Evaluation of the genotoxicity/mutagenicity and antigenotoxicity/antimutagenicity induced by propolis and Baccharis dracunculifolia, by in vitro study with HTC cells

Detalhes bibliográficos
Autor(a) principal: Roberto, Matheus Mantuanelli [UNESP]
Data de Publicação: 2016
Outros Autores: Matsumoto, Sílvia Tamie, Jamal, Cláudia Masrouah, Malaspina, Osmar [UNESP], Marin-Morales, Maria Aparecida [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.tiv.2016.02.005
http://hdl.handle.net/11449/177840
Resumo: The ethanolic extract of propolis, especially the Brazilian green type, is widely and mainly used for therapeutic purposes despite the lack of knowledge about its effects and its cellular mode of action. This type of propolis, derived from Baccharis dracunculifolia (alecrim-do-campo), has been extensively commercialized and the consumers use it to enhance health. This work aimed to assess the genotoxic/mutagenic and antigenotoxic/antimutagenic potentials of the ethanolic extracts of Brazilian green propolis and of B. dracunculifolia, on mammalian cells. It was not observed genotoxic and mutagenic effects by both extracts. After evaluate the exposure of the cells to each extract with a recognized mutagen, simultaneously, the results showed a significant reduction on DNA damage. The experiment carried out with a pre-incubation period was more effective than without incubation test, showing that the tested extracts were able to inactivate the mutagen before it could react with the DNA.
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spelling Evaluation of the genotoxicity/mutagenicity and antigenotoxicity/antimutagenicity induced by propolis and Baccharis dracunculifolia, by in vitro study with HTC cellsBrazilian green propolisComet assayCytokinesis-blockage micronucleus testEthanolic extractMutagen inactivationThe ethanolic extract of propolis, especially the Brazilian green type, is widely and mainly used for therapeutic purposes despite the lack of knowledge about its effects and its cellular mode of action. This type of propolis, derived from Baccharis dracunculifolia (alecrim-do-campo), has been extensively commercialized and the consumers use it to enhance health. This work aimed to assess the genotoxic/mutagenic and antigenotoxic/antimutagenic potentials of the ethanolic extracts of Brazilian green propolis and of B. dracunculifolia, on mammalian cells. It was not observed genotoxic and mutagenic effects by both extracts. After evaluate the exposure of the cells to each extract with a recognized mutagen, simultaneously, the results showed a significant reduction on DNA damage. The experiment carried out with a pre-incubation period was more effective than without incubation test, showing that the tested extracts were able to inactivate the mutagen before it could react with the DNA.Departamento de Biologia Instituto de Biociências UNESP - Univ. Estadual Paulista, Avenida 24-A, 1515, CP199Departamento de Ciências Biológicas Centro de Ciências Humanas e Naturais UFES - Universidade Federal do Espírito Santo, Avenida Fernando Ferrari, 514Departamento de Ciências Farmacêuticas Centro de Ciências da Saúde UFES - Universidade Federal do Espírito Santo, Avenida Marechal Campos, 1468Departamento de Biologia Instituto de Biociências UNESP - Univ. Estadual Paulista, Avenida 24-A, 1515, CP199Universidade Estadual Paulista (Unesp)Universidade Federal do Espírito Santo (UFES)Roberto, Matheus Mantuanelli [UNESP]Matsumoto, Sílvia TamieJamal, Cláudia MasrouahMalaspina, Osmar [UNESP]Marin-Morales, Maria Aparecida [UNESP]2018-12-11T17:27:23Z2018-12-11T17:27:23Z2016-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article9-15application/pdfhttp://dx.doi.org/10.1016/j.tiv.2016.02.005Toxicology in Vitro, v. 33, p. 9-15.1879-31770887-2333http://hdl.handle.net/11449/17784010.1016/j.tiv.2016.02.0052-s2.0-849592159222-s2.0-84959215922.pdf75385560855058190000-0002-1650-257XScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengToxicology in Vitro0,931info:eu-repo/semantics/openAccess2024-01-11T06:31:21Zoai:repositorio.unesp.br:11449/177840Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-11T06:31:21Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Evaluation of the genotoxicity/mutagenicity and antigenotoxicity/antimutagenicity induced by propolis and Baccharis dracunculifolia, by in vitro study with HTC cells
title Evaluation of the genotoxicity/mutagenicity and antigenotoxicity/antimutagenicity induced by propolis and Baccharis dracunculifolia, by in vitro study with HTC cells
spellingShingle Evaluation of the genotoxicity/mutagenicity and antigenotoxicity/antimutagenicity induced by propolis and Baccharis dracunculifolia, by in vitro study with HTC cells
Roberto, Matheus Mantuanelli [UNESP]
Brazilian green propolis
Comet assay
Cytokinesis-blockage micronucleus test
Ethanolic extract
Mutagen inactivation
title_short Evaluation of the genotoxicity/mutagenicity and antigenotoxicity/antimutagenicity induced by propolis and Baccharis dracunculifolia, by in vitro study with HTC cells
title_full Evaluation of the genotoxicity/mutagenicity and antigenotoxicity/antimutagenicity induced by propolis and Baccharis dracunculifolia, by in vitro study with HTC cells
title_fullStr Evaluation of the genotoxicity/mutagenicity and antigenotoxicity/antimutagenicity induced by propolis and Baccharis dracunculifolia, by in vitro study with HTC cells
title_full_unstemmed Evaluation of the genotoxicity/mutagenicity and antigenotoxicity/antimutagenicity induced by propolis and Baccharis dracunculifolia, by in vitro study with HTC cells
title_sort Evaluation of the genotoxicity/mutagenicity and antigenotoxicity/antimutagenicity induced by propolis and Baccharis dracunculifolia, by in vitro study with HTC cells
author Roberto, Matheus Mantuanelli [UNESP]
author_facet Roberto, Matheus Mantuanelli [UNESP]
Matsumoto, Sílvia Tamie
Jamal, Cláudia Masrouah
Malaspina, Osmar [UNESP]
Marin-Morales, Maria Aparecida [UNESP]
author_role author
author2 Matsumoto, Sílvia Tamie
Jamal, Cláudia Masrouah
Malaspina, Osmar [UNESP]
Marin-Morales, Maria Aparecida [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade Federal do Espírito Santo (UFES)
dc.contributor.author.fl_str_mv Roberto, Matheus Mantuanelli [UNESP]
Matsumoto, Sílvia Tamie
Jamal, Cláudia Masrouah
Malaspina, Osmar [UNESP]
Marin-Morales, Maria Aparecida [UNESP]
dc.subject.por.fl_str_mv Brazilian green propolis
Comet assay
Cytokinesis-blockage micronucleus test
Ethanolic extract
Mutagen inactivation
topic Brazilian green propolis
Comet assay
Cytokinesis-blockage micronucleus test
Ethanolic extract
Mutagen inactivation
description The ethanolic extract of propolis, especially the Brazilian green type, is widely and mainly used for therapeutic purposes despite the lack of knowledge about its effects and its cellular mode of action. This type of propolis, derived from Baccharis dracunculifolia (alecrim-do-campo), has been extensively commercialized and the consumers use it to enhance health. This work aimed to assess the genotoxic/mutagenic and antigenotoxic/antimutagenic potentials of the ethanolic extracts of Brazilian green propolis and of B. dracunculifolia, on mammalian cells. It was not observed genotoxic and mutagenic effects by both extracts. After evaluate the exposure of the cells to each extract with a recognized mutagen, simultaneously, the results showed a significant reduction on DNA damage. The experiment carried out with a pre-incubation period was more effective than without incubation test, showing that the tested extracts were able to inactivate the mutagen before it could react with the DNA.
publishDate 2016
dc.date.none.fl_str_mv 2016-06-01
2018-12-11T17:27:23Z
2018-12-11T17:27:23Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.tiv.2016.02.005
Toxicology in Vitro, v. 33, p. 9-15.
1879-3177
0887-2333
http://hdl.handle.net/11449/177840
10.1016/j.tiv.2016.02.005
2-s2.0-84959215922
2-s2.0-84959215922.pdf
7538556085505819
0000-0002-1650-257X
url http://dx.doi.org/10.1016/j.tiv.2016.02.005
http://hdl.handle.net/11449/177840
identifier_str_mv Toxicology in Vitro, v. 33, p. 9-15.
1879-3177
0887-2333
10.1016/j.tiv.2016.02.005
2-s2.0-84959215922
2-s2.0-84959215922.pdf
7538556085505819
0000-0002-1650-257X
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Toxicology in Vitro
0,931
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 9-15
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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