Acidogenicity of dual-species biofilms of bifidobacteria and Streptococcus mutans
Main Author: | |
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Publication Date: | 2017 |
Other Authors: | , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Institucional da UNESP |
Download full: | http://dx.doi.org/10.1007/s00784-016-1958-1 http://hdl.handle.net/11449/176946 |
Summary: | Objective: The aim of this study was to evaluate the acidogenicity of dual-species biofilms of bifidobacteria and Streptococcus mutans. Materials and methods: The following strains were tested: Bifidobacterium dentium DSM20436, Parascardovia denticolens DSM10105, and Scardovia inopinata DSM10107. Streptococcus mutans UA159 and Lactobacillus acidophilus ATCC4356 were used as control. Bifidobacteria were studied planktonically as they were not able to form monospecies biofilm, they were grown in biofilms associated with S. mutans. Endogenous polysaccharide reserves of cultures at log phase were depleted. Standardized suspensions of the microorganisms were incubated in growth media supplemented with 10 mM glucose, lactose, raffinose, glucose, or xylitol. S. mutans biofilms were grown on glass cover slips for 24 h to which bifidobacteria were added. After 24 h, the dual-species biofilms were exposed to the same carbon sources, and after 3 h, the pH of spent culture media and concentrations of organic acids were measured. Statistical analyses were carried out using ANOVA and Tukey’s test (α = 0.05). Results: A higher pH drop was observed when S. mutans was associated with P. denticolens or S. inopinata, in either planktonic or biofilm cultures, than with S. mutans alone. Bifidobacteria showed a higher pH drop in the presence of raffinose than S. mutans or L. acidophilus. Conclusions: Dual-species biofilms of bifidobacteria and S. mutans produced more acid and greater pH drops than biofilms of S. mutans alone. Clinical relevance: New insights on the complex process of caries pathogenicity contribute to the establishment of preventive and therapeutic measures, in particular in specific cases, such as in early childhood caries. |
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Acidogenicity of dual-species biofilms of bifidobacteria and Streptococcus mutansAcidogenicityBifidobacteriaBiofilmsDental cariesObjective: The aim of this study was to evaluate the acidogenicity of dual-species biofilms of bifidobacteria and Streptococcus mutans. Materials and methods: The following strains were tested: Bifidobacterium dentium DSM20436, Parascardovia denticolens DSM10105, and Scardovia inopinata DSM10107. Streptococcus mutans UA159 and Lactobacillus acidophilus ATCC4356 were used as control. Bifidobacteria were studied planktonically as they were not able to form monospecies biofilm, they were grown in biofilms associated with S. mutans. Endogenous polysaccharide reserves of cultures at log phase were depleted. Standardized suspensions of the microorganisms were incubated in growth media supplemented with 10 mM glucose, lactose, raffinose, glucose, or xylitol. S. mutans biofilms were grown on glass cover slips for 24 h to which bifidobacteria were added. After 24 h, the dual-species biofilms were exposed to the same carbon sources, and after 3 h, the pH of spent culture media and concentrations of organic acids were measured. Statistical analyses were carried out using ANOVA and Tukey’s test (α = 0.05). Results: A higher pH drop was observed when S. mutans was associated with P. denticolens or S. inopinata, in either planktonic or biofilm cultures, than with S. mutans alone. Bifidobacteria showed a higher pH drop in the presence of raffinose than S. mutans or L. acidophilus. Conclusions: Dual-species biofilms of bifidobacteria and S. mutans produced more acid and greater pH drops than biofilms of S. mutans alone. Clinical relevance: New insights on the complex process of caries pathogenicity contribute to the establishment of preventive and therapeutic measures, in particular in specific cases, such as in early childhood caries.Environmental Engineering Department São José dos Campos Institute of Science and Technology UNESP Univ. Estadual Paulista, Avenida Engenheiro Francisco Jose Longo 777UNIVASF Univ. Federal do Vale do São Francisco, Rua da Alvorada, General DutraAraraquara School of Dentistry UNESP Univ. Estadual Paulista, Rua Humaitá, 1680, CentroSchool of Dentistry Faculty of Medicine and Health University of LeedsDivision of Oral Biology, Wellcome Trust Brenner Building, Level 7, St James’s University Hospital CampusDental Institute King’s College London, Bessemer Rd., Denmark HillOral Biopathology Graduate Program São José dos Campos Institute of Science and Technology, Avenida Engenheiro Francisco Jose Longo 777Environmental Engineering Department São José dos Campos Institute of Science and Technology UNESP Univ. Estadual Paulista, Avenida Engenheiro Francisco Jose Longo 777Araraquara School of Dentistry UNESP Univ. Estadual Paulista, Rua Humaitá, 1680, CentroUniversidade Estadual Paulista (Unesp)Univ. Federal do Vale do São FranciscoUniversity of LeedsDivision of Oral BiologyKing’s College LondonSão José dos Campos Institute of Science and Technologyde Matos, Bruno Mello [UNESP]Brighenti, Fernanda Lourenção [UNESP]Do, ThuyBeighton, DavidKoga-Ito, Cristiane Yumi [UNESP]2018-12-11T17:23:12Z2018-12-11T17:23:12Z2017-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1769-1776application/pdfhttp://dx.doi.org/10.1007/s00784-016-1958-1Clinical Oral Investigations, v. 21, n. 5, p. 1769-1776, 2017.1436-37711432-6981http://hdl.handle.net/11449/17694610.1007/s00784-016-1958-12-s2.0-849887261832-s2.0-84988726183.pdf606563636771738265435631614034210000-0002-4470-51710000-0002-2416-2173Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengClinical Oral Investigations0,9860,986info:eu-repo/semantics/openAccess2024-01-22T06:21:13Zoai:repositorio.unesp.br:11449/176946Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-22T06:21:13Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Acidogenicity of dual-species biofilms of bifidobacteria and Streptococcus mutans |
title |
Acidogenicity of dual-species biofilms of bifidobacteria and Streptococcus mutans |
spellingShingle |
Acidogenicity of dual-species biofilms of bifidobacteria and Streptococcus mutans de Matos, Bruno Mello [UNESP] Acidogenicity Bifidobacteria Biofilms Dental caries |
title_short |
Acidogenicity of dual-species biofilms of bifidobacteria and Streptococcus mutans |
title_full |
Acidogenicity of dual-species biofilms of bifidobacteria and Streptococcus mutans |
title_fullStr |
Acidogenicity of dual-species biofilms of bifidobacteria and Streptococcus mutans |
title_full_unstemmed |
Acidogenicity of dual-species biofilms of bifidobacteria and Streptococcus mutans |
title_sort |
Acidogenicity of dual-species biofilms of bifidobacteria and Streptococcus mutans |
author |
de Matos, Bruno Mello [UNESP] |
author_facet |
de Matos, Bruno Mello [UNESP] Brighenti, Fernanda Lourenção [UNESP] Do, Thuy Beighton, David Koga-Ito, Cristiane Yumi [UNESP] |
author_role |
author |
author2 |
Brighenti, Fernanda Lourenção [UNESP] Do, Thuy Beighton, David Koga-Ito, Cristiane Yumi [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Univ. Federal do Vale do São Francisco University of Leeds Division of Oral Biology King’s College London São José dos Campos Institute of Science and Technology |
dc.contributor.author.fl_str_mv |
de Matos, Bruno Mello [UNESP] Brighenti, Fernanda Lourenção [UNESP] Do, Thuy Beighton, David Koga-Ito, Cristiane Yumi [UNESP] |
dc.subject.por.fl_str_mv |
Acidogenicity Bifidobacteria Biofilms Dental caries |
topic |
Acidogenicity Bifidobacteria Biofilms Dental caries |
description |
Objective: The aim of this study was to evaluate the acidogenicity of dual-species biofilms of bifidobacteria and Streptococcus mutans. Materials and methods: The following strains were tested: Bifidobacterium dentium DSM20436, Parascardovia denticolens DSM10105, and Scardovia inopinata DSM10107. Streptococcus mutans UA159 and Lactobacillus acidophilus ATCC4356 were used as control. Bifidobacteria were studied planktonically as they were not able to form monospecies biofilm, they were grown in biofilms associated with S. mutans. Endogenous polysaccharide reserves of cultures at log phase were depleted. Standardized suspensions of the microorganisms were incubated in growth media supplemented with 10 mM glucose, lactose, raffinose, glucose, or xylitol. S. mutans biofilms were grown on glass cover slips for 24 h to which bifidobacteria were added. After 24 h, the dual-species biofilms were exposed to the same carbon sources, and after 3 h, the pH of spent culture media and concentrations of organic acids were measured. Statistical analyses were carried out using ANOVA and Tukey’s test (α = 0.05). Results: A higher pH drop was observed when S. mutans was associated with P. denticolens or S. inopinata, in either planktonic or biofilm cultures, than with S. mutans alone. Bifidobacteria showed a higher pH drop in the presence of raffinose than S. mutans or L. acidophilus. Conclusions: Dual-species biofilms of bifidobacteria and S. mutans produced more acid and greater pH drops than biofilms of S. mutans alone. Clinical relevance: New insights on the complex process of caries pathogenicity contribute to the establishment of preventive and therapeutic measures, in particular in specific cases, such as in early childhood caries. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-06-01 2018-12-11T17:23:12Z 2018-12-11T17:23:12Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00784-016-1958-1 Clinical Oral Investigations, v. 21, n. 5, p. 1769-1776, 2017. 1436-3771 1432-6981 http://hdl.handle.net/11449/176946 10.1007/s00784-016-1958-1 2-s2.0-84988726183 2-s2.0-84988726183.pdf 6065636367717382 6543563161403421 0000-0002-4470-5171 0000-0002-2416-2173 |
url |
http://dx.doi.org/10.1007/s00784-016-1958-1 http://hdl.handle.net/11449/176946 |
identifier_str_mv |
Clinical Oral Investigations, v. 21, n. 5, p. 1769-1776, 2017. 1436-3771 1432-6981 10.1007/s00784-016-1958-1 2-s2.0-84988726183 2-s2.0-84988726183.pdf 6065636367717382 6543563161403421 0000-0002-4470-5171 0000-0002-2416-2173 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Clinical Oral Investigations 0,986 0,986 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1769-1776 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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