The genotoxic effects of fruit extract of Crataegus oxyacantha (hawthorn) in mice
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1080/15287394.2018.1503982 http://hdl.handle.net/11449/188228 |
Resumo: | Crataegus oxyacantha L. (Rosaceae) is a medicinal plant with a long history of use in European, Chinese, and American. The majority of pharmacological activities associated with fruit extracts of C. oxyacantha L. are related to cardio-stimulant properties utilized in the treatment of atherosclerosis, hypertension with myocardic insufficiency, angina pectoris, cardiac rhythm alterations, and heart failure. Some other therapeutic uses for renal calculi, dyspnea, as well as a diuretic, sedative, and anxiolytic were also reported. Due to the beneficial potential of C. oxyacantha fruits extract but evidence in vitro of genetic toxicity, the aim of the present study was to examine the genotoxic potential of plant extract in vivo in mice. The extract was administered orally, daily by gavage at doses of 50, 100, and 200 mg/kg body weight for seven days. Data demonstrated that C. oxyacantha extract did not markedly induce DNA damage in leukocytes and bone marrow cells by the comet assay; however, the extract produced a significant rise in micronucleated polychromatic erythrocytes (PCE) at all tested doses in a non-dose dependent manner as evidenced by the micronucleus test. The PCE/normochromatic erythrocytes (NCE) ratio indicated no significant cytotoxicity. Under our experimental conditions, C. oxyacantha fruits extract exhibited weak clastogenic and/or aneugenic effects in bone marrow cells of male mice, confirming our previous in vitro findings that this plant extract induced genotoxicity suggesting that prolonged or high dose use needs to be undertaken with caution. |
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The genotoxic effects of fruit extract of Crataegus oxyacantha (hawthorn) in miceclastogenic effectscomet assaymicronucleus testRosaceaeCrataegus oxyacantha L. (Rosaceae) is a medicinal plant with a long history of use in European, Chinese, and American. The majority of pharmacological activities associated with fruit extracts of C. oxyacantha L. are related to cardio-stimulant properties utilized in the treatment of atherosclerosis, hypertension with myocardic insufficiency, angina pectoris, cardiac rhythm alterations, and heart failure. Some other therapeutic uses for renal calculi, dyspnea, as well as a diuretic, sedative, and anxiolytic were also reported. Due to the beneficial potential of C. oxyacantha fruits extract but evidence in vitro of genetic toxicity, the aim of the present study was to examine the genotoxic potential of plant extract in vivo in mice. The extract was administered orally, daily by gavage at doses of 50, 100, and 200 mg/kg body weight for seven days. Data demonstrated that C. oxyacantha extract did not markedly induce DNA damage in leukocytes and bone marrow cells by the comet assay; however, the extract produced a significant rise in micronucleated polychromatic erythrocytes (PCE) at all tested doses in a non-dose dependent manner as evidenced by the micronucleus test. The PCE/normochromatic erythrocytes (NCE) ratio indicated no significant cytotoxicity. Under our experimental conditions, C. oxyacantha fruits extract exhibited weak clastogenic and/or aneugenic effects in bone marrow cells of male mice, confirming our previous in vitro findings that this plant extract induced genotoxicity suggesting that prolonged or high dose use needs to be undertaken with caution.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Faculdade de Filosofia e Ciências Departamento de Fonoaudiologia São Paulo State University–UNESPQuímicas e Farmacêuticas Departamento de Ciências Exatas e da Terra Universidade Federal de São Paulo–UNIFESP–Instituto de Ciências AmbientaisFaculdade de Ciências Médicas Universidade Estadual de Campinas - UNICAMPCECAV and Department of Genetics and Biotechnology Trás-os-Montes and Alto Douro UniversityFaculdade de Filosofia e Ciências Departamento de Fonoaudiologia São Paulo State University–UNESPCNPq: 446232/2014-9Universidade Estadual Paulista (Unesp)Universidade Federal de São Paulo (UNIFESP)Universidade Estadual de Campinas (UNICAMP)Trás-os-Montes and Alto Douro UniversityYonekubo, Bruna Thiemi [UNESP]Alves, Heloisa de Miranda Cantuaria [UNESP]de Souza Marques, Eduardo [UNESP]Perazzo, Fábio FerreiraRosa, Paulo César PiresGaivão, Isabel O’Neill de MascarenhasMaistro, Edson Luis [UNESP]2019-10-06T16:01:22Z2019-10-06T16:01:22Z2018-10-02info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article974-982http://dx.doi.org/10.1080/15287394.2018.1503982Journal of Toxicology and Environmental Health - Part A: Current Issues, v. 81, n. 19, p. 974-982, 2018.1087-26201528-7394http://hdl.handle.net/11449/18822810.1080/15287394.2018.15039822-s2.0-8505509523647875216130383150000-0003-0757-7876Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Toxicology and Environmental Health - Part A: Current Issuesinfo:eu-repo/semantics/openAccess2021-10-23T19:28:28Zoai:repositorio.unesp.br:11449/188228Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T19:28:28Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
The genotoxic effects of fruit extract of Crataegus oxyacantha (hawthorn) in mice |
title |
The genotoxic effects of fruit extract of Crataegus oxyacantha (hawthorn) in mice |
spellingShingle |
The genotoxic effects of fruit extract of Crataegus oxyacantha (hawthorn) in mice Yonekubo, Bruna Thiemi [UNESP] clastogenic effects comet assay micronucleus test Rosaceae |
title_short |
The genotoxic effects of fruit extract of Crataegus oxyacantha (hawthorn) in mice |
title_full |
The genotoxic effects of fruit extract of Crataegus oxyacantha (hawthorn) in mice |
title_fullStr |
The genotoxic effects of fruit extract of Crataegus oxyacantha (hawthorn) in mice |
title_full_unstemmed |
The genotoxic effects of fruit extract of Crataegus oxyacantha (hawthorn) in mice |
title_sort |
The genotoxic effects of fruit extract of Crataegus oxyacantha (hawthorn) in mice |
author |
Yonekubo, Bruna Thiemi [UNESP] |
author_facet |
Yonekubo, Bruna Thiemi [UNESP] Alves, Heloisa de Miranda Cantuaria [UNESP] de Souza Marques, Eduardo [UNESP] Perazzo, Fábio Ferreira Rosa, Paulo César Pires Gaivão, Isabel O’Neill de Mascarenhas Maistro, Edson Luis [UNESP] |
author_role |
author |
author2 |
Alves, Heloisa de Miranda Cantuaria [UNESP] de Souza Marques, Eduardo [UNESP] Perazzo, Fábio Ferreira Rosa, Paulo César Pires Gaivão, Isabel O’Neill de Mascarenhas Maistro, Edson Luis [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal de São Paulo (UNIFESP) Universidade Estadual de Campinas (UNICAMP) Trás-os-Montes and Alto Douro University |
dc.contributor.author.fl_str_mv |
Yonekubo, Bruna Thiemi [UNESP] Alves, Heloisa de Miranda Cantuaria [UNESP] de Souza Marques, Eduardo [UNESP] Perazzo, Fábio Ferreira Rosa, Paulo César Pires Gaivão, Isabel O’Neill de Mascarenhas Maistro, Edson Luis [UNESP] |
dc.subject.por.fl_str_mv |
clastogenic effects comet assay micronucleus test Rosaceae |
topic |
clastogenic effects comet assay micronucleus test Rosaceae |
description |
Crataegus oxyacantha L. (Rosaceae) is a medicinal plant with a long history of use in European, Chinese, and American. The majority of pharmacological activities associated with fruit extracts of C. oxyacantha L. are related to cardio-stimulant properties utilized in the treatment of atherosclerosis, hypertension with myocardic insufficiency, angina pectoris, cardiac rhythm alterations, and heart failure. Some other therapeutic uses for renal calculi, dyspnea, as well as a diuretic, sedative, and anxiolytic were also reported. Due to the beneficial potential of C. oxyacantha fruits extract but evidence in vitro of genetic toxicity, the aim of the present study was to examine the genotoxic potential of plant extract in vivo in mice. The extract was administered orally, daily by gavage at doses of 50, 100, and 200 mg/kg body weight for seven days. Data demonstrated that C. oxyacantha extract did not markedly induce DNA damage in leukocytes and bone marrow cells by the comet assay; however, the extract produced a significant rise in micronucleated polychromatic erythrocytes (PCE) at all tested doses in a non-dose dependent manner as evidenced by the micronucleus test. The PCE/normochromatic erythrocytes (NCE) ratio indicated no significant cytotoxicity. Under our experimental conditions, C. oxyacantha fruits extract exhibited weak clastogenic and/or aneugenic effects in bone marrow cells of male mice, confirming our previous in vitro findings that this plant extract induced genotoxicity suggesting that prolonged or high dose use needs to be undertaken with caution. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-10-02 2019-10-06T16:01:22Z 2019-10-06T16:01:22Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1080/15287394.2018.1503982 Journal of Toxicology and Environmental Health - Part A: Current Issues, v. 81, n. 19, p. 974-982, 2018. 1087-2620 1528-7394 http://hdl.handle.net/11449/188228 10.1080/15287394.2018.1503982 2-s2.0-85055095236 4787521613038315 0000-0003-0757-7876 |
url |
http://dx.doi.org/10.1080/15287394.2018.1503982 http://hdl.handle.net/11449/188228 |
identifier_str_mv |
Journal of Toxicology and Environmental Health - Part A: Current Issues, v. 81, n. 19, p. 974-982, 2018. 1087-2620 1528-7394 10.1080/15287394.2018.1503982 2-s2.0-85055095236 4787521613038315 0000-0003-0757-7876 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Toxicology and Environmental Health - Part A: Current Issues |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
974-982 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799965419933532160 |