A comparative in silico linear B-cell epitope prediction and characterization for South American and African Trypanosoma vivax strains
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2018 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UNESP |
| Texto Completo: | http://dx.doi.org/10.1016/j.ygeno.2018.02.017 http://hdl.handle.net/11449/179635 |
Resumo: | Trypanosoma vivax is a parasite widespread across Africa and South America. Immunological methods using recombinant antigens have been developed aiming at specific and sensitive detection of infections caused by T. vivax. Here, we sequenced for the first time the transcriptome of a virulent T. vivax strain (Lins), isolated from an outbreak of severe disease in South America (Brazil) and performed a computational integrated analysis of genome, transcriptome and in silico predictions to identify and characterize putative linear B-cell epitopes from African and South American T. vivax. A total of 2278, 3936 and 4062 linear B-cell epitopes were respectively characterized for the transcriptomes of T. vivax LIEM-176 (Venezuela), T. vivax IL1392 (Nigeria) and T. vivax Lins (Brazil) and 4684 for the genome of T. vivax Y486 (Nigeria). The results presented are a valuable theoretical source that may pave the way for highly sensitive and specific diagnostic tools. |
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A comparative in silico linear B-cell epitope prediction and characterization for South American and African Trypanosoma vivax strainsBioinformaticsBloodstream formsLinear B-cell epitopesTranscriptomicsTrypanosoma vivaxTrypanosoma vivax is a parasite widespread across Africa and South America. Immunological methods using recombinant antigens have been developed aiming at specific and sensitive detection of infections caused by T. vivax. Here, we sequenced for the first time the transcriptome of a virulent T. vivax strain (Lins), isolated from an outbreak of severe disease in South America (Brazil) and performed a computational integrated analysis of genome, transcriptome and in silico predictions to identify and characterize putative linear B-cell epitopes from African and South American T. vivax. A total of 2278, 3936 and 4062 linear B-cell epitopes were respectively characterized for the transcriptomes of T. vivax LIEM-176 (Venezuela), T. vivax IL1392 (Nigeria) and T. vivax Lins (Brazil) and 4684 for the genome of T. vivax Y486 (Nigeria). The results presented are a valuable theoretical source that may pave the way for highly sensitive and specific diagnostic tools.Laboratório Nacional de Computação Científica (LNCC), Av. Getúlio Vargas, 333Grupo Hermes Pardini Setor de Pesquisa e DesenvolvimentoDepartamento de Parasitologia Instituto de Ciências Biomédicas Universidade de São PauloTrypanosomatids Infectious Processes Laboratory Department of Infection and Epidemiology Institut Pasteur, 25 rue du Dr RouxDepartamento Clínica Cirurgia e Reprodução Animal Faculdade de Odontologia e Curso de Medicina Veterinária Universidade Estadual Paulista – UNESPLaboratório de Immnoparasitologia Faculdade de Ciências Agrárias e Veterinárias (FCAV) Universidade Estadual Paulista (UNESP), Campus JaboticabalDepartamento Clínica Cirurgia e Reprodução Animal Faculdade de Odontologia e Curso de Medicina Veterinária Universidade Estadual Paulista – UNESPLaboratório de Immnoparasitologia Faculdade de Ciências Agrárias e Veterinárias (FCAV) Universidade Estadual Paulista (UNESP), Campus JaboticabalLaboratório Nacional de Computação Científica (LNCC)Setor de Pesquisa e DesenvolvimentoUniversidade de São Paulo (USP)Institut PasteurUniversidade Estadual Paulista (Unesp)Guedes, Rafael Lucas MunizRodrigues, Carla Monadeli FilgueiraCoatnoan, NicolasCosson, AlainCadioli, Fabiano Antonio [UNESP]Garcia, Herakles AntonioGerber, Alexandra LehmkuhlMachado, Rosangela Zacarias [UNESP]Minoprio, Paola Marcella CamargoTeixeira, Marta Maria Geraldesde Vasconcelos, Ana Tereza Ribeiro2018-12-11T17:36:08Z2018-12-11T17:36:08Z2018-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1016/j.ygeno.2018.02.017Genomics.1089-86460888-7543http://hdl.handle.net/11449/17963510.1016/j.ygeno.2018.02.0172-s2.0-850429090092-s2.0-85042909009.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenomics1,6881,688info:eu-repo/semantics/openAccess2023-11-19T06:08:03Zoai:repositorio.unesp.br:11449/179635Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-19T06:08:03Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
| dc.title.none.fl_str_mv |
A comparative in silico linear B-cell epitope prediction and characterization for South American and African Trypanosoma vivax strains |
| title |
A comparative in silico linear B-cell epitope prediction and characterization for South American and African Trypanosoma vivax strains |
| spellingShingle |
A comparative in silico linear B-cell epitope prediction and characterization for South American and African Trypanosoma vivax strains Guedes, Rafael Lucas Muniz Bioinformatics Bloodstream forms Linear B-cell epitopes Transcriptomics Trypanosoma vivax |
| title_short |
A comparative in silico linear B-cell epitope prediction and characterization for South American and African Trypanosoma vivax strains |
| title_full |
A comparative in silico linear B-cell epitope prediction and characterization for South American and African Trypanosoma vivax strains |
| title_fullStr |
A comparative in silico linear B-cell epitope prediction and characterization for South American and African Trypanosoma vivax strains |
| title_full_unstemmed |
A comparative in silico linear B-cell epitope prediction and characterization for South American and African Trypanosoma vivax strains |
| title_sort |
A comparative in silico linear B-cell epitope prediction and characterization for South American and African Trypanosoma vivax strains |
| author |
Guedes, Rafael Lucas Muniz |
| author_facet |
Guedes, Rafael Lucas Muniz Rodrigues, Carla Monadeli Filgueira Coatnoan, Nicolas Cosson, Alain Cadioli, Fabiano Antonio [UNESP] Garcia, Herakles Antonio Gerber, Alexandra Lehmkuhl Machado, Rosangela Zacarias [UNESP] Minoprio, Paola Marcella Camargo Teixeira, Marta Maria Geraldes de Vasconcelos, Ana Tereza Ribeiro |
| author_role |
author |
| author2 |
Rodrigues, Carla Monadeli Filgueira Coatnoan, Nicolas Cosson, Alain Cadioli, Fabiano Antonio [UNESP] Garcia, Herakles Antonio Gerber, Alexandra Lehmkuhl Machado, Rosangela Zacarias [UNESP] Minoprio, Paola Marcella Camargo Teixeira, Marta Maria Geraldes de Vasconcelos, Ana Tereza Ribeiro |
| author2_role |
author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Laboratório Nacional de Computação Científica (LNCC) Setor de Pesquisa e Desenvolvimento Universidade de São Paulo (USP) Institut Pasteur Universidade Estadual Paulista (Unesp) |
| dc.contributor.author.fl_str_mv |
Guedes, Rafael Lucas Muniz Rodrigues, Carla Monadeli Filgueira Coatnoan, Nicolas Cosson, Alain Cadioli, Fabiano Antonio [UNESP] Garcia, Herakles Antonio Gerber, Alexandra Lehmkuhl Machado, Rosangela Zacarias [UNESP] Minoprio, Paola Marcella Camargo Teixeira, Marta Maria Geraldes de Vasconcelos, Ana Tereza Ribeiro |
| dc.subject.por.fl_str_mv |
Bioinformatics Bloodstream forms Linear B-cell epitopes Transcriptomics Trypanosoma vivax |
| topic |
Bioinformatics Bloodstream forms Linear B-cell epitopes Transcriptomics Trypanosoma vivax |
| description |
Trypanosoma vivax is a parasite widespread across Africa and South America. Immunological methods using recombinant antigens have been developed aiming at specific and sensitive detection of infections caused by T. vivax. Here, we sequenced for the first time the transcriptome of a virulent T. vivax strain (Lins), isolated from an outbreak of severe disease in South America (Brazil) and performed a computational integrated analysis of genome, transcriptome and in silico predictions to identify and characterize putative linear B-cell epitopes from African and South American T. vivax. A total of 2278, 3936 and 4062 linear B-cell epitopes were respectively characterized for the transcriptomes of T. vivax LIEM-176 (Venezuela), T. vivax IL1392 (Nigeria) and T. vivax Lins (Brazil) and 4684 for the genome of T. vivax Y486 (Nigeria). The results presented are a valuable theoretical source that may pave the way for highly sensitive and specific diagnostic tools. |
| publishDate |
2018 |
| dc.date.none.fl_str_mv |
2018-12-11T17:36:08Z 2018-12-11T17:36:08Z 2018-01-01 |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.ygeno.2018.02.017 Genomics. 1089-8646 0888-7543 http://hdl.handle.net/11449/179635 10.1016/j.ygeno.2018.02.017 2-s2.0-85042909009 2-s2.0-85042909009.pdf |
| url |
http://dx.doi.org/10.1016/j.ygeno.2018.02.017 http://hdl.handle.net/11449/179635 |
| identifier_str_mv |
Genomics. 1089-8646 0888-7543 10.1016/j.ygeno.2018.02.017 2-s2.0-85042909009 2-s2.0-85042909009.pdf |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Genomics 1,688 1,688 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
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Universidade Estadual Paulista (UNESP) |
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UNESP |
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UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
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