgNPs-PVA and farnesol against E. faecalis were 42.5/50 μM and 0.85/1.0%, respectively. For C. albicans, the values were 27.5/37.5 μM and 1.75/2.5%; and for P. aeruginosa, 32.5/32.5 μM and 2.5/2.75%, respectively. Both solutions showed reduced biofilm biomass (p < 0.05). SEM analysis showed that dentine blocks treated with both solutions had lower biofilm formation than the control (saline), except for C. albicans. In the CFU mL−1 counting, biofilm cells were viable in the all groups in comparison with control (p > 0.05). Conclusions: AgNPs-PVA and farnesol showed antimicrobial and biofilm anti-adhesion activities, as well as potential for use as coadjuvant in endodontic treatment, and may be an option as auxiliary procedure for root canal disinfection or to inhibit biofilm formation. |
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Antimicrobial and biofilm anti-adhesion activities of silver nanoparticles and farnesol against endodontic microorganisms for possible application in root canal treatmentBiofilmEndodonticsFarnesolSilver nanoparticlesObjective: This study aimed to evaluate the antimicrobial and biofilm anti-adhesion activities of poly(vinyl alcohol)-coated silver nanoparticles (AgNPs-PVA) and farnesol against Enterococcus faecalis, Candida albicans or Pseudomonas aeruginosa. Design: Minimum inhibitory concentration (MIC) and minimum microbicidal concentration (MMC) of the solutions, as well as the effect on the biofilm biomass were evaluated. The biofilm anti-adhesion activity was evaluated using bovine root dentine treated with the solutions after 3 min of contact and analyzed by scanning electron microscopy (SEM) and by colony-forming units per milliliter (CFU mL−1) counting. Data were analyzed using ANOVA and Tukey's, the paired Student's t-test or Kruskal-Wallis and Dunn's tests (α = 0.05). Results: The MIC and MMC values (MIC/MMC) of the AgNPs-PVA and farnesol against E. faecalis were 42.5/50 μM and 0.85/1.0%, respectively. For C. albicans, the values were 27.5/37.5 μM and 1.75/2.5%; and for P. aeruginosa, 32.5/32.5 μM and 2.5/2.75%, respectively. Both solutions showed reduced biofilm biomass (p < 0.05). SEM analysis showed that dentine blocks treated with both solutions had lower biofilm formation than the control (saline), except for C. albicans. In the CFU mL−1 counting, biofilm cells were viable in the all groups in comparison with control (p > 0.05). Conclusions: AgNPs-PVA and farnesol showed antimicrobial and biofilm anti-adhesion activities, as well as potential for use as coadjuvant in endodontic treatment, and may be an option as auxiliary procedure for root canal disinfection or to inhibit biofilm formation.Department of Restorative Dentistry São Paulo State University (UNESP) School of DentistryInstitute of Physics University of Sao Paulo (USP)Department of Restorative Dentistry São Paulo State University (UNESP) School of DentistryUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Chávez-Andrade, Gisselle Moraima [UNESP]Tanomaru-Filho, Mário [UNESP]Basso Bernardi, Maria Inêsde Toledo Leonardo, Renato [UNESP]Faria, Gisele [UNESP]Guerreiro-Tanomaru, Juliane Maria [UNESP]2019-10-06T17:15:45Z2019-10-06T17:15:45Z2019-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.archoralbio.2019.104481Archives of Oral Biology, v. 107.1879-15060003-9969http://hdl.handle.net/11449/19051510.1016/j.archoralbio.2019.1044812-s2.0-85069558562Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArchives of Oral Biologyinfo:eu-repo/semantics/openAccess2021-10-23T19:23:36Zoai:repositorio.unesp.br:11449/190515Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T19:23:36Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Antimicrobial and biofilm anti-adhesion activities of silver nanoparticles and farnesol against endodontic microorganisms for possible application in root canal treatment |
title |
Antimicrobial and biofilm anti-adhesion activities of silver nanoparticles and farnesol against endodontic microorganisms for possible application in root canal treatment |
spellingShingle |
Antimicrobial and biofilm anti-adhesion activities of silver nanoparticles and farnesol against endodontic microorganisms for possible application in root canal treatment Chávez-Andrade, Gisselle Moraima [UNESP] Biofilm Endodontics Farnesol Silver nanoparticles |
title_short |
Antimicrobial and biofilm anti-adhesion activities of silver nanoparticles and farnesol against endodontic microorganisms for possible application in root canal treatment |
title_full |
Antimicrobial and biofilm anti-adhesion activities of silver nanoparticles and farnesol against endodontic microorganisms for possible application in root canal treatment |
title_fullStr |
Antimicrobial and biofilm anti-adhesion activities of silver nanoparticles and farnesol against endodontic microorganisms for possible application in root canal treatment |
title_full_unstemmed |
Antimicrobial and biofilm anti-adhesion activities of silver nanoparticles and farnesol against endodontic microorganisms for possible application in root canal treatment |
title_sort |
Antimicrobial and biofilm anti-adhesion activities of silver nanoparticles and farnesol against endodontic microorganisms for possible application in root canal treatment |
author |
Chávez-Andrade, Gisselle Moraima [UNESP] |
author_facet |
Chávez-Andrade, Gisselle Moraima [UNESP] Tanomaru-Filho, Mário [UNESP] Basso Bernardi, Maria Inês de Toledo Leonardo, Renato [UNESP] Faria, Gisele [UNESP] Guerreiro-Tanomaru, Juliane Maria [UNESP] |
author_role |
author |
author2 |
Tanomaru-Filho, Mário [UNESP] Basso Bernardi, Maria Inês de Toledo Leonardo, Renato [UNESP] Faria, Gisele [UNESP] Guerreiro-Tanomaru, Juliane Maria [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Chávez-Andrade, Gisselle Moraima [UNESP] Tanomaru-Filho, Mário [UNESP] Basso Bernardi, Maria Inês de Toledo Leonardo, Renato [UNESP] Faria, Gisele [UNESP] Guerreiro-Tanomaru, Juliane Maria [UNESP] |
dc.subject.por.fl_str_mv |
Biofilm Endodontics Farnesol Silver nanoparticles |
topic |
Biofilm Endodontics Farnesol Silver nanoparticles |
description |
Objective: This study aimed to evaluate the antimicrobial and biofilm anti-adhesion activities of poly(vinyl alcohol)-coated silver nanoparticles (AgNPs-PVA) and farnesol against Enterococcus faecalis, Candida albicans or Pseudomonas aeruginosa. Design: Minimum inhibitory concentration (MIC) and minimum microbicidal concentration (MMC) of the solutions, as well as the effect on the biofilm biomass were evaluated. The biofilm anti-adhesion activity was evaluated using bovine root dentine treated with the solutions after 3 min of contact and analyzed by scanning electron microscopy (SEM) and by colony-forming units per milliliter (CFU mL−1) counting. Data were analyzed using ANOVA and Tukey's, the paired Student's t-test or Kruskal-Wallis and Dunn's tests (α = 0.05). Results: The MIC and MMC values (MIC/MMC) of the AgNPs-PVA and farnesol against E. faecalis were 42.5/50 μM and 0.85/1.0%, respectively. For C. albicans, the values were 27.5/37.5 μM and 1.75/2.5%; and for P. aeruginosa, 32.5/32.5 μM and 2.5/2.75%, respectively. Both solutions showed reduced biofilm biomass (p < 0.05). SEM analysis showed that dentine blocks treated with both solutions had lower biofilm formation than the control (saline), except for C. albicans. In the CFU mL−1 counting, biofilm cells were viable in the all groups in comparison with control (p > 0.05). Conclusions: AgNPs-PVA and farnesol showed antimicrobial and biofilm anti-adhesion activities, as well as potential for use as coadjuvant in endodontic treatment, and may be an option as auxiliary procedure for root canal disinfection or to inhibit biofilm formation. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T17:15:45Z 2019-10-06T17:15:45Z 2019-11-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.archoralbio.2019.104481 Archives of Oral Biology, v. 107. 1879-1506 0003-9969 http://hdl.handle.net/11449/190515 10.1016/j.archoralbio.2019.104481 2-s2.0-85069558562 |
url |
http://dx.doi.org/10.1016/j.archoralbio.2019.104481 http://hdl.handle.net/11449/190515 |
identifier_str_mv |
Archives of Oral Biology, v. 107. 1879-1506 0003-9969 10.1016/j.archoralbio.2019.104481 2-s2.0-85069558562 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Archives of Oral Biology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965314912354304 |