Comparison of conventional and contemporary root canal disinfection protocols against bacteria, lipoteichoic acid (LTA), and lipopolysaccharide (LPS)
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1038/s41598-022-26855-y http://hdl.handle.net/11449/246697 |
Resumo: | This study devised a dual-species biofilm model to investigate bacteria, lipoteichoic acid (LTA), and lipopolysaccharide (LPS) simultaneously, and compared the efficacy of conventional and contemporary disinfection protocols. Seventy single-rooted mandibular premolars were included. Fourteen teeth were negative control, and 56 teeth were infected with 3-week-old E. faecalis and E. coli GFP biofilm. Fourteen/56 teeth were positive control, with seven teeth processed for CLSM analysis and seven teeth sampled with paper points (PPs) and cryogenically ground for bacterial, LTA, and LPS analyses. Forty-two teeth were randomly divided into three groups: GWS (GentleWave system) + MIT (minimally invasive technique), PUI (passive ultrasonic irrigation) + CIT (conventional instrumentation technique), and XP-EF (XP-endo Finisher) + CIT (All, n = 14). Samples were collected before (s1) and after disinfection (s2) with PPs and after cryogenically ground (s3). CFUs were counted, and LTA and LPS were quantified with LTA-ELISA and LAL assay, respectively. XP-EF was as effective as PUI (p > 0.05). GWS + MIT was the most effective disinfection protocol against bacteria, LTA, and LPS (p < 0.05). In conclusion, PUI, XP-EF, and GWS were highly effective against bacteria, LTA, and LPS, with GWS being the most effective. |
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Comparison of conventional and contemporary root canal disinfection protocols against bacteria, lipoteichoic acid (LTA), and lipopolysaccharide (LPS)This study devised a dual-species biofilm model to investigate bacteria, lipoteichoic acid (LTA), and lipopolysaccharide (LPS) simultaneously, and compared the efficacy of conventional and contemporary disinfection protocols. Seventy single-rooted mandibular premolars were included. Fourteen teeth were negative control, and 56 teeth were infected with 3-week-old E. faecalis and E. coli GFP biofilm. Fourteen/56 teeth were positive control, with seven teeth processed for CLSM analysis and seven teeth sampled with paper points (PPs) and cryogenically ground for bacterial, LTA, and LPS analyses. Forty-two teeth were randomly divided into three groups: GWS (GentleWave system) + MIT (minimally invasive technique), PUI (passive ultrasonic irrigation) + CIT (conventional instrumentation technique), and XP-EF (XP-endo Finisher) + CIT (All, n = 14). Samples were collected before (s1) and after disinfection (s2) with PPs and after cryogenically ground (s3). CFUs were counted, and LTA and LPS were quantified with LTA-ELISA and LAL assay, respectively. XP-EF was as effective as PUI (p > 0.05). GWS + MIT was the most effective disinfection protocol against bacteria, LTA, and LPS (p < 0.05). In conclusion, PUI, XP-EF, and GWS were highly effective against bacteria, LTA, and LPS, with GWS being the most effective.Department of Advanced Oral Sciences and Therapeutics School of Dentistry University of MarylandDepartment of Restorative Dental Science College of Dentistry Imam Abdulrahman Bin Faisal UniversityPrince Abdulrahman Advanced Dental InstituteCollege of Dentistry University of KentuckyDepartment of General Dentistry School of Dentistry University of MarylandEndodontic Division Department of Restorative Dentistry Institute of Science and Technology São Paulo State University (Unesp)Department of Social and Pediatric Dentistry Institute of Science and Technology Institute of Science and Technology São Paulo State University (Unesp)Endodontic Division Department of Restorative Dentistry Institute of Science and Technology São Paulo State University (Unesp)Department of Social and Pediatric Dentistry Institute of Science and Technology Institute of Science and Technology São Paulo State University (Unesp)University of MarylandImam Abdulrahman Bin Faisal UniversityPrince Abdulrahman Advanced Dental InstituteUniversity of KentuckyUniversidade Estadual Paulista (UNESP)Alquria, Theeb A.Alfirdous, Rayyan A.Gupta, SwatiSantamaria, Mauro P.Santamaria, Ingrid F.Gomes, Ana P. M. [UNESP]Tiradentes, Naiara [UNESP]Silva, Eduardo G. [UNESP]Martinho, Frederico C.2023-07-29T12:48:02Z2023-07-29T12:48:02Z2023-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1038/s41598-022-26855-yScientific Reports, v. 13, n. 1, 2023.2045-2322http://hdl.handle.net/11449/24669710.1038/s41598-022-26855-y2-s2.0-85146697904Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengScientific Reportsinfo:eu-repo/semantics/openAccess2023-07-29T12:48:02Zoai:repositorio.unesp.br:11449/246697Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-07-29T12:48:02Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Comparison of conventional and contemporary root canal disinfection protocols against bacteria, lipoteichoic acid (LTA), and lipopolysaccharide (LPS) |
title |
Comparison of conventional and contemporary root canal disinfection protocols against bacteria, lipoteichoic acid (LTA), and lipopolysaccharide (LPS) |
spellingShingle |
Comparison of conventional and contemporary root canal disinfection protocols against bacteria, lipoteichoic acid (LTA), and lipopolysaccharide (LPS) Alquria, Theeb A. |
title_short |
Comparison of conventional and contemporary root canal disinfection protocols against bacteria, lipoteichoic acid (LTA), and lipopolysaccharide (LPS) |
title_full |
Comparison of conventional and contemporary root canal disinfection protocols against bacteria, lipoteichoic acid (LTA), and lipopolysaccharide (LPS) |
title_fullStr |
Comparison of conventional and contemporary root canal disinfection protocols against bacteria, lipoteichoic acid (LTA), and lipopolysaccharide (LPS) |
title_full_unstemmed |
Comparison of conventional and contemporary root canal disinfection protocols against bacteria, lipoteichoic acid (LTA), and lipopolysaccharide (LPS) |
title_sort |
Comparison of conventional and contemporary root canal disinfection protocols against bacteria, lipoteichoic acid (LTA), and lipopolysaccharide (LPS) |
author |
Alquria, Theeb A. |
author_facet |
Alquria, Theeb A. Alfirdous, Rayyan A. Gupta, Swati Santamaria, Mauro P. Santamaria, Ingrid F. Gomes, Ana P. M. [UNESP] Tiradentes, Naiara [UNESP] Silva, Eduardo G. [UNESP] Martinho, Frederico C. |
author_role |
author |
author2 |
Alfirdous, Rayyan A. Gupta, Swati Santamaria, Mauro P. Santamaria, Ingrid F. Gomes, Ana P. M. [UNESP] Tiradentes, Naiara [UNESP] Silva, Eduardo G. [UNESP] Martinho, Frederico C. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
University of Maryland Imam Abdulrahman Bin Faisal University Prince Abdulrahman Advanced Dental Institute University of Kentucky Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Alquria, Theeb A. Alfirdous, Rayyan A. Gupta, Swati Santamaria, Mauro P. Santamaria, Ingrid F. Gomes, Ana P. M. [UNESP] Tiradentes, Naiara [UNESP] Silva, Eduardo G. [UNESP] Martinho, Frederico C. |
description |
This study devised a dual-species biofilm model to investigate bacteria, lipoteichoic acid (LTA), and lipopolysaccharide (LPS) simultaneously, and compared the efficacy of conventional and contemporary disinfection protocols. Seventy single-rooted mandibular premolars were included. Fourteen teeth were negative control, and 56 teeth were infected with 3-week-old E. faecalis and E. coli GFP biofilm. Fourteen/56 teeth were positive control, with seven teeth processed for CLSM analysis and seven teeth sampled with paper points (PPs) and cryogenically ground for bacterial, LTA, and LPS analyses. Forty-two teeth were randomly divided into three groups: GWS (GentleWave system) + MIT (minimally invasive technique), PUI (passive ultrasonic irrigation) + CIT (conventional instrumentation technique), and XP-EF (XP-endo Finisher) + CIT (All, n = 14). Samples were collected before (s1) and after disinfection (s2) with PPs and after cryogenically ground (s3). CFUs were counted, and LTA and LPS were quantified with LTA-ELISA and LAL assay, respectively. XP-EF was as effective as PUI (p > 0.05). GWS + MIT was the most effective disinfection protocol against bacteria, LTA, and LPS (p < 0.05). In conclusion, PUI, XP-EF, and GWS were highly effective against bacteria, LTA, and LPS, with GWS being the most effective. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T12:48:02Z 2023-07-29T12:48:02Z 2023-12-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1038/s41598-022-26855-y Scientific Reports, v. 13, n. 1, 2023. 2045-2322 http://hdl.handle.net/11449/246697 10.1038/s41598-022-26855-y 2-s2.0-85146697904 |
url |
http://dx.doi.org/10.1038/s41598-022-26855-y http://hdl.handle.net/11449/246697 |
identifier_str_mv |
Scientific Reports, v. 13, n. 1, 2023. 2045-2322 10.1038/s41598-022-26855-y 2-s2.0-85146697904 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Scientific Reports |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799964859216953344 |